User Manuals: Applied Biosystems 7300 Real Time PCR

Manuals and User Guides for Applied Biosystems 7300 Real Time PCR. We have 3 Applied Biosystems 7300 Real Time PCR manuals available for free PDF download: Installation And Maintenance Manual, Getting Started Manual

Applied Biosystems 7300 Installation And Maintenance Manual (174 pages)

Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 15.12 MB

7300/7500/7500 Fast System
5
Installation and Maintenance Workflow
5
Table of Contents
7
How to Use this Guide
11
Preface
11
How to Obtain more Information
12
How to Obtain Support
13
Safety and EMC Compliance Information
16
Safety Conventions Used in this Document
16
Symbols on Instruments
17
Safety Labels on Instruments
18
General Instrument Safety
20
Chemical Safety
21
Chemical Waste Safety
22
Electrical Safety
23
Biological Hazard Safety
24
Physical Hazard Safety
24
Safety and Electromagnetic Compatibility (EMC) Standards
25
Workstation Safety
25
Before Starting the Installation
27
Chapter 1 Before Starting the Installation

28
Chapter 2 Unpacking and Setting up the Instrument

34
Chapter 3 Installing the Software and Powering on the Instrument

50
Chapter 4 Performing the Instrument Function Test

56
Chapter 6 Performing the Pure Dye Calibration

94
Chapter 7 Verifying the Instrument Performance

114
Chapter 5 Performing the Verification Run

119
Appendix A Maintaining the Instrument

131
Appendix B Creating a Background Plate

151
- Creating a Background Plate
  151
- Overview
  151
Appendix C Creating a Custom Pure Dye Plate

153
- Overview
  153
- Determining Optimum Dye Concentration
  154
- Creating a Custom Pure Dye Plate
  156
- Adding the Custom Dye to the Software
  157
- Setting up the Computer
  159
Appendix D Setting up the Computer

160

Applied Biosystems 7300 Getting Started Manual (106 pages)

Fast Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 4.29 MB

Relative Quantification Experiment Workflow
3
Table of Contents
5
How to Use this Guide
7
Preface
7
How to Obtain more Information
9
How to Obtain Support
10
Introduction
11
About Relative Quantification
12
About the 7300/7500/7500 Fast System
12
Chapter 1 Introduction

12
- About RQ Experiments
  13
- Designing an RQ Experiment
  17
- Selecting the PCR Method
  18
Chapter 2 Designing an RQ Experiment

18
Chapter 3 Performing Reverse Transcription

28
- Converting Total RNA to Cdna
  29
Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System

31
Chapter 5 Generating Data from RQ Plates - 7500 Fast System

47
Chapter 6 Analyzing Data in an RQ Study

70
Appendix A Creating Detectors

89
Appendix B Example RQ Experiment

92
- References
  100
- Index
  102

Applied Biosystems 7300 Getting Started Manual (78 pages)

Fast Real-Time PCR System

Brand: Applied Biosystems | Category: Laboratory Equipment | Size: 5.59 MB

Plus/Minus Experiment Workflow
3
Table of Contents
5
How to Use this Guide
7
Preface
7
How to Obtain more Information
9
How to Obtain Support
10
Introduction
11
About Plus/Minus Assays Using an IPC
12
About the 7300/7500/7500 Fast System
12
Chapter 1 Introduction

12
Chapter 2 Designing a Plus/Minus Experiment

18
- Designing the Probe and Primers
  19
- Setting up the Reaction Plate
  21
- Preparing DNA
  22
Chapter 3 Setting up the Reaction Plate

22
Chapter 4 Performing the Plus/Minus Pre-Read Run

28
Chapter 5 Generating Amplification Data

38
- Performing the Amplification Run
  42
Chapter 6 Performing the Plus/Minus Post-Read Run

46
Appendix A Creating Detectors

56
Appendix B Viewing Amplification Data

58
- Viewing the Amplification Data
  60
Appendix C Example Plus/Minus Experiment

66
- References
  72
- Index
  74

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User Manuals: Applied Biosystems 7300 Real Time PCR

Applied Biosystems 7300 Installation And Maintenance Manual (174 pages)

Table of Contents

Chapter 1 Before Starting the Installation

Chapter 2 Unpacking and Setting up the Instrument

Chapter 3 Installing the Software and Powering on the Instrument

Chapter 4 Performing the Instrument Function Test

Chapter 6 Performing the Pure Dye Calibration

Chapter 7 Verifying the Instrument Performance

Chapter 5 Performing the Verification Run

Appendix A Maintaining the Instrument

Appendix B Creating a Background Plate

Appendix C Creating a Custom Pure Dye Plate

Appendix D Setting up the Computer

Applied Biosystems 7300 Getting Started Manual (106 pages)

Table of Contents

Chapter 1 Introduction

Chapter 2 Designing an RQ Experiment

Chapter 3 Performing Reverse Transcription

Chapter 4 Generating Data from RQ Plates - 7300 or Standard 7500 System

Chapter 5 Generating Data from RQ Plates - 7500 Fast System

Chapter 6 Analyzing Data in an RQ Study

Appendix A Creating Detectors

Appendix B Example RQ Experiment

Applied Biosystems 7300 Getting Started Manual (78 pages)

Table of Contents

Chapter 1 Introduction

Chapter 2 Designing a Plus/Minus Experiment

Chapter 3 Setting up the Reaction Plate

Chapter 4 Performing the Plus/Minus Pre-Read Run

Chapter 5 Generating Amplification Data

Chapter 6 Performing the Plus/Minus Post-Read Run

Appendix A Creating Detectors

Appendix B Viewing Amplification Data

Appendix C Example Plus/Minus Experiment

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