Troubleshoot Flow Rate Error - illumina MiSeqDx Reference Manual

Troubleshoot Flow Rate Error

The flow rate is the speed in which fluids pass through the fluidics system (µl/min). It is
measured before each run during the pre-run check. If the system is unable to measure the
flow rate, pump a volume of reagent (MiSeqDx SBS Solution (PR2)) through the system
before checking the flow rate again.
1 Use the drop-down list or on-screen keyboard to enter the following information:
• Solution: PR2
• Volume: 250 µl
• Aspirate Rate: 2500 µl/min
• Dispense Rate: 2500 µl/min
2 Select Pump.
Figure 47
3 When the pump step is complete, select Restart Check.
4 If the error persists, set the volume to pump 500 µl MiSeqDx SBS Solution (PR2) and
repeat the process 1 more time. Contact Illumina Technical Support if the second
attempt does not resolve the error.
MiSeqDx Instrument Reference Guide
Measure Flow Rate
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