Analog J2 And J6 Series Centrifuges (Models J2-Hs, J2-21, J2-Hc, J6-Hc, And J-6B); Determining The Liquid Pressure; Determining Appropriate Gradients; J2 And J6 Series Centrifuge Temperature Settings. All Settings - Beckman Coulter JCF-Z Instructions For Use Manual

Preparing for Operation

Determining The Liquid Pressure

6
When the entry is correct, press
NOTE
To clear a COMP ADJ entry, press
Table 4.1 J2 and J6 Series Centrifuge Temperature Settings. All settings are approximate.
Speed
(rpm)
20 000
18 000
15 000
10 000

Analog J2 And J6 Series Centrifuges (Models J2-HS, J2-21, J2-HC, J6-HC, and J-6B)

Enter the required run temperature and the appropriate temperature compensation units (see
Table 4.1) on the centrifuge control panel as follows.
1
Turn the
2
Find the compensation value that corresponds to the required temperature and run speed. Set
the
COMP
Determining The Liquid Pressure
A line that delivers liquid to the rotor should be set up with a pressure gauge between the pump and
the centrifuge. At flow rates up to 250 mL/min, pressure may be as high as 10 psi.
Above 500 mL/min, 15 to 20 psi is allowable. Unless your liquid is quite viscous, higher pressures
probably indicate that air is trapped in the passages of the rotor. Trapped air can block liquid flow
and cause tubing to separate from connectors when the pump is operating. The flow rate should not
decrease with increased gauge pressure. (See
Flow Rotor Only)

Determining Appropriate Gradients

Figure 4.2 provides an aid to banding sample in a gradient using the standard pellet core or either
zonal core. According to the figure, the radius at which a zone bands can be determined by
4-6
(
ENTER/RECALL
–20° –10°
N N
N N
N N
N N
knob to the required sample temperature.
SET
dial to that setting. (Interpolate if intermediate values are required.)
for instructions on removing an air block.)
)
.
( ) ( ) (
COMP ADJ , 0 , and ENTER/RECALL
Required Rotor Temperature (°C)
2° 5°
–max –max
–max –max
–10 –9
–6 –6
CHAPTER 5, Purging Air From The System (Continuous
)
.
10° 20°
–max –max
–10 –9
–8 –7
–5 –4
PN JCFZ-IM-12AB
40°
–10
–8
–6
–3