User Manuals: BIO RAD CFX384 PCR Detection System

Manuals and User Guides for BIO RAD CFX384 PCR Detection System. We have 1 BIO RAD CFX384 PCR Detection System manual available for free PDF download: Instruction Manual

BIO RAD CFX384 Instruction Manual

BIO RAD CFX384 Instruction Manual (158 pages)

Real-Time PCR Detection Systems

Brand: BIO RAD | Category: Measuring Instruments | Size: 14.56 MB

Table of Contents

Bio-Rad Resources
3
Writing Conventions Used in this Manual
3
Safety and Regulatory Compliance
4
Table of Contents
6
Chapter 1. System Installation

10
- Unpacking the Optical Reaction Module
  10
- System Requirements
  10
- System Overview
  11
- Setting up the System
  12
- Installing CFX Manager Software
  15
- Running Experiments
  18
Chapter 2. Introduction to CFX Manager Software

20
- Main Software Window
  20
  - Status Bar
    21
  - Menu Bar
    21
- Startup Wizard
  24
- Detected Instruments Pane
  25
- Instrument Properties Window
  27
- Software Files
  29
- Tips and Tricks
  29
Chapter 3. Running Experiments

30
- Experiment Setup Window
  30
- Protocol Tab
  31
- Plate Tab
  32
- Start Run Tab
  33
- Run Details Window
  34
- Instrument Summary Window
  37
Chapter 4. Protocols

40
- Protocol Editor Window
  40
- Protocol Editor Controls
  42
- Temperature Control Mode
  46
- Protocol Autowriter
  46
Chapter 5. Plates

50
- Plate Editor Window
  50
- Select Fluorophores Window
  54
- Experiment Settings Window
  57
- Well Groups Manager Window
  59
- Plate Spreadsheet View Window
  60
Chapter 6. Stand-Alone Operation

62
- Control Panel
  62
- Main Menu
  63
- Experiment Setup
  64
- Exporting Data for Analysis
  70
- Creating a Data File
  73
- Setting up Email
  74
Chapter 7. Data Analysis Overview

78
- Data Analysis Window
  78
- Data Analysis Menu Bar
  80
- Quantitation Tab
  81
- Data Analysis Settings
  82
- Well Selectors
  84
- Charts
  87
- Spreadsheets
  88
Chapter 8. Data Analysis Windows

90
- Quantitation Tab
  90
- Quantitation Data Tab
  94
- Melt Curve Tab
  96
- Melt Curve Data Tab
  98
- End Point Tab
  100
- Allelic Discrimination Tab
  102
- QC Tab
  104
- Reports for Data Files
  106
Chapter 9. Gene Expression Analysis

110
- Gene Expression
  110
- Plate Setup for Gene Expression Analysis
  111
- Gene Expression Tab
  111
- Experiment Settings Window
  116
- Gene Study
  118
- Gene Study Data Spreadsheet
  121
- Gene Study Report Window
  122
- Frequently Asked Questions
  123
- Gene Expression Calculations
  124
Chapter 10. Users and Preferences

130
- Log in or Select User
  130
- User Preferences Window
  131
- Email Tab
  132
- Files Tab
  133
- Protocol Tab
  133
- Plate Tab
  134
- Data Analysis Tab
  135
- Gene Expression Tab
  136
- QC Tab
  137
- User Administration
  138
Chapter 11. Resources

140
- Calibration Wizard
  140
- Instrument Maintenance
  142
- Application Log
  144
- Software Help Tools
  144
- Troubleshooting
  145
- Instruments, Parts, and Accessories
  147
- References
  148
Index

150

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