Sterilization Objects - Sanyo MLS-3751 Instruction Manual

Sterilization Objects

•
Do not sterilize objects in a steam-impermeable container or bag.
•
Do not seal the object container tightly. Use a porous cap or
loosen the cover sufficiently.
•
Do not allow the sterilization objects to block the holes or
temperature sensor in the chamber.
Sterilization and melting of liquids such
as culture mediums, etc.
•
Capacity of culture mediums and containers
Pour less liquid in the container than the specified volume.
Excessive liquid will spill when heated.
•
Sterilization of large volume culture mediums
There is a time lag between when the temperature in the chamber
reaches the sterilization temperature and when the temperature of
the whole culture medium reaches the sterilization temperature.
For this reason, this time lag has been included in the sterilization
time in advance. Therefore, take the time lag into account when
setting the sterilization time.
Sterilization Setting Time = Sterilization Time + Time Lag
Liquid Volume
Agar media in 500ml flask x 1~21, or
Agar media in 1000ml flask x 1~12
Recommendation
An Object temperature sensor
of objects to be sterilized and controls sterilization time is available
as an option. Even after this sensor has been installed, control can
be switched over to the product's own sensor by pressing the
object temperature ON/OFF button. Use this optional sensor to
ensure sterilization is more reliable. For details, contact your
dealer.
•
Sterilization with the use of plastic containers
Prolong the sterilization setting time even more. Otherwise, poor
heat conduction of the container may cause incomplete
sterilization.
•
Melting of solidified general agar mediums
Refer to the below table to set the melting time (per flask).
Capacity Melting Temperature
2 L
1 L
•
Test of Durham tube
Air remaining in the Durham tube can be purged by setting the
exhaust temperature and exhaust rate to appropriate levels.
WARNING
Time Lag
MLS-3751
8min.
that detects the temperature
Melting Time
100°C
Temperature
121°C
100°C
MLS-3781
11min.
60min.
45min.
Porous caps
Sterilization
setting time
Sterilization
Time lag
time
Temperature inside
the chamber
Culture medium
temperature
Time
Plastic
container
Durham tube
12