Related Manuals for DAKEWE 6250
Summary of Contents for DAKEWE 6250
- Page 1 Cryostat User’s Manual Applicable Model: 6250 Revised Version V4.1-English Version-June 2021 Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
- Page 2 Before use, be sure to read the User Manual carefully and follow the instructions correctly to the user manual. In addition, if you have any questions during use, contact technical support, we will be glad at your service. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
- Page 3 Manual. This document is protected by copyright law and all copyright in this document belongs to Dakewe (Shenzhen) Medical Equipment Co., Ltd. Copying the text and illustrations (or any part thereof) of...
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Page 4: Table Of Contents
5.1.3 Cold Stage Temperature Setting / Semiconductor Cooling Function ..... 24 5.1.4 Lighting and UV Lamp ..................25 5.1.5 Drying Button ......................25 5. 2 Button Panel ........................26 5. 3 Function Setting and Introduction ..................26 Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... - Page 5 7.7 Use of Waste Tank ......................42 FAQ and Handling ........................42 Section Tissue Temperature Selection Table ................45 10. Warranty and Service ......................46 10.1 Warranty .......................... 46 10.2 Technical Service Information ..................46 10.3 Scrapping and Disposal ....................46 Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
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Page 6: Information
Biohazard warning (black line on yellow background) Sign indicator of using flammable solvents and reagents (black line on yellow background) This warning symbol indicates the instrument surface that will be hot during operation. Avoid direct contact to prevent burns. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... - Page 7 The package must be stored in a dry environment. Recycle in accordance with local laws and regulations. It meets the requirements of EU Directive 98/79/EC for IVD medical devices. Item indication number in the illustration. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
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Page 8: Instrument Use
• Freezer sections: quickly obtaining low temperature and slicing. •Refrigeration parts: including refrigeration compressor and semiconductor cooling fin. The 6250 Semi-automatic Cryostat Microtome is used for rapid freezing of pathological sections of human and animal body tissue. It can be widely used for preparing thin sections of samples of varying hardness for use in pathological diagnosis, analysis and research in hospitals, medical colleges by legal medical experts and propagation institutes. -
Page 9: Safety
This instrument meets the requirements for radiated interference and interference immunity specified in the EMC standards IEC 61326-1:2012 and IEC 61326-2-6:2012 The performance indicators of the instrument are in line with Dakewe's technical requirements of the cryostat. In order to maintain this condition and ensure safe operation, the user must comply with all safety precautions and warnings in this User Manual. -
Page 10: Warning
Do not grasp the handle of the handlewheel or the knob of setting the slice thickness to carry the instrument. It is strictly forbidden to disassemble or modify the protective equipment on the instrument and accessories. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 11: Warning - Operation
The ambient temperature range is 5 °C ~ 30 °C, when the ambient temperature reaches 26 °C or above, the air conditioner should be open for cooling. Air humidity does not exceed 60%. No sunlight for placing. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 12: Instrument Handling
The cooling chamber should be cleaned frequently and kept clean, but especially pay attention that not to be scratched by the blade. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 13: Instrument Components And Specifications
Instrument Components and Specifications Overview Figure 2 Front picture of 6250 Cryostat 1 Display 2 Keyboard 3 Waste barrel 4 Foot cup 5 Power switch 6 Glass door 7 Hand wheel Figure 3 The picture cooling chamber 8 Sample disk... -
Page 14: Technical Parameters
0～600μm 1μm～50μm, with a step of 5μm Trimming step amount 50μm～100μm, with a step of 10μm 100μm～600μm, with a step of 50μm 0~200μm adjustable, with a step of 5μm Sample retraction Sample horizontal stroke 25mm±1mm Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... - Page 15 -60℃（+5℃） of semiconductor rapid cooling area Number of common freezing points Number of semiconductor fast cooling points Refrigerant R449A/400g±5g Cooling capacity 690W Refrigerant and compressor oil should only be replaced by qualified authorized service personnel! Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
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Page 16: First Installation
If the above situation occurs, mark it on the delivery documents and check whether the goods are damaged. Unpacking Tools Figure 4 Tools for unpacking 1. Electric screwdriver 2. Adjustable wrench 3. Utility knife 4. Allen wrench Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 17: Unpacking
The wooden box can be reused. Do not throw away it after unpacking and keep it in a safe place. Check the received parts with the configuration list and order to confirm whether the delivery is complete! If any discrepancies are found, contact your local Dakewe sales office immediately. Dakewe(Shenzhen)Medical Equipment Co,. Ltd... -
Page 18: Accessory Installation
The hand wheel can only be locked at the highest and lowest position and the rest of the position cannot be locked. Hand wheel unlock: reversely push the hand wheel lock lever. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 19: Hand Wheel Block Installation
When the top cover is locked, it is also tightened for 5~8 cycles. Make sure that the screw can support the top cover properly. Continue to tighten the screw, which will cause the top cover to deform or even rupture. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 20: Fixed Card Core
EVA cotton. The chuck core can move up and down normally. 4.4.5 Waste Trough Installation Figure 10 The picture of waste trough Place the waste trough in the body of the universal specimen clamp in the direction of the illustration (Figure 10) Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 21: Blade Holder Installation
(Figure 11-2) Slide to the appropriate position, then rotate the handle to lock the blade holder. 4.4.7 Glass Door Installation Conductive sheet Conductive electrode Figure 12 The procedure of glass door installation Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 22: Power Supply Cable Connection
Check that the tool holder is installed in place and locked on the tool holder mounting plate of the freezer compartment. Put the waste slot, brush, No. 4 hook pen, front brush holder, blade and other utensils into the freezer compartment for pre-cooling. Close the glass door. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 23: Operation
(When the compressor stops for less than 3 minutes and receives the signal to start the compressor again, the compressor will not start immediately, it will be delayed until the stop time reaches 3 minutes before starting). Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 24: Slice And Retouch Setting
2: The compressor is in the startup state. If the above two conditions are not met, the semiconductor refrigeration time will be fixed at 300S, and the countdown will not start,it will automatically turn on when the startup conditions are met. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 25: Lighting And Uv Lamp
6 hours to restart the “drying” function to the working temperature of -20°C. During this period, the equipment cannot be used for frozen sectioning. Please select a suitable time to enable the secondary function. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 26: Button Panel
Figure 17 The picture of keyboard interface 5. 3 Function Setting and Introduction See5.3.1 See5.3.2 See5.3.3 See5.3.4 Figure 18 The picture of functional interface During operational process, the screen enters the function setting interface. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 27: Defrost Setting
The defrost time is not associated with the sleep time, and the unlink icon: is displayed before the time. (After setting the time, press the "Save" button to save, otherwise the setting is invalid.) Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 28: Hibernation Setting
35 minutes, and the icon will be darkened. 2. Indication 2 is the automatic disinfection date setting. From Sunday to Saturday.when click the icon at back of the date, it means to be valid. But when the icon is turned up, it Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 29: Time Reset In The Function Interface
4 hours, it will stop for 3 minutes and then turn on again (if the glass door is opened or the semiconductor refrigeration is on at this time, Time will be postponed). Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 30: System Setting
5.5.2 Slice Retraction Value Setting Press the button“+” and “-” to increase or decrease the retraction stroke. The retraction stroke range is 0--100um. Each time you press and decrease, the value increases or decreases by 5um. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 31: Blade Holder Module
7. Blade pressing plate 3. Blade clamping handle 8. Anti-roll plate’s flip handle 4. Holder locking handle for 9. Anti-freeze hand rubber stopper left-and-right movement 5. Holder locking handle for 10. Blade slicing angle locking screw back-and-forth movement Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 32: Blade Holder Front And Rear Position Adjustment
The microtome blade is extremely sharp! Handle with care! Do not catch the falling blade! Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 33: Anti-Roll Plate Adjustment
The adjustable angle range is 0-10°, then fix the blade slicing angle locking screw. If the slicing effect is not good, the angle can be tested by 1° until it is adjusted well, but the angle cannot be changed more than 10°. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 34: Sample Disk Installation
It is locked to the front of the card core. Loosen the head adjustment lock screw on the core of the chuck, and move the head adjustment handle to adjust the direction of the surface of the specimen, tighten it after selection. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 35: Specimen Cryoforming
During specimen freezing, a heavy hammer can be placed on the specimen surface to accelerate the freezing of the specimen surface and flatten the specimen surface; After the specimen is frozen, insert the carrier into the core of the chuck. Once it is tightened, start slicing. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 36: Sectioning Operation
Adjust to the trimming mode, press the fast forward and slow forward keys to move the specimen closer to the blade, and turn the handwheel to trim. The thickness of the trimming is recommended to be 20 ~ 30μm; Close the anti-roll plate and to slice. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 37: Sectioning
4. The frozen section is opposite to the general paraffin section. The frozen section does not cut the long side of the tissue. It is best to cut the short side of the tissue, that is, the contact line between the tissue and the slicing blade is shorter. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 38: Cleaning And Maintenance
Remove the blade from the blade holder, then put it back to the cartridge. Remove the blade holder to clean up. Remove the specimen from the specimen clamp. Use a dry brush to remove the section waste. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 39: Cleaning The Instrument Enclosure
Clean the condenser as needed Open the condenser sealing baffle on the right side of the fuselage, and use a brush to clean the dust on the condenser fins, or spray with high-pressure airflow. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 40: Take The Slicing Core From The Freezer
When reinstalling the slicer movement, the inside of the movement must be completely dry. Otherwise, the moisture inside the movement will freeze at low temperatures, which will damage the body. Any items taken from the freezer must be thoroughly dried before being put back. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 41: Replacing Component
Before replacing the UV lamp, the main switch of the machine must be switched off and the power plug must be unplugged. If the UV lamp is broken, it must be replaced by a professional. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 42: Use Of Waste Tank
Insufficient sample freezing Set a lower temperature Uneven section Foreign matter attached to the Clean and remove foreign blade or anti-roll plate objects The cutting edge is damaged Switch blade cutting edge position or change blade Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... - Page 43 Anti-roll plate with static Remove static electricity electricity Foreign matter on the edge of Clean the anti-roll plate and Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
- Page 44 Before freezing the tissue, use slice absorbent paper to absorb as much water as possible from the tissue Tissue freezing is too slow semiconductor refrigeration points to freeze tissues; 2. Use heavy hammer correctly to speed up tissue freezing; Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1...
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Page 45: Section Tissue Temperature Selection Table
-15℃~-20℃ Uterine scraping -10℃~-15℃ Cartilage -10℃~-15℃ Caution: The temperature given in the above table is a summary of long-term experience, but these are only approximate values, because specific adjustments may be required for actual organizations. Dakewe(Shenzhen)Medical Equipment Co,. Ltd V4.1... -
Page 46: Warranty And Service
10.1 Warranty Dakewe guarantees that the delivered contract product adopts a comprehensive quality control program based on Dakewe’s internal testing standards and that the product is intact and meets all technical specifications. The scope of the warranty is based on the content of the agreement. For special warranty services, contact your local sales representative or the dealer who sells the instrument. - Page 47 [Manufacturer Name] Dakewe (Shenzhen) Medical Equipment Co., Ltd [After-sales Service Organization] Dakewe (Shenzhen) Medical Equipment Co., Ltd [Registered Address] Room 601, Building 1, Shenzhen Bio-pharmaceutical Innovation Industrial Park, No. 14, Jinhui Road, Kengzi Street, Pingshan New District, Shenzhen [Production Address] Room 601, Building 1, Shenzhen Bio-pharmaceutical Innovation Industrial Park, No.
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