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SpectraMax M5e
Molecular Devices SpectraMax M5e Manuals
Manuals and User Guides for Molecular Devices SpectraMax M5e. We have
2
Molecular Devices SpectraMax M5e manuals available for free PDF download: User Manual
Molecular Devices SpectraMax M5e User Manual (86 pages)
Multi-Mode Microplate Readers
Brand:
Molecular Devices
| Category:
Laboratory Equipment
| Size: 0.72 MB
Table of Contents
Table of Contents
3
Chapter 1 Description
7
Introduction
7
Applications
8
Certified Spectramax
8
M5 E -HTRF Readers
8
Optics
8
Dynamic Range
8
Spectramax M5
8
Pathcheck Pathlength Measurement Technology
9
Automix
9
Temperature Control
9
Supported Plates
9
Computer Control
10
Data Collection and Display
10
Data Reduction and Plotting
10
Immediate Results Reporting and Analysis
10
Instrument Control
10
Reader Components
11
The Control Panel
12
Temp
13
Temp On/Off
13
Wavelengths
13
Drawer
14
Mode
14
Read Cuvette
14
The Microplate Drawer
15
Microplates
16
Cuvettes
17
The Cuvette Chamber
17
The Back Panel
18
Chapter 2 Principles of Operation
19
Absorbance
19
Optical Density
19
Transmittance
19
Pathcheck® Pathlength Measurement Technology
19
Water Constant or Cuvette Reference
22
Background Considerations
22
Pathcheck Pathlength Measurement Technology and Interfering Substances
23
Normalizing Absorbance Measurements
24
Fluorescence
24
Time-Resolved Fluorescence (M4, M5, and M5E Only)
27
Fluorescence Polarization (M5 and M5E Only)
28
Luminescence
28
Functional Description
29
Temperature Regulation
29
Read Types
30
Endpoint Read
30
Kinetic Read
30
Spectrum Read
30
Well Scan Read
31
Automix
31
Computer Control
31
Chapter 3 Installation
33
Unpacking
34
Setting up the Instrument
35
Installing the Drawer Adapter
36
Removing the Drawer Adapter
37
Chapter 4 Operation
39
Cuvette Read-Quick Overview
39
Microplate Read-Quick Overview
40
Preparing for a Cuvette or Microplate Reading
40
Turn the Instrument and Computer on
40
Set the Temperature (Optional)
41
Select the Wavelength
42
Read the Cuvette
43
Read the Microplate
43
Optimizing Fluorescence Assays
44
Optimizing Absorbance Assays
44
Excitation and Emission Wavelengths
44
Emission Cutoff Filter
45
Readings Per Well
45
PMT Voltage
45
Temperature Control
45
Using Spectral Scanning to Optimize Excitation and Emission Wavelengths for Fluorescence Assays
46
Optimizing Time-Resolved Fluorescence Assays
50
Optimizing Fluorescence Polarization Assays
51
Optimizing Luminescence Assays
52
Chapter 5 Maintenance
53
Technical Support
53
Moving a Spectramax Multi-Mode Microplate Reader
55
General
55
Cleaning
56
Cleaning the Fan Filter
57
Changing the Fuses
57
Chapter 6 Troubleshooting
61
Opening the Drawer Manually
61
Error Codes and Probable Causes
62
Error Messages
62
Appendix A Specifications
67
Spectramax Multi-Mode Microplate Reader Performance Specifications
67
Microplate Readers Include the M3, M4, M5, and M5
67
E Models. When a
67
E Models Only)
69
System Diagrams and Dimensions
73
Common Fluorescence and Luminescence Wavelengths
74
Fluorescence
75
Time-Resolved Fluorescence
75
Luminescence
75
Appendix B Cables and Accessories
77
Cables
77
Serial Interface Cable
77
USB Adapter Cable
77
Accessories
78
Cuvettes
78
Standard and Semi-Micro Cuvettes
79
Ultra-Micro Cuvettes (Hellma)
79
Standard, Semi-Micro, and Microcuvettes (Hellma)
80
Ultra-Micro Cuvettes (Hellma)
81
Index
83
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Molecular Devices SpectraMax M5e User Manual (20 pages)
Multi-Mode Microplate Readers
Brand:
Molecular Devices
| Category:
Laboratory Equipment
| Size: 3.21 MB
Table of Contents
Table of Contents
3
Safety Information
5
Warnings, Cautions, Notes, and Tips
5
Chapter 1: Introduction
9
Computer Integration
10
Supported Plates and Cuvettes
11
Shake
12
Temperature Regulation
12
Chapter 2: Setting up the Instrument
13
Connecting Instrument Cables
14
Chapter 3: Getting Started
15
Control Panel
16
Cuvette Chamber
18
Plate Drawer
19
Plate Adapter
20
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