Leica TCS SP8 SMD Manuals

Manuals and User Guides for Leica TCS SP8 SMD. We have 1 Leica TCS SP8 SMD manual available for free PDF download: User Manual

Leica TCS SP8 SMD User Manual (270 pages)

for FCS, FLIM and FLCS

Brand: Leica | Category: Laboratory Equipment | Size: 3.31 MB

Copyright
3
Table of Contents
5
Contents
5
1 About this User Manual

15
- Additional Documentation
  16
2 Intended Use

17
3 Liability and Warranty

19
- Important Information for Operators and Users
  19
4 Meaning of the Warning Messages in the Manual

21
5 General Safety Notes

23
- Commissioning and Use
  23
- Modifications to the System
  24
- Safety Devices and Safety Labels
  24
- Laser Safety
  24
- Electrical Safety
  26
- Contact with Liquids
  26
- Malfunction of the System
  26
6 Additional Notes on Handling the System

29
- Location
  29
- Using the Software
  29
- Protecting the System
  30
- Objectives
  31
7 System Overview and Properties

33
8 Ambient Conditions

45
9 SMD Components

51
- Hardware Components and Software Licenses
  51
- FCS/FCCS Application Area
  51
- Special SMD Hardware
  51
- Software License
  51
- FLIM Application Area
  52
- Special SMD Hardware
  52
- Software License
  52
- Application Area FCS/FCCS, FLIM, FLCS, Gated FCS
  53
- Special SMD Hardware
  53
- Software License
  53
- Beam Path
  53
- Detection Units from Picoquant
  54
- Leica APD Detector Unit
  55
- General Precautionary Measures for Using APD Detector Units
  56
- Changing the Fuse
  56
- Safety Shutoff
  56
- Trigger Unit
  56
- Laser Coupling Unit (LCU)
  57
- Attenuation Unit
  58
- Other Picoquant Components
  59
10 Laser

61
11 Safety Features

67
12 Safety Labels on the System

81
- Compact Supply Unit (Only for FLIM)
  81
- Flexible Supply Unit
  82
- Inverted Microscope
  83
- Upright Microscope
  84
- Mirror Housing
  85
- Cover for Replacement Flange
  86
- Transmitted Light Detector (Tld)/Reflected Light Detector (RLD)
  87
- Scan Head
  87
- White Light Laser
  89
- External UV Laser
  89
- MP Beam Coupling Unit
  90
- Other External Lasers
  90
13 Switching on the System

91
14 Las Af

103
- Starting las AF
  103
- Structure of the Graphical User Interface
  104
- Design of the FLIM Wizard in las AF
  107
- Design of the FCS Wizard in las AF
  110
- LAS AF Online Help
  113
- Structure of Online Help
  113
- Accessing Online Help
  113
- Selecting the Language for Online Help
  113
- Using Online Help
  114
- Full-Text Search with Logically Connected Search Terms
  115
15 Selecting the Laser

117
- Activate Laser as the Excitation Source in the Configuration Menu
  117
- Using Continuous Wave VIS Lasers
  118
- Using MP Lasers
  119
- Using Pulsed VIS Lasers
  121
- Using Pulsed UV Lasers
  124
- Using a Pulsed White Light Laser
  124
16 FLIM Data Acquisition

127
17 Summarized Manual for FLIM Experiments

163
- Prerequisites
  163
- Selecting Position for the FLIM Measurement
  163
- Changing from Continuous to Pulsed Excitation
  163
- Changing from Internal Detection on the SP8 to External TCSPC Detectors
  164
- Using Internal SP FLIM Detection
  164
- Using FLIM Detectors at the NDD Position (Hyd RLD)
  164
- Setting Suitable Scan Parameters
  165
- Optimizing the Photon Count Rate
  165
- Selecting the Correct Laser Repetition Rate
  168
- Starting FLIM Data Acquisition
  170
- Resulting Raw Data File and Documentation
  170
- Measuring the Instrument Response Function (IRF)
  172
- Preparing IRF Measurements
  172
- Estimating the IRF
  172
- With Reflection Mode
  172
- With Fluorescence Mode
  173
- With SHG
  173
- Running IRF Measurement
  174
- Remarks
  176
- Ad-Hoc-Inspection of a Specimen
  176
- Bidirectional Scanning
  176
- Setting the Laser Intensity of the Diode Lasers
  176
- Sensitivity of the Fluorescence Detection
  176
- Optimum Lifetime Information
  176
18 L)CS Data Acquisition

177
19 Summarized Manual for FCS or Other Point Measurements

209
20 Changing the Specimen

213
- Changing the Specimen on an Upright Microscope
  213
- Changing the Specimen on an Inverted Microscope
  213
21 Changing the Objective

215
22 Piezo Focus on Upright Microscope

217
23 Changing the Filter Cube

219
24 Changing Detector Cable Connections on the Scan Head and Router When

221
- Using Hyd-RLD
  221
- Hardware Trees
  222
- MP on FCS FLIM 2 APD
  223
- MP off FCS FLIM 2 APD
  223
- MP on Hyd RLD FLIM
  223
- Connect and Use Detectors
  224
- Using SP FLIM PMT
  224
- Using MPD Apds
  224
- Using Hyd-RLD
  225
25 Switching off the System

229
- System with Flexible Supply Unit
  229
- System with Compact Supply Unit (Only for FLIM)
  233
26 Care and Cleaning

239
- Cleaning Surfaces
  239
- Cleaning the Optical System
  239
- Cleaning Immersion Lenses
  240
- Care
  240
27 Repairs and Service Work

241
28 Maintenance

243
- Having Coolant Replaced
  243
29 Disassembly and Transport

245
30 Disposal

247
31 Troubleshooting

249
32 Contact

253
33 Recommended Literature

255
34 Abbreviations

257
35 Appendix

259
- Patents
  259
- Safety Data Sheets from Third-Party Manufacturers
  259
- Compliance
  265
- People's Republic of China
  267

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More Leica Manuals

Leica TCS SP8 SMD Manuals

Leica TCS SP8 SMD User Manual (270 pages)

Table of Contents

1 About this User Manual

2 Intended Use

3 Liability and Warranty

4 Meaning of the Warning Messages in the Manual

5 General Safety Notes

6 Additional Notes on Handling the System

7 System Overview and Properties

8 Ambient Conditions

9 SMD Components

10 Laser

11 Safety Features

12 Safety Labels on the System

13 Switching on the System

14 Las Af

15 Selecting the Laser

16 FLIM Data Acquisition

17 Summarized Manual for FLIM Experiments

18 L)CS Data Acquisition

19 Summarized Manual for FCS or Other Point Measurements

20 Changing the Specimen

21 Changing the Objective

22 Piezo Focus on Upright Microscope

23 Changing the Filter Cube

24 Changing Detector Cable Connections on the Scan Head and Router When

25 Switching off the System

26 Care and Cleaning

27 Repairs and Service Work

28 Maintenance

29 Disassembly and Transport

30 Disposal

31 Troubleshooting

32 Contact

33 Recommended Literature

34 Abbreviations

35 Appendix

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