User Manuals: Seahorse XF96 Cell Analysis Equipment

Manuals and User Guides for Seahorse XF96 Cell Analysis Equipment. We have 1 Seahorse XF96 Cell Analysis Equipment manual available for free PDF download: Installation And Operation Manual

Seahorse XF96 Installation And Operation Manual (145 pages)

Extracellular Flux Analyzer and Prep Station

Brand: Seahorse | Category: Oxygen Equipment | Size: 13.79 MB

Table of Contents
5
Introduction

9
1 Seahorse Bioscience XF96 Instrument Overview

9
2 Technical Specifications

10
3 XF Instrument, Controller and PS Installation Procedure

12
4 Basic Operation of the XF96 Instrument

30
Performing the XF Assay

40
5 Seahorse Bioscience Cell Culture Overview

40
9 Operation of the Prep Station for Media Exchange

42
XF Experimental Results: Using the XF Data Viewer

49
12 Basic Operation of the XF Data Viewer and Graphic Analysis

49
- Well Grid Features and Options
  51
- Basic Graphing Features and Options
  53
13 Analysis of XF Experimental Data - General Guidelines

57
- Basic Analysis
  57
- Intermediate Analysis
  58
15 Metformin-Enhanced Fatty Acid Oxidation Experiment Using C2C12 Myoblast Cells

63
Appendices

75
The XF Assay Wizard and Creating XF Assay Templates

75
- General Tab
  75
- Cells Tab
  75
  - Media and PPR Tab
    77
  - Compounds Tab
    78
  - Background Correction Tab
    79
  - Groups and Labels Tab
    80
  - Protocol Tab
    81
  - About MIX, Wait and Measure Times
    83
OCR, ECAR and PPR: Information on Rates Calculations

85
Group Statistics and AUC Analysis

93
Advanced Graphing Options and Features

104
Reagent and Solution Preparation

134
- Preparation of Palmitate-BSA Complex
  140

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User Manuals: Seahorse XF96 Cell Analysis Equipment

Seahorse XF96 Installation And Operation Manual (145 pages)

Table of Contents

Introduction

1 Seahorse Bioscience XF96 Instrument Overview

2 Technical Specifications

3 XF Instrument, Controller and PS Installation Procedure

4 Basic Operation of the XF96 Instrument

Performing the XF Assay

5 Seahorse Bioscience Cell Culture Overview

9 Operation of the Prep Station for Media Exchange

XF Experimental Results: Using the XF Data Viewer

12 Basic Operation of the XF Data Viewer and Graphic Analysis

13 Analysis of XF Experimental Data - General Guidelines

15 Metformin-Enhanced Fatty Acid Oxidation Experiment Using C2C12 Myoblast Cells

Appendices

The XF Assay Wizard and Creating XF Assay Templates

OCR, ECAR and PPR: Information on Rates Calculations

Group Statistics and AUC Analysis

Advanced Graphing Options and Features

The Media, Cell, & Compound Database and Additional Features and Options

Reagent and Solution Preparation

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