Summary of Contents for Life EVOS XL Core
- Page 1 user guide EVOS XL Core ® imaging system for Transmitted Light Applications Catalog Number AMEX1000 Publication Number MAN0007989 Revision A.0 For Research Use Only. Not for use in diagnostic procedures.
- Page 2 PURPOSE, OR NON-INFRINGEMENT. TO THE EXTENT ALLOWED BY LAW, IN NO EVENT SHALL LIFE TECHNOLOGIES AND/OR ITS AFFILIATE(S) BE LIABLE, WHETHER IN CONTRACT, TORT, WARRANTY, OR UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM THIS DOCUMENT, INCLUDING BUT NOT LIMITED TO THE USE THEREOF.
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Page 3: Table Of Contents
Contents About this Guide ............................. 5 1. Setup ........................6 Standard Items Included ..........................6 Operating Environment ..........................6 Power Supply ..............................7 Wireless Mouse and USB Flash Drive ......................7 USB Flash Drive ............................... 7 Optional: Attachable Mechanical Stage ......................8 Glass Stage Plate .............................. - Page 4 Appendix A: Troubleshooting..................20 Image Issues ..............................20 Mechanical Issues ............................21 Appendix B: Specifications ..................22 Appendix C: Installing the Attachable Mechanical Stage ..........23 Appendix D: Safety ...................... 25 Safety Conventions Used in this Document ....................26 Symbols on Instruments ..........................27 Safety Labels on Instruments ........................
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Page 5: About This Guide
Life Technologies instruments (see “Safety Symbols” on page 28). SDSs The Safety Data Sheets (SDSs) for any chemicals supplied by Life Technologies are available to you free 24 hours a day. For instructions on obtaining SDSs, see “Safety Data Sheets (SDS)” on page 36. -
Page 6: Setup
• Glass stage plate (packed in bubble wrap) • Dust cover * The attachable Mechanical Stage is an optional feature available separately from Life Technologies. For ordering information, contact your distributor or Technical Support (see page 36). Condenser shield, removable Operating Environment •... -
Page 7: Power Supply
Power Supply Turn the power switch to the “” (OFF) position before connecting the power adapter. Connect the power adapter to the power jack on the back of the instrument base and plug the cord into an outlet. IMPORTANT! Always use the correct power supply. The power adapter specifications appear on the serial number label and in “Appendix B: Specifications”... -
Page 8: Optional: Attachable Mechanical Stage
Optional : Attachable Mechanical Stage Follow the procedure in “Appendix C: Installing the Attachable Mechanical Stage” (page 23) to install the mechanical stage. Glass Stage Plate Unpack the glass stage plate and insert it into the stage opening (if a mechanical stage is installed, move it all the way back to expose the stage opening). -
Page 9: Installing Evos ® In A Cell Culture Hood
Installing EVOS ® in a Cell Culture Hood The small footprint of the EVOS ® XL Core Imaging System, its simple power connection, and its easily-viewed display make it quick to install and convenient to use in a cell culture hood. Dimensions ®... -
Page 10: Quick Reference: Instrument Contols
2. Quick Reference: Instrument Contols Note: See “Controls Glossary” on page 16 for more details about onscreen and manual controls. Manual controls Phase turret Coarse focus knob Freeze button Fine focus knob Save button Power switch Illumination wheel Power input jack Objective turret USB ports Mechanical stage... - Page 11 Onscreen controls Roll over the bottom of screen with the mouse to activate the onscreen controls. Illumination Slider Save button Freeze/Live toggle Settings button Note: Onscreen controls are only available when a mouse is installed.
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Page 12: Operation
3. Operation Basic Operation The EVOS ® XL Core Imaging System has both manual and onscreen operation controls. Manual controls include the illumination wheel, focus knobs, freeze and save buttons, objective turret, and the mechanical stage X-Y axis knobs. Onscreen controls appear at the bottom of the screen when the mouse rolls over the controls area (see page 11). - Page 13 Use the manual or the onscreen Freeze/Live toggle to acquire the image. Use the manual or the onscreen Save button to save the image. 10. If you use the onscreen button and Quick Save is not enabled, the virtual keyboard will pop up so you can enter a file name (Quick Save is automatically enabled with the manual save button).
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Page 14: Settings
Settings The Settings popup provides various controls to customize display performance and image file names and formats. The Settings button toggles to display or hide the Settings popup. Note: If the power is switched off before saving the settings, the system will revert to the most recently saved settings. -
Page 15: Software Updates
Software Updates Periodically, Life Technologies adds functionality and other improvements to the ® ® EVOS user interface. We recommend keeping your EVOS XL Core Imaging System up to date with the latest software. If you have any questions about software updates, contact your local EVOS ®... -
Page 16: Controls Glossary
4. Controls Glossary Note: This section describes all the onscreen and manual controls in detail. To see the most commonly used controls in context, see “Quick Reference: Instrument Controls” (page 10). Onscreen Controls This glossary is not alphabetized. Onscreen items are listed from left to right. Illumination slider The illumination slider controls the illumination intensity when the mouse is installed. -
Page 17: Manual Controls
Manual Controls This glossary is not alphabetized. Manual controls are listed in the order they are normally used, with a separate section for the optional Attachable Mechanical Stage (page 24). Power input jack Plug the power adapter into the power input jack. Power switch Set the power switch to “... -
Page 18: Care And Maintenance
• Do not exchange objectives between instruments unless you know that the components have been approved and recommended by Life Technologies. • After using, cover the instrument with the supplied dust cover. IMPORTANT! Never disassemble or service the instrument yourself. -
Page 19: Sterilization Procedures
Sterilization Procedures To sterilize the EVOS ® XL Core Imaging System, follow these procedures: Turn power OFF. Clean the LCD display. Use a soft, dry, lint-free cloth to wipe off any dust from the screen. b. Clean the LCD display with a non-alcohol based cleaner made for flat- panel displays. -
Page 20: Appendix A: Troubleshooting
Appendix A: Troubleshooting Image Issues Problem Possible Solutions • Set the phase turret to the BF position. Image is too dim, even at brightest • ® If a condenser slider from a different EVOS instrument has setting (particularly at higher been inserted into the condenser, remove the slider. -
Page 21: Mechanical Issues
Mechanical Issues Problem Possible Solutions • Entire mechanical stage shifts loosely Tighten screws under stage. See “Appendix B: Installing the over fixed stage Attachable Mechanical Stage” (page 23). • Use the correct vessel holder for the application (refer to the Vessel does not sit securely when ®... -
Page 22: Appendix B: Specifications
4×, 10×, 20×, 40×; plan LWD phase, infinity Objectives Optional 2× and 100× (no phase) available Objective turret 4-position; manual control Illumination LED (50,000+ hour life); adjustable intensity Contrast Transmitted light (brightfield and phase contrast) methods Condenser 3-position turret for brightfield and phase contrast; 60 mm working distance... -
Page 23: Appendix C: Installing The Attachable Mechanical Stage
Appendix C: Installing the Attachable Mechanical Stage The mechanical stage is an optional accessory. You may purchase it separately for ® use with the EVOS XL Core Imaging System. Refer to the product page www.lifetechnologies.com/evosxlcore or Technical Support for more information (see page 36). Installation/ Follow this procedure to attach the mechanical stage to the fixed stage. - Page 24 Stage Clip Use the stage clip to secure vessel holders or large samples (i.e., a T-75 flask or multi-well vessel) to the mechanical stage. When properly secured, the sample moves with the stage as you turn the X-axis and Y-axis knobs.
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Page 25: Appendix D: Safety
Appendix D: Safety This section includes the following topics: • Safety conventions used in this document • Symbols on instruments • Safety labels on instruments • General instrument safety • Chemical safety • Chemical waste safety • Electrical safety • Physical hazard safety •... -
Page 26: Safety Conventions Used In This Document
Safety Conventions Used in this Document Safety Alert Words Four safety alert words appear in Life Technologies user documentation at points in the document where you need to be aware of relevant hazards. Each alert word–IMPORTANT, CAUTION, WARNING, DANGER–implies a particular... -
Page 27: Symbols On Instruments
Symbols on Instruments Electrical Symbols The following table describes the electrical symbols that may be displayed on Life Technologies instruments. on Instruments Symbol Description Indicates the On position of the main power switch. Indicates the Off position of the main power switch. - Page 28 Safety Symbols The following table describes the safety symbols that may be displayed on Life Technologies instruments. Each symbol may appear by itself or in combination with text that explains the relevant hazard (see “Safety Labels on Instruments” on page 29). These safety symbols may also appear next to DANGERS, WARNINGS, and CAUTIONS that occur in the text of this and other product-support documents.
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Page 29: Safety Labels On Instruments
Safety Labels on Instruments The following CAUTION, WARNING, and DANGER statements may be displayed on Life Technologies instruments in combination with the safety symbols described in the preceding section. Hazard English Français Symbol CAUTION! Hazardous chemicals. Read the ATTENTION! Produits chimiques Safety Data Sheets (SDSs) before handling. -
Page 30: General Instrument Safety
General Instrument Safety WARNING! PHYSICAL INJURY HAZARD. Use this product only as specified in this document. Using this instrument in a manner not specified by Life Technologies may result in personal injury or damage to the instrument. Moving and Lifting... -
Page 31: Chemical Safety
Chemical Safety Chemical Hazard WARNING! CHEMICAL HAZARD. Before handling any chemicals, refer Warning to the Safety Data Sheet (SDS) provided by the manufacturer, and observe all relevant precautions. WARNING! CHEMICAL HAZARD. All chemicals in the instrument, including liquid in the lines, are potentially hazardous. Always determine what chemicals have been used in the instrument before changing reagents or instrument components. -
Page 32: Chemical Waste Safety
Chemical Waste Safety Chemical Waste CAUTION! HAZARDOUS WASTE. Refer to Safety Data Sheets (SDSs) Hazard and local regulations for handling and disposal. Chemical Waste To minimize the hazards of chemical waste: Safety Guidelines • Read and understand the Safety Data Sheets (SDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. -
Page 33: Electrical Safety
Electrical Safety DANGER! ELECTRICAL SHOCK HAZARD. Severe electrical shock can ® result from operating the EVOS XL Core Imaging System without its instrument panels in place. Do not remove instrument panels. High‐voltage contacts are exposed when instrument panels are removed from the instrument. -
Page 34: Physical Hazard Safety
Physical Hazard Safety Moving Parts WARNING! PHYSICAL INJURY HAZARD. Moving parts can crush and cut. Keep hands clear of moving parts while operating the instrument. Disconnect power before servicing the instrument. Biological Hazard Safety WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, and blood of humans and other animals have the potential to transmit infectious diseases. -
Page 35: Safety And Electromagnetic Compatibility (Emc) Standards
The protection provided by the instrument may be impaired if the instrument is used in a manner not specified by Life Technologies. Australian EMC The C-Tick Mark indicates conformity with Australian and New Zealand standards for electromagnetic compatibility. -
Page 36: Documentation And Support
Technologies contact the chemical manufacturer. Limited Product Life Technologies Corporation and/or its affiliate(s) warrant their products as set forth in the Life Technologies’ General Terms and Conditions of Sale found on Warranty Life Technologies’ website at www.lifetechnologies.com/termsandconditions. If you have any questions, please contact Life Technologies at... - Page 37 For support visit lifetechnologies.com/support or email techsupport@lifetech.com lifetechnologies.com 10 October 2014...
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