ELITechGroup AEROSPRAY 7122 Applications Manual
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ELITechGroup AEROSPRAY 7122 Applications Manual

Hematology stat
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AEROSPRAY
HEMATOLOGY STAT
MODEL 7122
Applications
Manual
SLIDE STAINER/CYTOCENTRIFUGE

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Do you have a question about the AEROSPRAY 7122 and is the answer not in the manual?

Questions and answers

Danielle
March 3, 2025

Should the hub pattern be the color of each reagent - it doesn't specify in the manaul. Like C should be orange while A and D are clear and B is blue-purple.

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Summary of Contents for ELITechGroup AEROSPRAY 7122

  • Page 1 ® AEROSPRAY HEMATOLOGY STAT MODEL 7122 Applications Manual SLIDE STAINER/CYTOCENTRIFUGE...
  • Page 2 AEROSPRAY HEMATOLOGY STAT ® SLIDE STAINER/CYTOCENTRIFUGE Model 7122 Applications Manual 57-2000-02C Last Revision 01/08/2021...
  • Page 3 ©2021 ELITechGroup Inc. All rights reserved. Printed in the United States of America. No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language (human or computer) in any form, or by any means whatsoever, without the prior express written permission of ELITechGroup Inc.

  • Page 4: Table Of Contents

    Table of Contents Section 1 Introduction 1.1 Instrument Overview ..............7 Using this Manual ..............7 Functional Description ............8 Key Features ................. 9 Intended Use ................ 9 Table 1: General Specifications..........10 Table 2: Performance Specifications........11 Table 3: Run Time Sequence ..........12 Table 4: Approximate Reagent Consumption .......
  • Page 5 Table of Contents Section 3 Controlling and Customizing Stainer Functions (continued) Creating User Accounts ..........38 Managing User Access ........... 39 User Login/Logout ............39 Using Reagent Information Tracking ........41 Modifying Level Detect Functions ........41 Changing User Language ............42 Setting the Date and Time ............
  • Page 6 Table of Contents Section 5 Preventive Maintenance and Safety (continued) 5.2 Storing the Instrument ..............61 Preparing for Long-Term Storage ......... 61 5.3 Replacing Fuses ................62 5.4 Cleaning the Stainer and Carousels ..........63 Cleaning Liquid Spills ............63 Cleaning Broken Slides ............
  • Page 7 Table of Contents Section 8 Cytopro® Cytocentrifuge 8.1 Cytopro Cytocentrifuge Information ..........88 Functional Description ............88 Key Features ................. 88 Cytopro® Rotor ..............88 Appendix A: Critical Reagent Components ..........Appendix B: Risk and Safety Phrases ............Appendix C: Accessories and Supplies ............

  • Page 8: Instrument Overview

    SECTION 1 INTRODUCTION 1.1 Instrument Overview Using this Manual This manual provides instructions to install, operate, and maintain the Aerospray Hematology Stat Stainer/Cytocentrifuge. The manual is an important part of the product. Read it carefully and completely before setup and first use of the instrument. If additional accident prevention and environmental protection requirements exist in the country of operation, this manual must be supplemented by appropriate instructions to ensure compliance.

  • Page 9: Functional Description

    CAUTION: Only spare parts supplied or specified by ELITechGroup should be used in this device. Using non-approved parts may affect the performance and safety features of the instrument. If the equipment is used in a manner not specified by ELITechGroup the protection provided by the equipment may be impaired.

  • Page 10: Key Features

    SECTION 1 INTRODUCTION 1.1 Instrument Overview Key Features • Minimized reagent consumption Rapid staining • Barcode scanner for tracking specimens and reagents • • Reagent and specimen traceability • User traceability Administrator password • • Interactive touchscreen display • Multiple languages High-volume staining productivity (12 slides per stain cycle) •...
  • Page 11 SECTION 1 INTRODUCTION 1.1 Instrument Overview Table 1: General Specifications Category Characteristics Slide Carousel Capacity 1 to 12 Carousel Rotation Speed Reagent application: 20 rpm (± 5%) Slide Drying: 950 rpm (± 5%) Cytocentrifuge Rotor Speed 100 to 2000 rpm (± 5%), user programmable Reagent Consumption Refer to Approximate Reagent Consumption, Table 4 Operating Time...
  • Page 12 Reagent Spray Nozzles Four reagent nozzles: A, B, C, D Reagents: A - Rinse NOTE: Use only ELITechGroup reagents, with diluents B – Thiazin Stain (Blue) as specified for ELITechGroup concentrated reagents. REF numbers for this stainer start with one of the C –...
  • Page 13 SECTION 1 INTRODUCTION 1.1 Instrument Overview Table 3: Run Time Sequence NOTE: Table 3 represents a typical timing sequence for this instrument using the 12-slide carousel, presented for general reference only. Timing is in seconds. Actual cycle times may vary. Staining Mode Medium Dark Setting (5) Fixation Pump D (0ptional)
  • Page 14 SECTION 1 INTRODUCTION 1.1 Instrument Overview Table 5: Carousel and Rotor Information Only the following slide staining carousel or cytocentrifuge rotor can be used in this instrument. Each should be used following the instructions in this manual or the Cytopro Applications Manual (RP-517).
  • Page 15 SECTION 1 INTRODUCTION 1.1 Instrument Overview Table 6: Explanation of Symbols Alternating Current (AC) Authorized Representative in the European Community Batch Code Biological Hazards (Biological Risks) Catalog Number (Model Number) Caution, Consult Accompanying Documents (Attention, see instructions for use) CE Mark, product meets the essential requirements designated in Annex I of the In Vitro Device Directive (IVDD) 98/79/EC Consult Instructions For Use Do Not Reuse...
  • Page 16 “On” (Power) “Off” (Power) Environment Friendly Use Period Serial Number Temperature Limitation – indicates high and low limits. (Normal room temperatures are specified for all ELITechGroup Reagents.) Use By Volume Control General Warning, Caution, Risk of Danger Warning, Biological Hazard...

  • Page 17: Instrument Description

    SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 1: Front and Right Side Panels 1 – Front Panel with Touch Screen Display 2 – Bowl 3 – Carousel 4 – Lid with Safety Lock 5 – Right Side Panel with Label Indicating Reagent Positions: A –...
  • Page 18 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 2: Front Panel and Touchscreen 1 – Standby/Ready Button 2 – Touchscreen The front panel features an interactive touchscreen display. Refer to Front Panel/Main Screen Function Keys (Section 1.3, Table 8) for more information. Figure 3: Rear Panel 1 1 2 10 9 8...
  • Page 19 SECTION 1 INTRODUCTION 1.2 Instrument Description Figure 4: Stainer Bowl Components 2 3 4 1 – Nozzle D (Methanol or Aerofix) 2 – Nozzle C (Eosin stain) 3 – Nozzle B (Thiazin stain) 4 – Nozzle A (Rinse) 5 – Drive Hub Figure 5: Nozzle Diagram 1 –...
  • Page 20 SECTION 1 INTRODUCTION 1.2 Instrument Description Table 7: Preventive Maintenance Kit Component Name Description Manual Priming Tool Primes air-locked pumps. Silicon Grease Lubricates the nozzle threads for easy assembly. Nozzle Wire Cleans nozzle housing orifices. Nozzle Cleaning Strainer Strains the nozzle parts to prevent them from going down a drain.

  • Page 21: Barcode Reader

    SECTION 1 INTRODUCTION 1.2 Instrument Description Table 7: Preventive Maintenance Kit (continued) Component Name Description Volume Test Collection Collects reagents while performing Tubes (small tube) the Volume Test. Nozzle Maintenance Tube Holds Nozzle Cleaning Tubes (large Stand tube) and Volume Test Tubes (small tube).

  • Page 22: Touchscreen And User Interface

    SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Users control all instrument functions from the interactive touchscreen display. Table 8: Front Panel/Main Screen Function Keys Button Name Description Standby/Ready With instrument power ON: Blue = Ready Amber = Standby Pressing Standby runs a System Clean cycle and places instrument into standby mode.
  • Page 23 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 8: Front Panel/Main Screen Function Keys (continued) Button Name Description Back Returns to the previous menu. Stop Aborts any operation. Indicates completion of current task. System Setup Allows users to modify the software settings. See System Setup Menu (Section 3.1).
  • Page 24 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 9: System Setup Keys (continued) Button Name Description Set Date/Time Allows users to set the date and time. Restore Defaults Restores programming to default settings. Login Enters Login sequence for authorized users. Logout Logs authorized users out.
  • Page 25 SECTION 1 INTRODUCTION 1.3 Touchscreen and User Interface Table 10: Maintenance Function Keys Button Name Description Individual Prime Primes the selected line. Buttons (A, B, C, D) Pattern Test Performs Pattern Test to ensure nozzles are clear of debris and spraying properly. Volume Test Performs Volume Test to verify the selected nozzle volume is within the correct range.

  • Page 26: Setup And Preparation For Operation

    Follow this sequence if you are using this instrument for the first time. Details about these operations are given in the next three sections. CAUTION: Contact ELITechGroup before installing the instrument if you observe any damage to the packaging or equipment. Unpack and inspect the instrument.

  • Page 27: Connecting The Drain Tube And Waste Container

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Connecting the Drain Tube and Waste Container Insert the waste tube connector into the rear panel receptacle until you hear a click. Adjust the tube length to less than 1.8 m (72 in.). NOTE: Ensure the waste tube has no loops or kinks, and is as straight and as short as possible.

  • Page 28: Installing Reagent Bottles

    CAUTION: To avoid severe damage, never use reagents containing organic solvents (such as ketones) in this instrument, unless supplied by ELITechGroup, or specified in official ELITechGroup formulation instructions. NOTE: Immediately remove spills in the reagent tray to preserve the accuracy of the reagent level detecting system.

  • Page 29: Installing The Barcode Reader

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.1 Instrument Setup Installing the Barcode Reader A barcode reader can be connected to the stainer for scanning reagent bottles and specimen slides that contain barcodes. This allows easy reagent and specimen information tracking. If a barcode reader is not installed, reagent and specimen information can be entered manually (Section 3.2).

  • Page 30: Preparing The Stainer For Operation

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures NOTE: The instrument is shipped with alcohol in the reagent lines. For proper performance, this alcohol must be replaced with the correct reagent for each reagent line prior to use.
  • Page 31 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Priming Procedures (continued) Repeat the previous steps for each reagent line (B, C, and D). Press 60-Sec Prime to prime each reagent line with 200 mL of reagent to remove all of the alcohol from the reagent lines and pumps.

  • Page 32: The Clean Cycle

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation The Clean Cycle The Clean cycle is a two-step process that cleans the reagent nozzles and the carousel. The first step purges each reagent line and cleans the empty carousel with methanol or Aerofix while cycle progress is displayed on the screen.

  • Page 33: Reagent Level Monitoring

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Reagent Level Monitoring Reagent Level Detect monitors reagent levels and alerts you when the reagent is running low, or when the waste container is full (when using the waste container with level detect (AC-182)).

  • Page 34: Zeroing The Reagent Level Sensors

    SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Zeroing the Reagent Level Sensors The Level Detect function must be zeroed at initial setup, when the stainer is moved, or if the level detect is not reporting correctly. If zeroing does not correct the problem, recalibrate the Level Detect function (Section 7.3).
  • Page 35 SECTION 2 SETUP AND PREPARATION FOR OPERATION 2.2 Preparing the Stainer for Operation Zeroing the Reagent Level Sensors (continued) Remove all reagent bottles and press Start. NOTE: Vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. After zeroing, press OK.

  • Page 36: Controlling And Customizing Stainer Functions

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Many software settings can be controlled from the System Setup menu, including: Creating, editing, and deleting stain programs • • Creating, editing, and deleting cytocentrifuge programs • Tracking reagent information •...

  • Page 37: Creating A Stain Program

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Creating a Stain Program From System Setup, press Stain Programs. Press Add. Select Enter Program Name, and enter the desired name on the keypad. Press Enter on the keypad. The display returns to the Stain Programming Menu.

  • Page 38: Administrator And User Accounts

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Editing, Renaming, or Adjusting Stain Programs (continued) Adjust the settings as needed. Press Save. Administrator and User Accounts You can create one Administrator account and multiple (up to 50) user accounts. The Administrator controls access to the system by adding and editing user accounts.

  • Page 39: Creating User Accounts

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Creating User Accounts Note: This function is available only if an administrator account has been created. Press System Setup. Enter the Administrator password. Press Enter. Press Users to reveal the Manage Users menu. Select Enable Global Login.

  • Page 40: Managing User Access

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Creating User Accounts (continued) Enter a numeric passcode (at least 4 numbers) for the user account. 10 Press Enter. 11 Re-enter the passcode to confirm. 12 Press Enter. Managing User Access From the Manage Users screen, the Administrator has several options to manage user access to the instrument.
  • Page 41 SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu User LogIn/Logout (continued) Press Login. Enter the correct passcode for the selected user and press Enter. Once Login is complete, the display advances to the Main screen and the instrument is ready for programming and staining.

  • Page 42: Using Reagent Information Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Using Reagent Information Tracking You can enter reagent information to help track reagent usage and expiration. Reagent information includes reference number, expiration date, lot number, date and time the reagent was last installed.

  • Page 43: Changing User Language

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Changing User Language From System Setup, press Language. Select the software language from the list on the left. Press OK. Setting the Date and Time From System Setup, press Set Date/Time. Select 12 for a 12-hour clock or 24 for a 24-hour clock.

  • Page 44: Exporting Logs

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu System Log (continued) Exporting Logs From System Setup, press System Log. Plug a Flash Drive into the right USB port. Press Export. NOTE: The log files are exported to the Flash Drive as a CSV file that can be used in spreadsheet software programs.

  • Page 45: Qc/Maintenance Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu QC/Maintenance Tracking Under system default settings, the following QC/Maintenance Tracking options are disabled: Stain Slide Tracking • • Cyto Slide Tracking • Manual Entry • Preventive Maintenance Tracking • Reagent Tracking Enable Stain Slide Tracking To activate Stain Slide Tracking:...

  • Page 46: Enable Cyto Slide Tracking

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Enable Cyto Slide Tracking Allows slide tracking in cytocentrifuge mode. See the Cytopro Rotor Applications Manual (Aerospray Models 7xx2) (REF: RP-517) for complete information. Enable Manual Entry If selected, allows manual entry of slide information using the keypad (limited to 24 characters).

  • Page 47: Restoring Software Defaults

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.1 System Setup Menu Restoring Software Defaults From System Setup, select Restore Defaults. CAUTION: Restoring the system defaults will remove all personal settings. • Restoring System Settings will delete all user names and passwords. •...

  • Page 48: Recording Specimen And Reagent Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Slides with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Stain Slide Tracking. NOTE: Selecting Enable Stain Slide Tracking changes the Start button on the Main menu to ‟Load Slides.”...

  • Page 49: Scanning Reagent Bottles With The Barcode Reader

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Scanning Reagent Bottles with the Barcode Reader From System Setup select QC/Maintenance Tracking. Select Enable Reagent Tracking for each desired reagent (A, B, C, D). Press Back to return to the System Setup menu. Press Reagents to reveal the Reagent Information screen.

  • Page 50: Manually Entering Specimen Information

    To change or delete the entry, select the entry on the display and press Edit or Remove. Load slides and run stain cycle as shown in Section 4.1. NOTE: Reagent REF number must be a valid ELITechGroup REF number for the selected reagent. Incorrect entries will generate an error message.

  • Page 51: Manually Entering Reagent Information

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.2 Recording Specimen and Reagent Information Manually Entering Reagent Information Press Reagents from the System Setup menu, or press the reagent status icon on the Main menu to reveal the Reagent Information menu. Select the desired reagent and press Change.

  • Page 52: The Help Menu

    SECTION 3 CONTROLLING AND CUSTOMIZING STAINER FUNCTIONS 3.3 The Help Menu The Help menu is a comprehensive onscreen help function that provides detailed information on the following subjects: Help Screens Basic Operation • Loading the Carousel • Programming Number of Slides Select Intensity Setting •...

  • Page 53: Operating The Stainer

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions WARNING! Treat slides in accordance with good laboratory practices and local regulations. Suggested Staining Protocol • Hub Pattern Test (once per day). • Select or verify desired stain program. • If slide tracking is enabled, scan or enter slide information. •...

  • Page 54: Loading The Carousel

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Loading the Carousel CAUTION: Never load chipped or cracked slides into the instrument. Slides in poor condition may break during the staining cycle. If a slide breaks in the bowl, see Cleaning Broken Slides (Section 5.4).
  • Page 55 SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Loading the Carousel (continued) Load slides with the labels toward the outside edge of the carousel. • Always load slides with the specimen facing clockwise. • Always place the first slide in position 1, the second in position 2, and •...

  • Page 56: Using Blocking Slides

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Using Blocking Slides If the carousel is not full, blank slides should be used as blocking slides. Blocking slides prevent overspray of reagents onto the specimen slides. Overspray can cause slides to become over-stained.

  • Page 57: Unloading The Carousel

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Performing a Stain Cycle (continued) If you have created a stain program, and it appears on the display, proceed to Step 4. If the desired program does not appear on the display, press Programs.

  • Page 58: Monitoring Reagent And Waste Levels

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions Monitoring Reagent and Waste Levels If enabled, the stainer displays the approximate reagent and waste container levels and other information. CAUTION: You must monitor the reagent and waste container levels on the display (if enabled) and by direct inspection of the bottles.

  • Page 59: Replacing A Reagent Bottle

    SECTION 4 OPERATING THE STAINER 4.1 Operating Instructions NOTE: Access the Reagent Information menu by pressing the bottle icons on the right side of the Main menu. Press Change to scan or manually enter reagent information. NOTE: Do not put residual reagent from a used bottle into a new bottle. This can lead to an accumulation of residue on the slides and may be a source of contamination.

  • Page 60: Preventive Maintenance And Safety

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance The system provides a Preventive Maintenance Log for tracking the most recent maintenance activities. See Enable Preventive Maintenance Tracking (Section 3.1) and Using the Preventive Maintenance Log in this section. Daily Maintenance/Quality Control (QC) Check reagent levels and expiration dates.

  • Page 61: Monthly Maintenance

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.1 Preventive Maintenance Monthly Maintenance Disassemble and manually clean all nozzles. Refer to Nozzle Disassembly and Cleaning (Section 6.1). Disinfect any reagent bottle that is being reused (Section 5.5). Ensure the maintenance procedures in the Preventive Maintenance (PM) Log have been performed, and entered into the PM chart or log.

  • Page 62: Storing The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.2 Storing the Instrument If the instrument is inactive for more than one week, you may want to perform the long-term storage procedure. This will prevent nozzles from clogging when the machine is reactivated. Preparing for Long-Term Storage With the carousel removed, remove and clean the nozzles.

  • Page 63: Replacing Fuses

    To prevent the risk of fire, the main fuses should only be replaced with fuses of the same type and rating. Recurring fuse failure indicates serious internal problems, if this occurs, contact ELITechGroup. Power OFF the instrument. Disconnect the power cord from the power outlet and the rear panel of the instrument.

  • Page 64: Cleaning The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.4 Cleaning the Stainer and Carousels WARNING! All cleaning procedures should be performed in a well-ventilated room by authorized and trained personnel wearing appropriate protection equipment. Clean the outside of the instrument with 70 to 100% ethanol or methanol. Clean the carousel and lid with 70 to 100% ethanol or methanol.

  • Page 65: Decontaminating The Stainer And Carousels

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels All parts of the instrument that come into contact with biological specimens, patient specimens, positive control specimens, or hazardous material must be treated as potentially infectious. Before the instrument is returned for service, all outer surfaces must be decontaminated. The operating authority must complete a disinfection declaration, otherwise the instrument may be rejected by the distributor or service center or quarantined by customs authorities.

  • Page 66: Decontaminating The Instrument

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Figure 12: Lid Latch and Locking Pin Hole Locations 1 – Lid Latch Hole 2 – Locking Pin Hole Decontaminating the Instrument Prepare a suitable container for all disposables. Cover the lid latch and locking-pin holes with waterproof tape to protect the interior (Figure 12).

  • Page 67: Decontaminating Reagent Bottles

    SECTION 5 PREVENTIVE MAINTENANCE AND SAFETY 5.5 Decontaminating the Stainer and Carousels Decontaminating the Instrument (continued) Repeat the spray treatment of exterior surfaces every 2 or 3 minutes for a total of 20 minutes. Do not allow cleaning/decontamination solutions to dry on the instrument surfaces.

  • Page 68: Shipping Or Disposing Of The Stainer Or Carousels

    Shipping the Instrument or Carousels WARNING! You must disinfect the instrument or carousels before returning it to ELITechGroup. The operating authority must complete a Hazard-Free Certification form, otherwise the distributor or service center may not accept the instrument; or customs authorities may hold it.

  • Page 69: Nozzle Maintenance And Performance

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.1 Nozzle Disassembly and Cleaning Nozzle maintenance requires the Nozzle Maintenance Kit and prepared Nozzle Cleaning Solution (diluted REF: SS-029C). WARNING! Always wear protective clothing and eye protection when using Nozzle Cleaning Solution (diluted REF: SS-029C). Dispose of used solution properly. NOTE: If the compression screw cannot be easily loosened, use light penetrating oil and a 5/8-in.

  • Page 70: Nozzle Reassembly

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.2 Nozzle Reassembly Hold your thumb or a nozzle strainer over the end of the tube to keep the nozzle parts in the tube. Discard the cleaning solution according to applicable statutes. Swirl Cone Inspect the nozzle parts.

  • Page 71: Manual Priming

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.3 Manual Priming Remove the carousel from the bowl. Remove the nozzle connected to the line to be manually primed. Insert the priming tool nozzle adapter (included in the Nozzle Maintenance Kit) into the nozzle holder and turn it clockwise to install the adapter into the holder.

  • Page 72: Performing The Volume Test

    Clear the nozzle orifice with the nozzle brush found in the maintenance kit. b. If necessary, remove the nozzle and perform the Nozzle Cleaning procedure (Section 6.1). If the problem persists, replace the nozzle. NOTE: If the problem persists after you have replaced the nozzle, contact ELITechGroup.
  • Page 73 SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.4 Performing the Volume Test Prepare the Maintenance Kit for future use: • Empty the contents of the tubes into the stainer bowl. Rinse the tubes with water. • Put the tubes back into their original place in the Maintenance Kit or •...

  • Page 74: Performing The Slide Pattern Test

    SECTION 6 NOZZLE MAINTENANCE AND PERFORMANCE 6.5 Performing the Slide Pattern Test This test can differentiate poor staining results from sample preparation problems, or nozzle obstructions. Perform the Slide Pattern test when a Hub Pattern test produces a normal result, but staining is still inadequate.

  • Page 75: Solving Problems

    Contact your ELITechGroup representative for assistance. WARNING! Due to the electrical shock hazard, do not open this instrument or attempt internal repairs. Refer servicing to qualified service personnel. Contact your dealer or ELITechGroup Service. Table 13: General Troubleshooting and Diagnosis Problem Solution There is no power to the stainer when the power switch Check the facility outlet and the power cord connection.
  • Page 76 Reagent leaks may indicate an internal problem (see Section 7.3). See the Aerospray Service Manual, or contact your dealer or ELITechGroup for further assistance. Error messages on the screen. If the display shows Lid Not Shut: Verify that the lid is fully closed and latched.
  • Page 77 To determine this, press Stop. If the cycle stops: this indicates a problem with the carousel position sensor. Consult the Aerospray Service Manual, or contact your dealer or ELITechGroup for assistance. If the cycle continues: this indicates an electronic problem (see below).
  • Page 78 SECTION 7 SOLVING PROBLEMS 7.1 Troubleshooting Table 13: General Troubleshooting and Diagnosis (continued) Problem Solution Abnormal staining on entire surface of all slides When staining a full carousel (7 or more slides) make (continued). certain you have not programmed the stainer for fewer slides.
  • Page 79 Make certain the blood smears are completely dry and not too thick (see Section 7.2). Increase the fixation setting to High. Call ELITechGroup for information on slide quality. Refractile artifacts are observed in erythrocytes. Use Aerofix (SS-048 or SS-148) fixative. Refer to Section 7.2.
  • Page 80 Do this by running the stainer while watching the display and listening to the pumps. Ensure that each event occurs according to the operating sequence, shown in Table 3 in Section 1. If the problem recurs, contact your dealer or ELITechGroup for assistance.

  • Page 81: Abnormal Staining Results

    SECTION 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Smear Separation or Tearing Losing blood cells from the slide surface during a staining cycle can happen for several reasons: Improper Alcohol Fixation If the alcohol-fix phase of the cycle fails, there will be cell loss when stain is applied.

  • Page 82: Use Aerofix

    SECTION 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Red Cell Artifacts (continued) When the relative humidity is above 60 percent, water spotting may be seen even when using anhydrous methanol for fixation. This occurs because of moisture accumulation and condensation inside the carousel chamber. Relative humidity within the chamber will tend to build up with repeated use, leading to further problems.
  • Page 83 SECTION 7 SOLVING PROBLEMS 7.2 Abnormal Staining Results Procedure: NOTE: Do not remove the nozzles. Pump 150 mL of prepared solution through each affected reagent line (A, B and C). Wait 20 minutes. Pump another 100 mL of disinfectant solution through each affected reagent line and let sit for 20 minutes.

  • Page 84: Instrument Malfunction

    A reagent line leak between the pump outlet and the nozzle will cause fluid to leak into the interior of the stainer housing and ultimately onto the counter. If you observe this, the instrument will require service. Contact your dealer or ELITechGroup for assistance. WARNING! A break or malfunction in the reagent delivery system can potentially release up to 500 mL of highly flammable anhydrous alcohol in and around the instrument.

  • Page 85: Reagent Level Detect System Errors

    SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Reagent Level Detect System Errors Reagent A-D Not Calibrated During the second part of calibration, if no bottles are detected, the display shows an error message. Calibrate again, making sure that the reagent bottles are inserted in those tray positions that have been enabled in the level detection system.
  • Page 86 SECTION 7 SOLVING PROBLEMS 7.3 Instrument Malfunction Calibrating the Reagent Level Detect System (continued) Remove all reagent bottles and press Start. NOTE: Any vibrations or bumps to the instrument or lab bench can cause inaccuracies in zeroing or calibration. NOTE: Calibration requires full, unopened (caps and seals in place) 500 mL bottles of reagent, placed in the correct tray positions (due to different densities of each reagent type).

  • Page 87: Calibrating The Touchscreen

    SECTION 7 SOLVING PROBLEMS 7.4 Calibrating the Touchscreen Press and hold Standby/Ready for 5 seconds. A calibration screen with a target appears. Press the center of the target with a finger, stylus, or similar tool. Another target will appear in a different location. Continue to press the center of the targets until you have pressed all the targets (five total).

  • Page 88: Service Information

    SECTION 7 SOLVING PROBLEMS 7.5 Service Information ELITechGroup’s Service Department will help you resolve any questions about the operation or performance of your Aerospray Stainer/Cytocentrifuge. Customers in the United States should contact us by telephone. Outside the U.S., our authorized dealers offer full local service and support.

  • Page 89: Cytopro® Cytocentrifuge

    SECTION 8 CYTOPRO® CYTOCENTRIFUGE 8.1 Cytopro Cytocentrifuge Information Functional Description The Cytopro Cytocentrifuge rotor allows rapid sedimentation of specimen cells onto microscope slides for staining or other purposes. Up to eight disposable/reusable sample chamber assemblies with absorbent pads and glass microscope slides can be loaded into the Cytocentrifuge rotor.

  • Page 90: Appendix A: Critical Reagent Components

    APPENDIX A Critical Reagent Components The following information identifies the critical chemicals of each reagent used in this instrument. Reagent(s) Critical Components SS-035A, SS-035A-EU, SS-035AG, or SS-135/149A (as <1% Potassium Phosphate diluted) Hematology Reagent A Rinse contains: <1% Sodium Phosphate <1% Eosin <1% Benzyl Alcohol SS-035/049B, SS-035/049B-EU, SS-035/049BG, or SS-...
  • Page 91 APPENDIX A Critical Reagent Components Reagent(s) Critical Components SS-135/149C, Reagent C Eosin Stain Concentrate <10% Potassium Phosphate contains: <10% Sodium Phosphate <0.2% Formaldehyde <3% Eosin SS-135S Surfactant contains: <70% Germall II (Diazolidinyl Urea) <5% Tergitol TMN 100X SS-148 or SS-148-EU, Aerofix Fixative Concentrate 55-70% Ethylene Glycol High Humidity contains: 30-45% Polyvinylpyrrolidone (PVP)

  • Page 92: Appendix B: Risk And Safety Phrases

    APPENDIX B Risk and Safety Phrases Reagents SS-035A, SS-035A-EU, and SS-035AG Reagent – A Rinse, and SS-135/149A – Rinse Reagent concentrate after dilution as directed, are associated with no Hazard and Precautionary statements. There is no Signal word. H Statements None P Statements None...
  • Page 93 APPENDIX B Risk and Safety Phrases Reagents SS-049C and SS-049C-EU – Basofix Eosin Stain, or SS-135/149C – Eosin Stain concentrate after dilution as directed, are associated with the following Hazard and Precautionary statement. The associated Signal word is Warning. H302 Harmful if swallowed H371 May cause damage to organs ( if swallowed)
  • Page 94 APPENDIX B Risk and Safety Phrases Reagents SS-148, SS-148-EU – Aerofix Additive for Methanol is associated with the following Hazard and Precautionary statements. The associated signal word is Warning. H302 Harmful if swallowed P264 Wash hands thoroughly after handling P270 Do not eat, drink or smoke when using this product P301+312 IF SWALLOWED: Call a POISON CENTER, a doctor if you feel unwell...
  • Page 95 APPENDIX B Hazard and Precautionary Statements SS-029C and SS-029C-EU Aerospray Nozzle Cleaning Solution is associated with the following Hazard and Precautionary statements. The associated Signal word is: Warning. H319 Causes serious eye irritation H314 Causes severe burns and eye damage P280 Wear protective gloves, protective clothing, eye protection P303+P361+P353...

  • Page 96: Appendix C Accessories And Supplies

    APPENDIX C Accessories and Supplies Only replacement parts supplied by ELITechGroup should be used in this instrument. Use of non-approved parts may affect the performance and safety features of this product. ACCESSORIES REFERENCE NUMBER Slide Carousel (12-Slide Capacity) ............AC-188 Cytopro Cytocentrifuge Rotor ..............AC-160 STAINS AND CLEANING REAGENTS Nozzle Cleaning Solution, 355 mL ............SS-029...
  • Page 97 ELITechGroup Inc. 370 West 1700 South Logan, Utah 84321-8212 800 453 2725 +1 435 752 6011 WWW.ELITECHGROUP.COM...

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