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Related Manuals for Thermo Scientific Multiskan Sky
Summary of Contents for Thermo Scientific Multiskan Sky
- Page 1 Thermo Scientific Multiskan™ Sky User Manual Cat. No. N18964 Rev 1.0 2017...
- Page 2 © 2017 Thermo Fisher Scientific Inc. All rights reserved. Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation. This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
- Page 3 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
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Page 5: Preface
• Multiskan™ Sky with Cuvette and Touch Screen + μDrop Plate – Cat. No. 51119700DP, 51119750DP (Japan) and 51119770DP (China) Use Multiskan Sky as standalone (the models with the touch screen) or with Thermo Scientific™ SkanIt™ Software. Multiskan Sky is intended to be used in research laboratories by qualified personnel. -
Page 6: Safety And Special Notices
• Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual (Cat. No. N16046) The instrument software also provides context sensitive help. Safety and special notices Make sure you follow the precautionary statements presented in this guide or on the instrument. The safety and other special notices appear in boxes. -
Page 7: Table Of Contents
Install Multiskan Sky ........ - Page 8 Maintenance checklist ..........34 Thermo Scientific Multiskan Sky User Manual...
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Page 9: Chapter 1 Introduction
Multiskan Sky with the touch screen can be connected to Thermo Fisher Connect cloud-based capabilities or the local area network. Multiskan Sky is designed to be part of an analyzing system for the end user, who is responsible for validating the system to ensure reliable and safe results. - Page 10 (2), measurement chamber door (3), power button (4). The instrument with the cuvette port and measurement chamber door open. Figure 3. Cuvette port (1), plate carrier (2). The back view of the instrument. Thermo Scientific Multiskan Sky User Manual Thermo Scientific...
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Page 11: Principle Of Operation
The USB ports (1) can accept an external memory device, or a Wi-Fi dongle compatible with Microsoft™ Windows™ 10. Principle of operation Multiskan Sky is a monochromator-based microplate and cuvette photometer. The sample on the microplate is measured vertically from bottom to top, while the sample on the cuvette is measured horizontally. - Page 12 Figure 6. Operating principle. Air blank measurement is needed to calculate the absorbance values. In microplate measurement, Multiskan Sky moves the microplate aside to measure the blank automatically. Thermo Scientific Multiskan Sky User Manual Thermo Scientific...
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Page 13: Install Multiskan Sky
Doing so may cause misalignment and will void the instrument warranty. CAUTION Leave the instrument to sit for at least three hours before installing and switching it on to prevent condensation causing a short circuit. Thermo Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 14: Remove The Transport Lock
4. Unfasten the red fixing screw (1) and remove the transport lock (2) from the plate carrier. 5. Push the plate carrier back into the instrument and make sure that the measurement chamber door closes properly. Thermo Scientific Multiskan Sky User Manual Thermo Scientific... -
Page 15: Connect The Mains Supply Cable
CAUTION Do not use other power supply cables than the power supply cable delivered with the instrument. Use the Thermo Scientific power supply cable designed for your region. CAUTION Do not operate your instrument from a power outlet that has no ground connection. -
Page 16: Initial Settings
Initial settings. Initial settings When you switch Multiskan Sky with a touch screen on for the first time, the instrument prompts you to set some initial settings for the instrument. To set the instrument up: 1. Tap Next. -
Page 17: Install Skanit Software
For more information about the software, refer to the Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual. The software automatically detects how Multiskan Sky is configured and shows only those features that are available. Note Your instrument may not have all of the features presented in this manual. -
Page 18: Connect The Software To The Instrument
5. Enter the installation code you received after you registered. Note You can use SkanIt Software for 30 days without the installation code. For detailed installation steps, refer to the Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual. Connect the software to the instrument Connect the software to the instrument by plugging the USB cable from the PC to the instrument. -
Page 19: Application Menu
The actions in the task ribbon are linked to the section you have selected in the Session tree. When you select Plate Layout, Protocol, Results or Report in the Session tree, the relevant task ribbon opens. The task ribbon shows the actions you can select. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 20: Select The Software Language
1. Click Settings in the Application menu. The Settings window opens. 2. Select the language from the drop-down list under General settings. 3. Restart the software to set the new language. Figure 10. Settings window. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... -
Page 21: Chapter 4 Instrument Operations
Touch screen display Multiskan Sky can be operated using the touch screen display of the instrument. CAUTION The touch screen is a sensitive component. Use, for example, your finger to operate the instrument via the touch screen. Do not use a sharp object on the touch screen. -
Page 22: Navigation Bar
Favorites, you can choose to return to either the Home screen, Favorites, or the endpoint measurement parameters under Measure: Tip To return to the Home screen from any view, tap the Home icon. Thermo Scientific Multiskan Sky User Manual Thermo Scientific... -
Page 23: Function Tiles
• Runs: results of previously run measurements • Settings: instrument, network & USB, and μDrop Plate settings Tap the tiles to access the functions. For more information on the different functions, refer to the Thermo Scientific™ Multiskan™ Sky Technical Manual. Application bar The current instrument temperature, Run plate in/out and Power off icons are shown in the application bar at the bottom of the display. -
Page 24: Measure A Cuvette
UV-compatible microplates. 2. Access the measurement from the Home screen. Note If you use Multiskan Sky without the touch screen display, use SkanIt Software to define and start the measurements and to view the results. For instructions, refer to “Using SkanIt Software”... -
Page 25: Air Blanking For Cuvettes
Therefore, when performing very precise measurements, you can perform air blanking manually just before running the measurement. To perform air blanking: 1. Make sure that the cuvette port is empty. 2. Set up the parameters for the cuvette measurement. Thermo Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 26: Instrument Incubator
• by defining in Settings that the files are exported automatically. Note Before you can export files to Thermo Fisher Connect, the instrument must be linked to the cloud and you must be signed in. For instructions, refer to the Thermo Scientific™ Multiskan™ Sky Technical Manual. -
Page 27: Power Save Feature
> Network & USB. You must have write permission to the network share. Power save feature Multiskan Sky has a power save feature which lowers power consumption when the instrument is idle. Enable or disable the power save from Settings. - Page 28 Instrument operations Thermo Fisher Connect cloud-based tools Thermo Scientific Multiskan Sky User Manual Thermo Scientific...
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Page 29: Using Skanit Software
3. Protocol - define what you want the instrument to do (for example, measure, shake). 4. Results - view the measurement results and choose your calculation methods. 5. Report - create a report of the measurement and calculation results. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 30: Plate Layout
You can leave the plate layout empty. The instrument then measures the whole plate automatically. Figure 12. Pipette content section (left.) and virtual pipetting section (right.) in the plate payout. Define the samples for the plate 1. Click Plate Layout in the Session tree. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... -
Page 31: Protocol
Protocol This is where you define which actions the instrument performs.The instrument runs the actions in the order as listed under the protocol. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 32: Protocol Actions
Select the protocol actions from the Protocol ribbon. Note The software automatically detects the instrument configuration and shows only those actions that are available. Figure 16. Protocol ribbon for adding actions. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... -
Page 33: Start A Measurement
This step is skipped if you have previously named the session. 3. Click Save to start the measurement. The software indicates the action it is running. 4. Click the action under Results to watch the measurement results during the run. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 34: Results
2. Click the Plate or List tab to view the results. Figure 20. Results of an absorbance measurement with the Plate view open. Export results to Excel 1. In the Results view click on the Export to Excel tab. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... -
Page 35: Calculations
Figure 21. In this example the Absorbance measurement data is the source data for the Blank Subtraction calculation, and Blank Subtraction data the source for Standard Curve. Calculation actions Select the calculation actions from the Results ribbon. Figure 22. Results ribbon for adding calculations. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 36: Report
You can export any data by selecting the individual result sections to the report. Create a data report 1. Click Report in the Session tree. 2. Check the sections you want to include in the report from the Report sections list. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... -
Page 37: Saved Sessions
A session that is saved before you have run it. A session that you have saved but have not run does not have measurement data. You can edit all of the content. b. A session that has been run. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 38: Open An Existing Session
Exporting a session creates a file with an *.ska extension, which you can import to another PC with SkanIt Software installed. You cannot open the exported session outside SkanIt Software. 1. Open the application menu. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific... - Page 39 4. Select the file and click Open. 5. Click Next. 6. Select the session(s) you want to import and click Finish. Figure 27. Session selection in the Import data window. 7. The imported session opens. Thermo Fisher Scientific Thermo Scientific Multiskan Sky User Manual...
- Page 40 Using SkanIt Software Open an existing session The software adds the imported session(s) to the Open recent session list under New & Recent. Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific...
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Page 41: Safety And Maintenance
2500 V for a 230 V supply and 1500 V for a 120 V supply. Thermo Scientific Thermo Scientific Multiskan Sky User Manual... -
Page 42: Maintenance Checklist
*To save energy, it is recommended to shut down the instrument for the weekends. Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program. Thermo Scientific Multiskan Sky User Manual Thermo Scientific...
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