Connect A Column - GE AKTA avant User Manual

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5.5 Connect a column

Introduction

This section describes how to connect a column to the instrument using a column

holder and without introducing air into the flow path. Several types of column holders

are available for the ÄKTA avant instrumen, see Section 2.6 Accessories, on page 43.

Methods automatically include pressure alarms based on the specifications of the chosen

column type. However, when running manual runs you have to set the pressure limits

yourself. Also, to protect the column media, special settings are needed. See Section 5.6

Set pressure alarms, on page 187 for more information on pressure alarms.

Before connecting a column, read the instructions for use of the

column. To avoid exposing the column to excessive pressure, make

sure that the pressure limit is set to the specified maximum pressure

for the column.

Do not overtighten when connecting columns. Overtightening might rupture

the connectors or squeeze the tubing and thereby result in high back pressure.

ÄKTA avant User Manual 29035184 AE