HP D300 Operating Manual page 37

Figure 5-6 Set Titration dialog box
Select the type of titration to use in the Titration Options area of the dialog box. Choose
e.
from the following options:
○
General titration: you set both lowest and highest concentrations or volumes and let the
middle concentrations or volumes fall along either a linear or log scale (as selected)
○
Specific titration: you set the highest or lowest concentrations or volumes and select one
of the following specific types of concentration or volume spacing:
○
1/2 log
○
1/3 log
○
1/4 log
○
1:1 (50% dilution)
○
1:2 (33% dilution)
○
1:3 (25% dilution)
Type in the highest and/or lowest well concentration or volume within the valid
f.
concentration range shown in the lower-left portion of the Set Titration dialog box.
Type the number of replicates at each well concentration or volume level in the Titration
g.
Definition area of the dialog box, for example type ―3‖ for a triplicate titration.
NOTE: The lowest possible well concentration is determined by the minimum drop volume, the
fluid concentration, and the assay volume. The maximum well concentration is determined by the
DMSO limit and the fluid concentration.
4. Additional fluids can be added to the same wells using the same steps as above. If multiple
fluids are in a single well, the well will have two or more colors in relative proportion to
represent all the fluids that will be dispensed into the well as shown in
HP D300 Digital Dispenser
Direction
area
General Type area
Fluid drop-down
list box
Figure 5-7.
37