Olympus BX45 Instructions Manual
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Olympus BX45 Instructions Manual

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INSTRUCTIONS
BX45
ERGONOMIC MICROSCOPE
This instruction manual is for the Olympus Ergonomic Microscope Model BX45. To ensure the
safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,
we recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 4 8 3
This publication is printed on 100% recycled paper

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  • Page 1 BX45 ERGONOMIC MICROSCOPE This instruction manual is for the Olympus Ergonomic Microscope Model BX45. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

  • Page 3: Table Of Contents

    BX45 CONTENTS Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble the microscope yourself, please read section 8, “ASSEMBLY” (pages 28 to 30) carefully. IMPORTANT — Be sure to read this section for safe use of the equipment. —...
  • Page 4 OPTICAL CHARACTERISTICS ASSEMBLY — See this section for the replacement of the light bulb. — 28-30 ■ PROPER SELECTION OF THE POWER SUPPLY CORD ..............31-32...

  • Page 5: Safety Precautions

    UIS2/UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX series and objectives/eyepieces for the UIS series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum performance may result if inappropriate accessories are used.
  • Page 6 Microscope frame rear panel position (Warning against high temperature) Should warning stickers become soiled, peeled off, etc., contact Olympus for replacement. Getting Ready 1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

  • Page 7: Maintenance And Storage

    BX45 Maintenance and Storage 1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute alcohol.

  • Page 8: Nomenclature

    NOMENCLATURE }If you have not yet assembled the microscope, read section 8, “ASSEMBLY” (pages 28 to 30). Interpupillary distance adjustment scale (Page 13) Marker U-MARKER Allen screwdriver (accommodation position) Diopter adjustment ring (Page 13) Condenser centering screws (accommodation positions) Slider insertion slot Main switch (Page 1) Slide holder (Page 11) Voltage indicator LED...
  • Page 9 Phase contrast/darkfield ring mount (Pages 18 - 20) Filter mount (Bottom side) (Page 9) Mountable devices Mountable filters (32 mm diameter) Optical devices for phase contrast · 32C (provided), (32LBD)* · BX45-PH1 · 32LND6 · BX45-PH2 · 32LND25 · BX45-PH3 · 32IF550...

  • Page 10: Transmitted Light Brightfield Observation Procedure

    TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE }As the phase contrast, darkfield and simplified polarized light observations using transmitted light requires preparation using optical devices such as an analyzer or polarizer, they will additionally be detailed in section 4, “OBSERVATION”. (Controls Used) (Page) @Main switch Set the main switch to “...
  • Page 11 BX45 ‹ (Trinocular observation tube only) ™ ‰ † ƒ š ³ … ² œ › Š ‡ Œ } Copy the observation procedure pages on separate sheets and post it near your microscope.

  • Page 12: Using The Controls

    USING THE CONTROLS 3-1 Base Voltage Indication (Fig. 4) 1. Turn the brightness adjustment knob @ clockwise to increase the volt- age and make illumination brighter. 2. The numerals ² around the knob indicate the approximate voltage. ² Fig. 4 Using the Light Intensity Preset Switch (Fig.
  • Page 13 BX45 Mounting a Single Filter (Fig. 6) One of the filters listed below can be engaged in the light path by insert- ing the filter @ in the filter slider ² and engaging the filter slider in the ² light path.

  • Page 14: Focusing Block

    3-2 Focusing Block # The stage of this microscope is set at a low position. Take care not to let your hand interfere with the stage when operating the coarse adjustment knob. Replacing the Fine Adjustment Knob (Fig. 9) ƒ # The fine adjustment knob is attached to the right side when the microscope is shipped from the factory.

  • Page 15: Stage

    BX45 3-3 Stage Placing the Specimen ² # The dimensions of the slide glass should be 26 x 76 mm with thick- ness of 0.9 to 1.4 mm, and the cover glass should have thickness of 0.17 mm. # When observing very large specimens, remove the slide holder and place the specimen directly on the stage.

  • Page 16: Rotating The Stage

    Adjusting the X- and Y-Axis Knob Tension (Fig. 15) 1. Hold the X-axis knob @ and slide up the Y-axis knob ² up to expose the ² adjustment knobs. 2. Turning the X-axis adjustment knob ³ or Y-axis adjustment knob | clock- wise (in the direction of the arrow) increases the tension and counter- clockwise decreases it.

  • Page 17: Observation Tube

    BX45 3-4 Observation Tube Adjusting the Interpupillar Distance (Fig. 17) While looking through the eyepieces, adjust for binocular vision until the left and right fields of view coincide completely. The index dot · indicates the interpupillary distance. }Note your interpupillary distance so that it can be quickly duplicated.

  • Page 18: Using Eyepiece Micrometer Disks

    Using Eyepiece Micrometer Disks (Fig. 20) When the WHN10X-H (or WHN10X) eyepieces are used, an eyepiece micrometer disk can be inserted in one of them. When the eyepiece does not have a diopter adjustment mechanism, however, it is hard to focus on the micrometer disk if the operator has poor eyesight.

  • Page 19: Condenser

    BX45 3-5 Condenser Centering the Condenser (Figs. 23 - 25) 1. Turn the condenser height adjustment knob @ to raise the condenser to its upper limit, then use the top lens swing-out lever ² move the top lens into the light path.

  • Page 20: Condenser

    Example: With the UPlanFLN40X (NA 0.75), set the scale to 0.75 x 0.8 = 0.6. N.A. scale Fig. 27 Compatibility of Objectives and Condensers Objective Magnification BX45 Condenser 1.25X - 4X * Applicable by swing the top lens out. 4X -60X Applicable by engaging the top lens in the light path.

  • Page 21: Immersion Objectives

    BX45 3-6 Immersion Objectives # Be sure to use the provided Olympus Immersion oil. Using Immersion Objectives (Fig. 28) 1. Focus on the specimen with objectives in the order of lower-power to higher-power ones. 2. Before engaging the immersion objective, place a drop of provided immersion oil onto the specimen at the area to be observed.

  • Page 22: Marker (U-Marker)

    3-8 Marker (U-MARKER) }Mounting the marker @ on a 10X objective allows you to mark the de- sired positions with a simple, one-touch operation. Marking can be controlled by either the left or right hand, depending on how the marker is installed. (For details, refer to the Instruction Manual.) }If you want objectives for cytological observation, please purchase the PlanN10XCY and PlanN series objectives or the PlanFLN10XCY and...
  • Page 23 BX45 Observation Procedure (Figs. 33 - 35) # Disengage the analyzer and polarizer from the light path, and re- place the objective with a Ph objective. 1. Engage the Ph objective in the light path. 2. Turn the turret @ to engage the suitable PH ring for the Ph objective in the light path.

  • Page 24: Transmitted Light Darkfield Observation

    }The optical device (DF ring) can be mounted in the same way as the optical device (PH ring). Please see page 18. Observation Procedure # Disengage the polarizer and analyzer from the light path. 1. Turn the turret (@ in Fig. 33) to engage the BX45-DFA in the light path. 2. Engage an applicable objective in the light path. UIS2 Series...

  • Page 25: Transmitted Light Simple Polarization Observation

    BX45 4-3 Transmitted Light Simple Polarization Observation }Polarized light observation requires the U-ANT analyzer (or any module incorporating an analyzer) and the BX45-PO polarizer. Mounting the BX-45PO Polarizer (Fig. 36) 1. Pull out the filter slider @. 2. Insert the BX45-PO polarizer ² into the hole all the way until stopped.

  • Page 26: Troubleshooting Guide

    Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Problem...
  • Page 27 BX45 Problem Cause Remedy Page f ) One side of image is blurred. Objective is not correctly engaged in light Make sure that revolving nosepiece – – path. clicks into place correctly. Stage is not correctly mounted. Re-attach it. – –...
  • Page 28 Problem Cause Remedy Page a) Field of view of one eye does not Your view is not accustomed to micro- Upon looking into eyepieces, try look- match that of the other. scope observation. ing at overall field before concentrat- ing on specimen range. You may also –...

  • Page 29: Specifications

    BX45 SPECIFICATIONS Item Specification 1. Optical system UIS2/UIS (Universal Infinity System) optical system (featuring infinity correction) 2. Illumination Built-in transmitted Koehler illumination 6V 30W halogen bulb (pre-centered) 6V30WHAL (PHILIPS 5761) (Average life time: Approximately 100 hr. when used as directed) Light intensity voltage range: 2 V or less to 5.9 V DC (continuous)

  • Page 30: Specifications

    Item Specification 7. Condenser Objective range · Transmitted brightfield: 1.25X to 100X and applicable · Transmitted PH: 10X to 100X objective pow- · Transmitted darkfield: Any objective with NA of no more than 0.7. · Transmitted polarized: 2X to 100X (NOTE: When using a 1.25X to 4X objective, swing out the top lens.) The NA in widefield observation is 22 in any mode.

  • Page 31: Optical Characteristics

    Iris: Iris diaphragm NOTE length Field number (FN) Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces Color band Cover glass thickness and objectives that can be combined with this microscope.

  • Page 32: Assembly Diagram

    The module numbers shown in the following diagram are merely the typical examples. For the modules with which the module numbers are not given, please consult your Olympus representative or the catalogues. # When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass surfaces.
  • Page 33 BX45 8-2 Detailed Assembly Procedures Installing the Bulb (Figs. 38 & 39) Use only the designated bulb 6V30WHAL (PHILIPS 5761). 1. Holding the bulb @ with gloves or a piece of gauze, insert the bulb pins ² straight and fully into the pin holes ³ on the lamp socket.
  • Page 34 ” (OFF) before con- ³ necting the power cord. Always use the power cord provided by Olympus. IF no power cord is provided with the microscope, please select the proper power cord by referring to section “PROPER SELECTION OF THE POWER SUPPLY ²...

  • Page 35: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 36 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
  • Page 37 MEMO...
  • Page 38 MEMO...
  • Page 40 Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 31 Gilby Road, Mt.

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