After Run Procedures - GE AKTA avant User Manual

5 Operation
5.9 Create a method and perform a run

5.9.4 After run procedures

5.9.4 After run procedures
Introduction
This section describes how to clean the instrument and columns after a chromatographic
run, and how to prepare the system for storage.
The instrument and the columns should be cleaned between the runs. This will prevent,
for example, sample contamination, protein precipitation and column clogging. If the
instrument is not going to be used for a couple of days or longer, the instrument, columns
and the pH flow cell should be filled with storage solution. For further information about
cleaning and maintenance procedures, see Chapter 7 Maintenance, on page 262.
Tip:
System cleaning
After a method run is completed, perform the following:
•
•
•
•
•
•
236
To clean and fill the instrument and columns with storage solution, use the
System CIP and Column CIP methods. Either as separate, predefined methods
or as phases included in a chromatographic method.
WARNING
Corrosive chemicals during maintenance. If the system or column
is cleaned with a strong base or acid, flush with water afterwards
and wash with a weak neutral buffer solution in the last step or
phase.
Rinse the instrument with one or several cleaning solution(s) (e.g., NaOH, buffer solu-
tion or distilled water) using the System CIP method.
If applicable, empty the fraction collector.
Clean all spills on the instrument and on the bench using a moist tissue.
Empty the waste vessel.
Clean the manual injection port of the injection valve, see Clean the manual injection
port of the injection valve, on page 294 for detailed instructions.
If applicable, clean the pH electrode manually and make sure to leave it in an appro-
priate buffer. See Section 7.6.6 Storage of the pH electrode, on page 305 for detailed
instructions.
ÄKTA avant User Manual 29035184 AE