illumina NovaSeq 6000 Manuals

illumina NovaSeq 6000 Manuals

Manuals and User Guides for illumina NovaSeq 6000. We have 1 illumina NovaSeq 6000 manual available for free PDF download: Manual

illumina NovaSeq 6000 Manual

illumina NovaSeq 6000 Manual (87 pages)

Sequencing System

Brand: illumina | Category: Laboratory Equipment | Size: 1.58 MB

Table of Contents

Table of Contents
6
Chapter 1 Overview

8
- Introduction
  8
- Additional Resources
  9
- Sequencing Overview
  9
- Sequencing Workflow
  10
- Instrument Components
  12
Chapter 2 Kits and Accessories

17
- Kits Overview
  17
- Reagent Kit Components
  18
- Novaseq Xp Kit Components
  21
- Novaseq Xp Flow Cell Dock
  22
- Symbol Descriptions
  23
Chapter 3 Getting Started

25
- Start the Instrument Configure Settings User-Supplied Consumables and Equipment
  25
Chapter 4 Standard Workflow: Preparing Consumables

34
- Methods
  34
- Library Guidelines
  34
- Thaw SBS and Cluster Cartridges
  34
- Empty Used Reagent Bottles
  35
- Prepare Flow Cell
  37
- Pool and Denature Libraries for Sequencing
  37
Chapter 5 Novaseq Xp Workflow: Preparing Consumables

41
- Novaseq Xp Workflow Summary
  41
- Methods
  42
- Library Guidelines
  42
- Thaw SBS and Cluster Cartridges
  42
- Empty Used Reagent Bottles
  43
- Prepare Flow Cell
  45
- Pool, Denature, and Load Libraries for Sequencing
  45
Chapter 6 Sequencing

54
- Set up a Sequencing Run
  54
- Monitor Run Progress
  59
- Delete the Run
  60
- Detach Position #30
  60
- Automatic Post-Run Wash
  61
Chapter 7 Maintenance

62
Appendix A Troubleshooting

67
- Troubleshooting Resources
  67
- Troubleshooting Files
  67
- Pre-Run Check Errors
  67
- Process Management Troubleshooting
  68
- Run Failure before Clustering
  68
- End a Run
  69
- Shut down the Instrument
  70
Appendix B Real-Time Analysis

71
- Real-Time Analysis Overview
  71
- Real-Time Analysis Workflow
  73
Appendix C Output Folders and Files

76
- Sequencing Output Folder Structure
  76
- Sequencing Output Files
  77
Appendix D Windows Security

78
- Security Configurations
  78
- Password Requirements
  78
- Windows Firewall
  78
- Enhanced Mitigation Experience Toolkit
  78
- Software Restriction Policies
  79
- Index
  82
- Technical Assistance
  86
- Document # 1000000019358 V07 Material # 20023471
  87

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