2-Laser Pie (405, 470, 640 Nm) - Leica TCS SP8 SMD User Manual

FLIM Data Acquisition
The following window is displayed:
Figure 130:
4. In the Acquisition field, you can change the resolution under resol. Please confirm the
settings by selecting apply. To cover the whole range between two consecutive laser
pulses at the maximum resolution possible, the following settings are recommended:
Table 20:
The default is a setting of 16 ps suitable for a laser repetition rate down to 20 MHz.
16.2.5

2-Laser PIE (405, 470, 640 nm)

PIE (pulsed interleaved excitation) means that two lasers pulse alternatingly. This method is
used to identify crosstalk and cross excitation and remove them from the signal. In the SMD
system, this option is available for certain combinations of pulsed diode lasers (405+470,
405+640, 470+640). Exactly two diode lasers have to be selected in order to activate 2-laser
PIE. If this is not the case, the corresponding radio button is grayed out.
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Oscilloscope window in SymPhoTime
Laser frequency
80 MHz (MP laser, white light laser)
40 MHz (pulsed diode laser, white light laser)
20 MHz (pulsed diode laser, white light laser)
10 MHz (pulsed diode laser, white light laser)
5 MHz (pulsed diode laser)
Recommended resolution for data acquisition with different laser frequencies
Resolution of data acquisition
4 ps
8 ps
16 ps
32 ps
64 ps