User Manuals: Axon MultiClamp 700B Amplifier

User Manuals: Axon MultiClamp 700B Amplifier

Manuals and User Guides for Axon MultiClamp 700B Amplifier. We have 1 Axon MultiClamp 700B Amplifier manual available for free PDF download: Theory And Operation

Axon MultiClamp 700B Theory And Operation

Axon MultiClamp 700B Theory And Operation (172 pages)

COMPUTER-CONTROLLED MICROELECTRODE AMPLIFIER

Brand: Axon | Category: Amplifier | Size: 4.38 MB

Table of Contents

Table of Contents
5
Chapter 1 Introduction

11
Chapter 2 Installation and Basic Operation

13
- Installation
  13
  - Check List
    13
  - Installing Hardware
    14
  - Installing the Multiclamp 700B Commander
    14
- Functional Checkout
  16
Chapter 3 Tutorials

25
Chapter 4 Guide to Electrophysiological Recording

53
- General Advice
  54
  - Chamber Design
    54
  - Perfusion
    54
  - Mechanical Stability
    54
  - Optics
    55
  - Bath Electrode
    55
  - Interfacing a Computer
    56
  - Computer Noise
    57
- Patch Clamping
  57
- Sharp Microelectrode Recording
  69
  - Sharp Microelectrode or Patch Electrode
    70
  - Microelectrode Properties
    70
  - Filling Solutions
    74
  - Impaling Cells
    74
Chapter 5 Reference Section

77
- Audio Monitor
  78
- Bath Headstage and Electrodes
  82
  - Minimization
    82
  - Use of a Bath Headstage
    84
- Bridge Balance
  85
  - Bridge Balance in the Bath
    86
  - Bridge Balance in the Cell
    87
- Buzz
  88
- Capacitance Compensation
  88
- Capacitance Neutralization
  94
- Clear
  97
- Electrochemistry
  97
- External Command Inputs
  99
  - External Command Sensitivity
    99
  - Additivity of Commands
    100
  - Command Filter Frequency
    100
- Feedback Resistor
  101
  - V-Clamp Mode
    101
  - I-Clamp Mode
    103
- Filters
  103
  - Db Frequency
    104
- Types of Filters
  105
  - Bessel Filter
    105
  - Butterworth Filter
    105
  - Choosing the Cutoff Frequency
    105
  - High-Pass Filter
    107
  - Command Filter Frequency
    107
- Grounding and Hum
  107
- Headstage
  108
  - Voltage Clamp Circuit
    109
  - Current Clamp Circuit
    111
  - Mounting the Headstage
    112
  - Bath Connection
    112
  - Cleaning
    112
  - Static Precautions
    112
  - Acoustic Pick-Up
    112
- Help
  113
- Holders
  113
  - Holder Design
    113
  - Optional Ag/Agcl Pellets
    115
  - Holder Use
    115
  - Holder Maintenance
    117
  - Adapters
    117
- Input/Output Connections
  117
  - Front Panel
    117
  - Rear Panel
    119
- Leak Subtraction
  120
- Mode
  121
- Model Cell
  122
- Noise
  123
- Noise
  124
  - Measurement of Noise
    124
  - Sources of Noise
    124
- Oscilloscope Triggering
  126
- Output Zero
  127
- Overload
  128
- Polarity Conventions
  128
- Power Supply
  130
  - Supply Voltage Selection
    131
  - Changing the Fuse
    131
  - Glitches
    131
- Select Device
  132
- Series Resistance Compensation
  132
  - Introduction to R Compensation
    132
  - Is R Compensation Necessary
    134
  - Adjusting R Compensation
    135
    
    Theory of R Compensation
    139
    
    The 'Prediction' Control
    141
    
    Saturation Effects
    142
    
    Readjustment of Whole Cell Compensation with 'Prediction
    143
    
    The 'Correction' Control
    144
    
    Readjustment of Whole Cell Compensation with 'Correction
    145
    
    Setting 'Prediction' and 'Correction' Values
    145
    
    Oscillations
    145
    
    Limitations of R Compensation
    146
  - Softpanel Configuration
    147
  - Status
    148
  - Zap
    148
Chapter 6 Troubleshooting

149
Chapter 7 Specifications

151
References

157
Technical Assistance

159
Warranty and Repair Service

161
Declaration of Conformity

165
Important Safety Information

167
- Wichtige Informationen
  169
Index

171

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