BIO RAD Mini-PROTEAN Quick Start Manual

ELECTROPHORESIS
Mini-PROTEAN
Quick Start Guide
Instructions for Using Mini-PROTEAN Precast Gels
1. Remove Comb: Position thumb on indentation
(middle of comb) and remove comb by pulling
upward in one smooth motion.
2. Remove Tape: Pull the green tape gently to
remove it from the bottom of the cassette.
3. Assemble Mini-PROTEAN Tetra Cell:
Assemble the cassette into the running module
of the Mini-PROTEAN Tetra system. Add
running buffer to the inner and outer chambers.
Use a syringe or a disposable transfer pipet to
rinse the wells with running buffer.
4. Run Gel: Prepare the samples and load
into the wells. If using Bio-Rad Precision
Plus Protein ™ standards, load 10 µl (5 µl
for Precision Plus Protein
standards). Run the gel until the dye front
reaches the reference line. Refer to the
instruction manual (#1658100) for more
information on running conditions. At the
completion of the run, disconnect the cell
and remove the cassette.
5. Open Cassette: Align the arrow on the
opening lever (catalog #456-0000) with the
arrows marked on the cassette. Insert the
lever between the cassette plates at the
indicated locations and apply downward
pressure to break the seal. Gently pull apart
the two plates beginning from the top of
the cassette. Note: Opening lever is 100%
aluminum and recyclable.
6. Remove Gel: Gently remove the gel from
the cassette.
Please note: when running 1– 2 gels in the
Mini-PROTEAN Tetra cell, do not leave the
companion module in the tank; do not run
different gel types (chemistries) or percentages
at the same time.
®
™ WesternC ™
Precast Gels
Preparing the Cassette
Running the Gel
Opening the Cassette
Remove comb
Reference line
to monitor run
Remove tape
Align arrow on the
cassette with arrow
on the opening lever