Diascopic Illuminator; P2-Dbl Led Diascopic Illumination Base - Nikon SMZ25 Instruction Manual

8

Diascopic Illuminator

The primary diascopic illuminator for this microscope system consists of an illumination base equipped with an illuminating
optical system. A dark-field illuminator and polarizing illuminator are also available.

P2-DBL LED Diascopic Illumination Base

8.1
LED power source ON/OFF switch
This illumination base contains an optical system for diascopic illumination. Since the base supports OCC illumination,
high-contrast observation of transparent samples is enabled.
The control box supplies the power for illumination control if the control box is connected. Otherwise, a 12 V AC adapter must
be connected.
To turn on illumination, move the LED power ON/OFF switch on the front of the base to the "|" (ON) position. Use the LED
brightness control dial to adjust the brightness. Turn the LED brightness control dial clockwise to increase the brightness as
needed.
If the control box is connected, LED On/Off, brightness control, and light intensity monitoring are possible using the control
box by assigning the illumination control right to the control box. Use the brightness control selection switch on the base to
assign the control right to the base or to the control box.
 Brightness control selection switch Off: Assigns the control right to the base.
 Brightness control selection switch On: Assigns the control right to the control box.
The stage plate of the base is large (180 mm dia.) and made of transparent glass to enable more efficient work and easier
observation of samples in containers such as petri dishes. The base has three indents for holding the base: front of the
base's top board and on the right and left rear.
The stage plate can be replaced with a stage having an X/Y shift knob or a sliding stage. It is also possible to attach other
devices such as a fiber illuminator or diascopic polarizing device to expand the base's functions.
 OCC (oblique coherent contrast) illumination
The LED diascopic illumination base supports OCC illumination. OCC illumination is an oblique illumination method for
observing the phase objects, where a sample is illuminated with a coherent illuminating light obliquely by shielding a part
of the optical path using a shading plate. By this illumination method, a colorless transparent sample can be observed
with relief and contrast.
To adjust the OCC contrast, slide the OCC adjustment knob at the right front of the base back and forth. Adjust until the
optimum observation state is obtained in combination with the selected magnification and phase amount of the sample.
For ordinary bright-field microscopy under diascopic illumination, set the OCC adjustment control to either the farthest or
closest position. Sliding the control gradually forward or backward from that position will cause the OCC effect to increase.
Sliding the control by 46 mm will result in completely shielded status. Since adjustment in two directions is thus possible,
the direction of the OCC contrast can be reversed.
In addition, since the adjustment control has a scale, the control position can be reproduced.
Chapter 4
Functions and Operations of the Devices
Prism sheet
IN/OUT lever
OCC adjustment
knob
LED light control dial
P2-DBL LED Diascopic Illumination Base
LED brightness control
selection switch
INPUT
12VDC ,
3.5A
CTRL
OCC adjustment knob
54
CTRL
ON OFF
LED brightness
control terminal
AC adapter input terminal
Left side
1 . 6 - 2 x
0 . 5 - 1 x
Prism sheet
IN/OUT lever
Right side
I N
O U T