Calibrate The Uv Monitors - GE AKTA avant User Manual

7.7.4

Calibrate the UV monitors

Automatic wavelength
calibration of the UV Monitor
The wavelength is automatically calibrated every time the instrument is switched on. If
the instrument has been switched on for a couple of days, and the ambient temperature
and/or the humidity has changed, restart the instrument using the power switch to cal-
ibrate the UV Monitor U9-M.
Calibration of the second UV
monitor flow cell length
The cell path length of the second UV Monitor U9-L might differ from the nominal length,
which leads to incorrect results in the calculation of protein concentration in the eluate.
To achive normalized absorbance, the path length of the second UV flow cell must be
calibrated.
Note:
Equipment needed
To perform the calibration, a calibration kit containing test solutions, syringes and acces-
sories is needed. A specified kit is available for each cell length.
If using a UV flow cell
with the theoretical
path length...
2 mm
5 mm
To calculate the real path length of the UV flow cell, use the following software:
•
ÄKTA avant User Manual 29035184 AE
The flow cell path length must be registered or updated in UNICORN, when
the flow cell is replaced.
UV-900 cell calibration Excel-file (Product Code 18-6324-06)
Then use calibration kit...
UV-900 2 mm calibration kit (Product Code 18-6324-02)
UV-900 5 mm calibration kit (Product Code 18-6324-04)
7 Maintenance
7.7 Calibration procedures
7.7.4 Calibrate the UV monitors
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