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Photometrics Evolve 512 User Manual

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Summary of Contents for Photometrics Evolve 512

  • Page 2 User Manual for Evolve 512...
  • Page 3 Other brand and product names are the trademarks or registered trademarks of their respective owners and manufacturers. The information in this publication is believed to be accurate as of the publication release date. However, Photometrics does not assume any responsibility for any consequences including any damages resulting from the use thereof. The information contained herein is subject to change without notice.
  • Page 4 Customer Service If you have any questions about your camera system, please contact Photometrics Customer Service. When you call, please have your Photometrics part number or equipment serial numbers available. BENELUX FRANCE Photometrics Roper Scientific, BV Roper Scientific, SARL 3440 East Britannia Drive Ir.

  • Page 5: Limited Warranty

    Photometrics warrants this product against substantial defects in materials and / or workmanship for a period of up to one (1) year after shipment. During this period, Photometrics will repair the product or, at its sole option, repair or replace any defective part without charge to you.
  • Page 6 Photometrics. 2. You must notify the Photometrics factory service center within (30) days after you have taken delivery of a product or part that you believe to be defective. With the exception of customers who claim a “technical issue”...
  • Page 7 Evolve 512 Users Manual...
  • Page 8 Evolve 512 Users Manual...

  • Page 9: Table Of Contents

      Installing the FireWire Interface Card .................. 7   Connecting Your Evolve 512 Camera ..................7     To connect your Evolve 512 camera ..................7 Chapter 3 Operating Features   Introduction ..........................9     On-Chip Multiplication Gain ....................9  ...
  • Page 10 Figure 14. Bulb Mode Timing Diagram ................16   Figure 15. Benefit of Varibit ....................18   Figure 16. DAC Setting vs. Charge Multiplication Gain for Evolve 512 ......20   Figure 17. RapidCal Status ..................... 21   Figure 18. BERT Off vs BERT On ..................22  ...
  • Page 11 Tables Table 1. Evolve 512 Readout Speeds and Typical Conversion Gains ......25   Table 2. Application Type and Evolve 512 Setup Parameters ......... 26   Table 3. CCD Specifications ....................30   Table 4. Input/Output Definitions ..................32  ...
  • Page 12 THIS PAGE INTENTIONALLY LEFT BLANK Evolve 512 Users Manual...

  • Page 13: Chapter 1 Overview

    Chapter 1 Overview Introduction The Photometrics® Evolve camera employs an advanced CCD technology called "impact ionization" to achieve charge multiplication gain above the read noise. As a result, the cameras offer unparalleled sensitivity even at high frame rates. Evolve cameras are most suited for high speed and/or low-light level imaging applications such as single molecule fluorescence, ion imaging, and adaptive optics.

  • Page 14: System Components

    Figure 1. Comparison of Traditional CCD and Evolve CCD Array Structures The Evolve 512 also offers a non-multiplication readout similar to the one available in traditional CCD cameras. This makes these more versatile cameras that can be used for standard imaging applications requiring higher dynamic range.

  • Page 15: About This Manual

    About This Manual The Evolve 512 User Manual is divided into five chapters. It is suggested that you read the entire manual before operating the camera in order to ensure proper use. The chapter contents are briefly described below. Note: The information in these chapters applies only to the Evolve 512 and is currently not applicable to any other Photometric camera.

  • Page 16: Environmental Requirements

    The Evolve 512 camera can be mounted to a tripod using the tripod mounting attachment located on the sides of the camera.

  • Page 17: Chapter 2 System Installation

    Carefully review the Precautions section in the previous chapter before performing any of the procedures outlined here. Again, use only an Evolve data cable and an Evolve FireWire interface card with your Evolve 512 camera. Using a different cable or interface card may result in permanent damage to your system.

  • Page 18: Host Computer Requirements

    Note: The above requirements are the minimum for operating an Evolve camera. A faster computer with larger memory (RAM) will greatly enhance the software performance during live mode operations. If you are a Mac® user, the host computer for your Evolve 512 camera must have the following: ...

  • Page 19: Installing The Firewire Interface Card

    Connector Pinout section in Chapter 5 for details)  Power switch: Turns the camera on and off. The following connector is located on the power supply of the Evolve 512 camera:  I/O connector: Hirose HR10A-10R-10S, 10-pin connector for input/output...

  • Page 20: Figure 4. Evolve 512 Side Panel

    2. Connect the right-angled end of the DATA cable to the FireWire port (indicated with a red arrow in next graphic) located on the side of the camera. Figure 4. Evolve 512 Side Panel 3. Verify that the power switch on the side of the camera is in the off position.

  • Page 21: Chapter 3 Operating Features

    Chapter 3 Operating Features Introduction The features described in this section are identical in all Evolve 512 cameras unless otherwise stated. On-Chip Multiplication Gain As explained previously, the Evolve 512 uses a unique CCD capable of multiplying the charge (electrons) generated in the pixels. When the multiplication is sufficiently high, it is possible to see extremely low-light events.

  • Page 22: Non-Overlap Mode

    “Pre-exposure” with clear count of “1”(or more). Figure. 6 shows these settings on a software dialogue box. Note: Since the software you are using may show the settings differently, you should refer to the software documentation for accurate information. Evolve 512 Users Manual...

  • Page 23: Example: Non-Overlap Mode

    Select these parameters to operate your Evolve 512 camera in Non-Overlap mode. Figure 6. Required Settings for Non-Overlap Mode Operation Example: Non-Overlap Mode Operation in Non-Overlap mode is illustrated in the timing diagram below. In this example, the exposure time is 10 ms and the readout time is 29.5 ms. The total time to take 3 frames is 118.5 ms (3 ...

  • Page 24: Overlap Mode (Simultaneous Exposure-Readout)

    This mode of operation allows you to continuously image a specimen to obtain better kinetic information about a process. Figure 8 shows the required Evolve 512 settings for overlap mode. Note that the clearing mode is set to "Pre-sequence" (as opposed to "Pre-exposure" in Non-Overlap mode).

  • Page 25: Figure 9. Timing Diagram For Overlap Mode When Exposure Time < Readout Time

    = readout time for one frame From the timing diagram, you can see that because the exposure time is greater than the readout time, all frames are precisely exposed for the duration entered into the software and have similar intensities. Evolve 512 Users Manual...

  • Page 26: Triggered Operation

    Photometrics to access primary signals such as "Trigger input," "Expose out," "Frame readout," and "Shutter out." In the default mode, the camera triggers on the rising edge of a TTL signal. Evolve 512 cameras support the trigger modes described in the next sections. Trigger-first Mode In this mode, the camera requires only one trigger to acquire a sequence of frames.

  • Page 27: Strobe Mode

    Read Out is completed. It goes low when the Trigger signal is accepted and exposure is started. The shaded areas denote the idle time between exposures. Strobe mode only operates in Non-Overlap mode. Figure 13. Strobe Mode Timing Diagram Evolve 512 Users Manual...

  • Page 28: Bulb Mode

    For more information, refer to the "On-Chip Multiplication Gain" technical note. This technical note can be accessed by going to the Photometrics web site at www.photomet.com, clicking on "Library", clicking on "Technical Notes", and selecting the title from the list of notes.

  • Page 29: Readout Speed (Typical)

    Readout Speed (Typical) Evolve 512 cameras are implemented with 10 MHz, 5MHz and 1.25 MHz digitization speeds (10 million, 5 million and 1.25 million pixels per second, respectively). At 10 MHz it gives approximately 31 frames per second (fps), whereas 1.25 MHz operation offers better image quality.

  • Page 30: Evolve 512 Features

    Evolve 512 Features Photometrics has developed several new industry-leading camera capabilities to streamline user workflow and enable researchers to concentrate directly on the image data that are important to their studies. These advanced functions enhance the quantitative nature of the camera while simultaneously allowing researchers to home in on the data they are looking for.

  • Page 31: Quant-View

    65,535 gray levels per pixel can be sent over the bus. However, for the CCD97 used in the Evolve 512 camera, the total number of electrons possible in a single pixel can easily reach 200,000. Thus, if the camera was set at 1 electron per gray level, then the pixel would come across as saturated (it would max out at the 65,535 value).

  • Page 32: Rapid-Cal

    Rapid-Cal Calibration The Evolve 512 has incorporated a calibration routine which allows the camera to adjust voltages of the ADC offsets such that the electron multiplication gain input given to the camera reflects the actual gain provided by the device. Settings 1 to 1000 on the electron multiplication gain slider are mapped linearly to provide the actual multiplication gain requested by the user.

  • Page 33: Background Event Reduction Technology

    These are often referred to as “speckles” in the image. Photometrics has developed this real-time feature to help users identify such events and correct for them, in their images in real-time.

  • Page 34: Black-Lock / Top-Lock

    This advanced feature allows users to define an effective floor for an image at a level which they select. In the case of the Evolve 512, many users operate the camera in EM gain mode and apply EM gain at high levels. In such scenarios, EMCCD cameras can end up having multiple ADUs per electron.

  • Page 35: Dual-Readout Port Operation

    Dual-Readout Port Operation The Evolve 512 is configured with software-selectable dual-readout amplifiers, also referred to as "ports", shown in the next figure. The two amplifiers are listed below. Port #1: On-chip Multiplication amplifier Port #2: Traditional or non-multiplication amplifier Figure 19.

  • Page 36: Figure 20. Software Selection Of Readout Port

    Port #1. Some software packages do a "horizontal-flip" in the software when Port #2 is selected to ensure the correct orientation for all ports. Please check your software documentation. Evolve 512 Users Manual...

  • Page 37: Conversion Gain

    1, 2, 4 and 8 in the serial (horizontal) direction and binning in the parallel (vertical) direction, up to 256. Note: Due to the small array size in the Evolve 512 edge artifacts may be present at higher binning. Evolve 512 Users Manual...

  • Page 38: Evolve 512 Application Examples

    Application Settings Summary The table below summarizes typical Evolve 512 settings for two types of applications and is provided as a setup aid. Actual settings will vary based on the exact nature of your experiment design. Readout...

  • Page 39: Chapter 4 Troubleshooting

    Windows 2000/XP:  Check to make sure that the new interface card is inserted in an expansion slot according to your computer manufacturer’s instructions and that the Evolve 512 system’s CD-ROM disc is in the host computer’s CD drive. ...

  • Page 40: Images Not Displayed

    Confirm that the camera is operational by taking an image with a standard C-mount lens attached to your Evolve 512. Using normal room lighting, place the camera on a table about 3 meters away from an object and acquire an image.

  • Page 41: Chapter 5 Basic Specifications

    Chapter 5 Basic Specifications Evolve 512 Front/Side Views Figure 21. Evolve 512 Front View Figure 22. Evolve 512 Side View Evolve 512 Users Manual...

  • Page 42: Camera Weight

    Digitalization (Readout) 10 MHz, 5 MHz, 1.25 MHz Rate Table 3. CCD Specifications Connectors Figure 23. Evolve 512 Side Panel The following connectors are located on the side of the camera.  connector: Type 1, 6 pin IEEE-1394a (FireWire). ...

  • Page 43: Power Connector Pinout

    13. +16V 14. FPGA-GND 15. SPARE IO-2 Figure 24. Power Connector Pinout 16. EXPOSE 17. TX-IO 18. EOF 19. GND 20. TEC GND 21. TEC CONTROL 22. TEC GND 23. + 37V 24. GND 25. GND Evolve 512 Users Manual...

  • Page 44: Figure 25. Input/Output Connector

    A high level on this output indicates that the camera power is switched on (+5 V = on, 0 V = off). System digital ground. Any external circuitry intended to interface with the trigger control signals must reference this ground connection. Table 4. Input/Output Definitions Evolve 512 Users Manual...

  • Page 45: Power Supply Specifications

    Power Supply Weight: 5 lb (2.267 kg) Supply Cable Length: 6 ft. / 1.828 m Note: CE certification applies to the Evolve 512 only when the camera system is operated with a CE- approved power supply Figure 26. Power Supply Front...

  • Page 46: Appendix

    17. Turn on camera power and continue setup per quick-start guide. NOTE: DO NOT turn-on camera power without liquid circulating! 18. Open imaging software to set target temperature (-100C.) 19. Allow approximately 30 minutes to stabilize at target temperature. Figure 27. Liquid Cooled Evolve Setup Diagram Evolve 512 Users Manual...

  • Page 47: Index

    Trigger modes bulb, 16 Liquid Cooled, 34 strobe, 15 M  trigger-first, 14 Microscopes, 4 Trigger-first mode, 14 Multiplication Tripods, 4 gain, 1 W  port, 16 register, 1 Warranty, iii Your responsibility, iv N  Non-overlap readout mode, 10 Evolve 512 Users Manual...
  • Page 48 57-519-001 Rev A3...

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