Contents Introduction ........................ 5 About this guide ....................6 Intended use of the QIAcuity ................7 Requirements for QIAcuity users ................. 8 Safety Information to Prevent Misuse and Personal and Material Damage ......9 Proper use ..................... 10 Electrical safety ....................12 Environment ....................
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Regular maintenance procedure for QIAcuity Software Suite ....... 63 Troubleshooting ......................65 General information..................65 Contacting QIAGEN Technical Services ............65 Performing a self-check on the QIAcuity instrument ..........66 Creating a support package with the QIAcuity instrument software ....... 67 Creating a support package with the QIAcuity Software Suite ......
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Liability Clause ....................90 Declaration list of China RoHS (SJT 11364-2014) ..........91 Document Revision History ..................94 QIAcuity Safety and Installation Guide 04/2021...
® Technical Services Departments are staffed by experienced scientists with extensive practical and theoretical expertise in molecular biology and the use of QIAGEN products. If you have any questions or experience any difficulties regarding the QIAcuity or QIAGEN products in general, do not hesitate to contact us.
About this guide This safety and installation guide provides information about QIAcuity in the following sections: Intended use Safety Information General description QIAcuity Installation QIAcuity Software Suite Installation Connecting the QIAcuity Software Suite to the QIAcuity instrument ...
QIAcuity systems such as QIAcuity Nanoplates and QIAcuity PCR Reagents for the applications described in the kit handbooks. If the QIAcuity is used with products other than QIAGEN kits or QIAGEN assays designed for dPCR, it is the user’s responsibility to validate the performance of such product combination for any particular application.
Laboratory technicians or equivalent experienced personnel familiar with use of computers and automation in general Trained and authorized by Preventive maintenance QIAGEN service personnel or QIAGEN service technicians of an authorized agent Trained and authorized by Servicing QIAGEN service personnel or...
Safety Information to Prevent Misuse and Personal and Material Damage Before using the QIAcuity, it is essential that you read this user manual carefully and pay attention to the safety information. The instructions and safety information in the user manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition.
The QIAcuity must only be operated by qualified personnel who have been appropriately trained. Servicing of the QIAcuity must only be performed by a QIAGEN Field Service specialist. Perform the maintenance as described in the Maintenance Procedures section. QIAGEN charges for repairs that are required due to incorrect maintenance.
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Risk of explosion [W7] The QIAcuity is intended for use with reagents and substances supplied with QIAGEN kits or other than outlined in respective Information for Use. Use of other reagents and substances may lead to fire or explosion. CAUTION...
Electrical safety Note: Disconnect the line power cord from the power outlet before servicing. WARNING Electrical hazard [W8] Any interruption of the protective conductor (earth/ground lead) inside or outside the instrument or disconnection of the protective conductor terminal is likely to make the instrument dangerous. Intentional interruption is prohibited.
If liquid has spilled inside the instrument, power OFF the instrument, disconnect it from the power outlet, and contact QIAGEN Technical Services. If the instrument becomes electrically unsafe, prevent other personnel from operating it, and contact QIAGEN Technical Services.
CAUTION Risk of overheating [C7] To ensure proper ventilation, maintain a minimum clearance of 10 cm at the sides and rear of the QIAcuity. Slits and openings that ensure the ventilation of the QIAcuity must not be covered. Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious.
WARNING Samples containing infectious agents [W12] Some samples used with this instrument may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations. Always wear safety glasses, 2 pairs of gloves, and a lab coat. The responsible body (e.g., laboratory manager) must take the necessary precautions to ensure that the surrounding workplace is safe, and that the instrument operators are suitably trained and not exposed to...
Maintenance safety WARNING/ Risk of personal injury and material damage [W14] CAUTION Only perform maintenance that is specifically described in this user manual. Risk of fire [W15] WARNING Do not allow cleaning fluid or decontamination agents to come into contact with the electrical parts of the QIAcuity. CAUTION Damage to the instrument [C8]...
Symbols on the QIAcuity Symbol Location Description Type plate on the back of the CE mark for European Conformity instrument Type plate at the back of the CSA listing mark for Canada and instrument the USA Type plate on the back of the RCM mark for Australia and New instrument Zealand...
PCR thermocycler, and a fluorescence imaging module. For more information refer to the QIAcuity user manual on www.qiagen.com. Figure 1. QIAcuity Four. QIAcuity Safety and Installation Guide 04/2021...
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QIAGEN offers different instrument configurations. QIAcuity One as 2-plex and 5-plex version. QIAcuity Four and QIAcuity Eight always offer 5-plex analysis. This table shows the available channels provided in each instrument. Table 2. QIAcuity instrument configurations Instrument Available channels Green...
QIAcuity Installation The installation procedure of the product is recommended to be carried out by a certified QIAGEN Field Service Specialist. A person who is familiar with the laboratory and computer equipment should be present during the installation. Installation environment...
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Note: We recommend to plug the instrument directly into its own power outlet and not to share the power outlet with another lab equipment. Do not place the QIAcuity on a vibrating surface or near vibrating objects. WARNING Explosive atmosphere (W8) The QIAcuity is not designed for use in an explosive atmosphere.
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Power requirements The QIAcuity operates at 100–240 V AC, 50/60 Hz, 1500 VA (max.) Ensure that the voltage rating of the QIAcuity is compatible with the AC voltage available at the installation site. Main supply voltage fluctuations should not exceed 10% of nominal supply voltages.
Grounding requirements To protect operating personnel, the National Electrical Manufacturers’ Association (NEMA) recommends that the QIAcuity be correctly grounded (earthed). The instrument is equipped with a 3-conductor AC power cord that, when connected to an appropriate AC power outlet, grounds (earths) the instrument. To preserve this protection feature, do not operate the instrument from an AC power outlet that has no ground (earth) connection.
Installing the QIAcuity This section describes important actions that must be performed before operating the QIAcuity. These actions include: Removal of the protective film from the QIAcuity hood Removal of the shipping fixation screw Connection of the power cord to the back of the QIAcuity ...
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Connecting the power cord to the back of the QIAcuity 1. Remove the power cord from the foam packing material on top of the QIAcuity. Note: Only use the power cord that is supplied with the QIAcuity. 2. Check that the voltage rating on the label at the back of the QIAcuity matches the voltage available at the installation site.
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If there is an initialization error, retry the initialization process by turning the instrument off and on again. If the problem persists, see Troubleshooting the instrument and software or contact QIAGEN Technical Services. Note: The instrument must be turned off at least once a week.
USB port, 1Gbps cable Ethernet Interface The QIAcuitySuite.msi package needed for the installation of the QIAcuity Software Suite is provided on the USB drive that comes with the QIAcuity. Alternatively, you can download the QIAcuitySuite.msi package from www.qiagen.com. QIAcuity Safety and Installation Guide 04/2021...
Installing the QIAcuity Software Suite To install the QIAcuity Software Suite on the notebook, follow these steps: 1. Before you begin the installation process using the QIAcuity Setup Wizard, turn off the standby/hibernation settings in the Windows configuration: Right-click the Windows start menu and select Power Options. Click change plan settings next to your current power plan.
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3. Check the I accept the terms in the License Agreement box in the End-User License Agreement window and click Next. QIAcuity Safety and Installation Guide 04/2021...
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4. Click Next in Destination Folder window. QIAcuity Safety and Installation Guide 04/2021...
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5. Click Install to start the installation. 6. In the prompt window asking you if you allow changes to your device, click Yes. QIAcuity Safety and Installation Guide 04/2021...
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Note: During installation, some command line windows may appear in the foreground. Do not close any of them. QIAcuity Safety and Installation Guide 04/2021...
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7. When the installation is completed, click Finish. 8. In the local host site that says the site is not secure, click Details. QIAcuity Safety and Installation Guide 04/2021...
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9. Click Go on to the webpage. You are directed to the QIAcuity Software Suite login screen. QIAcuity Safety and Installation Guide 04/2021...
For more information on upgrading or uninstalling the QIAcuity Software Suite refer to the QIAcuity user manual on www.qiagen.com Establish a connection between the QIAcuity instrument and the QIAcuity Software Suite The QIAcuity instrument needs to be connected to the QIAcuity Software Suite to enable the exchange of data.
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Figure 2. The QIAcuity instrument and QIAcuity Software Suite are connected via Ethernet cable. Alternatively, both the QIAcuity instrument and the computer running the QIAcuity Software Suite can be connected to a LAN (local area network). This configuration allows the QIAcuity notebook or a separate computer to work as a server to which multiple clients can be connected.
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10Mbit/s for a setup via network Note: Please make sure that the QIAcuity instrument is connected to the LAN. Any other configurations are not supported by QIAGEN. If you are unsure about your network infrastructure, please consult with your local IT.
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11. Click Network and Internet (if not available proceed to the next step). 12. Click Network and Sharing Center. 13. On the left pane, click Change adapter settings. 14. Right-click the ethernet network adapter and select Properties. 15. Select Internet Protocol Version 4 (TCP/IPv4) and click Properties. QIAcuity Safety and Installation Guide 04/2021...
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16. Select Obtain an IP address automatically. If your organization does not provide DNS details, select Obtain DNS server address automatically as well. Click OK, then Close. QIAcuity Safety and Installation Guide 04/2021...
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Optional: You can check if the addresses have been assigned properly by following the steps below. 1. From your Home screen, click the Search icon. Enter cmd and press the Enter key. 2. Wait for the command line window to open. Enter ipconfig command. Address should be visible under the ethernet interface name for which modifications were made QIAcuity Safety and Installation Guide 04/2021...
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Follow the steps below to configure the QIAcuity instrument. 1. On the toolbar, tap Configuration. 2. Select the Ethernet tab. QIAcuity Safety and Installation Guide 04/2021...
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3. Check the DHCP enabled box. If you check this box, the IP address and MAC address fields are disabled. The assigned IP and MAC addresses of device are displayed in the IP address and MAC address fields. 4. Tap Save. QIAcuity Safety and Installation Guide 04/2021...
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Configuring a direct cable connection between the QIAcuity instrument and the QIAcuity Software Suite Note: Before you start, ensure that the QIAcuity instrument and the notebook are connected with an Ethernet cable. Follow the steps below to configure the notebook running the QIAcuity Software Suite. 1.
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2. Click Network and Internet (if not available proceed to the next step). 3. Click Network and Sharing Center. 4. On the left pane, click Change adapter settings. 5. Right-click the ethernet network adapter and select the Properties option. 6. Select Internet Protocol Version 4 (TCP/IPv4) and click Properties: QIAcuity Safety and Installation Guide 04/2021...
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7. Select Use the following IP address. Enter the following information: IP address: Enter 192.168.1.1. Subnet mask: Enter 255.255.255.0. Default gateway: Enter 192.168.1.254. Preferred DNS server: Enter the DNS server address. Alternative DNS server: Enter the alternative DNS server address. ...
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Optional: You can check if the addresses have been assigned properly by following the steps below. 9. From your Home screen, click the Search icon. Enter cmd and press the Enter key. 10. Wait for the command line window to open. Enter ipconfig. Address should be visible under the ethernet interface name for which modifications were made.
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Follow the steps below to configure the QIAcuity instrument. 1. On the toolbar, tap Configuration. 2. Select the Ethernet tab. 3. Ensure that the DHCP enabled box is not checked. Enter the following information: IP address: Enter 192.168.1.2. Subnet mask: Enter 255.255.255.0. ...
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4. Tap Save. Configuring the connection to the QIAcuity Software Suite in the QIAcuity instrument software The QIAcuity instrument needs to be connected to the QIAcuity Software Suite to enable the exchange of data. To establish a connection, the instrument and the device in which the QIAcuity Software Suite is running must be connected to the same network.
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2. On the Home screen, tap Configuration. 3. Tap the Software Suite tab. QIAcuity Safety and Installation Guide 04/2021...
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4. Enter the URL in the Software Suite URL field. The Suite URL has the following format: https://<IP address of Suite Server>:8687/instrument/v0/ Note: To get the IP address of the Suite Server, the instrument must be connected to the Suite Server. From your Home screen, click the Windows icon and navigate to Command Prompt, or enter cmd in the Search field.
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Note: An error message displays if the URL you entered is invalid. Tap OK to close the error message, then re-enter the correct URL in the Software Suite URL field. QIAcuity Safety and Installation Guide 04/2021...
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5. If you want to configure the proxy settings in your network configuration, clear the Do not use a proxy box and enter the following information: Proxy Server: Enter the proxy server address. Proxy Port: Enter the port address. ...
Maintenance Procedures WARNING/ Risk of personal injury and material damage [W1] Only perform maintenance that is specifically described in this user CAUTION manual. The following maintenance procedures must be carried out to ensure reliable operation of the QIAcuity: Regular maintenance ...
Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Cleaning agents The following disinfectants and detergents are recommended for cleaning the QIAcuity. Note: If you want to use disinfectants different from those recommended, ensure that their compositions are similar to those described below.
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Removal of RNase contamination RNaseZap RNase Decontamination Solution (Ambion, Inc., cat. no AM9780) can be used ® for cleaning surfaces. RNaseZap can also be used to perform decontamination by spraying the respective items. Removal of nucleic acid contamination DNA-ExitusPlus™ (AppliChem, cat. no. A7089,0100) can be used for cleaning surfaces. DNA-ExitusPlus can also be used to perform decontamination by spraying the respective items.
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CAUTION Damage to the instrument [C10] Do not use bleach, solvents, or reagents containing acids, alkalis, or abrasives to clean QIAcuity. CAUTION Damage to the instrument [C11] Do not use spray bottles containing alcohol or disinfectant to clean surfaces of the QIAcuity. Take special care while cleaning the extended drawer that no liquid is spilled into the inside of the instrument.
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Improper use of the QIAcuity may cause personal injuries or damage to the instrument. The QIAcuity must only be operated by qualified personnel who have been appropriately trained. Servicing of the QIAcuity must only be performed by a QIAGEN Field Service specialist. WARNING...
Servicing Contact QIAGEN Technical Services or your local distributor for more information about flexible Service Support Agreements from QIAGEN. WARNING Risk of personal injury and material damage [W1] Improper use of the QIAcuity may cause personal injuries or damage to the instrument.
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1. Turn off instrument and remove power cord. 2. Reach under the front of the instrument and push both buttons simultaneously. 3. Remove the filter from the swing-out filter compartment. QIAcuity Safety and Installation Guide 04/2021...
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4. Replace with a new filter and push the compartment to the top to close. Calibration of thermal cycler The thermal cycler is designed to operate with the same specifications over the lifetime of the instrument. To ensure and verify the quality of the cycler, the calibration of the thermal cycler is part of an annual scheduled service visit.
If damaged plates were used and the inside of the instrument is contaminated, please contact QIAGEN Technical Services. The QIAcuity should also be decontaminated before shipping (e.g., back to QIAGEN). In this case, a decontamination certificate must be completed to confirm that the decontamination procedure has been carried out.
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Note: Only administrators can delete data from the instrument. 1. Tap the Tools icon. 2. Tap Data Management. 3. To clear the data, tap Force clear images. Click OK in the confirmation dialog box to delete the data. Images from the system and database will be removed. QIAcuity Safety and Installation Guide 04/2021...
Regular maintenance procedure for QIAcuity Software Suite To monitor the space of your disk, click Disk monitor in the main toolbar. This shows an overview about the state of the disk, disk name, and disk path. It also shows the remaining free space and total space of your disk.
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Table 4. Disk status State Meaning Flag Normal No threshold has been reached None Warning Disk space reached the warning Yellow dot in disk monitor icon level, there is only disk space for a few runs left Critical No disk space left to store more run Red dot in disk monitor icon data Unavailable...
Serial number of the instrument This information will help you and your QIAGEN Technical Service Specialist to deal most efficiently with your issue. Note: For most cases, to allow proper analysis of an error situation, the support package either from the instrument and/or the suite is required.
Performing a self-check on the QIAcuity instrument The QIAcuity software can perform a self-check of the instrument to check the state of the device. There are two types of self-checks: Quick test: This test does not include any hardware movement ...
Creating a support package with the QIAcuity instrument software You can create a support package in case of an error. The support package can be uploaded to the Software Suite or saved to a USB drive. To create a support package and upload it to the Software Suite, follow the steps below. The QIAcuity Software Suite will combine the support package from the instrument and the Software Suite.
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Tap Last week or Last 2 weeks to select either the last week or the last two weeks. To set a custom timeframe, tap From and select a start date from the calendar. Then, tap To and select an end date from the calendar. 6.
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To create a support package and save it on a USB drive, follow the steps below. 1. On the toolbar, tap Tools 2. Tap Data Management. 3. Connect a USB drive to the instrument. Wait for the device to detect the USB drive. A notification is displayed once the drive is connected.
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4. In the Support Package pane, Select Download to USB drive. 5. To set the timeframe of the support package, tap Set timeframe . The default timeframe is the last two weeks. 6. Tap the applicable option for your preferred timeframe. Tap Today to create a support package for the current day.
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8. Tap Create download. A progress bar is shown. To cancel the download, tap the progress bar. Once the download is complete, a notification is displayed, and the USB drive can be removed from the instrument. QIAcuity Safety and Installation Guide 04/2021...
Creating a support package with the QIAcuity Software Suite 1. Select a plate in the plate overview. 2. Click the left side of the screen on Support Package. A new window pops up, where you can specify what kind of support package you want to generate and from which time frame.
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Note: The standard package includes the log files and a combined image view of all wells. The extended package includes the log files and the raw images of each single well. By clicking on the calendar symbol, you can define the time frame from which the support package shall be generated.
Plate Seal is loaded into the instrument. A run cannot be started if a plate seal is not detected by the instrument. Only use QIAGEN products for closing the plates. Drawer blocking If the drawer is retrieved and blocked, please ensure that the plate is correctly loaded into the drawer and parallel to the base surface of the drawer.
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Power OFF the QIAcuity, wait for a few minutes, and power it ON again. Save the file(s) to the USB stick again. Check the USB stick on a PC to ensure it is functional. If the error persists, contact QIAGEN Technical Services.
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Comments and suggestions a PC to ensure it is functional. If the error persists, contact QIAGEN Technical Services. Login screen not visible when If the touchscreen does not display the login screen, but instead a starting instrument software update message is shown, power OFF the QIAcuity and wait for a few minutes.
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Check if correct restriction enzyme was used when using gDNA as template material. Check the starting quality and quantity of the template. We recommend that you use QIAGEN kits for sample preparation. Images are saturated Re-image the plate with 30% lower exposure duration (see also section Image quality control) Sample result is 0 copies/µl or...
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Disc space is critical in the Delete plates from the plate overview. QIAcuity Software Suite User with role Operator forgot Administrator to log-in, new operator can be created. If password administrator forgot the password, contact QIAGEN Technical Service. QIAcuity Safety and Installation Guide 04/2021...
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This error occurs when the data received from the instrument does QIAcuity instrument and software not conform to the expected pattern. Further investigations are required by a QIAGEN Field Service Specialist to diagnose the problem with the instrument. Please contact your distributor or QIAGEN Technical Service.
Accessing the system status and clearing errors Note: Only administrators can access the instrument status. The QIAcuity allows you to see the status of each of its modules. This is especially useful when a hardware error occurs. Details about errors that occurred on the instrument are shown in the System Status section.
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6. Restart the instrument. The instrument initializes and all modules are returned to their home positions. Note: If the affected module is not working after you cleared the error and restarted the instrument, contact QIAGEN Technical Services. WARNING/ Risk of personal injury and material damage...
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The following maintenance procedures must be carried out to ensure reliable operation of the QIAcuity: Regular maintenance Periodic maintenance Optionally, these procedures may be performed to check and ensure the reliability of operation of the QIAcuity. Select the cleaning agent according to the objective of the cleaning procedure, the sample material used, and the downstream assay.
Appendix A – Technical Data QIAGEN reserves the right to change specifications at any time. Operating conditions Power 100–240 V AC, 50/60 Hz, Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages. Maximum power consumption: QIAcuity One, 2plex: 1000VA...
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Pollution level Environmental 3K21 (IEC 60721-3-3) class QIAcuity Safety and Installation Guide 04/2021...
Transport conditions Air temperature –25ºC to 60ºC (–13ºF to 140ºF) in manufacturer’s package Relative 5% to 85% (non-condensing) humidity Environmental 2K11 & 2M4 (IEC 60721-3-2) class Ambient 700hPa to 1060hPa pressure Storage conditions Air temperature 5ºC to 40ºC (41ºF to 104ºF) in manufacturer’s package Relative 5% to 85% (non-condensing) humidity...
Mechanical data and hardware features Width: 60 cm (23.6 in.) Dimensions Height: 58 cm (22.8 in.) Four/Eight Depth: 65 cm (25.6 in.) Width: 38 cm (15.0 in.) Dimensions Height: 45 cm (17.7 in.) Depth: 65 cm (25.6 in.) QIAcuity One: 36.0 kg (79.4 lb.) Mass QIAcuity Four: 43.0 kg (94.8 lb.) QIAcuity Eight: 55.0 kg (121.3 lb.)
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The 2-plex version features the channels Green and Yellow and the 5- Optical plex version all following channels: specifications Channel Green Yellow Orange Crimson Excitation 463–503 514–535 543–565 570–596 590–640 in nm Emission 518–548 550–564 580–606 611–653 654–692 in nm Excitation by high power white LED with average 4750 lumens Image acquisition by CMOS camera with 6.3MP Up to 96 samples per plate.
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Handheld Scan Pattern: Area Image (1280 x 800-pixel array) scanner Motion Tolerance: Up to 89 cm/s (35 in/s) (optional) Print Contrast Ratio: 15% (minimum) Decode Capability: Reads standard 1D, 2D, Postal and stacked codes Resolution: 1D Linear: 0.102 mm/4 mils; PDF417: 0.127 mm/5 mils; Data Matrix: 0.195 mm/7.5 mils QIAcuity Safety and Installation Guide 04/2021...
QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany An up-to-date declaration of conformity can be requested from QIAGEN Technical Services. Waste Electrical and Electronic Equipment (WEEE) This section provides information about disposal of waste electrical and electronic equipment by users.
To recycle electronic equipment, contact your local QIAGEN sales office for the required return form. Once the form is submitted, you will be contacted by QIAGEN either to request follow- up information for scheduling collection of the electronic waste or to provide you with an individual quote.
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Declaration list of China RoHS (SJT 11364-2014) The environmental friendly use period of the QIAcuity instruments is 25 years. The marking for the Restricted Use of Hazardous Substances in Electronic and Electrical Products (SJ/T 11364- 2014) is shown in Table 21. QIAcuity Safety and Installation Guide 04/2021...
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Table 5. Marking for the restricted use of hazardous substances in electronic and electrical products (SJ/T 11364-2014) Part Name - 部件名 Toxic or Hazardous Substances and Element - 有毒或有害的物质成分 称 Lead (Pb) Mercury Cadmium Hexavalent Polybrominated Polybrominated 铅 (Cd) 镉 Chromium biphenyls (PBB) diphenyl ethers...
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Part Name - 部件名 Toxic or Hazardous Substances and Element - 有毒或有害的物质成分 称 Lead (Pb) Mercury Cadmium Hexavalent Polybrominated Polybrominated 铅 (Cd) 镉 Chromium biphenyls (PBB) diphenyl ethers (Hg) 汞 (Cr6+) 六价 (PBDE) 多溴联 多溴联苯 铬 苯醚 Plastics - 塑料 Enclosure/Plastics parts - 外壳/塑料部...
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Document Revision History Date Changes April 2021 Initial release QIAcuity Safety and Installation Guide 04/2021...
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QIAGEN specifically disclaims any other licenses, expressed or implied other than those expressly stated. The purchaser and user of the kit agree not to take or permit anyone else to take any steps that could lead to or facilitate any acts prohibited above. QIAGEN may enforce the prohibitions of this Limited License Agreement in any Court, and shall recover all its investigative and Court costs, including attorney fees, in any action to enforce this Limited License Agreement or any of its intellectual property rights relating to the kit and/or its components.
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Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com QIAcuity Safety and Installation Guide 04/2021...
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