Performing A Volume Test - illumina MiSeq System User Manual

Performing a Volume Test

An obstruction in the fluidics lines might be the cause of poor reagent delivery or
sequencing results. If an obstruction in the fluidics lines is suspected, perform a volume
test.
A volume test checks the health of the fluidics system by estimating the volume between
two bubbles as they pass by the sensors. To perform a volume test, the wash tray and wash
bottle must be loaded with laboratory-grade water and a used flow cell must be in place.
Follow the onscreen prompts to perform the test.
1 Make sure that a used flow cell is loaded on the instrument.
2 From the Manage Instrument screen, select System Check.
3 Select Conduct Volume Test, and then select Next.
Figure 45
4 Fill each reservoir of the wash tray with 6 ml laboratory-grade water.
5 Fill the 500 ml wash bottle with 350 ml laboratory-grade water.
6 Load the wash tray and wash bottle onto the instrument.
a Open the reagent compartment door and reagent chiller door, and slide the wash
tray into the reagent chiller until it stops. Close the reagent chiller door.
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System Check Screen
Part # 15027617 Rev. H