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SureCycler 8800
Agilent Technologies SureCycler 8800 Manuals
Manuals and User Guides for Agilent Technologies SureCycler 8800. We have
1
Agilent Technologies SureCycler 8800 manual available for free PDF download: Setup And User Manual
Agilent Technologies SureCycler 8800 Setup And User Manual (66 pages)
Brand:
Agilent Technologies
| Category:
Laboratory Equipment
| Size: 1.35 MB
Table of Contents
Table of Contents
5
1 Before You Begin
9
Materials Provided
10
Safety Precautions
10
Indicator LED Light
13
Overview
13
Surecycler 8800 Technical Support
14
2 Installation
15
Installation
16
Step 1. Select a Location for the Instrument
16
Step 2. Unpack the Cycler
16
Step 3. Install the Thermal Block on the Instrument Base
16
Step 4. Connect the Instrument to a Power Supply
17
Step 5. Connect Optional Devices
17
Step 6. Turn on the Instrument
17
Step 7. Connect the Cycler to a Network (Optional)
17
Thermal Block Exchange
18
To Remove the Thermal Block
18
To Install the Thermal Block
18
3 Getting Started
19
Sample Preparation and Loading to Prepare Samples to Load Samples
19
Agilent Plasticware and Consumables
21
Starting up the Software
22
Touchscreen Operation of the Software
22
Home Screen
22
User Accounts
24
Access Levels
24
Logging in to User Accounts
24
Managing User Accounts
26
Network Settings and Remote Access
29
To View or Edit the Cycler's IP Address and Name
29
To Access the Cycler from Another Cycler
30
To Access the Cycler from a PC
31
Cycler Settings
32
Hot Top Setting
32
Cycler Name
33
Time and Date
33
4 PCR Programs
35
Creating New PCR Programs
36
To Manually Create Programs
36
To Create a PCR Program Using the PCR Wizard
39
Tips for Creating PCR Programs for the 384-Well Surecycler 8800
42
Program Steps in the Commands List
43
Hot Start
43
Cycle Wizard
44
Start Cycle
45
Temperature Step
46
End Cycle
47
Gradient Step
47
Touchdown
49
Storage/Pause Step
50
Using a Preloaded PCR Program
51
To Access the Preloaded Programs
52
To Edit a Preloaded Program
52
Editing PCR Programs
53
To Edit Program Step Parameters
53
To Copy a PCR Step
54
To Add or Delete a PCR Step
54
To Delete a PCR Program
54
Running a PCR Program
55
To Run a PCR Program
55
To Stop or Pause a Program
55
Running an Incubation
56
To Set up and Run an Incubation
56
Viewing Reports
57
To View Reports
57
To Delete a Report
57
Troubleshooting
58
If PCR Yield Is Low When Running Small Reaction Volumes with the Standard Ramp Rate
58
If no Actual Temperature Is Displayed During the Run
58
If the Program Does Not Start
58
If Condensation Is Found in Tubes During a Run
59
If the Sealed Microplate Loses Sample Volume
59
If USB Devices Including Memory Stick and Keyboard Not Recognized
59
If Cycler Does Not Turn on
59
If Screen Is Blank and Unlit
60
If Screen Is Lit but Shows no Information or Has Missing Characters
60
5 Maintenance and Updates
61
Maintenance
62
Cleaning
62
Replacing a Fuse
62
Software Updates
63
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