Hitachi L-2100 Instruction Manual page 10

1.1 Isocratic System
(1) An eluent is fed by the pump through the degasser.
(2) A sample is injected from the autosampler.
(3) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(4) Each component separated from the sample is then
detected as a signal by the detector.
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