Varian ProStar 325 UV-Vis Driver Manual

Varian ProStar 325 UV-Vis Driver Manual

Detector control
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Varian, Inc.
2700 Mitchell Drive
Walnut Creek, CA 94598-1675/USA
ProStar 325 UV-Vis
Detector Control
Galaxie Driver Manual
©Varian, Inc. 2005
03-914947-44:Rev. 2

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  • Page 1 Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA ProStar 325 UV-Vis Detector Control Galaxie Driver Manual ©Varian, Inc. 2005 03-914947-44:Rev. 2...
  • Page 2: Table Of Contents

    Installing the Driver Software....................3 Varian ProStar 325 Communications Configuration............3 Ethernet Communications.....................3 Configuring the Driver......................4 Configuring the Varian ProStar 325 UV-Vis Detector ...........4 Configuring the Synopsis ....................8 Building a Method ................. 10 Creating a New Method.....................10 Varian ProStar 325 UV-Vis Detector Control Method ............12 Signal Control......................14...
  • Page 3: Introduction

    General This document describes the configuration of the Varian ProStar 325 UV-Vis Detector driver. It also explains how to build a control method for this driver, how to start and view a running acquisition, and what actions are available during an analysis.
  • Page 4: Installation And Configuration

    To install the Galaxie Drivers software refer to the Galaxie installation Guide. Varian ProStar 325 Communications Configuration Ethernet Communications The Varian ProStar 325 UV-Vis Detector uses Ethernet as the method of communications for instrument control. The Varian ProStar 325 UV-Vis Detector uses TCP/IP (Transmission Control Protocol/Internet Protocol) to communicate over the Ethernet network and needs to be given a unique IP address to identify the instrument.
  • Page 5: Configuring The Driver

    The driver software configuration is done in the Galaxie Configuration Manager when a new chromatographic system is created. Configuring the Varian ProStar 325 UV-Vis Detector Click on the button corresponding to the Varian ProStar 325 driver and the configuration screen appears.
  • Page 6 In the IP Address field, enter the IP address of the instrument. Press the button to search the subnet for all connected Varian ProStar 325 instruments. The following window will be displayed. ProStar 325 UV-Vis Detector Control Manual - 5 -...
  • Page 7 Check the Enable Ready In box to enable this option. When this option is enabled the Varian ProStar 325 waits for a ready signal to go to the ready state. NOTE: For more details about the BOOTP configuration, refer to the Ethernet Communications section.
  • Page 8 The detector will always operate in extended range mode with a prep flow cell. ProStar 325 UV-Vis Detector Control Manual - 7 - 03-914947-44:2...
  • Page 9: Configuring The Synopsis

    Galaxie Chromatography Data System, in the Systems tab. In this tab, the Overview part will show the synopsis of the system with some parameters displayed (temperature, flow, signal, etc.) depending on the installed devices. ProStar 325 UV-Vis Detector Control Manual - 8 - 03-914947-44:2...
  • Page 10 For more details about the creation and configuration of a system, refer to the Galaxie Configuration Manager User’s Guide. Click on the button to print the current configuration. ProStar 325 UV-Vis Detector Control Manual - 9 - 03-914947-44:2...
  • Page 11: Building A Method

    1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu. A wizard guide will appear and provide assistance during the first steps of method creation: ProStar 325 UV-Vis Detector Control Manual - 10 - 03-914947-44:2...
  • Page 12 The instrument control section must be defined in order to start an acquisition. In the following part of this guide, only the instrument control section for the Varian ProStar 325 UV-Vis Detector is detailed. Other parameters such as Injection Volume, Vial and Rack number may be necessary and are defined in the acquisition section.
  • Page 13: Varian Prostar 325 Uv-Vis Detector Control Method

    NOTE: For more details about the programming of each method section, turn to the Galaxie Chromatography Data System User’s Guide. Varian ProStar 325 UV-Vis Detector Control Method Once the new method is created, select the control section in the lower part of the browser.
  • Page 14 Click on the button to display the Varian ProStar 325 control method sections. It contains four sub-sections: Signal, Peak Sensor, Relay and Miscellaneous. Click on the required sub-section to define the corresponding parameters that will be used during the chromatographic run.
  • Page 15: Signal Control

    Enter the Response Time in the corresponding field. This parameter specifies the degree of electronic noise filtering ProStar 325 UV-Vis Detector Control Manual - 14 - 03-914947-44:2...
  • Page 16 If Dual Wavelength Mode is checked extra columns are added in the table which will allow the user to enter another Wavelength, Attenuation and an Autozero value. ProStar 325 UV-Vis Detector Control Manual - 15 - 03-914947-44:2...
  • Page 17: Peak Sensor Control

    The Period parameter is the time between Peaksense relay actuations during a time slice event. A relay period of 30 causes the Peaksense relay to actuate every 30 seconds while time slicing is turned on. ProStar 325 UV-Vis Detector Control Manual - 16 - 03-914947-44:2...
  • Page 18 NOTE: For more details about the Peak Sensor functions (Timeslice or Pulse), refer to the appendix (Peak Sensor functions section) at the end of this manual. ProStar 325 UV-Vis Detector Control Manual - 17 - 03-914947-44:2...
  • Page 19: Relay Control

    Use the following buttons to edit the table: to add a line to the table. to delete a line in the table. to clear the entire table. ProStar 325 UV-Vis Detector Control Manual - 18 - 03-914947-44:2...
  • Page 20: Miscellaneous Control

    In the Analog X Peak Ticks scrolling list, select enable or disable. Peak Ticks are small spikes added to the analog output to indicate when the Peak relay is activated. ProStar 325 UV-Vis Detector Control Manual - 19 - 03-914947-44:2...
  • Page 21 Galaxie run. Check the Lamp Off at the end sequence to enable this option. When this option is enabled the Varian ProStar 325 lamps will be turned off at the end of a Galaxie sequence.
  • Page 22: Starting And Viewing An Acquisition

    Quick Start (Single Acquisition) 1. Press the button. In the Quick Start window, select the system, the method and optionally the project if connected in "All project" mode. 2. Press the OK button. ProStar 325 UV-Vis Detector Control Manual - 21 - 03-914947-44:2...
  • Page 23 In a multiple injector system, the same parameters entered for the first injector must be entered for the other injectors before starting the acquisition. ProStar 325 UV-Vis Detector Control Manual - 22 - 03-914947-44:2...
  • Page 24: Sequence

    3. Start the sequence by clicking on the button. NOTE: For more details about how to start an acquisition, refer to the Galaxie Chromatography Data System User’s Guide. ProStar 325 UV-Vis Detector Control Manual - 23 - 03-914947-44:2...
  • Page 25: Running Acquisition Status

    A window is activated which is composed of two parts: the running chromatogram and the status window where the driver modules are listed. Varian ProStar 325 UV-Vis Detector Status Click on the button corresponding to the ProStar 325 driver and the UV-Vis detector status screen will appear.
  • Page 26 The light colors change depending on the detector state (Lamp On/Off, Method Ready/Not Ready/Monitoring, etc.). Click on to start or stop the D2 lamp of the detector. Click on to start or stop the visible lamp of the detector. ProStar 325 UV-Vis Detector Control Manual - 25 - 03-914947-44:2...
  • Page 27 D2 Lamp On Time when a new lamp is installed in the instrument. Click on the button to reset the Vis Lamp On Time when a new lamp is installed in the instrument. ProStar 325 UV-Vis Detector Control Manual - 26 - 03-914947-44:2...
  • Page 28 • Not ready: contains the not ready information sent by the instrument. • Events: contains the events and errors that have occurred on the instrument. ProStar 325 UV-Vis Detector Control Manual - 27 - 03-914947-44:2...
  • Page 29: Appendix

    Appendix Peak Sensor Functions This section describes the two different functions that activate the Peaksense relay of the Varian ProStar 325 UV-Vis Detector. These functions are named Time slice and Pulse Relay. Time Slice Function The Time Slice function is used when you want to partition a selected peak into a number of segments.
  • Page 30: Pulse Relay Function

    Pulse Relay function to advance the sample collector at 2.75 minutes to the next sample receiver). ProStar 325 UV-Vis Detector Control Manual - 29 - 03-914947-44:2...

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