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Qiagen EZ1 Advanced XL User Manual

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EZ1
®
User Manual
9001874
QIAGEN GmbH
QIAGEN Strasse 1
40724 Hilden
GERMANY
1108598
R2
Sample to
Insight__
Advanced XL
November 2017

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  • Page 1 November 2017 Advanced XL ® User Manual 9001874 QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden GERMANY 1108598 Sample to Insight__...

  • Page 2: Table Of Contents

    Heat hazard ....................13 UV radiation ....................13 2.10 Maintenance safety ..................14 2.11 Symbols on the EZ1 Advanced XL instrument ..........16 2.11.1 Location of symbols ................18 General Description ....................19 External features of the EZ1 Advanced XL ............20 3.1.1 EZ1 Advanced XL door .................20 3.1.2...
  • Page 3 4.3.1 Unpacking the EZ1 Advanced XL ............31 4.3.2 Installing the EZ1 Advanced XL ..............35 4.3.3 Configuring and setting up the EZ1 Advanced XL ........36 4.3.4 Installing the printer ................37 4.3.5 Installing the bar code reader ..............37 Installing the PC software................38 4.4.1...
  • Page 4 Opening and closing the EZ1 Advanced XL door ..........49 5.4.1 Opening the EZ1 Advanced XL door............49 5.4.2 Closing the EZ1 Advanced XL door ............49 Starting and stopping a protocol run ...............50 5.5.1 Starting a protocol run ................50 5.5.2 Stopping a protocol run ................51 Setting up the worktable ................52...
  • Page 5 5.14 Using the EZ1 Advanced Communicator software ........73 5.14.1 User interface ..................73 5.14.2 Report file in pdf format .................76 5.15 Using multiple EZ1 Advanced XL instruments ..........76 5.16 Evaluating pipetting accuracy ..............76 Maintenance ......................77 Regular maintenance procedure ..............78 Daily maintenance procedure ................81 Weekly maintenance procedure ..............82...

  • Page 6: Introduction

    1 Introduction Thank you for choosing the EZ1 Advanced XL. We are confident it will become an integral part of your laboratory. Before using the instrument, it is essential to read this manual carefully and to pay particular attention to any advice it contains concerning hazards that may arise from use of this instrument.

  • Page 7: Intended Use Of Ez1 Advanced Xl

    The EZ1 Advanced XL is designed to perform automated isolation and purification of nucleic acids. It is intended to be used only in combination with QIAGEN kits indicated for use with the EZ1 Advanced XL instrument for the applications described in the kit handbooks.

  • Page 8: Safety Information

    2 Safety Information This user manual contains information about warnings and cautions that must be followed by the user to ensure safe operation of the EZ1 Advanced XL instrument and to maintain the instrument in a safe condition. Possible hazards that could harm the user or result in damage to the instrument are clearly stated at the appropriate places throughout this user manual.
  • Page 9 EZ1 Advanced XL. After the EZ1 Advanced XL has been unpacked, 2 people must lift the instrument. Lift the instrument by placing your hands underneath the bottom of the instrument. Note: In case of emergency, switch off the EZ1 Advanced XL instrument and unplug the power cord from the power outlet. CAUTION...

  • Page 10: Electrical Safety

    Note: If operation of the instrument is interrupted in any way (e.g., due to interruption of the power supply or a mechanical error), first switch off the EZ1 Advanced XL instrument, then disconnect the electrical cord from the power supply and contact QIAGEN Technical Services.

  • Page 11: Environment

    To ensure proper ventilation, maintain a minimum clearance of 15 cm (6 in.) and 30 cm (12 in.) from the rear and sides of the EZ1 Advanced XL. Slits and openings that ensure the ventilation of the instrument must not be covered.

  • Page 12: Biological Safety

    2.6 Chemicals WARNING Hazardous chemicals Some chemicals used with the EZ1 Advanced XL instrument may be hazardous or may become hazardous after completion of a purification. Always wear safety glasses, gloves, and a lab coat. The responsible persons (e.g., laboratory manager) must take the necessary...

  • Page 13: Toxic Fumes

    2.9 UV radiation The EZ1 Advanced XL supports 2 UV lamps. The wavelength of the UV light produced by the UV lamp is 253.7 nm. This wavelength corresponds to ultraviolet light type C, which can be used for decontamination procedures.

  • Page 14: Maintenance Safety

    The UV lamp consists of a robust glass cylinder. It is safely located inside the door of the EZ1 Advanced XL. In case of accidental breakage of the lamp, contact QIAGEN Technical Services or your local distributor to arrange proper disposal of the lamp.
  • Page 15 Improper use of the EZ1 Advanced XL instrument may cause personal injuries or damage to the instrument. The EZ1 Advanced XL must only be operated by qualified personnel who have been appropriately trained. Servicing of the EZ1 Advanced XL instrument must only be performed by QIAGEN Field Service Specialists.

  • Page 16: Symbols On The Ez1 Advanced Xl Instrument

    2.11 Symbols on the EZ1 Advanced XL instrument The following symbols appear on the EZ1 Advanced XL instrument or in this user manual. Symbol Location Description Heating system Heat hazard — the temperature of the heating system can reach up to 95ºC.
  • Page 17 On the cover of the user manual Catalog number. On the cover of the user manual Material number (i.e., component labeling). On the cover of the user manual Revision number of the user manual. EZ1 Advanced XL User Manual 11/2017...

  • Page 18: Location Of Symbols

    2.11.1 Location of symbols Heat hazard symbol on the heating system. Biohazard and heat hazard symbols near the tip rack. UV radiation hazard symbol on the back of the instrument. EZ1 Advanced XL User Manual 11/2017...

  • Page 19: General Description

    EZ1 Advanced XL worktable. The user then closes the EZ1 Advanced XL door and starts the protocol. The door locks automatically at the start of the protocol. The protocol provides the necessary instructions for the EZ1 Advanced XL to carry out automated nucleic acid purification.

  • Page 20: External Features Of The Ez1 Advanced Xl

    3.1.1 EZ1 Advanced XL door The EZ1 Advanced XL door can be manually opened by the user in order to gain access to the worktable when no protocol is running. A protocol cannot be started until the EZ1 Advanced XL door is closed.

  • Page 21: Ez1 Advanced Xl Card Slot

    3.1.3 EZ1 Advanced XL card slot The EZ1 Advanced XL card slot accepts the EZ1 Advanced XL card. Each EZ1 Advanced XL card contains one or more protocols that allow the EZ1 Advanced XL to perform nucleic acid isolation. CAUTION Damage to the instrument Only use QIAGEN EZ1 Advanced XL cards with the EZ1 Advanced XL.

  • Page 22: Status Leds

    Note: Do not insert the EZ1 Advanced XL card while the EZ1 Advanced XL is switched on. EZ1 Advanced XL cards must not be exchanged while the EZ1 Advanced XL is switched on. Take care not to expose the EZ1 Advanced XL card to electric shock, water or dirt.

  • Page 23: Internal Features Of The Ez1 Advanced Xl

    RS-232 connectors. The left connector is used to connect the bar code reader. The right connector is used to connect the EZ1 Advanced XL to a PC or an RS-232 printer. Note: Do not use items other than the attached accessories 3.2 Internal features of the EZ1 Advanced XL...
  • Page 24 Sarstedt (cat. no. 72.693), and provided in EZ1 Kits. Tip rack. Both tip holders and filter-tips are made of polypropylene and are provided in EZ1 Kits. The filter-tips have a capacity of 50–1000 μl. Tip holders and filter-tips. EZ1 Advanced XL User Manual 11/2017...
  • Page 25 Heating system The heating system is located under the far end of the cartridge rack. It holds the heating positions of the reagent cartridges. EZ1 Advanced XL User Manual 11/2017...

  • Page 26: Pipettor Head

    Located behind the tip adapters is the piercing unit, a row of 14 metal spikes for puncturing the foil that covers the reagent cartridges. During operation, the piercing unit, controlled by the EZ1 Advanced XL, opens the reagent cartridges. The pipettor head then automatically picks up filter-tips from the tip rack and performs aspiration and dispensing operations at different locations on the worktable before ejecting the tips back into the tip rack.

  • Page 27: Uv Lamps

    3.2.3 UV lamps The EZ1 Advanced XL is equipped with 2 UV lamps, located under the blue EZ1 Advanced XL door. The UV lamps can be manually switched on and off in a separate menu, as described later in this manual (see Section 5.7.1).
  • Page 28 UV lamps and reflector case. UV lamps Location of UV lamps under the EZ1 Advanced XL door. CAUTION Damage to the instrument Be careful not to damage the cover of the UV lamps when loading and unloading the worktable. EZ1 Advanced XL User Manual 11/2017...

  • Page 29: Installation Procedures

    EZ1 Advanced XL is equipped with a 3-conductor AC power cord that, when connected to an appropriate AC power outlet, grounds (earths) the EZ1 Advanced XL. To preserve this protection feature, do not operate the EZ1 Advanced XL from an AC power outlet that has no ground (earth) connection.

  • Page 30: Installation Of Ac Power Cord

    4.2.3 Installation of AC power cord Connect one end of the AC power cord to the socket located on the rear of the EZ1 Advanced XL and the other end to the AC power outlet. Note: Do not use items other than attached accessories.

  • Page 31: Hardware Installation

    O-ring (pack of 14) and silicon grease  Fuses (1 each: 6.3 A and 3.15 A)  LED Type Handy Bar code reader (CSA approved)  EZ1 Advanced XL User Manual (this user manual)  EZ1 Advanced XL teaching data sheet  Packaging list ...
  • Page 32 5. Pull out the 4 corner shock absorbers at the top, and remove the outer cardboard box by pulling it up. 6. Take out the wrapped EZ1 Advanced XL, and remove the aluminum strap. EZ1 Advanced XL User Manual 11/2017...
  • Page 33 EZ1 Advanced XL Plastic sheets Inner bottom cardboard Note: Be careful not to damage the surface of the EZ1 Advanced XL after removing the plastic sheets. Note: It is recommended to save the original packaging material for later transportation of the EZ1 Advanced XL.
  • Page 34 Removing the protectors for the Y- and Z-axes During transportation, protectors prevent the moveable parts of the EZ1 Advanced XL from moving along the Y- and Z-axes. Before using the instrument, these protectors must be removed. 1. Remove the silica gel bags.

  • Page 35: Installing The Ez1 Advanced Xl

    3. Connect the handheld bar code reader to the EZ1 Advanced XL (Section 4.3.5, page 37). 4. Connect the PC to the EZ1 Advanced XL. Use the RS-232 cable and plug it into the RS-232 connector labeled “PC/Printer” at the rear of the instrument (Section 3.1.5, page 22). Make sure that the connector is secured with the 2 screws provided.

  • Page 36: Configuring And Setting Up The Ez1 Advanced Xl

    Setting the installation date The first time the EZ1 Advanced XL is switched on, it is necessary to enter the installation date. This date will be recorded in the report file described below. The installation date is also used to let the instrument know when to show the weekly and annual maintenance reminders.

  • Page 37: Installing The Printer

    The bar code reader is powered through the data cable. Unpack the bar code reader. Connect the cable to the bar code reader. Connect the bar code data cable to the EZ1 Advanced XL. The connector is located at the rear of the instrument, labeled “Barcode Reader” (Section 3.1.5, page 22). Make sure that the connector is secured with the 2 screws provided.

  • Page 38: Installing The Pc Software

    4.4 Installing the PC software The EZ1 Advanced software, EZ1 Advanced Communicator, needs to be installed on the PC. The software is needed to receive report files from EZ1 Advanced XL and to store data on the PC hard disk.

  • Page 39: Installing The Driver Of The Usb-Rs-232 Converter

    CD and following the instructions given. The following installation wizard window appears. 2. Click Next Mapping the COM port 1. Click the Start button on your PC and select Settings/Control Panel. 2. Double-click System. 3. Click the Hardware tab. EZ1 Advanced XL User Manual 11/2017...
  • Page 40 COM port list. Look for the COM port named USB to Serial Bridge. 7. Right click on this COM port and select Properties. 8. Click the Port Setting tab and select the Advanced button. EZ1 Advanced XL User Manual 11/2017...

  • Page 41: Installing The Ez1 Advanced Communicator Software

    3. Check the COM1 box. The EZ1 Advanced Communicator software is now ready to receive data from the EZ1 Advanced XL. In order to check the connectivity between the PC and EZ1 Advanced XL, use the test described in Section 5.9.3.

  • Page 42: Installing Multiple Ez1 Advanced Xl Instruments

    4.5 Installing multiple EZ1 Advanced XL instruments Up to 4 EZ1 Advanced XL instruments can be connected to a single PC. It is also possible to connect a combination of EZ1 Advanced XL and EZ1 Advanced instruments. The maximum number of instruments that can be connected to a single PC is 4.
  • Page 43 The connectors are labeled on the top side of the connector housing. P1 – P4 3. Connect the other end of the RS-232 serial cables to the EZ1 Advanced XL or EZ1 Advanced. See Section 3.1.5 for more information. Use the connector for PC/printer.

  • Page 44: Transportation And Removal Of The Ez1 Advanced Xl

    Section 4.3.1 in reverse order. Use original packaging material. Appendix A gives information about transportation conditions. If the EZ1 Advanced XL needs to be sent to QIAGEN, please contact your local instrument service organization. Follow the steps below. 1. Decontaminate the instrument.

  • Page 45: General Operation

    For details, refer to the handbook supplied with the EZ1 kit you are using. If you want the EZ1 Advanced XL to generate a report file, you will be asked to enter additional data.

  • Page 46: Inserting The Ez1 Advanced Xl Card

    1. Open the cover of the EZ1 Advanced XL card slot. 2. Insert the EZ1 Advanced XL card into the EZ1 Advanced XL card slot. 3. Orientate the card so that the picture faces to the left and the triangle symbol points towards the EZ1 Advanced XL Card slot.

  • Page 47: Removing The Ez1 Advanced Xl Card

    5. Close the cover of the EZ1 Advanced XL card slot. 6. Switch on the EZ1 Advanced XL (Section 5.3.1, page 48). Note: Do not remove the EZ1 Advanced XL Card while the machine is switched on. 5.2.2 Removing the EZ1 Advanced XL card 1.

  • Page 48: Switching The Ez1 Advanced Xl On And Off

    2. Switch on the EZ1 Advanced XL at the rear power switch. 3. The display shows the following screen for a few seconds. This text indicates that the EZ1 Advanced XL is initializing. All modules move to their home positions.

  • Page 49: Opening And Closing The Ez1 Advanced Xl Door

    5.4 Opening and closing the EZ1 Advanced XL door 5.4.1 Opening the EZ1 Advanced XL door Reach under the door and push it up until the upper end slides into position. The door will be automatically held open. 5.4.2 Closing the EZ1 Advanced XL door 1.

  • Page 50: Starting And Stopping A Protocol Run

    5.5 Starting and stopping a protocol run 5.5.1 Starting a protocol run After you have inserted the EZ1 Advanced XL card and switched on the EZ1 Advanced XL, start a protocol as follows. 1. Press START on the control panel.

  • Page 51: Stopping A Protocol Run

    2. If you want the EZ1 Advanced XL to generate a report file, you will be asked to enter additional data, such as user ID, bar code of the kit, and bar code of the samples. 3. Open the EZ1 Advanced XL door, and set up the worktable according to the messages shown in the display.

  • Page 52: Setting Up The Worktable

    The tray is located under the worktable and can be removed for cleaning. 1. Switch off the EZ1 Advanced XL. 2. Remove the tip rack. 3. Push the worktable toward the back of the EZ1 Advanced XL. 4. Remove the tray using its handle. EZ1 Advanced XL User Manual 11/2017...

  • Page 53: Loading The Reagent Cartridges

    2. Tap the reagent cartridges until the reagents are deposited at the bottom of the wells. 3. Remove the cartridge rack from the worktable. EZ1 Advanced XL User Manual 11/2017...
  • Page 54 Note: If fewer than 14 cartridges are used, they may be loaded in any order on the rack. However, when loading the other labware, make sure that they follow the same order. 6. Return the cartridge rack to the worktable. EZ1 Advanced XL User Manual 11/2017...

  • Page 55: Loading The Elution Tubes, Filter-Tips And Sample Tubes

    4. Label the elution tubes before you load them into the rack. Make sure that the caps of the tubes are removed before you start the protocol. 5. Return the tip rack to the worktable. EZ1 Advanced XL User Manual 11/2017...

  • Page 56: Using The Uv Lamps

    Note: UV decontamination helps to reduce possible pathogen contamination of the EZ1 Advanced XL worktable surfaces. The efficiency of inactivation has to be determined for each specific organism and depends, for example, on sample layer thickness and sample type. QIAGEN cannot guarantee complete eradication of specific pathogens.
  • Page 57 UV lamp cooling Please stand by For user safety, the UV lamp cools for approximately 3 minutes. The EZ1 Advanced XL door cannot be opened until after the cooling time has elapsed. After cooling, the main menu appears. EZ1 Advanced XL User Manual 11/2017...

  • Page 58: Switching Off The Uv Lamps

    5.7.4 UV lamps ignition error If one or both of the UV lamps fail to ignite, the EZ1 Advanced XL will retry 2 more times. If the retries do not ignite the lamp, the following error message appears. ERROR: UV Lamp...

  • Page 59: Manual Operation

    Axis START: Run ESC: Back Axis indicates either the ALL, Y, Z, P or M function. Press START to start the “home axis” operation or ESC to return to the previous screen. EZ1 Advanced XL User Manual 11/2017...

  • Page 60: Manual Operation "Return Tip

    ENT: Done Open the EZ1 Advanced XL door, and clean the piercing unit as described in Section 6.1, page 78. After cleaning the piercing unit, close the EZ1 Advanced XL door and press ENT to finish the procedure.

  • Page 61: Manual Operation "Resend

    Manual operation “resend” If a report file cannot be sent to the PC or printer, it is temporarily stored on the EZ1 Advanced XL. Up to 10 report files can be stored. Use the “resend” function to manually transmit the report file(s) to the PC or printer.

  • Page 62: Test Function

    1: Axis 2: Temp 3: Serial 4: Version ESC: Back The following test procedures will be carried out by the EZ1 Advanced XL. Move modules to their home positions.  Pick up tip 2 and release it.  Pick up tip 1 and move it to sample position and Proc 1 position.
  • Page 63 Proc 5 Proc 4 Proc 3 Proc 2 Proc 1 Sample Tip 2 Tip 1 Eluate Proc positions tested by the EZ1 Advanced XL test procedure. First 3 rows shown of 14 in total. EZ1 Advanced XL User Manual 11/2017...

  • Page 64: Test Heating Block

    5.9.2 Test heating block This function tests if the heating block of the EZ1 Advanced XL is operating. Press 2 in the test screen to choose the “Temp” operation. TEST 1: Axis 2: Temp 3: Serial 4: Version ESC: Back The following screen appears.
  • Page 65 If the transmission is successful, the result will show PASSED; if not successful, it will show FAILED. Printer Press START to send a test string to the printer. The following screen appears after transmission is complete. TEST: PC/Printer Target: Printer Result: COMPLETED ESC: Back Printout of the printer test. EZ1 Advanced XL User Manual 11/2017...

  • Page 66: Test Version

    2: Temp 3: Serial 4: Version ESC: Back The following screen appears. TEST: VERSION Firmware: VersionNo ESC: Back VersionNo indicates the current version of the firmware. Press ESC to return to the test screen. EZ1 Advanced XL User Manual 11/2017...

  • Page 67: System Setup

    Press 2 in the system setup menu to change the time. SYSTEM SETUP 1: Date 2: Time 3: SerialPort 4: PM ESC: Back The following screen appears. SETUP: TIME HH: MM: SS Up, Dn, SHIFT: Set ENT: Next ESC: Back EZ1 Advanced XL User Manual 11/2017...

  • Page 68: Setting The Serial Port

    5.10.4 Setting the annual maintenance reminder Press 4 in the system setup menu to change the setting of the maintenance reminder. SYSTEM SETUP 1: Date 2: Time 3: SerialPort 4: PM ESC: Back EZ1 Advanced XL User Manual 11/2017...

  • Page 69: Using The Bar Code Reader

    5.12 Using the external printer Printers sold in the open market can be connected to the instrument. The EZ1 Advanced XL allows you to confirm that a report was printed correctly before the report file is deleted. The following screen appears.

  • Page 70: Generating A Report File

    Press 1 to confirm that the print quality is satisfactory. The report file in the EZ1 Advanced XL will then be deleted. Alternatively, press 2 if the print quality is not satisfactory. The report file will be printed again. An example report file is shown below.
  • Page 71 Current firmware version Firmware version Installation date of instr. Jan 10, 2017 Installation date, set the first time the EZ1 Advanced XL is powered; stored permanently on the EZ1 Advanced XL Feb 10, 2017 When you accept the weekly Weekly maintenance done...
  • Page 72 Parameter Example output Description Trace Additional protocol name (specifies the protocol if there are multiple protocols on the EZ1 Advanced XL card) Date of run: Mar 14, 2017 Time stamp for date of run given through internal clock and calendar...

  • Page 73: Using The Ez1 Advanced Communicator Software

    (see Section 5.8.4, page 61). Up to 10 report files can be stored temporarily on the EZ1 Advanced XL. When additional files are stored, the oldest report files will be deleted. The following screen appears.
  • Page 74 The Status Message panel displays the name of the last report file that was sent. Select File to exit the graphical user interface. Select Options to display the following window showing the EZ1 Advanced XL serial ports. COM1 should already be selected.
  • Page 75 Select About in the main window to show the version of the EZ1 Advanced Communicator software. Click the Change path button in the main window to change the folder where the selected report file is stored. The following window opens. Browse and select the folder. EZ1 Advanced XL User Manual 11/2017...

  • Page 76: Report File In Pdf Format

    5.15 Using multiple EZ1 Advanced XL instruments Up to 4 EZ1 Advanced XL instruments can send report files to a single PC. For this configuration, only the QIAGEN PC can be used. For information about installing multiple EZ1 Advanced XL instruments, see Section 4.5.

  • Page 77: Maintenance

    The next time the EZ1 Advanced XL is switched on, the reminder screen will appear again. The screen will continue to appear each time the EZ1 Advanced XL is switched on, until you press 1 to confirm that the maintenance has been completed.

  • Page 78: Regular Maintenance Procedure

    Only perform maintenance as described in this user manual. Servicing Each EZ1 Advanced XL is supplied with a one-year warranty that includes all repairs due to mechanical breakdown. Worldwide, the maximum time for response to a breakdown is 5 days.
  • Page 79 WARNING/ Risk of personal injury and material damage Improper use of the EZ1 Advanced XL may cause personal injuries or CAUTION damage to the instrument. WARNING Samples containing infectious agents Some samples used with this instrument may contain infectious agents. Handle such samples with the greatest of care and in accordance with the required safety regulations.
  • Page 80 Open door and clean piercing unit ENT: Done 6. Open the EZ1 Advanced XL door, and wipe the piercing unit using a soft tissue moistened with 70% ethanol. The piercing unit is sharp. Two pairs of gloves are recommended. Piercing unit 7.

  • Page 81: Daily Maintenance Procedure

    2: Man 3: Test 4: Setup 10. Open the EZ1 Advanced XL door. Clean the tray and racks with 70% ethanol and then with distilled water. See Section 5.6.1, page 52 for removing the tray. 11. Clean the EZ1 Advanced XL worktable with 70% ethanol and then with distilled water.

  • Page 82: Weekly Maintenance Procedure

    1. Apply a small amount of silicon grease to the end of a filter-tip. 2. Apply the silicon grease to the surface of the O-rings. 3. Place the tip onto the pipettor head, and rotate the tip on the pipettor head to distribute the silicon grease evenly. EZ1 Advanced XL User Manual 11/2017...

  • Page 83: Reagents For Decontamination

    EZ1 Advanced XL system. Use according to manufacturers’ instructions for effective disinfection. WARNING Toxic fumes Do not use bleach to clean or disinfect the EZ1 Advanced XL instrument. Bleach in contact with salts from the buffers can produce toxic fumes. EZ1 Advanced XL User Manual 11/2017...
  • Page 84 Damage to the instrument Do not use spray bottles containing alcohol or disinfectant to clean surfaces of the EZ1 Advanced XL instrument. Spray bottles should be used only to clean items that have been removed from the worktable. * This is not a complete list of suppliers and does not include many important vendors of biological supplies.

  • Page 85: Troubleshooting

    7 Troubleshooting 7.1 EZ1 Advanced XL door errors If the EZ1 Advanced XL door is open when you start a protocol run, the following error screen will be displayed. ERROR: Door open! Close the door and retry. ESC: Retry Close the door and press ESC to continue with the protocol run.

  • Page 86: Error Codes

    Record the error code and the line number, and contact QIAGEN Technical Services. Then reset the EZ1 Advanced XL by following the procedure given below. 1. Press ESC to display the main menu. 2. Ensure that the EZ1 Advanced XL door is closed.
  • Page 87 Door open when starting an operation. Bottom sensor of pipettor head (Z-axis) activated during an operation. Communication error between heating block and temperature controller, or card is not an EZ1 Advanced XL Card. Communication error between motors and driver board. –...

  • Page 88: Glossary

    A metal rack that accommodates reagent cartridges on the worktable. Connector panel The panel on the rear of the EZ1 Advanced XL. It contains the power switch, the socket for the power cord, the fuse box, and a connector for a computer cable.
  • Page 89 Sarstedt (cat. no. 72.693), and provided in EZ1 Kits. Tip adapter One of 14 metal probes installed on the pipettor head. During operation of the EZ1 Advanced XL, the tip adapters pick up filter- tips from the worktable. Tip holder A polypropylene tube that holds a single filter-tip.

  • Page 90: Technical Data

    Appendix A Technical data QIAGEN reserves the right to change specifications at any time. Operating conditions Power 100–120 V AC, 50/60 Hz, 600 VA (North America and Japan) 200–240 V AC, 50/60 Hz, 600 VA (Europe) Mains supply voltage fluctuations are not to exceed 10% of nominal supply voltages.

  • Page 91: Storage Conditions

    48 kg (128 lb.) Instrument features Automated nucleic acid isolation using magnetic particles  Desktop instrument  Protocols stored on EZ1 Advanced XL Cards  To be used only with QIAGEN EZ1 kits  Aspirates and dispenses 14 samples or reagents simultaneously ...
  • Page 92 Attach to the tip adapters of the pipettor head to allow liquid aspiration and dispensing. Capacity of 50–1000 μl. The EZ1 Advanced XL accommodates up to 28 tip holders, each containing a filter-tip, in the tip rack on the worktable.

  • Page 93: Waste Electrical And Electronic Equipment (Weee)

    Recycling can be provided by QIAGEN upon request at additional cost. In the European Union, in accordance with the specific WEEE recycling requirements and where a replacement product is being supplied by QIAGEN, free recycling of its WEEE-marked electronic equipment is provided.

  • Page 94: Fcc Declaration

    QIAGEN GmbH, Germany is not responsible for any radio or television interference caused by unauthorized modifications of this equipment or the substitution or attachment of connection cables and equipment other than those specified by QIAGEN GmbH, Germany.

  • Page 95: Declaration Of Conformity

    QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden Germany We herewith declare under our sole responsibility that the product EZ1 Advanced XL; cat. no. 9001874 Annex III Conformity Assessment Route: Other IVD Device Classification: meets all applicable requirements of the following European Directives:...

  • Page 96: Liability Clause

    Representations and warranties made by any person, including representatives of QIAGEN, which are inconsistent or in conflict with the conditions in this warranty shall not be binding upon the Company unless produced in writing and approved by an officer of QIAGEN.

  • Page 97: Index

    Software, 73 decontamination, 83 installation, 38, 41 reminder, 36, 77 Storage conditions, 91 schedule, 77 Switching off, 48 weekly, 82 Switching on, 48 Manual operation Symbols clean, 60 location, 18 home axis, 59 safety, 16 EZ1 Advanced XL User Manual 11/2017...
  • Page 98 28 heating block, 64 Ventilation, 11 serial port, 64 VFD, 20 version, 66 Warnings, 8 Tip adapters, 26 Waste disposal WEEE, 93 Tip holders, 24 Worktable, 23 Tip rack, 23 setting up, 52 EZ1 Advanced XL User Manual 11/2017...
  • Page 99 (Schülke & Mayr GmbH); IBM ® (IBM Corporation); Microsoft ® , Windows ® , Windows Vista ® (Microsoft Corporation); Sarstedt ® (Sarstedt AG and Co.). HB-0176-002 1108598 11/2017 © 2009–2017 QIAGEN, all rights reserved EZ1 Advanced XL User Manual 11/2017...
  • Page 100 Ordering www.qiagen.com/shop | Technical Support support.qiagen.com | Website www.qiagen.com EZ1 Advanced XL User Manual 11/2017...

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