Sample Application - GE AKTA avant User Manual

5.7

Sample application

Introduction
This section describes the different sample application techniques that can be used with
ÄKTA avant. The table below shows the alternatives for sample application available in
the Sample application phase of a method.
Sample applica-
tion
Inject sample di-
rectly onto column
Inject sample from
loop
Note:
When using a pump for sample application, it is important to prime inlets and purge the
pump before using the pump to load the sample. See further instructions in Section 5.4.2
Prime sample inlets and purge Sample Pump, on page 173.
When loading sample using an external air sensor, the sensor should be installed accord-
ing to Adapter for air sensor, on page 49.
ÄKTA avant User Manual 29035184 AE
Via
Sample pump
•
Sample pump and air
•
sensor
Syringe
•
Sample pump
•
In order to avoid sample carry-over when switching techniques for loading
samples, wash the valve with buffer between the loading of two different
samples. For example, when switching from loading sample in the loop to
loading sample directly onto the column with the valve in Direct inject position.
5 Operation

5.7 Sample application

Compatible loops
Not applicable
•
Sample loop
•
Superloop, 10 ml
•
Superloop, 50 ml
•
Superloop, 150 ml
•
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