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DAKEWE CT520 User Manual

Cryostat microtome
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CT520
Cryostat Microtome
User's Manual
V2.1 English - May. 2024

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Summary of Contents for DAKEWE CT520

  • Page 1 CT520 Cryostat Microtome User’s Manual V2.1 English - May. 2024...
  • Page 2 Address of manufacturer: Floor 5, Building B, No.2 Luhui Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen, China. Tel: 0755-26413421 Name of after-sales service provider: Dakewe (Shenzhen) Medical Equipment Co., Ltd. Address of after-sales service provider: Floor 5, Building B, No.2 Luhui Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen, China.
  • Page 3 Introduction Dear user: Thank you for choosing our product! In order to present an overall understanding of our product and facilitate your use, we specially provide these Instructions for Use, covering the structural features, technical features, instructions for use, fundamental troubleshooting and maintenance of the instrument. The Instructions for Use are an essential guide for you to use the instrument.
  • Page 4 Instructions for Use. This document is protected by copyright law, and all copyrights on it are owned by Dakewe (Shenzhen) Medical Equipment Co., Ltd. Any reproduction of the text and illustrations (or any part...
  • Page 5 Table of Contents 1. Information............................1 1.1. Symbols and meanings.......................1 1.2. Use of the instrument......................... 5 1.3. Personnel qualifications......................5 1.4. Instrument model........................5 1.5. Contraindication......................... 5 2. Safety..............................6 2.1. Safety notes..........................6 2.2. Warnings.............................6 2.2.1. Warning - symbols on the instrument................7 2.2.2.
  • Page 6 4.1. Installation requirements......................17 4.2. Tools required for unpacking....................17 4.3. Unpacking..........................18 4.4. Handling of the instrument...................... 18 4.5. Verification of accessories......................19 4.6. Install the handwheel........................19 4.7. Install the blade holder......................19 4.8. Install the heat extractor......................20 4.9. Electrical connection........................20 5.
  • Page 7 6.4.1. Component monitoring....................38 6.4.2. Events..........................39 6.4.3. Errors..........................40 6.4.4. User manual....................... 40 6.5. Software update........................41 7. Sectioning............................42 7.1. Sectioning preparations......................42 7.1.1. Blade holder assembly....................42 7.1.2. Fix the specimen head....................45 7.1.3. Freezing specimens....................... 46 7.1.4. Prepare tools........................47 7.2. Install a blade........................... 47 7.3.
  • Page 8 11.3. Service life..........................60 11.4. Scrap and disposal........................60 Appendix: Hazardous substance information..................61 Appendix: Electromagnetic compatibility performance index...............62...
  • Page 9 The power is off Biohazard warning (black line on yellow background) This warning symbol indicates the surface of the instrument that will be hot during operation; Avoid direct contact to prevent burns Electrical hazard Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 10 Date of manufacture Manufacturer Unique device identification Do not stack Maximum of 2 floors (for accessories cases only) Indicates the correct upright position of the packing case. Fragile; handle with care Avoid dampness and keep dry Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 11 Authorized representative in the European Community Importer The CE marking is the manufacturer’s declaration that the medical product meets the requirements of the applicable EC directives and regulations Name and Address of Switzerland responsible person Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 12 The UKCA (UK Conformity Assessed) marking is a UK product marking used for certain goods, including medical devices, being placed on the Great Britain market (England, Wales and Scotland) Name and Address of UK responsible person Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 13 1 Information 1.2. Use of the instrument The Dakewe CT520 Cryostat microtome is used to freeze and section human and animal body tissue. It can be widely used for preparing thin sections of samples of varying hardness for use in pathological diagnosis, analysis and research in hospitals, medical colleges by legal medical experts and propagation institutes.The instrument is designed to produce frozen sections for biological,...
  • Page 14 The safety notes described in this chapter must always be observed. Be sure to read these instructions even if you are already familiar with the operation and use of other Dakewe products. The instrument has been designed and constructed with the latest state-of-the-art technology and according to recognized standards and regulations with regard to safety technology.
  • Page 15 Always wear safety goggles when sectioning fragile specimens! Specimens may be fragmented! Make sure that no liquid enters the instrument during operation! Immediately clean the embedding agent that falls on the floor or injury due to slippery floor may occur! Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 16 Failure to follow this may lead to the damage of the specimen. 2.2.4. Warning - cleaning and maintenance Only qualified maintenance personnel authorized by Dakewe can access the internal components of the instrument for maintenance and repair! Never try to catch falling accessories as it may cause injury!
  • Page 17 Transport the instrument to the location where it will be placed. After positional adjustment, adjust the adjustable feet to support the instrument on the floor, so that it is fixed in place. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 18 Switch on the instrument by the switch on the back.  Use only power cords supplied with DAKEWE or certified power cords.  The handwheel must be locked when the instrument is not in use.
  • Page 19 20 specimens at a time (including 2 Peltier elements). The multi-color specimen discs assist specimen identification; (10) The instrument has rapid cooling. The freeze shelf is equipped with two Peltier elements, with a Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 20 (20) The instrument has an ergonomic arc design and convenient handwheel operation, improving user experience and avoiding fatigue; (21) The instrument has a slide storage area providing easy access to marked slides; (22) The intelligent compressor working algorithm ensures efficient cooling and meanwhile maintain low-noise operation. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 21 8. Feet 3. Window 6. Handwheel 9. Caster Figure 3- 2 Diagram of the cryochamber 10.Heat extractor 12. Blade holder 14. No. 5 brush 11. Specimen head 13. No. 4 line drawing pen 15. Brush Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 22 5 μm increment Trimming thickness 50 - 100μm 10 μm increment 100 - 600μm 20 μm increment Retraction value 0-100μm 5.0 μm increment Horizontal feed 30 ± 1 mm Vertical stroke 62 ± 1 mm Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 23 Forward and backward movement of specimen head, switching Control panel between sectioning and trimming, and temperature adjustment Handwheel Can be lock at 12 o'clock/6 o'clock position Temperature control Separate adjustment of freeze shelf/specimen head/blade holder, three Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 24 Dimensions and weight Parameter 830 (including handwheel) x 910 (including rear grip) x 1,300 mm Dimensions (Width × depth × height) 700 (excluding handwheel) x 830 (excluding grip) x 1,300 mm Weight (without accessories) About 185kg Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 25  The location must be well ventilated, and no fire source is allowed. 4.2. Tools required for unpacking Figure4- 1 Unpacking Tools 1. Electric screwdriver 2. Adjustable wrench 3. Utility knife 4. Allen wrench Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 26 Move the instrument from the base of the crate to the floor, pull the grip at the rear of the instrument to move the instrument to the target position. After the instrument is in position, turn the feet clockwise so that they support the instrument stably on the floor. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 27 4.7. Install the blade holder Take the blade holder out of the accessory box. Figure a Snap the blade holder from the front Figure b Installation location of the blade holder Figure4- 4 Install the Blade Holder Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 28 Figure4- 6 Connect of Mains Cord The instrument must be connected to a grounded mains socket. Place the instrument at a place that is convenient to operate. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 29 (Figure 5-1-2). After start-up, the display screen lights up (Figure 5-1-1), and the compressors start at the same time. The refrigeration of the freeze shelf, specimen head and blade holder is conducted according to the respetive set temperature. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 30 Click the Switch User, and then select “Administrator” on the popup window (Figure 5-3-3). Click “Confirm”. On the Administrator Login popup, click the password input (Figure 5-3-4), enter the password using the virtual keyboard, and click “Confirm” to switch to the administrator user. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 31 (6) The sleep button is to the right of the lock screen button. Click the sleep button to enter the sleep mode. At this time, the screen turns to the lowest brightness, the freeze shelf, specimen head and Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 32 Clear: Section times sum and section thickness sum do not reset everyday. Only by clicking the Clear button (Figure 5-5-5) will the Section times sum and section thickness sum be cleared. Figure5- 5 Sectioning settings on the Main Interface Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 33 Specimen head: As shown (in Figure 5-7-4), the current temperature of the specimen head is Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 34 Preset Temperature Module Quick Select: As shown in (Figure 5-9), three presets (Regular/Thyroid/Fatty) of temperature can be stored and selected easily. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 35 Figure a the preset name area Figure b Enter a name via the soft keyboard Figure c Confirm after editing Figure d Edited preset name on the main interface Figure5- 10 Edit preset name Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 36 Click “Set” button to record the current position of the specimen head (Fig. 5-11-1). A memory position is then recorded. The “Set” button will change to “Clear” when a memory position is se t(Figure 5-11-2). Click “Move” and the specimen head will return to the recorded memory position. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 37 OK to start defrosting. The defrosting icon on the interface will be highlighted.There is a 25-minute countdown, at the end of which the defrost icon returns to the unopened state and the cooling mode is automatically restored. Figure5- 13 Defrosting Prompt Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 38 A W307 prompt will show on the main interface when the ambient temperature exceeds 35℃. The cooling capacity of the instrument is compromised under the condition. Please turn on the air conditioning system in the room to lower the temperature. The W307 prompt disappears when the Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 39 These buttons can be used to adjust thickness and temperature; Specimen head slow backward movement; Specimen head slow forward movement; Specimen head fast backward movement till the end; Specimen head fast forward movement; Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 40 Touch sound: click to turn on/off touch sound. Language: drop down to choose a system language. Time settings: under the permission of the admin, click time settings to adjust the date and time, and Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 41 Step 3: configurate the start time or the end time and click OK (Figure 6-3-4). Click the OK button on the edit pop-upto save the changes. Step 4: check the box beside the chosen time period (Figure 6-3-5). Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 42 Step 4: configurate the start time or the end time and click OK (Figure 6-4-4) . Click the OK button on the edit pop-up to save the changes. Step 5: Check the box beside the chosen time period (Figure 6-4-5). Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 43 ④Please select Standard Defrosting in the mode selection after a deep defrosting procedure completes. ⑤ To restore to the default defrosting time, click the Restore to Default button in the upper right corner. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 44 ③ To restore to the default activation time, click the Restore to Default button in the upper right corner. ④ The longest activation duration can be set is 20 hours. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 45 10 min. Click "-" and "+" to adjust rapid cooling duration within the range of 5 - 30 min. Functions in Preferences can only be adjusted by admins, and standard users can only view them. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 46 6.4. Maintenance 6.4.1. Component monitoring Figure 6- 8 Component Monitoring Record the component name, usage times, usage duration and operation on the component records interface. The admin and the standard users see different contents. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 47 Insert a USB 2.0 port driver, and click the Export button (Figure 6-9-1) to export the logs. The exported logs will be saved in the output folder in the USB drive (Figure 6-10). Figure 6- 10 Exported Logs Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 48 Error recordcan only be viewed by the admin, and cannot be viewed by standard users. 6.4.4. User manual Instructions, common error handling, precautions for use and other documents are included inside the user manual to assist users to use the equipment properly. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 49 Create an update folder under the root directory of the USB drive, put the update file into this folder, insert the USB drive into the instrument's USB 2.0 port, click the Update button that lights up on the update interface, and wait for the update to be completed. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 50 Rotate the "blade holder lateral shift lever" on the left counterclockwise, and move the blade holder laterally to make use of the whole blade for sectioning. Rotate the lever clockwise to clamp the blade holder. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 51  Use a hexl wrench to loosen the "screw for parallelism between the cutting edge and the anti-roll guide";  Adjust the angle of the anti-roll guide so that the edge of the anti-roll guide is parallel to the cutting edge of the blade; Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 52 10°. (6) Insert or switch to a high profile blade The blade holder on CT520 cryostat is compatible for both high profile and low profile blades. Follow these steps:  Remove the blade rest (Figure7-4-a);...
  • Page 53 Press the specimen head clamp lever down to clamp the specimen disc to the specimen head.  Rotate the "specimen head orientation lever" to loose the clamp of the specimen head, pull the "orientation screws" to adjust the angle of the specimen. Clamp the specimen head. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 54 The freeze shelf is on the left side of the cryochambe with 20 stations, including 2 Peltier element for rapid freezing and 18 freezing stations. The temperature of the freezing shelf is always lower than that of thecryochamber. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 55 7.2. Install a blade 1) Prepare a blade 2) Unfold the anti-roll guide 3) Insert the blade from the right of the blade holder 4) Tighten the blade clamp lever Figure 7- 8 Install a blade Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 56 Spin the handwheel to start trimming. A recommended trimming thickness is 20 ~ 30 μm；  Trim the specimen to the desired surface, switch to section mode and start sectioning. Figure 7- 9 Trimming Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 57 4. Contrary to the general paraffin block section, for the frozen section, it is better to cut the short side of the tissue instead of the long side, that is, to make the line of contact between the tissue and the Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 58 7 Sectioning sectioning blade shorter. Figure 7- 10 Sectioning Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 59  Use a brush to remove all section wastes;  Take out the open waste tray, brush holder and blade holder, and then clean and disinfect them. Notes: Before restarting the instrument, checked that all parts in the cryochamber are completely dry. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 60  Clean the blade holder and rotary tool apron, especially the contact surfaces between the two;  Dry the parts, apply low-temperature lubricating oil on the contact surface between the blade holder and the rotary tool apron;  Install and restore the blade holder in sequence; Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 61 Notes: Observe the liquid level frequently and empty the waste liquid bottle in time; The waste must be treated in a correct and effective way. Relevant regulations on waste water treatment must be observed. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 62 Slow tissue freezing speed Foreign objects between the specimen Wipe specimen disc and freeze shelf while the freeze shelf disc and the freezing shelf leading to before freezing a specimen temperature is consistent poor heat transfer Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 63 Strips in sections Tissue too thick and detach from the Section thickess recommend 5 - 10 μm specimen disc Increase section thickness or decress Tissue even and hard surface area of the specimen if necessary Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 64 1.Set proper specimen head temperature (refer to User manual) Specimen too cold 2.Section when the specimen is properly froze； Fractured sections 1.Increase specimen head temperature OCT turned brittle when too cold 2、Switch to an OCT with better performance Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 65 OCT applied to cold specimen disc Apply OCT to room temperature disc leading to poor adherance Tissue fall off Trim under trimming mode with Trimming thickness setting too large thickness between 20 to 30 μm Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 66 After the fault occurs, please troubleshoot the fault according to the temporary solution. If the above solution fails to remove the fault, please contact the technical engineer of DAKEWE based on the severity of incident; "Immediately" means that the microtome completely stops working, and the engineer must intervene;...
  • Page 67 Breast fat -45℃ -30℃ -40℃ -25℃~-30℃ The temperature given in the above table is a summary of long-term experience, but these are only approximate values, because specific adjustments may be required for actual organizations. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 68 11 Warranty and service 11. Warranty and service 11.1. Warranty Dakewe employs a comprehensive quality control program based on Dakewe' internal testing standards to ensure that delivered contract products are in good condition and meet all technical specification requirements. The scope of warranty is based on the content of the agreement. If the customer needs special warranty service, please contact the local sales representative or the distributor who sold the instrument.
  • Page 69 The product shall be disposed of in accordance with the laws and regulations on the recycling and reuse of electronic information products. Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 70 It is recommended to assess the electromagnetic environment before using the equipment. Caution: [CT520][Cryostat microtome] is designed and tested according to Class A equipment in CISPR11. In the home environment, this equipment may cause radio interference in which case protective measures must be taken.
  • Page 71 B. In the test, the function or performance is degraded or lost temporarily, but can restore automatically. C. In the test, the function or performance is degraded or lost temporarily, and cannot restore until reference or system operation by the operator Dakewe (Shenzhen) Medical Equipment Co., Ltd.
  • Page 72 Name of manufacturer: Dakewe (Shenzhen) Medical Equipment Co., Ltd. After-sales service unit: Dakewe (Shenzhen) Medical Equipment Co., Ltd. Registered address: Floor 5, Building B, No.2 Luhui Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen,China. Production address: Floor 5, Building B, No.2 Luhui Road, Jinsha Community, Kengzi Street, Pingshan District, Shenzhen,China.