GE AKTA pilot 600 User Manual page 52

3 Description of modules
3.9 Conductivity monitor
As variation in temperature influences conductivity readings, the conductivity flow cell
is fitted with a temperature sensor that measures the temperature of the eluent. A
temperature compensation factor is used to report the conductivity in relation to a set
reference temperature. The measured temperature is also used for pH electrode com-
pensation.
Pre-column conductivity monitor
If a conductivity monitor is installed in the flow path before the column valve, it can be
used
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Conductivity monitor
(post-column)
The conductivity monitor after the column can be used
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to follow conductivity during washing of pumps and valves before the column,
to monitor that the sample feed has suitable conductivity for the chromatography
run,
to monitor that column equilibration is completed by comparing conductivity levels
before and after the column,
to monitor that all sample has been wash out from the air trap by comparing con-
ductivity of the sample and buffer used to finalize sample application (if using the
air trap during sample application).
to measure the conductivity of salt gradients during gradient elution,
to monitor washing of the system, with the column(s) by-passed,
to monitor that column equilibration is completed by comparing conductivity levels
with column in-line and by-passed,
to follow peak positions relative to conductivity.
ÄKTA pilot 600 User Manual 29274559 AA