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Summary of Contents for Thermo Scientific Wellwash Versa
- Page 1 Thermo Scientific Wellwash Versa User Manual Rev. 1.3...
- Page 3 Thermo Scientific Wellwash Versa User Manual Rev. 1.3, Cat. No. N11166...
- Page 4 Copyright 2014 Thermo Fisher Scientific Inc. First edition published in 2010. All rights reserved. Decon is a trademark of Decon Laboratories Limited. Microside SQ is a trademark of Global Biotechnologies, Inc. Virkon is a trademark of E.I. du Pont de Nemours and Company or its affiliates. All (other) trademarks are the sole property of Thermo Fisher Scientific Inc.
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Page 5: Intended Users
How to use this This user manual is for the following instruments, Wellwash Versa: Cat. No. 5165010 and 5165050. It has been written to give you the user manual... -
Page 6: Safety Symbols And Markings
Date of manufacture ▲ Consult instructions for use ▲ WEEE symbol This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EC. ▲ 4 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 7: Warning And Other Markings Used In The Documentation
Caution Risk of damage to the instrument, other equipment or loss of performance or function in a specific application. ▲ Note Marks a hint, important information that is useful in the optimum operation of the system, or an item of interest. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 8 About This User Manual Safety symbols and markings 6 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 9: Table Of Contents
How to use this user manual ............. 3 For more information ............... 3 Safety symbols and markings............. 4 Safety symbols and markings used on the Wellwash Versa ..... 4 Warning and other markings used in the documentation ....5 Chapter 1 Introduction to the Wellwash Versa Microplate Washer .... - Page 10 Cell wash................... 71 Aspirate ..................74 Cell aspirate ................75 Dispense ................... 76 Cell dispense ................77 Soak ..................78 Prime ..................79 Pause..................80 Layout..................80 Settings menu ................. 81 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 11 Cleaning the wash head(s)............. 119 Mechanical cleaning of the wash head ........119 Changing or replacing the wash head ..........120 Checking the liquid line ..............120 Checking the residual volume ............120 Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 12 Troubleshooting guide ..............135 Chapter 14 Ordering Information .................137 Wellwash Versa ................137 List of spare parts and accessories ..........137 Appendix A System Log....................139 Appendix B Certificate of Decontamination ...............141 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 13: Introduction To The Wellwash Versa Microplate Washer
In addition, the Wellwash Versa can be connected to plate handling devices. The Wellwash Versa can be used to wash and prepare plates for a variety of test routines, mainly in enzyme-linked immunosorbent assay (ELISA) tests. -
Page 14: Intended Use
It can be used in research or routine-test laboratories by professional personnel. As the Wellwash Versa is part of an analyzing system for the end user, the user is responsible for validation of the whole system to enable production of reliable and safe results. - Page 15 Introduction to the Wellwash Versa Microplate Washer Principle of operation Figure 1–2. Wash heads Figure 1–3. Principle of washing step (Normal) Figure 1–4. Principle of washing step (Sweep2) Figure 1–5. Principle of washing step (Sweep3) Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 16 Introduction to the Wellwash Versa Microplate Washer Principle of operation Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 17: Chapter 2 Installation
Chapter 2 Installation Warning The Wellwash Versa weighs 9 kg [19.8 lbs.] and care must be taken when lifting the instrument. ▲ For more information on main parts of the instrument, refer to Chapter 3: “Wellwash Versa Main Parts”. What to do upon This section covers the relevant procedures to be carried out on receipt of the instrument. -
Page 18: Environmental Requirements
Installation Setups Environmental When you set up your Wellwash Versa, avoid sites with excess dust, vibrations, strong magnetic fields, direct sunlight, draft, excessive requirements moisture, or large temperature fluctuations. Make sure that: The working area is flat, dry, clean and vibration-proof, and leave ●... -
Page 19: Installing The Priming Vessel
If priming vessel the priming vessel is not installed, insert it downwards and pull it towards yourself (Figure 2–8). Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 20: Installing The Wash Head(S)
2. Fit the supplied wash heads to the tubing according to Figure 2–9 through Figure 2–11. Note the difference in sizes. The larger aspiration (waste) tube is connected to the ● bottom hole. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... - Page 21 3. Fit the wash heads next to each other onto the wash head arm with the tips pointing downwards and ensure that the wash heads move freely up and down in the wash head arm slot (Figure 2–11). Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 22 Caution If you use the device with a blank wash head, the dry tubes will cause the pump to operate more loudly. It is recommended to prime both channels to reduce the noise. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 23: Installing The Cell Wash Head
Note Check that the brown adapters of the 2x8 cell wash head are inserted (Figure 2–14). ▲ Note When you replace the cell wash head adapters (Figure 2–14 A), change them both at the same time. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 24: Liquid Bottles And Channels
Figure 2–14. Installing the 2x8 cell wash head onto the wash head arm Liquid bottles and The Wellwash Versa includes three standard buffer bottles (2 liters) and the waste bottle (4 liters). Bottles for greater volumes are available as an channels option (4 l for buffers and 9 l for waste). -
Page 25: Connection Diagram
Caution Before disconnecting the tubing, prime the liquid system with air to avoid liquid spillage. ▲ Figure 2–17 shows the connections of the liquid level sensor cabling and Connection diagram liquid bottle tubing. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 26: Installing The Liquid Bottles
Installation Setups Figure 2–17. Wellwash Versa connection diagram 1. Fit the liquid bottle tubing to the corresponding color-coded tube Installing the liquid connectors on the left side panel of the instrument (Figure 2–18). bottles The connectors are from left to right: W =... - Page 27 W (Waste), R (Rinse), B (Buffer B), and A (Buffer A). Figure 2–19. Connecting the liquid sensor cable All the liquid bottles are connected to the instrument in Figure 2–20. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 28 Caution Check regularly that the liquid filters in the intake tubes are clean. If not, replace or clean them. ▲ Caution To ensure proper function, do not disconnect the sensor cables from the instrument during operation. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 29: Foaming
Warning Do not operate your instrument from a power outlet that has no ground connection. Do not use a power supply cable other than the Thermo Scientific power supply cable designed for your region. ▲ 1. Ensure that the mains (I/O) switch (Figure 2–21) at the right side panel of the instrument is in the off (O) position. -
Page 30: Connecting To A Computer
Power supply connector Figure 2–21. Connecting the power supply cable Connecting to a If you wish to use an external computer with the Wellwash Versa in remote control, connect the communication cable to the USB port for computer PC or serial RS-232 C port (Figure 2–21). -
Page 31: Defects And Abnormal Stresses
The liquid level sensor will warn you if safe levels have been passed. Caution To ensure proper function, do not disable or disconnect sensors. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 32: Mechanical Checks Before Switching On
Check that the outermost tips in the wash head are directly above the indentations to ensure that the wash head arm is at a right angle to the plate carrier. Figure 2–22. Alignment check Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 33: Installing The Aerosol Cover
The liquid level icon should be green when the buffer bottle is upside down and when the buffer bottle is in an upright position. The liquid level Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 34: Settings After Installation
Warning Clogged tips may cause a faulty washing performance. ▲ Settings after To change the wash head type, the preferred user interface language or the date/time, refer to “Settings menu” on page 81. installation Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 35: Wellwash Versa Main Parts
Chapter 3 Wellwash Versa Main Parts This chapter describes the main parts of the Wellwash Versa instrument. Instrument The front view of the Wellwash Versa instrument is shown in Figure 3–24. views Display Keypad Liquid bottles Wash heads on wash head arm... -
Page 36: Instrument Views
Serial RS-232 C port Mains switch USB port for PC Power supply connector Figure 3–25. Wellwash Versa back view The side views of the Wellwash Versa instrument are shown in Figure 3–26. USB port for memory device Connectors for liquid sensor cables,... -
Page 37: Liquid System Diagram
(Cat. No. D08894), is available on request. Plate carrier The plate carrier of the Wellwash Versa instrument supports both portrait and landscape orientation of the 96-well plate and landscape orientation of the 384-well plate (Figure 3–24). A plate clamp is incorporated into the plate carrier to keep the plate in place during processing. -
Page 38: Wash Heads
Warning Do not remove the wash head plugs when in use. ▲ Caution Only use wash heads that have an identification label. ▲ Figure 3–28. Wellwash Versa wash head models Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 39: Shaker
10 Hz, amplitude 1.5 mm High 15 Hz, amplitude 1 mm Caution The instrument is not intended for shaking purposes only. A separate microplate shaker is available if needed (e.g. Thermo Scientific™ iEMS™ Incubator/Shaker). Refer to ▲ www.thermoscientific.com Thermo Fisher Scientific... - Page 40 Wellwash Versa Main Parts Shaker Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 41: Operating The Instrument
Numeric keys with letters and signs; also used for column/strip selection Figure 4–30. Keypad and display of the Wellwash Versa The keys for navigating and editing are detailed below. The keys also have other functions depending on the level in the user interface. - Page 42 Use the keys to select strips. Refer to “Strip selection with the number keys” on page 49. To write an uppercase letter, press the desired letter key repeatedly until the capital appears. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 43: Menus
Settings menu. Refer to “Wash head configuration” on page 84. See the info text bar for the required actions of the 3 keys. The action text on the info text bar changes according to the current menu. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 44: Liquid Level Detection
• bottle is full or disconnected icon is green when the bottle is not yet full • icon is empty with a cross when the LLD is disabled • Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 45: Chapter 5 Running Protocols
Refer to “Priming PRIME the system” on page 47. 6. Select the strips if they are not already selected. Refer to “Strip selection” on page 48. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 46: Filling And Emptying Liquid Bottles
▲ Note Do not remove the plate before the end of the protocol run. ▲ Note It is recommended to shut down the Wellwash Versa at the end of the day. Refer to “Shutting down” on page 109. ▲ Filling and 1. -
Page 47: Starting A Ready-Made Protocol With The Quick Keys
Main menu and select Open using the FILE Down arrow key, and then press the button. Example protocol list 2. Select the ready-made protocol and press the button. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 48: Loading The Plate
(Table 5–3). ▲ Caution Ensure that the strips on a strip plate are pressed flat on the frame. Misaligned strips may increase the residual volume or limit plate carrier movement. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 49: Priming The System
The default volume used during priming is defined in the Settings > Prime & rinse parameters > Prime volume (Prime button) (ml) menu. Refer to “Prime & rinse parameters” on page 81. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 50: Strip Selection
Strip selection can be carried out in two ways: 1. To select the columns/strips of the plate, use the number keys. 2. Press the OK button on the Layout row in the Main menu. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 51: Strip Selection With The Number Keys
Clear all selections: Hold the C / 12 key down for more than ● 2 seconds. Note Strip selection is disabled if the Layout step is the first step of the protocol. Refer to “Layout” on page 80. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 52: Strip Selection With The Layout Row
All selections are cleared with the C key. Columns/strips 1–12 are edited if an 8-way wash head is used or columns/strips 1–8 if a 12-way wash head is used. The selected columns/strips are highlighted with color. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 53: Starting A Run
The volume used during rinsing is defined in the Settings > Prime & rinse parameters > Rinse volume (Rinse button) (ml) menu. Refer to “Prime & rinse parameters” on page 81. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 54 Running Protocols Rinsing the liquid system Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 55: Menus, Tabs And Parameters
Numeric keys with letters and signs; also used for column/strip selection Figure 6–35. Keypad and display of the Wellwash Versa The keys for navigating and editing are detailed below. The keys also have other functions depending on the level in the user interface. - Page 56 Use the keys to select strips. Refer to “Strip selection with the number keys” on page 49. To write an uppercase letter, press the desired letter key repeatedly until the capital appears. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 57: Menus
Settings menu. Refer to “Wash head configuration” on page 84. See the info text bar for the required actions of the 3 keys. The action text on the info text bar changes according to the current menu. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 58: Liquid Level Detection
It is possible to protect protocols from accidental editing or deleting. A locked symbol is shown in front of the protocol name when locked. To lock/unlock a protocol, select it on the protocol list and press the 3 (Lock) key. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 59: Creating A Protocol
If the selected wash head is different from the one that is set in the Settings menu, you must change the wash head in the Settings menu before starting the protocol. Refer to “Wash head configuration” on page 84. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 60 Note Well offset has an effect on the residual volume. ▲ 8. Select the Steps row in the Main menu. Refer to “Steps” on page 9. Press the 3 (New step) key to open the step list box. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 61 Main menu. Note that the strip selection FILE is not saved with the protocol. Use the Layout step if you want to save the strip selection. 12. Select Save As. The Save Protocol As dialog opens. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 62: Creating A Protocol In An Optional Way
1. Select the Protocol row in the Main menu and press the button. 2. Select the Create a new protocol row and press the button. 3. Enter a name for the protocol and press the button. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 63: Adding New Steps To Protocols
Adding new steps to protocols 1. Select the Steps row in the Main menu and press the button. 2. Press the 3 (New step) key to open the step list box. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 64 When there are more than five steps, blue arrows appear at the bottom and/or at the top of the display. 8. Use the Up and Down arrow keys to navigate in the protocol. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 65: Deleting Steps From Protocols
Caution If you edit an existing protocol and change the wash head, the wash head related step parameters of the protocol (e.g. the Wash step) are automatically reset to default values. Soak and Pause steps are not affected. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 66: Well
The following settings are available: Well bottom – Select the well shape. ● Different well shapes of strips and plates available are shown in Figure 6-36. The default well shape format is Flat. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... - Page 67 ▲ Note With Flat bottom wells, setting the well offset off center may decrease the residual volume. This feature should only be used with the Normal aspirate mode. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 68: Microplate Requirements
2 (Close) key to close the window. Both 96- and 384-well format microplates that meet ANSI/SBS Microplate requirements standards can be used with the Wellwash Versa (Table 6–5). Note Use only plates manufactured according to ANSI/SBS standard dimensions. ▲ Table 6–5. Microplate requirements... -
Page 69: Wash
Figure 6–40. Dispense heights The wash step is the step needed in most wash protocols. The well is Wash first emptied, then filled with buffer and finally emptied or left full. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 70 Soak time (mm:ss) – Define the time period between wash cycles in ● the Wash step. The default value is 00:00 (= no soak). The minimum value is 0 s and the maximum value 60 min in increments of 1 s. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 71 Refer to Figure 6–40. Caution Too high an aspirate height increases the residual volume. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 72 ▲ Final aspirate – The wells are emptied at the end of the step. The ● default is Yes, which leaves wells empty. No leaves the wells wet. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 73: Cell Wash
Wash volume (μl) – Define the volume of the wash buffer used in ● microplate washing. The wash volume range is 50 μl to 1000 μl in 10 μl increments. The default value is 250 μl. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 74 96 / C 6.1 mm stays above the bottom. For example, 3.0 mm above the 96 / U 6.1 mm bottom with flat bottom plates. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 75 0.1 mm increments. The default is 0.0 mm. Final aspirate – The wells are emptied at the end of the step. The ● default is Yes, which leaves wells empty. No leaves the wells wet. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 76: Aspirate
If you want the wells entirely empty, move the tips completely to the well bottom or slightly below so that the wash head rests on the bottom of the well. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 77: Cell Aspirate
Caution Too high an aspirate height increases the residual volume. ▲ Caution Aspirating too low may disrupt the cells or block the channels. ▲ Aspirate speed – Define the aspirate rate Low, Medium, or High. ● The default is Low. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 78: Dispense
Ensure that the value fits the plate in use. Because dispensing starts from a lower level in a well and ends at a higher level, the start height should be lower than or equal to the end height. Refer to Figure 6–40. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 79: Cell Dispense
Ensure that the value fits the plate in use. Because dispensing starts from a lower level in a well and ends at a higher level, the start height should be lower than or equal to the end height. Refer to Figure 6–40. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 80: Soak
You can maintain the time between cycles constant with this option. Soak During a Soak step the liquid remains in the wells for the set time with or without shaking of the plate. The Soak step has the following parameters: Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 81: Prime
100 ml in 5 ml increments. The default is 30 ml. Priming is carried out in the priming position. Aspiration is on while priming. At the end, the priming vessel is emptied and the wash head returns to the home position. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 82: Pause
Main > Layout row selection. Note Only the strips that are selected to the Layout step are saved with the protocol. Strips selected using the Layout row are not saved. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 83: Settings Menu
The Settings menu contains the Prime & rinse parameters, Sensors, Wash head configuration, System, and Reports parameters. Prime & rinse The prime and rinse parameters can be set in the Prime & rinse parameters window. parameters Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 84 Autoprime – This primes the instrument at a set interval to ensure ● that the instrument stays primed. Autoprime is not recommended for long time intervals (overnight). Use instead the Soak wash head function. Refer to “Soak wash head” on page 92. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 85: Sensors
Caution If the plate sensor is disabled, there will not be a warning indicating a missing plate. A missing plate may result in spillage. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 86: Wash Head Configuration
Warning An incorrect wash head configuration can cause liquid spillage or a faulty washing performance. ▲ Warning If the error message shown below appears, calibrate the wash head before use. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 87: System
Español (= Spanish) • Italiano (= Italian) • The following information is also visible in the System window: Version: – Version of the software ● SN: – Serial number of the instrument ● Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 88: Reports
Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 89: Maintenance Menu
The procedure cleans or disinfects the instrument according to a set of procedure actions. Refer to Chapter 11: “Maintenance”, particularly “Decontamination procedure” on page 122. To clean or disinfect the instrument: 1. Press the button on the Cleaning/Disinfection procedure row. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 90 4. Press the 2 key to abort the soaking if you want a shorter soak time than the set soak time and to continue the procedure. Priming is then carried out. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 91 2 s. Finally the priming vessel is emptied. 6. Empty all bottles and press the button. Priming is then carried out. 7. Press the button when the procedure has finished. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 92: Clean Wash Head
Note Use a suitable cleaning agent and repeat the Clean procedure with deionized distilled water. ▲ 1. Select the Clean wash head row and press the button. 2. Change the wash buffer to cleaning agent or deionized distilled water. Press the button. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... - Page 93 Priming is then carried out. 6. Press the button when the procedure has finished. Repeat the wash head cleaning procedure with deionized distilled water if cleaning agent has been used. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 94: Soak Wash Head
2. Change the wash buffer to cleaning agent or deionized distilled water if needed. Press the button. 3. Select the priming channel using the Down arrow key and/or press button. Priming, preparing the soak and soaking take place. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 95: Move Wash Head
1. Select the Move wash head row using the Down arrow key and press the button. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 96: Empty Priming Vessel
Move wash head procedure. Refer to “Move wash head” on page 93. 1. Select the Empty priming vessel row using the Down arrow key and press the button. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 97: Calibration
Calibration is performed at 100 μl and 300 μl. The weighed liquid mass of each plate is entered into the Weight fields in the table in grams. The software calculates the internal values of the mean volume per well and Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 98: Starting The Calibration
Note Before running the calibration, make sure that the liquid system operates correctly, for example, that the liquid filters and wash head tips are clean. ▲ Select the Calibration row in the Maintenance menu and press the button. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 99: Active Calibration
The calibration is used for calibrating the wash head. head Note A new wash head of the same kind must be calibrated before use. ▲ 1. Select the Calibrate current wash head row and press the button. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 100 2. Press the button to start the calibration of the wash head. 3. Select the used channel using the Up arrow key and/or press the button. 4. The dispense pump is calibrated first. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 101 6. Weigh the dispensed plate and enter the mass of the dispensed liquid in grams using the number keys. 7. Insert the second tare weighed plate onto the plate carrier and press 1 key to continue. Dispensing is carried out. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 102 ± 50% and that of the second plate must be ± 5%.) 10. Continue dispensing and weighing the third and fourth plate in the same way. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 103 14. Verify the new performance by dispensing a plate, for example, with 300 μl. Tare weigh the plate, dispense and measure the weight to determine the actual dispensed volume. Calculation method: Weight [mg] / 96 = Volume/well [μl] Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 104: Service
Continue with the normal Prime operation. Empty the priming vessel (Maintenance > Clean > Empty priming vessel) if needed. If there is still no liquid flowing, refer to “Manual priming” on page 121. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 105: Chapter 7 Exporting And Importing
Figure 7–41. USB memory device inserted 2. Select the Protocol row in the Main menu and press the button. The list of protocols appears. 3. Select the protocol you want to export. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 106: Importing A Protocol
1. Insert the USB memory device (Figure 7–41). 2. Select the Protocol row in the Main menu and press the button. The list of protocols appears. 3. Press the key to open the File menu. FILE Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 107: Exporting A Protocol As Text File
You can export a protocol as a text file to a USB memory device. The text file has information about the protocol, such as the name, the steps protocol as text and their parameters, modification date, wash head, well type, and well file offset. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 108 The protocol is exported to the USB memory device. The ASCII exported file name is the same as that of the binary file with the .TXT extension. You can open the file with a text editor, print it or save it. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 109: Chapter 8 Deleting
The list of protocols appears. 2. Select the protocol you want to delete. 3. Press the key to open the File menu. FILE 4. Select Delete and press the button. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 110 Deleting Deleting a protocol 5. Select Yes and press the button to confirm the deletion. The wash protocol has now been deleted. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 111: Chapter 9 Shutdown
Chapter 9 Shutdown Shutting down It is recommended to shut down the Wellwash Versa at the end of the day. 1. Remove any plates still in the instrument. 2. Empty the wash bottle(s) and fill them with deionized distilled water. Then prime the instrument. - Page 112 Warning When handling the waste bottle, it is advisable to observe applicable safety precautions, such as the wearing of disposable powder- free gloves, safety glasses, and protective clothing, to avoid potential infectious disease contamination. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 113: Chapter 10 Emergency Situations
3. Carry out appropriate corrective measures. Do not disassemble the instrument. 4. If the corrective measures taken do not help, contact authorized technical service or your local Thermo Fisher Scientific representative. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 114 Emergency Situations Handling abnormal situations Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 115: Regular And Preventative Maintenance
121 and “Calibration” on page 95. Check the aspirate height. See “Checking the aspirate height” on page 121. Replace the tubing if needed. Contact service. Continued Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 116: Instrument Care
▲ Caution Do not use the instrument to aspirate or dispense any strong acidic or alkaline solutions as this could damage the instrument. ▲ Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 117: Cleaning The Instrument
Cleaning the Clean the instrument regularly as stated below. instrument Caution Although the Wellwash Versa is constructed from high-quality materials, you must immediately wipe away spilled saline solutions, solvents, acids, or alkaline solutions from the outer surfaces to prevent damage and wipe them with deionized distilled water. -
Page 118: Cleaning The Plate Carrier
3. If spillages have occurred, move the plate carrier by pushing it to the left (Figure 11–43). This makes it easier for you to clean the area under the plate carrier. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 119: Cleaning Liquid Bottles
(Figure 11–44). Caution Clean or replace the liquid filter of the intake tube regularly. For example, back flush the liquid filter with detergent or use an ultrasonicator. ▲ Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 120: Checking The Liquid Level Sensors
Liquid bottles Blank wash head Liquid bottle caps Liquid filter Intake tubes Liquid level sensor assemblies Priming vessel Sensor nut Tube connectors 1x8 wash heads 1x12 wash heads 1x16 wash head Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 121: Cleaning The Wash Head(S)
Check that the wash head tips are in good condition and not deformed. Mechanical Detach the wash head from the tubing. ● cleaning of the Remove the wash head plugs and use the declogging tool to clean ● the tips. wash head Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 122: Changing Or Replacing The Wash Head
Weigh the plate again and determine the average residual volume per well in μl by dividing the mass in mg by 96. Example: Residual liquid weight 91.2 mg. Residual volume/well [μl] = 91.2/96 [μl] = 0.95 [μl] Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 123: Checking The Dispense Accuracy
5. Close (pinch) the tube, continue pinching the tube and then disconnect the tube from the syringe. 6. Reattach the tube to the fitting on the instrument side and release your grip. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 124: Disposal Of Materials
Warning Inquire about appropriate collection points and approved methods of disposal in your country, state, or region. ▲ Decontamination If you have spilled infectious agents, carry out the decontamination procedure. procedure Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... - Page 125 4. Empty the plate carrier. You must wear disposable powder-free gloves. 5. Switch off the power and disconnect the power supply cable. 6. Disinfect the outside of the instrument using a cloth dampened with 70% ethanol. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
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Page 126: Refitting The Transport Lock
(Figure 11–47). Figure 11–47. Aligning the plate carrier and wash head arm 3. Screw on the transport lock screw and the transport lock tag with the supplied Allen key (Figure 11–48). Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 127: Maintaining A System Log
Refer to Appendix A: “System Log”. How to pack for To pack the Wellwash Versa for service: service Provide information about the use of hazardous materials. ● Prime all the channels of the instrument and clean the wash and ●... -
Page 128: Disposal Of The Instrument
Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department. Disposal of the If the Wellwash Versa is exposed to potentially infectious chemical samples, toxic or corrosive chemicals, or radioactive chemicals, waste instrument management of the complete instrument must be carried out to ensure that there is no risk of contamination. - Page 129 Collect waste from electrical and electronic equipment separately. ▲ Regarding the original packaging and packing materials, use the recycling operators known to you. For more information, contact your local Thermo Fisher Scientific representative. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 130 Maintenance Disposal of the instrument Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 131: Chapter 12 Technical Specifications
User interface Graphical user interface Computer interface USB 1.1 (2.0 compatible), RS 232 Plate types 96- and 384-well plates Maximum height 16.0 mm Number of buffers 2 buffers and 1 rinse buffer Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 132: Performance Specifications
5–100 ml in 5 ml increments Prime volume 5–100 ml in 5 ml increments Memory At least 100 protocols Linear shaker Amplitude 2.5 mm; 5 Hz Amplitude 1.5 mm; 10 Hz Amplitude 1 mm; 15 Hz Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 133: Safety Specifications
Technical Specifications Safety specifications Safety This section describes the safety specifications for the Wellwash Versa instrument. specifications In conformity with Wellwash Versa bears the following markings: the requirements Type 888 100–240 Vac, 50/60 Hz, 100 VA max., Class I CE marking cTÜVus... - Page 134 Technical Specifications Safety specifications Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 135: Chapter 13 Troubleshooting Guide
XY offset adjustment failed. – More than one command has the same hash value. – Parameter memory not found. Contact authorized technical service. Parameter memory erase failure. Contact authorized technical service. Continued Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... -
Page 136: Usb Memory Device
It is recommended to use FAT16 or FAT32 formatted USB memory devices. It is not recommended to use multiple drive USB memory devices that contain virtual CD drives. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific... -
Page 137: Troubleshooting Guide
The multiple drive USB memory devices normally contain one or more removable disk-type memory spaces, but can also contain virtual CD drive-type memory spaces. The Wellwash Versa does not support virtual CD drive-type memory spaces. It is possible to check if there is a virtual CD drive present when inserting a multiple drive USB memory device into the PC USB port. - Page 138 Air leak. Check that the tubes and wash head plugs are properly fitted. Check that the tubing is intact. Apply silicone to the O-ring. Valve is leaking. Contact authorized technical service. Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 139: Ordering Information
2 liter rinse bottle, Wellwash Versa N10810 4 liter waste bottle square N10811 4 liter wash bottle A, round N10812 4 liter wash bottle B, round, Wellwash Versa N10813 4 liter rinse bottle, round, Wellwash Versa N10814 9 liter waste bottle, rectangular, Wellwash Versa N10816... - Page 140 Liquid filter, 5 pcs 2305290 Serial cable F9/F25 SP-00250 PM Kit Wellwash Versa * Longer USB cables available from PC stores Figure 14–50. Cell wash head adapters (A) and wash head tubing connectors (B) Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 141: Appendix A System Log
Appendix A System Log Instrument name/number: User Date Comments Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 142 System Log Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
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Page 143: Appendix B Certificate Of Decontamination
*) The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials. This unit is certified by the undersigned to be free of radioactive contamination. Date and place: Signature: Name (block capitals): Please include decontaminating solution used. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual... - Page 144 Certificate of Decontamination Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific...
- Page 145 384-well plate. A specific 2x8 (cell wash) wash head is used for washing cells. USB Universal serial bus. Index Use the Find option for searching words/information in this user manual. Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual...
- Page 147 Thermo Fisher Scientific Oy Ratastie 2, P.O. Box 100 FI-01621 Vantaa Finland www.thermoscientific.com N11166...
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