User Manuals: BD FACSCalibur Flow Cytometry System

User Manuals: BD FACSCalibur Flow Cytometry System

Manuals and User Guides for BD FACSCalibur Flow Cytometry System. We have 1 BD FACSCalibur Flow Cytometry System manual available for free PDF download: Instructions For Use Manual

BD FACSCalibur Instructions For Use Manual

BD FACSCalibur Instructions For Use Manual (238 pages)

flow cytometer

Brand: BD | Category: Measuring Instruments | Size: 7.38 MB

Table of Contents

Table of Contents
5
About These Instructions

11
- Conventions
  11
Chapter 1: Introduction

13
- System Components and Theory of Operation
  14
  - Cytometer Components
    15
  - Loader (Optional)
    20
  - Power Controls
    22
- System Requirements
  23
Chapter 2: BD Facstation Software

25
- BDPAC Software
  27
- BD Cellquest Pro Software
  28
  - BD Cellquest Pro Files
    30
- BD Facscomp Software
  31
  - BD Facscomp Preferences
    33
  - BD Facscomp Files
    34
- BD Multiset Software
  39
  - BD Multiset Preferences
    41
  - BD Multiset Tools
    43
  - BD Multiset Files
    45
- BD HLA-B27 Software
  47
  - BD HLA-B27 Preferences
    49
  - BD HLA-B27 Files
    50
- BD FACS Loader Software
  51
  - Worklist Manager Preferences
    53
  - Worklist Manager Files
    53
Chapter 3: Starting up

55
- Filling the Sheath Reservoir
  56
  - Priming the Fluidics
    58
- Emptying the Waste Reservoir
  58
Chapter 4: Instrument QC

61
- Setting up for QC
  62
  - Editing Target Values
    65
- Running BD Facscomp Software
  66
  - Acquiring with the Loader
    66
  - Acquiring Manually
    69
  - Viewing Results
    77
- Optimizing Settings
  79
  - Downloading Instrument Settings
    82
- Logging QC Results
  83
Chapter 5: Running Samples Manually

85
- Running Samples with BD Cellquest Pro Software
  86
  - Setting up an Experiment Document
    86
  - Optimizing Settings
    90
  - Acquiring Data
    99
  - Saving Data
    110
  - Analyzing Data
    113
- Running Samples with BD Multiset Software
  121
  - Setting up
    121
  - Acquiring Data
    130
  - Reviewing Lab Reports
    133
  - Analyzing Data
    137
- Running Samples with BD HLA-B27 Software
  139
  - Setting up
    139
  - Acquiring Data
    144
  - Analyzing Data
    146
Chapter 6: Running Samples with the Loader

149
- Preparing an Acquisition Document
  150
- Setting up BD Worklist Manager Software
  151
  - Setting up Assay Settings
    153
  - Running BD Facscomp Software
    155
  - Saving the Worklist
    155
- Entering Sample Information
  156
  - Assigning Racks
    158
- Running Samples
  160
  - Pausing Acquisition
    163
  - Rerunning a Sample
    164
Chapter 7: Shutting down

165
- Daily Shutdown with the Loader
  166
Chapter 8: Maintenance

169
- Scheduled Maintenance
  170
  - Decontaminating the Fluidics
    171
  - Changing the Sheath Filter
    173
- Unscheduled Maintenance
  175
  - Changing the Bal Seal
    176
  - Changing the Sample O-Ring
    178
- Loader Maintenance
  179
  - Checking Tube Lifter Calibration
    180
  - Verifying Connection Ports
    181
  - Replacing the Loader Seal
    182
  - Cleaning the Tube Lifter
    184
  - Modifying the Cytometer for Non-Loader Use
    186
Chapter 9: Troubleshooting

189
- Instrument Troubleshooting
  190
- Acquisition Troubleshooting
  191
- BD Facscomp Troubleshooting
  195
  - PMT Adjustment
    195
  - Time Delay Setup
    197
  - Fluorescence Compensation
    198
  - Sensitivity Test
    199
  - HLA-B27 Calibration
    199
- BD Multiset Troubleshooting
  200
  - Error Codes
    200
  - General BD Multiset Software
    201
  - BD Multiset Results
    203
- Loader Troubleshooting
  208
  - Worklist Manager Troubleshooting
    210
Appendix A: Supplies and Replacement Parts

211
- Instrument Supplies
  212
  - Accessory Kit
    212
  - Other Replacement Parts
    213
- Consumables
  214
  - Instrument Setup
    214
  - Reagents
    215
  - Labware
    215
Appendix B: Technical Specifications

217
- Cytometer Specifications
  218
  - Environment
    219
  - Performance
    219
  - Optics
    220
  - Fluidics
    221
  - Signal Processing
    222
  - Loader Specifications
    222
- Connections
  223
Appendix C: Performance Data

225
- Accuracy
  226
- Precision
  227
- Linearity
  229
Index

231

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