ELITechGroup CYTOPRO 7622 Applications Manual
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ELITechGroup CYTOPRO 7622 Applications Manual

Cytocentrifuge
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CYTOPRO
 
CYTOCENTRIFUGE  
Model   7 622  
 
®
 

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Summary of Contents for ELITechGroup CYTOPRO 7622

  • Page 1 ® CYTOPRO                 CYTOCENTRIFUGE                           applications           manual                 Model  ...
  • Page 2 ® CYTOPRO CYTOCENTRIFUGE Model 7622 Applications Manual 57-2003-01B...
  • Page 3 ©2013 ELITechGroup Inc. All rights reserved. Printed in the United States of America. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means whatsoever, without the prior express written permission of ELITechGroup Inc.

  • Page 4: Table Of Contents

    Table of Contents Section 1 Introduction 1.1 Cytopro Cytocentrifuge Overview ............5 Using this Manual ...............5 Functional Description ..............6 Key Features ................6 Intended Use ................7 Table 1: Cytopro Specifications ...........8 Table 2: Cytopro Rotor Specifications .........8 Table 3: Sample Treatment Options ...........9 Table 4: Explanation of Symbols ..........10 Figure 1: The Cytopro Cytocentrifuge .........11 Figure 2: Instrument Rear Panel ..........11...
  • Page 5 Table of Contents Section 2 Controlling and Customizing Cytocentrifuge Functions (continued) Managing User Access ............25 User Login/Logout ..............26 Changing User Language ............26 Setting the Date and Time ............26 System Log..................27 Accessing Logs ..............27 Exporting Logs ..............27 Controlling Beeper Alerts ............27 Slide and Specimen Tracking ............28 Enable Cyto Slide Tracking ..........28 Enable Manual Entry ............28...

  • Page 6: Cytopro Cytocentrifuge Overview

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Using this Manual This manual provides instructions to install, operate, and maintain the Cytopro Cytocentrifuge. The manual is an important part of the product. Read it carefully and completely before setup and first use of the instrument. If additional accident prevention and environmental protection requirements exist in the country of operation, this manual must be supplemented by appropriate instructions to ensure compliance.

  • Page 7: Functional Description

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview WARNING! If power is lost during cytocentrifugation, the lid remains locked until power is restored. Do not attempt to open the lid while power is off. WARNING! Electrical shock hazard: Do not open this instrument or attempt internal repairs. Refer servicing to qualified service personnel.

  • Page 8: Intended Use

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Intended Use The Cytopro Cytocentrifuge is an in vitro diagnostic medical device for fixing biological cell suspensions on glass microscope slides for cytological examination. The Cytopro can be used with the following cell suspensions: ...
  • Page 9 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Table 1: Cytopro Specifications Category Characteristics Electrical Requirements 100 to 240 VAC @ 50 to 60 Hz Power Consumption 200 Watts maximum Fuses 2.0 Amp time-lag (Type T) (5 x 20 mm) (2 required). Manufactured by Littlefuse (Part No.
  • Page 10 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Table 3: Sample Treatment Options The chart below suggests procedures for various fluids. Refer to the Methods Manual for more detailed information. Methods currently used in other cytocentrifuges will often work in the Cytopro, if the maximum volume of fluid and the run time is adjusted appropriately (see chart).
  • Page 11 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Table 4: Explanation of Symbols Biological Hazards (Biological Risks) Alternating Current (AC) Power ON Power OFF Caution, Consult Accompanying Documents (Attention, see instructions for use) Biological Hazard Symbol Manufacturer’s Representative in Europe Manufactured by Waste Electrical and Electronic Equipment (WEEE) CE Mark, Product meets the essential requirements designated in Annex I of the In Vitro Device Directive (IVDD) 98/79/EC...
  • Page 12 SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Figure 1: The Cytopro Cytocentrifuge 1 – Interactive Touchscreen/Display 2 – Lid 3 – Rotor Rotation Observation Port 4 – Cytopro Rotor Figure 2: Instrument Rear Panel 1 – Model and Serial Number Label 2 –...

  • Page 13: The Cytopro Rotor

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview The Cytopro Rotor The Cytopro rotor holds up to eight sample chamber assemblies, and microscope slides. The rotor operates on the drive hub of the instrument. The self-sealing, autoclavable rotor is easy to load in a biological safety cabinet. The lid seals airtight to contain biological hazards. The low-profile rotor allows easy access during loading.

  • Page 14: Single Sample Chamber

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Figure 4: Front Panel and Touchscreen 1 – Standby/Ready Button 2 – Touchscreen The front panel features an interactive touchscreen display. Refer to Touchscreen and User Interface (Section 1.2, Table 5) for more information. Single Sample Chamber The reusable single chamber features a dual-port sample loading port system that places a 38.5 mm...

  • Page 15: Dual Sample Chambers

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Figure 5: Single Chamber Assembly 1 – Chamber Frame 2 – Cytopad 3 – Chamber Base 4 – Tunnel Port Cap Vent 5 – Sample Well Cap Vent 6 – Cap 7 – Sample Well 8 –...

  • Page 16: Cytopro Magnum® Sample Chambers

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Figure 6: Dual Sample Chamber 1 – Chamber Frame 2 – Cytopad 3 – Chamber Base 4 – Cap Vents 5 – Cap 6 – Sample Wells 7 – Chamber Pressure Rings 8 – Flow Control Rings Cytopro Magnum®...

  • Page 17: Cytopro Absorption Pads

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Figure 7: Cytopro Magnum Chamber 1 – Cytopro Magnum Chamber 2 – Chamber Cap 3 – Suspension Fluid Absorption Chambers (2) 4 – Sealing Gasket Cytopad® Absorption Pads Cytopads (standard chambers only) absorb suspension fluid and allow sample cells to sediment onto the microscope slide.

  • Page 18: Chamber Frame

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Chamber Frame Chamber frames accept either single or dual replacement pads and have a cutaway to prevent cells from being smeared as the chamber assembly is removed. Indexing pins on the frame ensure correct Cytopad alignment. Cytopads come pre-attached to chambers or in boxes of 100 for attaching to reused chambers.

  • Page 19: Barcode Reader

    SECTION 1 INTRODUCTION 1.1 Cytopro Cytocentrifuge Overview Barcode Reader An optional barcode reader is available for the Cytopro Cytocentrifuge. Figure 11: Barcode Reader...

  • Page 20: Touchscreen And User Interface

    SECTION 1 INTRODUCTION 1.2 Touchscreen and User Interface Users control all instrument functions from the interactive touchscreen display. Table 5: Front Panel/Main Screen Function Keys Button Name Description Standby/Ready With instrument power ON: Blue = Ready Amber = Standby Pressing Standby places instrument into standby mode The Standby/Ready button also accesses the touchscreen calibration function.
  • Page 21 SECTION 1 INTRODUCTION 1.2 Touchscreen and User Interface Table 5: Front Panel/Main Screen Function Keys (continued) Button Name Description Back Returns to the previous menu Stop Aborts any operation Indicates completion of current task System Setup Allows users to modify the software settings. See System Setup menu, (Section 2.1) Table 6: System Setup Keys Button...
  • Page 22 SECTION 1 INTRODUCTION 1.2 Touchscreen and User Interface Table 6: System Setup Keys (continued) Button Name Description Set Date/Time Allows users to set the date and time Restore Defaults Restore programming to default settings Unselected Shows an unselected option Selected Shows a selected or enabled option Login Enters Login sequence for authorized users.

  • Page 23: Instrument Setup

    SECTION 1 INTRODUCTION 1.3 Instrument Setup Unpacking and Installing the Instrument CAUTION: Contact Elitech Group before installing the instrument if you observe any damage to the packaging or equipment. Unpack and inspect the instrument. Check that the contents of the boxes match the packing lists for instrument and accessories.

  • Page 24: Controlling And Customizing Cytocentrifuge Functions

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Many software settings can be controlled from the System Setup menu. Accessing the System Setup Menu Press System Information. Press System Setup. Creating a Cytocentrifuge Program From the System Setup menu, press Cyto Programs. Press Add.

  • Page 25: Editing A Cytocentrifuge Program

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Editing a Cytocentrifuge Program From System Setup, press Cyto Programs. Select the program to be modified. Press Edit. Adjust the settings as desired. Press Save. Changing the Program Name: From Cyto Program Settings menu, select Program Name.

  • Page 26: Creating User Accounts

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Creating User Accounts Select System Setup. Enter the Administrator password. Press Users. Select Enable Global Login. Select Add User. Enter a user name. Press Enter. Enter a numeric passcode (at least 4 numbers) for the user account. Press Enter.

  • Page 27: User Login/Logout

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu User Login/Logout With System Access locked and Global Login enabled, users must log in to use the cytocentrifuge: Select User ID and select Logout Time After Idle For: from the drop-down menu.

  • Page 28: System Log

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu System Log The instrument records all login, logout, cytocentrifuge cycles, setting changes, and specimen identification (if enabled). Accessing Logs From System Setup, press System Log. Use navigation arrows to scroll through the log. Exporting Logs From System Setup, Press System Log.

  • Page 29: Slide And Specimen Tracking

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Slide and Specimen Tracking Under system default settings, the following options are disabled:  Enable Cyto Slide Tracking  Enable Manual Entry Enable Cyto Slide Tracking To activate Stain Slide Tracking, use the following steps: From System Setup, Press Tracking.

  • Page 30: Restoring Software Defaults

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.1 System Setup Menu Restoring Software Defaults From System Setup, select Restore Defaults. CAUTION: Restoring the system defaults will remove all personal settings.  Restoring System Settings will delete all user names and passwords as well as all stain and cytocentrifuge programs.

  • Page 31: Recording Specimen And Slide Information

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.2 Recording Specimen and Slide Information Scanning Slides with the Barcode Reader From System Setup select Tracking. Select Enable Cyto Slide Tracking. NOTE: Selecting Enable Cyto Slide Tracking changes the Start button on the Main menu to: “Load Slides.”...

  • Page 32: Manually Entering Specimen Information

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.2 Recording Specimen and Slide Information Manually Entering Specimen Information With Cyto Slide Tracking and Manual Entry enabled in the Tracking menu: Press Load Slides on the Main Menu. Press Add to reveal the keypad. Enter slide information (maximum of 24 characters) and/or specimen information (using the ID accompanying the specimen) and press Return.

  • Page 33: The Help Menu

    SECTION 2 CONTROLLING AND CUSTOMIZING CYTOCENTRIFUGE FUNCTIONS 2.3 The Help Menu The Help menu is a comprehensive onscreen help function that provides detailed information on the following subjects: Basic Operation  System Setup Help  Setting Up Cyto Programs  Setting Up Users ...

  • Page 34: Operating The Cytocentrifuge

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Suggested Cytocentrifugation Protocol  If enabled, scan or enter cyto slide and/or specimen information.  Prepare and load slides into the rotor.  Load chambers into the rotor.  Load samples into the chambers. ...

  • Page 35: Loading Chambers Into The Rotor

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Preparing and Loading Slides (continued) If Slide Tracking is enabled, press Load Slides.  If using the barcode reader (Section 2.2, Recording Specimen and Slide Information), scan each specimen slide and specimen barcode before loading it into the carousel.

  • Page 36: Loading Samples

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Loading Samples Load sample and prewetting fluids through cap vents, or directly into the ports before the cap is placed on the chamber. Use the chamber caps to minimize contamination and accidents. Chamber caps are mandatory for the Cytopro Magnum.

  • Page 37: Unloading The Rotor

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle The display shows the progress of the program, and a signal tone (if enabled) indicates the end of the cycle. NOTE: Use the emergency Stop button when required, for example, if there is abnormal vibration or noise.

  • Page 38: Separating Chambers From Cytopads

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Unloading the Rotor (continued) Cytopro Magnum chambers must remain in the rotor and in contact with the slide for 45 seconds after cytocentrifugation has stopped. This allows the fluid to be completely absorbed by the absorbent media.

  • Page 39: Attaching Cytopads To Chambers

    SECTION 3 OPERATING THE CYTOCENTRIFUGE 3.1 Running a Cytocentrifuge Cycle Attaching Cytopads to Chambers To reuse a chamber, attach a new Cytopad after the chamber is cleaned, disinfected, and thoroughly dried. To assemble Cytopads with sample chambers: Place a Cytopad inside a chamber frame, using the indexing pins for correct positioning.

  • Page 40: Preventive Maintenance And Safety

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.1 Routine and Preventive Maintenance The Cytopro is designed to be simple to use and care for with few user-serviceable components. Maintenance is primarily keeping the instrument and rotor clean (see Section 4.2). Other preventive measures are listed below: ...

  • Page 41: Replacing Fuses

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.1 Routine and Preventive Maintenance Replacing Fuses WARNING! To prevent the risk of fire, the main fuses should only be replaced with fuses of the same type and rating. Recurring fuse failure indicates serious internal problems, if this occurs, contact Elitech Group.

  • Page 42: Cleaning And Decontamination Procedures

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures The rotor and chambers are designed to reduce the risk of fluid escaping into the rotor interior during cytocentrifugation. However, the chambers do not fully eliminate biohazard contamination risks. Introducing samples into the chambers, insecurely placed caps, improper placement of chambers, and/or exceeding the maximum volume can lead to contamination of the rotor interior.

  • Page 43: Cleaning The Case Exterior And Lid

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures Cleaning the Case Exterior and Lid Cleaning these surfaces requires care to avoid damage. Never use abrasive powdered cleaners, or solvents as described previously. WARNING! Disconnect the instrument from line power before cleaning with liquids. Wipe surfaces with warm soapy water.

  • Page 44: Autoclaving The Rotor

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures Autoclaving the Rotor Autoclave the rotor for 60 minutes at 132 °C. Note: Open the lid to allow steam penetration inside the rotor. WARNING! All rotor seals can be sterilized with the rotor, either chemically or by autoclaving.

  • Page 45: Chemically Disinfecting Single Or Dual Chambers

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.2 Cleaning and Decontamination Procedures Chemically Disinfecting Single or Dual Chambers Remove used Cytopads as described in Section 3.1. Submerge chambers and frames in diluted (1/10) household bleach. Make dilution fresh each day. Soak for at least one hour. Scrub interior chamber surfaces with detergent-soaked cotton-tipped swab to remove residual cells.

  • Page 46: Shipping Or Disposing Of The Instrument Or Rotor

    SECTION 4 PREVENTIVE MAINTENANCE AND SAFETY 4.3 Shipping or Disposing of the Instrument and Rotor Shipping the Instrument or Rotor WARNING! You must disinfect the instrument or rotor before returning it to Elitech Group. The operating authority must complete a Hazard Free Certification Form (see below), otherwise the distributor or service center may not accept the instrument;...

  • Page 47: Solving Problems

    SECTION 5 SOLVING PROBLEMS 5.1 Troubleshooting This section helps you identify and solve routine problems with the Cytopro. More difficult problems may require technical service. Contact your Elitech Group representative for assistance. WARNING! Due to the electrical shock hazard, do not open this instrument or attempt internal repairs. Refer servicing to qualified service personnel.
  • Page 48 SECTION 5 SOLVING PROBLEMS 5.1 Troubleshooting Problem Solution Error messages on the screen. If the display shows Lid Not Shut: Verify that the lid is fully closed and latched. If the Lid Not Shut indication remains, contact Elitech Group for assistance. If the display shows Wrong Rotor after pressing Start: Make sure the rotor is properly loaded on the drive hub.

  • Page 49: Calibrating The Touchscreen

    SECTION 5 SOLVING PROBLEMS 5.2 Calibrating the Touchscreen Press and hold Standby/Ready for 5 seconds. A calibration screen with a target appears. Press the center of the target with a finger, stylus, or similar tool. Another target will appear in a different location. Continue to press the center of the targets until you have pressed all the targets (five total).

  • Page 50: Customer Service

    Elitech Group’s Service Department will help you resolve any questions about the operation or performance of your Cytopro Cytocentrifuge. Customers in the United States should contact us by telephone. Outside the U.S., our authorized dealers offer full local service and support. ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 USA Telephone: 800 453 2725 (United States &...

  • Page 51: Appendix A: Critical Reagent Components

    APPENDIX Critical Reagent Components The following information is to identify the critical chemicals of each reagent used in this instrument. SS-133 Decontamination Solution Concentrate contains: <30% Germicidal Detergent >70% Deionized Water SS-133 Decontamination Solution Concentrate is associated with the following Risk and Safety Phrases. European Symbol of Danger is Xi.

  • Page 52: Appendix B: Accessories And Supplies

    APPENDIX Accessories and Supplies Only replacement parts supplied by Elitech Group should be used with the Cyopro Cytocentrifuge. Use of non- approved parts may affect the performance and safety features of this product. ACCESSORIES REFERENCE NUMBER Cytopro Cytocentrifuge Rotor ....................AC-160 SUPPLIES REFERENCE NUMBER Cytopro Rotor Maintenance Kit ....................
  • Page 53   ELITechGroup   I nc.   370   W est   1 700   S outh   Logan,   U tah   8 4321-­‐8212   USA   800   4 53   2 725   +1   4 35   7 52   6 011  ...

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