Page 1 ICS-2100 Ion Chromatography System Operator’s Manual Document No. 065291 Revision 01 March 2009...
Page 2 ©2009 by Dionex Corporation All rights reserved worldwide. Printed in the United States of America. This publication is protected by federal copyright law. No part of this publication may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or transmitted into any human or computer language, in any form or by any means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties without the express written permission of Dionex Corporation, 1228 Titan Way,...
Page 3: Table Of Contents
Contents 1 • Introduction ..........1 Introduction to Ion Chromatography (IC) .
Page 4 ICS-2100 Ion Chromatography System 2.4.2 Eluent Valve ........34 2.4.3 Pump .
Page 5 Contents Prepare Samples ......... . 60 3.9.1 Collecting and Storing Samples .
Page 6 ICS-2100 Ion Chromatography System 4.10 Nonreproducible Peak Height or Retention Time ....94 4.11 Abnormal Retention Time or Selectivity ......94 4.12 No Cell Response .
Page 7 Contents 5.12 Replacing the Column Heater ....... 131 5.13 Replacing the Column Heater Heat Exchanger ....134 5.14 Replacing the Eluent Valve .
Page 8 ICS-2100 Ion Chromatography System 5.23.2 Removing the CR-TC and Reinstalling it in the New EGC Holder ......188 5.23.3 Removing the EPM and Reinstalling it in the New EGC Holder .
Page 9 Contents B • Touch Screen Operation ......207 Using the Touch Screen ........207 Using the Touch Screen with Chromeleon .
Page 10 ICS-2100 Ion Chromatography System B.8.4 Monitoring the EluGen Cartridge Life ....228 B.8.5 Controlling the CR-TC Power ......229 Suppressor Page .
Page 11 Contents How do I adjust retention times? ......255 When should I remake standards? ......255 When should I replace the eluent generator cartridge? .
Page 12 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 13: Introduction
1 • Introduction Introduction to Ion Chromatography (IC) The Dionex ICS-2100 Ion Chromatography System (ICS-2100) performs ion analyses using suppressed or non-suppressed conductivity detection. An ion chromatography system typically consists of a liquid eluent, a high-pressure pump, a sample injector, a guard and separator column, a chemical suppressor, a conductivity cell, and a data collection system.
Page 14 ICS-2100 Ion Chromatography System A typical IC analysis consists of six stages (see Figure 1-1). 6. Data Analysis Conductivity 5. Detection Cell Suppressor 4. Suppression Eluent 1. Eluent Separator Delivery Column 3. Separation Pump Guard Column Injection Valve Sample 2. Sample Injection Sample Loop Figure 1-1.
Page 15 1 • Introduction • The pump pushes the eluent and sample through the guard and separator columns (chemically-inert tubes packed with a polymeric resin). The guard column removes contaminants that might poison the separator column. 3. Separation • As the eluent and sample are pumped through the separator column, the sample ions are separated.
Page 16: Overview Of The Ics-2100
ICS-2100 Ion Chromatography System Overview of the ICS-2100 The ICS-2100 is an integrated ion chromatography system containing an eluent generator, pump, injection valve, column heater, and conductivity detector. Other system components, including a guard column, separator column, and suppressor vary, depending on the analyses to be performed. The ICS-2100 can be configured with a vacuum degas assembly for online eluent degassing.
Page 17: About This Manual
1 • Introduction About This Manual The electronic version (i.e., PDF file) of this operator’s manual contains numerous hypertext links that can take you to other locations within the file. These links include: • Table of contents entries • Index entries •...
Page 18: Safety Messages And Notes
ICS-2100 Ion Chromatography System Appendix E Provides answers to frequently asked questions about ICS- 2100 operation. Appendix F Defines terms commonly used in ion analysis. Glossary 1.3.1 Safety Messages and Notes This manual contains warnings and precautionary statements that, when properly followed, can prevent personal injury and/or damage to the instrument.
Page 19 1 • Introduction Warnhinweise in Deutsch Bedeutet unmittelbare Gefahr. Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen. Bedeutet eine mögliche Gefährdung. Mißachtung kann zum Tod oder schwerwiegenden Verletzungen führen. Bedeutet eine mögliche Gefährdung. Mißachtung kann zu kleineren oder mittelschweren Verletzungen führen. Wird auch verwendet, wenn eine Situation zu schweren Schäden am Gerät führen kann, jedoch keine Verletzungsgefahr besteht.
Page 20: Safety And Regulatory Information
ICS-2100 Ion Chromatography System Safety and Regulatory Information The ICS-2100 was manufactured by Dionex Corporation at the following location: 527 Lakeside Drive, Sunnyvale, CA 94088-3603 U.S.A. The ICS-2100 is designed for IC (ion chromatography) applications and should not be used for any other purpose.
Page 21 1 • Introduction Power supply is on Power supply is off Hot surface Indicates a potential hazard. Refer to the operator’s manual for an explanation of the hazard and how to proceed. Doc. 065291-01 3/09...
Page 22 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 23: Features
2 • Features This chapter describes key ICS-2100 features and introduces the Chromeleon user interface. Operating Features 2.1.1 Front Panel Figure 2-1 illustrates the front panel of the ICS-2100. Injection Port The sample to be analyzed can be injected manually into the injection port, using a syringe.
Page 24 ICS-2100 Ion Chromatography System Summary of Touch Screen Operation Status Field Edit Field Command Button (selected) Command Button (unselected) Figure 2-2. ICS-2100 Touch Screen Home Page NOTE To adjust the screen contrast, open the front door and adjust the knurled knob under the screen (see Figure 2-8).
Page 25 2 • Features • Selecting a command button changes the button’s appearance to white text on a dark backgound (for example, • Touching an edit field (for example, ) opens a page with a number keypad (see Figure 2-3). Use the keypad to enter the desired numerical value for the field and then touch the ENTER button.
Page 26 ICS-2100 Ion Chromatography System • Touching the page name (for example, ) in the bottom right corner opens a menu of pages (see Figure 2-4). Touch a page name to display the page. Figure 2-4. ICS-2100 Touch Screen Menu of Pages •...
Page 27 2 • Features page (see Figure 2-5). See Appendix B for details SUPPRESSOR about each touch screen page. Touch to select a different option. Touch to return to the Home page. Figure 2-5. Suppressor Page • Touching returns you to the page.
Page 28: Top Cover
ICS-2100 Ion Chromatography System 2.1.2 Top Cover Figure 2-7 illustrates the top cover of the ICS-2100. EluGen Cartridge and Reservoir EluGen Storage Cartridge The top cover holds one or two and Holder ® EluGen cartridges and up to Second two 2-L plastic reservoirs EluGen (P/N 044129) or one 4-L plastic Cartridge or...
Page 29 2 • Features EluGen cartridge holder, is connected to the connector. However, EGC-2 the connections can be reversed, if desired. EGC Service Area The EGC service area holds the cartridge during installation and replacement. For details, refer to the ICS-2100 installation instructions. Doc.
Page 30: Component Panel
ICS-2100 Ion Chromatography System 2.1.3 Component Panel Figure 2-8 shows the user-accessible components installed on the component panel behind the ICS-2100 front door. Pressure Transducer Leak Sensor Pump Heads Injection Valve Auxiliary Valve (Optional) DS6 Conductivity Cell Suppressor Column Heater and Columns Eluent Valve Mounting Brackets for...
Page 31 2 • Features Pressure Transducer The pressure transducer measures the system backpressure. Leak Sensor The leak sensor is installed in the drip tray at the bottom of the component panel. If liquid accumulates in the tray, an error message is logged in the Chromeleon Audit Trail and displayed on the LCD touch screen.
Page 32 ICS-2100 Ion Chromatography System Suppressor The suppressor reduces the eluent conductivity and enhances the conductivity of the sample ions, thereby increasing detection sensitivity. ® ® Either an AES Atlas Electrolytic Suppressor, SRS Self-Regenerating ™ ™ Suppressor, or MMS MicroMembrane Suppressor can be used with the ICS-2100.
Page 33: Rear Panel
2 • Features 2.1.4 Rear Panel Figure 2-9 illustrates the ICS-2100 rear panel. Tubing Clips Analog Output Connector Power Switch Connectors TTL and Relay Main Power Connector Strip Receptacle Waste Lines Figure 2-9. ICS-2100 Rear Panel Analog Output Connector The analog output connector outputs conductivity data (as a 0 to 1 V signal) to an integrator or recording device.
Page 34 ICS-2100 Ion Chromatography System TTL and Relay Connector The TTL and Relay connector strip provides two TTL outputs, two relay outputs, and four TTL inputs. The outputs can be used to control functions in other TTL- or relay-controllable devices. The inputs can be used to switch the injection valve position, turn on the pump, perform an autozero command, and send an event mark to the analog output.
Page 35: Flow Schematics
2 • Features Flow Schematics • Figure 2-10 illustrates the liquid flow path when the components required for producing KOH, LiOH, MSA, or NaOH eluent are installed. The required components include the corresponding type of EluGen cartridge and a CR- •...
Page 36 ICS-2100 Ion Chromatography System To the pump Degas In EGC Vent EGC II KOH, LiOH, MSA, or NaOH Deionized Water Inlet Outlet Degas CR-TC From the pulse damper Degas In To the Column inject valve Heater port P(2) Degas Out Conductivity Cell Suppressor...
Page 37 2 • Features Flow Description for KOH, LiOH, MSA, or NaOH Eluent Generation Refer to Figure 2-10 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump .
Page 38 ICS-2100 Ion Chromatography System To the pump Degas In EGC Vent EGC II Deionized Water Inlet Outlet Eluent Out Regen Out Eluent In Degas Regen In Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve Press.
Page 39 2 • Features Flow Description for Carbonate/Bicarbonate Eluent Generation Refer to Figure 2-11 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump . The water is then pushed through the pressure transducer , which measures the system pressure.
Page 40 ICS-2100 Ion Chromatography System To the pump Degas In EGC Vent EGC II Deionized Water Inlet Outlet Degas Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve Press. Xducer. To waste Pump Pump Head Head To Gas...
Page 41 2 • Features Flow Description for Carbonate Eluent Generation Refer to Figure 2-12 for the flow path number locations. • Deionized water from the reservoir flows first through the pump degas assembly and then through the eluent valve to the pump . The water is then pushed through the pressure transducer , which measures the system pressure.
Page 42: Chromeleon And Chromeleon Xpress
ICS-2100 Ion Chromatography System Chromeleon and Chromeleon Xpress The ICS-2100 is controlled by a PC configured with Chromeleon Chromatography Management System or Chromeleon Xpress. Chromeleon Chromatography Management System provides complete instrument control, data acquisition, and data management. Chromeleon Xpress provides real-time control and monitoring of Dionex chromatography instruments, but does not include data management capabilities.
Page 43: Software Control Modes
2 • Features • A Sequence Control panel lets you define and run sequences (groups of sample injections to be analyzed in the order in which they are listed). • A Status panel shows the overall system status. • An Autosampler panel (included if the ICS-2100 is connected to an AS, AS-DV, or AS-HV Autosampler) provides access to autosampler functions.
Page 44: System Component Details
ICS-2100 Ion Chromatography System System Component Details This section provides details about ICS-2100 system components, including the vacuum degas assembly, pump, eluent generator, injection valve, column heater, suppressor, auxiliary power supply, and conductivity cell. 2.4.1 Vacuum Degas Assembly (Optional) The vacuum degas assembly provides online eluent degassing at the time and duration specified by the user.
Page 45 2 • Features Select the Options tab (see Figure 2-14). Figure 2-14. Server Configuration Properties: Degas Options Degas Options • Always Off : The degas pump is always off. • : The degas pump is always on. This setting is for test Always On purposes by a Dionex Service Representative.
Page 46: Eluent Valve
ICS-2100 Ion Chromatography System 2.4.2 Eluent Valve The eluent valve controls the flow from the eluent reservoir. The valve opens automatically when the pump is started and closes when the pump is turned off. The valve can also be opened and closed manually from the Chromeleon Control panel (see Section...
Page 47 2 • Features Outlet Check Valve Secondary Primary Pump Head Pump Head Waste Valve Priming Valve Inlet Check Valve Pressure Transducer Figure 2-16. ICS-2100 Pump Components Secondary Pump Head The secondary pump head delivers eluent to the remainder of the chromatography system (the injection valve, column, and detector).
Page 48 ICS-2100 Ion Chromatography System Pressure Transducer Flow exiting the secondary pump head is directed to the pressure transducer (see Figure 2-16), which measures the system pressure. Pressure readings indicate that the pumping system is delivering smooth, accurate flow. Pressure readings can be monitored from the Chromeleon Control panel or the touch screen page.
Page 49: Eluent Generator
2 • Features 2.4.4 Eluent Generator The eluent generator produces high-purity eluents online, using only deionized water as the carrier. The eluent generator consists of an eluent generator cartridge (EGC) that generates the eluent, and a high-pressure degas tubing assembly that removes electrolysis gases created during eluent generation.
Page 50 ICS-2100 Ion Chromatography System You can select the concentration of eluent to be generated from either the Chromeleon Control panel or from the touch screen page (see HOME Section B.4). The allowable eluent concentration depends on several factors: the flow rate, suppressor type, EluGen cartridge type, and cartridge configuration.
Page 51 2 • Features details, refer to the EluGen cartridge manual. Cartridge manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). EluGen Eluent Concentration Range Comment Cartridges /EPM 0.1 to 15 mM at 0.1 to 1.0 mL/min The total of the eluent flow concentrations from Electrolytic...
Page 52 ICS-2100 Ion Chromatography System Tubing and fittings for plumbing the cartridge, degas assembly, CR-TC, and EPM are included with the holder. For connection instructions, refer to the ICS-2100 installation instructions. Backpressure Coil (Optional) The EluGen cartridge requires at least 14 MPa (2000 psi) of system backpressure for removal of electrolysis gas from the eluent produced by the cartridge.
Page 53 2 • Features EPM Electrolytic pH Modifier and EGC CO Mixer The EGC II K EluGen Cartridge can be used with an EluGen Cartridge EPM Electrolytic pH Modifier (P/N 063175) (see Figure 2-17) and an EGC CO Mixer (P/N 061686) to produce a carbonate/ bicarbonate mixture.
Page 54: Auxiliary Power Supply (Optional)
ICS-2100 Ion Chromatography System the EPM to achieve the desired 3.50 mM K /1.00 mM KHCO mixture. 2.4.5 Auxiliary Power Supply (Optional) The EGC-2 power supply can be configured as an auxiliary power supply for the operation of an electrolytic device such as a water purifier. The power supply operates in constant current mode and can be configured from 0 to 200 mA, with a maximum voltage of 35 V.
Page 55 2 • Features syringe or automated sampler line (if installed), through the valve, and into the sample loop. Excess sample flows out to waste. • In the Inject position, sample is swept to the column for analysis. Eluent flows from the pump, through the sample loop, and on to the column, carrying the contents of the sample loop with it.
Page 56: Auxiliary Valve (Optional)
ICS-2100 Ion Chromatography System 2.4.7 Auxiliary Valve (Optional) The auxiliary valve is a high-pressure Rheodyne valve. The electrically- activated, 2-position PEEK valve is offered in both 6-port and 10-port models (6-Port Valve Kit, P/N 069472; 10-Port Valve Kit, P/N 069473). The auxiliary valve enables a variety of sample preparation activities, including: •...
Page 57: Column Heater
2 • Features 2.4.8 Column Heater The column heater provides temperature control for the separator and guard column. The heater temperature can be set to between 30 °C and 60 °C. However, the set temperature must be at least 5 °C above the ambient temperature.
Page 58: Suppressor
ICS-2100 Ion Chromatography System 2.4.9 Suppressor The suppressor reduces the eluent conductivity and enhances the conductivity of the sample ions, thereby increasing detection sensitivity. Either an Atlas Electrolytic Suppressor, Self-Regenerating Suppressor, or MicroMembrane Suppressor can be used with the ICS-2100. For details about any of the suppressors or for information about selecting a suppressor for your application, refer to the suppressor manuals.
Page 59 2 • Features DS6 Heated Conductivity Cell Components The cell front cover provides fittings for CELL IN CELL OUT connecting the cell to the suppressor (see Figure 2-8). The remaining cell components are mounted behind the component panel. To replace, the cell remove the screws on the cell front cover and pull the entire cell assembly out through the component panel.
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Page 61: Operation And Maintenance
3 • Operation and Maintenance This chapter describes routine operating and maintenance procedures for the ICS- 2100. The ICS-2100 is designed for IC (ion chromatography) applications and should not be used for any other purpose. Operation of the ICS-2100 in a manner not specified by Dionex may result in personal injury.
Page 62 ICS-2100 Ion Chromatography System Sample Processing Overview Samples can be run manually (one at a time), or they can be grouped and run automatically in batches. Figure 3-2 shows the typical steps for manual and batch sample processing. Manual Sample Manual Sample Batch Sample Processing...
Page 63: Turning On The System Power
3 • Operation and Maintenance Turning On the System Power Press the power switch on the ICS- 2100 rear panel (see Figure 3-3) to turn on the system power. Table 3-4 shows the ICS-2100 conditions at power-up. Power Switch Also turn on the power to the computer and the autosampler (if used).
Page 64: Connecting To Chromeleon
ICS-2100 Ion Chromatography System Connecting to Chromeleon NOTE If you are beginning operation of an ICS-2100 that has not been configured in a timebase, refer to the ICS-2100 installation instructions Chromeleon setup instructions. 1. Turn on the PC. 2. Start the Chromeleon Server, if it is not already running. a.
Page 65 3 • Operation and Maintenance Figure 3-4. ICS-2100 Control Panel on the Chromeleon Panel Tabset Doc. 065291-01 3/09...
Page 66: Set Up The Eluent Reservoir
ICS-2100 Ion Chromatography System Set Up the Eluent Reservoir The ICS-2100 does not require pressurized reservoirs. However, if eluent is manually degassed or is sensitive to contamination, Dionex recommends pressurizing the reservoir with helium or nitrogen. The air regulator accessory bracket and other items needed for pressurizing the eluent reservoir must be ordered separately (P/N 060054).
Page 67 3 • Operation and Maintenance In order for the level displayed in the Eluent Bottle box and gauge to be accurate, you must enter the level when the reservoir is filled. The ICS-2100 does not automatically detect when the reservoir is filled, nor when it is empty.
Page 68: Connect The Reservoir
ICS-2100 Ion Chromatography System Section B.6 for details about the STATUS PAGE Figure 3-7. Status Page 3.4.4 Connect the Reservoir If it is not already connected, connect the ELUENT BOTTLE line from the reservoir ELUENT cap to the line, ELUENT IN BOTTLE which extends from the plumbing and cable chase on...
Page 69: Check All Connections
3 • Operation and Maintenance Check All Connections 1. Make sure the eluent reservoir is filled and the tubing connecting the reservoir to the ICS-2100 tubing is securely connected (see Figure 3-8). 2. Make sure the eluent generator electrical cable (blue) is connected to the connector.
Page 70: Set System Operating Conditions
ICS-2100 Ion Chromatography System reminder message appears. The pump will begin pumping at approximately 3 mL/min. 4. Continue priming the ICS-2100 until all air and previous eluent are purged and no air bubbles are exiting the waste line. 5. Press Pump Off 6.
Page 71: Equilibrate The System And Verify Operational Status
3 • Operation and Maintenance 5. Verify that the column heater is set to the correct value. Automatically Turning on the Suppressor and Eluent Generator You can set up the ICS-2100 to turn on the suppressor, eluent generator, and CR-TC automatically when the pump is turned on. To do this: •...
Page 72: Prepare Samples
ICS-2100 Ion Chromatography System • If the pressure is too high, there may be a restriction in the system plumbing. See Section 4.7 for troubleshooting information. 5. Verify that the baseline conductivity is at the expected reading for your μ application and is stable.
Page 73: Pretreating Samples
3 • Operation and Maintenance Analyze samples containing nitrite or sulfite as soon as possible. Nitrite oxidizes to nitrate, and sulfite to sulfate, thus increasing the measured concentrations of these ions in the sample. In general, samples that do not contain nitrite or sulfite can be refrigerated for at least one week with no significant changes in anion concentrations.
Page 74: Loading And Injecting Samples
ICS-2100 Ion Chromatography System 3.10 Loading and Injecting Samples NOTE Samples can be injected using either the standard injection valve or the optional auxiliary valve, if installed. For more information, see the Chromeleon Help or user’s manual. The two techniques for loading samples into the sample loop are: (1) with an autosampler or (2) with a syringe or vacuum syringe through the injection port on the ICS-2100 front door.
Page 75: Loading Samples With A Syringe
3 • Operation and Maintenance Setup for an AS-HV Autosampler For setup information for the AS-HV Autosampler, see the AS-HV Autosampler Operator’s Manual (Document No. 065125). The manual is provided on the Dionex Reference Library CD-ROM (P/N 053891). 3.10.1 Loading Samples with a Syringe 1.
Page 76: Loading Samples With A Vacuum Syringe
ICS-2100 Ion Chromatography System 3.10.2 Loading Samples with a Vacuum Syringe 1. Disconnect the waste line from port of the injection valve (see W (6) Figure 3-10) and attach a shorter line: 25 to 30 cm (10 to 12 inches) of ®...
Page 77: Injecting Samples
3 • Operation and Maintenance 3.10.4 Injecting Samples After loading the sample in the sample loop, use one of the following methods to switch the injection valve to the Inject position: • Manually: Click the button on the Chromeleon Control panel Inject (see Figure...
Page 78: Automatic (Batch) Sample Processing
ICS-2100 Ion Chromatography System 4. Click the button on the Control panel or the touch screen Autozero page. HOME 5. Inject the sample (see Section 3.10.4). 6. In Chromeleon, monitor the chromatogram on the Control panel and select Control > Acquisition Off when sample data is collected. NOTE Data from Chromeleon manual processing is saved in the MANUAL sequence folder under the local datasource.
Page 79 3 • Operation and Maintenance Summary of Automatic Sample Processing 1. Complete the instructions in Section 3.2 through Section 3.8 prepare the ICS-2100 for operation and to prepare the sample for processing. 2. If an autosampler is installed, prepare and fill the sample vials and place them in the autosampler tray or cassette.
Page 80: Maintenance
ICS-2100 Ion Chromatography System c. Click Next > and select the in a new sequence via Sequence Wizard option. d. Click Next > to go to the Sequence Wizard. e. Complete the steps in the Sequence Wizard, adding the desired number of samples and standards to the list.
Page 81 3 • Operation and Maintenance friction and wear may gradually result in small liquid leaks around the piston seal. If unchecked, these leaks can gradually contaminate the piston housing, causing the pump to operate poorly. If leaks occur, replace the piston seals (see Section 5.7).
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Page 83: Troubleshooting
4 • Troubleshooting This chapter is a guide to troubleshooting minor issues that may arise during operation of the ICS-2100. Turn to the section of this chapter that best describes the operating problem or symptom that has been observed. Each section lists possible causes of the problem or symptom in order of probability.
Page 84 ICS-2100 Ion Chromatography System The table below lists the most frequently observed ICS-2100 error messages. For troubleshooting assistance, refer to the page indicated in the table. Alarms and Error Conditions Auxiliary power supply disconnected page 74 Auxiliary power supply over current page 74 Auxiliary power supply over voltage page 75...
Page 85 4 • Troubleshooting Alarms and Error Conditions Option not installed page 81 Pressure slope calibration error page 81 Pump motor lost control page 81 Pump over pressure page 82 Pump pressure hardware error page 82 Pump stopped due to lost USB communication error page 82 Pump under pressure page 83...
Page 86: Troubleshooting Error Messages
ICS-2100 Ion Chromatography System Troubleshooting Error Messages AUXILIARY POWER SUPPLY DISCONNECTED This error occurs when the auxiliary power supply is configured as active, but no device is connected. To troubleshoot: 1. Connect the cable for the auxiliary device to the power supply EGC-2 connector.
Page 87: Auxiliary Power Supply Over Voltage
4 • Troubleshooting AUXILIARY POWER SUPPLY OVER VOLTAGE This error occurs when the device connected to the auxiliary power supply is attempting to operate at a higher voltage than is allowed (35 V). To troubleshoot: 1. Check the cable connecting the device to the power supply for damage or a faulty connection.
Page 88: Column Heater Open Circuit
ICS-2100 Ion Chromatography System COLUMN HEATER OPEN CIRCUIT This error occurs when the column heater is unplugged from the component panel. To troubleshoot: 1. Check that the column heater is plugged into the component panel. 2. If the error persists, the column heater may be faulty. Replace the column heater (see Section 5.12).
Page 89: Cr-Tc Stopped For Zero Flow Rate
4 • Troubleshooting CR-TC STOPPED FOR ZERO FLOW RATE This message appears when you click the Shutdown button on the Chromeleon Control panel. The message also appears when you turn off the pump flow while the EGC current (and CR-TC) are on. The CR-TC is automatically turned off.
Page 90: Calibration Error
ICS-2100 Ion Chromatography System EGC-1 CALIBRATION ERROR EGC-2 CALIBRATION ERROR This error occurs if the measured current is outside the expected range when running the eluent generator calibration procedure. To troubleshoot: 1. Review the procedure to verify that you set up the ICS-2100 correctly and then rerun the calibration.
Page 91: Invalid Flow Rate
4 • Troubleshooting your application. Reset the eluent concentration and/or the flow rate to the recommended values. EGC-1 INVALID FLOW RATE ERROR EGC-2 INVALID FLOW RATE ERROR This error occurs if the flow rate is set to a value not supported by the eluent generator.
Page 92: Hardware Not Present
ICS-2100 Ion Chromatography System HARDWARE NOT PRESENT This error indicates a problem in the ICS-2100 electronics. To troubleshoot: Contact Dionex for assistance. The ICS-2100 electronics components cannot be serviced by the user. LEAK SENSOR WET The leak sensor is installed in the drip tray at the bottom of the component panel (see Figure 2-8).
Page 93: Option Not Installed
4 • Troubleshooting 3. Turn off the ICS-2100 power briefly and then restart. 4. If the problem persists, repeat Step 5. If the problem persists, contact Dionex for assistance. OPTION NOT INSTALLED This error occurs if a command is issued to control an option that is not installed.
Page 94: Pump Over Pressure
ICS-2100 Ion Chromatography System PUMP PRESSURE HARDWARE ERROR This error indicates a problem in the pump controller electronics. To troubleshoot: Contact Dionex for assistance. The ICS-2100 electronics components cannot be serviced by the user. PUMP OVER PRESSURE If the system pressure exceeds the set limit for at least 0.5 second, this error message appears and the pump stops.
Page 95: Pump Under Pressure
4 • Troubleshooting PUMP UNDER PRESSURE If the system pressure falls below the low pressure limit, the pump stops and this error message appears. Pressure limits can be set in the Chromeleon Server Configuration or in the control program. The limits can also be set on the touch screen page (see Section...
Page 96: Suppressor Not Connected
ICS-2100 Ion Chromatography System SUPPRESSOR NOT CONNECTED This error occurs if you turn on the suppressor and the ICS-2100 cannot establish a connection with the suppressor. To troubleshoot: 1. Check the suppressor cable connection (see Section 5.11). 2. If the error persists, contact Dionex for assistance. There may be a problem in the suppressor controller electronics.
Page 97: Suppressor Over Power
4 • Troubleshooting SUPPRESSOR OVER POWER This error occurs when, in order to maintain the selected current, the ICS- 2100 is required to apply a higher voltage than the suppressor can support. To troubleshoot: 1. Reduce the flow rate. 2. Rehydrate the suppressor. Refer to the suppressor manual for instructions. Suppressor manuals are included on the Dionex Reference Library CD- ROM (P/N 053891).
Page 98: Liquid Leaks
ICS-2100 Ion Chromatography System Liquid Leaks • Leaking fitting Locate the source of the leak. Tighten or, if necessary, replace the liquid line connection (see Section 5.3). Refer to Installation of Dionex Liquid Line Fittings (Document No. 031432) for tightening requirements. The manual is included on the Dionex Reference Library CD-ROM (P/N 053891).
Page 99 4 • Troubleshooting • Leaking pressure transducer 1. Make sure the liquid line connections into the pressure transducer are tight. Refer to Installation of Dionex Liquid Line Fittings (Document No. 031432) for tightening requirements. The manual is included on the Dionex Reference Library CD-ROM (P/N 053891).
Page 100: Pump Difficult To Prime Or Loses Prime
ICS-2100 Ion Chromatography System Pump Difficult to Prime or Loses Prime Excessive pressure fluctuations (more than 3% difference from one pressure reading to the next) indicate that the pump is out of prime. • Empty eluent reservoir and/or no eluent connected Fill the reservoir.
Page 101 4 • Troubleshooting 4. Check the Degas settings. Select either the Monitor option or the Cycle option. If you select Cycle, set the time On to 30 seconds and the time Off to 600 seconds. If Cycle is already selected, try increasing the time on or decreasing the time off.
Page 102: Pump Does Not Start
ICS-2100 Ion Chromatography System Pump Does Not Start • No power (front LED indicator is not lit) Power Check that the power cord is plugged in. Check the main power fuses and replace, if needed (see Section 5.18). • No communication between ICS-2100 and Chromeleon Verify that the USB cable is connected correctly.
Page 103: Erratic Flow/Pressure Reading
4 • Troubleshooting Waste Valve Priming Valve Figure 4-2. Waste and Priming Valves • Flow rate is set to 0 Set the flow rate from the ICS-2100 Control panel in Chromeleon or from the touch screen page. HOME • Eluent valve is closed Open the eluent valve from the ICS-2100 Control panel in Chromeleon or from the touch screen page.
Page 104: Excessive System Backpressure
ICS-2100 Ion Chromatography System • Damaged piston seal Replace the piston seal (see Section 5.7). • Dirty pump check valve Clean or replace the check valve (see Section 5.6). • Leaking liquid lines or fittings Check the liquid lines and fittings for small leaks. Tighten or, if necessary, replace the liquid line connection (see Section 5.3).
Page 105: Peak "Ghosting
4 • Troubleshooting • Plugged injection valve or auxiliary valve passages Rebuild the valve (see Section 5.4). Peak “Ghosting” “Ghosting” is the appearance of extraneous peaks in a chromatogram. These may be late-eluting peaks from a previous injection. They may also be the result of a contaminated standard or eluent, or a malfunctioning injection valve.
Page 106: Nonreproducible Peak Height Or Retention Time
ICS-2100 Ion Chromatography System 4.10 Nonreproducible Peak Height or Retention Time • Column overloading Dilute the sample (see Section 3.9.3). • Liquid leaks Locate and eliminate the leaks (see Section 4.3). • Incomplete or imprecise filling of the sample loop 1.
Page 107: High Cell Output
4 • Troubleshooting • No flow from pump This condition has several possible causes; see Section 4.5 Section 4.6 details. • Cell electronics malfunctioning Use the Chromeleon Wellness Panel diagnostics to test the electronics (see Section 5.1 and the Chromeleon Help for instructions). If the variance reading is outside the tolerance range (less than 1 μS), the electronics are malfunctioning.
Page 108: Baseline Noise Or Drift
ICS-2100 Ion Chromatography System 4.14 Baseline Noise or Drift • Flow system leak; erratic baseline Check all fittings and liquid lines for leaks. Tighten or, if necessary, replace all liquid line connections. Refer to Installation of Dionex Liquid Line Fittings (Document No.
Page 109: Vacuum Degas Assembly Does Not Run
4 • Troubleshooting • Cell above or below temperature Contact Dionex for assistance. • Damaged piston seal Replace the piston seal (see Section 5.7). 4.15 Vacuum Degas Assembly Does Not Run • Degas option not enabled in Chromeleon 1. Open the Chromeleon Server Configuration program. Right-click the ICS-2100 device in the timebase and select Properties.
Page 110 ICS-2100 Ion Chromatography System 4. Under Degas, verify that the Always Off option is not selected. If it is, select one of the following settings instead: • Cycle; also select On: 30 seconds and Off: 10 minutes • Monitor 5. If the Degas settings are correct, but the degas assembly still does not run, test the assembly by selecting the Always On option.
Page 111: Service
5 • Service This chapter describes ICS-2100 service and repair procedures that the user can perform. All procedures not included here, including electronics-related repair procedures, must be performed by Dionex personnel. For assistance, contact Dionex Technical Support. In the U.S., call 1-800-346-6390. Outside the U.S., call the nearest Dionex office.
Page 112: Chromeleon Wellness Panel Overview
ICS-2100 Ion Chromatography System 5.1.1 Chromeleon Wellness Panel Overview NOTE Do not open a System Wellness Control panel if the name includes “Service.pan.” These Wellness panels are reserved for use by Dionex Service Representatives. To Open the Wellness Panel 1. In the Chromeleon Browser, expand the Dionex Templates\Panels\Wellness folder.
Page 113 5 • Service Use the Wellness Panel to perform the following functions: • Calibrate the pump flow rate • Calibrate the vacuum degas assembly • Calibrate the conductivity cell • Test the conductivity cell electronics, using a dummy cell • Upload calibration values from the ICS-2100 to the Chromeleon Wellness database After completing a calibration procedure from the touch screen,...
Page 114: Diagnostic And Calibration Touch Screen Overview
ICS-2100 Ion Chromatography System 5.1.2 Diagnostic and Calibration Touch Screen Overview Select from the touch screen menu of pages. The DIAGNOSTIC page opens (see Figure 5-2). From there, you can access DIAGNOSTIC individual diagnostic and calibration pages. NOTE The Service button provides access to service functions that are performed only by Dionex personnel.
Page 115: Calibrating The Conductivity Cell
5 • Service 5.1.3 Calibrating the Conductivity Cell When to Calibrate • After every 6 months of use NOTE Do not use this procedure to calibrate a new cell. If you are replacing a cell, see the instructions in Section 5.10 to install the new cell and calibrate it.
Page 116 ICS-2100 Ion Chromatography System 2. On the Wellness Panel under Electric Conductivity Cell Calibration, click Offset Cal, or touch Run Offset Cal on the ICS- 2100 touch screen. 3. When the offset calibration is complete, click Slope Cal on the Wellness Panel, or touch on the ICS-2100 touch Run Slope Cal...
Page 117 5 • Service let it stabilize. When the temperature has stabilized, “=” is displayed to the right of the temperature reading on the CONDUCTIVITY CELL page. 11. Select 1.00 mL/min to begin pumping 1.00 mM KCl through the cell at 1.0 mL/min. 12.
Page 118: Calibrating The Flow Rate
ICS-2100 Ion Chromatography System 5.1.4 Calibrating the Flow Rate When to Calibrate If you run the ICS-2100 Operational Qualification or Performance Qualification and it fails. Items Needed • 0.076-mm (0.003-in) ID yellow PEEK tubing (P/N 049715) to create 14 ± 2 MPa (2000 ± 300 psi) of backpressure (if needed) •...
Page 119 5 • Service 3. On the Chromeleon Wellness Panel under Pump Flow Rate Calibration, select Reset Cal, or touch on the touch Reset Cal screen. 4. Select 1 mL/min to set the flow rate and begin pumping deionized water. 5. Allow the pump to stabilize for 20 minutes. 6.
Page 120: Calibrating The Vacuum Degas Assembly
ICS-2100 Ion Chromatography System 5.1.5 Calibrating the Vacuum Degas Assembly When to Calibrate • After every 6 months of use 1. Open the ICS-2100 Wellness Panel in Chromeleon (see Section 5.1.1), or open the ICS-2100 touch screen page PUMP DEGAS (see Figure 5-6).
Page 121: Isolating A Restriction In The Liquid Lines
5 • Service Isolating a Restriction in the Liquid Lines A restriction in the liquid plumbing will cause excessive system backpressure. 1. Begin pumping eluent through the system (including the columns). 2. Follow the flow schematics in Figure 5-7, Figure 5-8, or Figure 5-9 and work...
Page 122 ICS-2100 Ion Chromatography System To the pump Degas In EGC Vent EGC II KOH, LiOH, MSA, or NaOH Deionized NOTE: The EGC Water Degas fittings cannot be serviced by the user. Inlet Outlet Degas CR-TC From the pulse damper Degas In To the Column inject valve...
Page 123 5 • Service To the pump Degas In EGC Vent NOTE: The EGC II EGC degas fittings Deionized cannot be Water serviced by the user Inlet Outlet Eluent Out Regen Out Eluent In Degas Regen In Degas In Column Heater Degas Out Conductivity Cell...
Page 124 ICS-2100 Ion Chromatography System To the pump Degas In EGC Vent NOTE: The EGC II EGC degas fittings cannot Deionized be serviced by Water the user Inlet Outlet Degas Degas In Column Heater Degas Out Conductivity Cell Suppressor Cell In Cell Out Pulse Damper Valve...
Page 125: Replacing Tubing And Fittings
5 • Service Replacing Tubing and Fittings The ICS-2100 is plumbed with the tubing and tubing assemblies listed below. Tubing Size and Type Color Used For 0.125-mm (0.005-in) ID PEEK Connection from pump pulse damper (P/N 044221) to pressure transducer 0.25-mm (0.010-in) ID PEEK Black Connections between other system...
Page 126: Rebuilding The Injection Valve Or Auxiliary Valve
ICS-2100 Ion Chromatography System Rebuilding the Injection Valve or Auxiliary Valve Dionex recommends rebuilding the injection valve and the auxiliary valve (if installed) annually. The Injection Valve Rebuild Kit (P/N 057896) contains all required replacement parts for one valve. NOTE If you prefer, you can replace the auxiliary valve “pod,” instead of rebuilding the valve.
Page 127 5 • Service Figure 5-10. Injection Valve Plumbing NOTE A backpressure coil may be installed between port P and the line labeled TO INJECT VALVE - P Doc. 065291-01 3/09...
Page 128: Replacing An Auxiliary Valve Pod
ICS-2100 Ion Chromatography System Replacing an Auxiliary Valve Pod This procedure describes how to replace the mechanical part (the “pod”) of the optional auxiliary valve. This procedure is an alternative to rebuilding the valve (see Section 5.4). NOTE If the valve electronics require service, contact Dionex. Electronics-related repair procedures...
Page 129 5 • Service 8. Also verify that the two splines on the pod align with the matching splines inside the valve holder (see Figure 5-12). If necessary, twist the end of the pod to adjust the position of the splines. Valve Pod Valve Holder Slot (larger)
Page 130: Cleaning And Replacing The Pump Check Valves
ICS-2100 Ion Chromatography System Cleaning and Replacing the Pump Check Valves A dirty check valve causes erratic flow rates and pressures. In addition, it may cause the pump to lose prime and/or be difficult to reprime. If possible, replace dirty check valves. If new check valves are not available, follow the instructions for cleaning.
Page 131 5 • Service 6. If you are installing new cartridges (P/N 045994) in the existing check valve Single-hole Double-hole housings, place the cartridge in the inlet check valve housing with the double-hole end of the cartridge visible and place the cartridge in the outlet housing with the single-hole end visible.
Page 132: Replacing A Pump Piston Seal And Piston Rinse Seal
ICS-2100 Ion Chromatography System Replacing a Pump Piston Seal and Piston Rinse Seal A damaged seal allows leakage past the piston, as well as leakage from the piston seal wash housing. The pump may be difficult to prime, flow rates may be unstable, and there may be baseline noise.
Page 133 5 • Service 5. Place the head (front end down) on a clean work surface and lift off the spacer to expose the piston seal (see Figure 5-14 Figure 5-15). Priming Valve Knob (P/N 055709) Outlet Check Valve Assembly (P/N 045721) O-Ring (P/N 055752) Piston Seal...
Page 134 ICS-2100 Ion Chromatography System 6. The piston does not come off as part of the pump head assembly because it is captured by a magnetic retention system. After removing the pump head, hold the shaft of the piston (near the base), tilt the piston slightly, and pull the piston away from the pump.
Page 135 5 • Service Removing the Piston Seal from the Head 1. Fill the head cavity with deionized water by injecting the liquid through either the piston opening or the inlet check valve. 2. Reinsert the piston approximately 3 mm (0.125) inch into the seal. 3.
Page 136: Replacing A Pump Piston
ICS-2100 Ion Chromatography System Reinstalling the Head and Piston Dionex recommends reinstalling the head and piston as a single assembly, so that the piston centers itself onto the magnetic follower. 1. Hold the assembled spacer and guide with the drain tubes aligned vertically and press the spacer into the head until it is flush with the indented surface of the head.
Page 137: Replacing The Waste Valve Or Priming Valve O-Ring
5 • Service Replacing the Waste Valve or Priming Valve O-Ring A damaged O-ring causes leakage around the base of the waste valve or priming valve knob. 1. Close the eluent valve from the ICS-2100 Control panel in Chromeleon or select on the touch screen page.
Page 138 ICS-2100 Ion Chromatography System 4. If the O-ring is removed with the valve knob in Step 3, pull the O-ring off the end of the knob (see Figure 5-17). If the O-ring is not removed with the valve knob, insert a thin object (for example, the bent end of a paper clip) into the cavity in the pump head and carefully pull out the O-ring.
Page 139: Replacing The Conductivity Cell
5 • Service 5.10 Replacing the Conductivity Cell 1. Turn off the ICS-2100 power. 2. Open the front door and disconnect the tubing from the CELL IN CELL fittings (see Figure 5-18, View A). Loosen the two screws on the cell front cover.
Page 140 ICS-2100 Ion Chromatography System 5. Slide the new cell assembly straight into the opening in the component panel until it stops and the cell cover is flush against the component panel. The cell’s electronic connector automatically plugs into a connector inside the ICS-2100 when you push the assembly into the opening.
Page 141 5 • Service To calibrate the cell on the ICS-2100 touch screen page: DIAGNOSTIC a. Go to the page and touch Electric Conductivity Cell DIAGNOSTIC b. On the page (see Figure 5-20), ELECTRIC CONDUCTIVITY CALIBRATION touch Run Offset Cal Figure 5-20. Electric Conductivity Calibration Page c.
Page 142: Replacing The Suppressor
ICS-2100 Ion Chromatography System 5.11 Replacing the Suppressor Refer to the suppressor manual for guidance about when to replace a suppressor. Suppressor manuals are included on the Dionex Reference Library CD-ROM (P/N 053891). 1. Refer to the suppressor quick start guide (shipped with the suppressor) for instructions on preparing the suppressor before initial use and for additional installation details.
Page 143: Replacing The Column Heater
5 • Service 10. Push the cables into the opening in the component panel. 11. Align the top of the suppressor with the guide line printed on the component panel (see Figure 5-22) and align the slots on the rear of the suppressor Suppressor with the tabs on the panel.
Page 144 ICS-2100 Ion Chromatography System Thumbscrews (6) Figure 5-23. ICS-2100 Column Heater Cover Removed d. Remove the columns from the column heater. e. Pull the column heater straight toward you to remove it from the component panel. 4. Before installing the new column heater, write down the two calibration values (offset and slope) recorded on the back of the heater.
Page 145 5 • Service 9. Enter the column heater calibration values in Chromeleon or on the ICS-2100 touch screen page as follows. DIAGNOSTIC To enter the calibration values in Chromeleon: a. Open the Wellness Panel (see Section 5.1.1) and click Calibration Details.
Page 146: Replacing The Column Heater Heat Exchanger
ICS-2100 Ion Chromatography System c. To ensure the calibration values are recorded in the ICS-2100 memory, do not turn off the ICS-2100 power for at least 1 minute after entering the values. d. Record the calibration values in the Chromeleon Wellness database. To do this, open the Wellness Panel (see Section 5.1.1) and click Upload under...
Page 147: Replacing The Eluent Valve
5 • Service tubing) and press the heat exchanger onto the heater. Replace the screws removed in Step 10. Connect the new heat exchanger inlet line to port C on the injection valve and connect the outlet line to the guard column inlet. 11.
Page 148 ICS-2100 Ion Chromatography System 6. Pull the valve straight out from the component panel and begin pulling the attached cable out of the opening in the panel. Disconnect cables 7. The cable is connected to a matching cable inside the ICS-2100. Continue pulling until the Eluent Valve connectors for the two cables are...
Page 149: Replacing The Leak Sensor
5 • Service 12. Align the new valve on the component panel with the liquid ports facing up and attach it with the two screws removed in Step 5. Reconnect the liquid lines. 13. Turn on the power to the ICS-2100. 14.
Page 150: Priming The Pump
ICS-2100 Ion Chromatography System 7. Connect the cable from the new leak sensor (P/N 058053) to the ICS-2100 cable. 8. Feed the cables back inside the ICS-2100. Align the leak sensor with the component panel opening and fingertighten the screw. 9.
Page 151 5 • Service Secondary Pump Head Waste Valve (close) Port Primary Pump Head Priming Valve 10 mL Syringe (open) (P/N 054578) Figure 5-31. Priming the Eluent Lines 3. Open the priming valve by turning it one-quarter to one-half turn counterclockwise. 4.
Page 152: Priming With The Prime Button
ICS-2100 Ion Chromatography System 5.16.2 Priming with the Prime Button NOTE Perform this procedure after priming the eluent lines (see Section 5.16.1). 1. Check that the priming valve on the primary pump head is closed (see Figure 5-32). Secondary Pump Head Waste Valve (open) Primary...
Page 153: Priming The Pump With Isopropyl Alcohol
5 • Service 5.17 Priming the Pump with Isopropyl Alcohol NOTE Prime the pump heads with isopropyl alcohol only if the standard priming procedures described Section 5.16 are unsuccessful. 1. Connect a 10-mL syringe (P/N 054578) filled with Secondary Pump Head isopropyl alcohol (IPA) to the port in the primary pump head (see...
Page 154: Changing Main Power Fuses
ICS-2100 Ion Chromatography System Isopropyl alcohol may damage some columns. Be sure to thoroughly rinse the alcohol from the pump, using the process described in Step 8. Close the waste valve. Do not overtighten. 9. Select the flow rate required for the analysis. 10.
Page 155: Replacing An Elugen Cartridge
5 • Service 3. Replace the two fuses in the holder with new IEC 127 fast-blow fuses rated 3.15 amps (P/N 954745). Dionex recommends always replacing both fuses. 4. Reinsert the fuse holder into its compartment and push in until the tab clicks in place.
Page 156 ICS-2100 Ion Chromatography System 4. Remove the EluGen Luer Adapter cartridge line VENT Luer Lock (see Figure 5-35) from the EluGen cartridge by unscrewing the luer Vent lock from the luer Line adapter. If necessary, use a wrench to hold the luer adapter in place while unscrewing the vent line.
Page 157 5 • Service Figure 5-37. EluGen Cartridge in the Service Area 8. Disconnect the EGC IN lines EGC OUT EGC OUT OUTLET from the EGC IN INLET Port ports on the OUTLET INLET EluGen cartridge (see Port Figure 5-38). Figure 5-38. Disconnect the EluGen Cartridge Inlet and Outlet Port Lines 9.
Page 158 ICS-2100 Ion Chromatography System 10. Remove the new EluGen cartridge from the shipping box. 11. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the EluGen cartridge INLET OUTLET ports (see OUTLET Figure 5-39). INLET Figure 5-39.
Page 159 5 • Service 14. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) in the ICS-2100 Ship Kit Backpressure Coil (P/N 064375). 15. Connect one end of OUTLET this coil to the Port EluGen cartridge port (see OUTLET Figure 5-41).
Page 160 ICS-2100 Ion Chromatography System 17. Slide the EluGen cartridge down into the holder until it stops. Make sure the cable and backpressure coil remain out of the holder (see Figure 5-42). NOTE To avoid crimping the lines inside the holder, do not twist the cartridge before you slide it into the holder.
Page 161 5 • Service 19. Remove the plug from the EluGen cartridge vent opening and connect the EluGen cartridge line. VENT Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on the EluGen cartridge label. 1. Open the Chromeleon Server Configuration program (select Start > All Programs >...
Page 162 ICS-2100 Ion Chromatography System Conditioning the EluGen Cartridge Always condition the EluGen cartridge before initial use. This requires directing the backpressure coil connected to the EluGen cartridge outlet to waste, and then generating 50 mM of eluent at 1.00 mL/min for 30 minutes.
Page 163: Replacing A K2Co3 Cartridge
5 • Service 11. Lift the EluGen cartridge out of the EGC OUT holder, turn it over, EGC IN OUTLET and set it in the Port INLET service area. Port 12. Disconnect the backpressure coil from the EluGen cartridge OUTLET port 13.
Page 164 ICS-2100 Ion Chromatography System 4. Remove the EluGen Luer Adapter cartridge line VENT Luer Lock (see Figure 5-46) from the EluGen cartridge by unscrewing the luer Vent lock from the luer Line adapter. If necessary, use a wrench to hold the luer adapter in place while unscrewing the vent line.
Page 165 5 • Service Figure 5-48. EluGen Cartridge In the Service Area 8. Disconnect the EGC IN lines EGC OUT EGC OUT OUTLET from the EGC IN INLET Port fittings on the OUTLET INLET EluGen cartridge (see Port Figure 5-49). Figure 5-49. Disconnect the EluGen Cartridge Inlet and Outlet Port Lines 9.
Page 166 ICS-2100 Ion Chromatography System 10. Remove the new EluGen cartridge from the shipping box. 11. Orient the EluGen cartridge with the fittings facing up and remove the plugs from the EluGen cartridge INLET OUTLET ports (see OUTLET Figure 5-50). INLET Figure 5-50.
Page 167 5 • Service 15. Turn the EluGen cartridge over (with the fittings facing down). Shake the EluGen cartridge vigorously, and tap it with the palm of your hand 10 to 15 times. Check that all bubbles trapped in the eluent generation chamber are dislodged.
Page 168 ICS-2100 Ion Chromatography System 17. Remove the plug from connector on EGC-1 top of the ICS-2100. 18. Orient the cartridge’s blue 4-pin electrical cable connector as shown in Figure 5-53. Push it onto the EGC-1 connector on the ICS- 2100. Twist the ring on the cable connector fingertight to secure it.
Page 169 5 • Service Recording the EluGen Cartridge Serial Number in Chromeleon The serial number is printed on a label on the EluGen cartridge. 1. Open the Server Configuration program (select Start > All Programs > Chromeleon > Server Configuration). 2. Double-click the ICS-2100 system icon. The properties dialog box for the ICS-2100 appears.
Page 170 ICS-2100 Ion Chromatography System Continue on to one of the following sections, depending on the type of eluent to be generated: • For carbonate eluent, go to “Plumbing the EluGen Cartridge for Conditioning with Carbonate Eluent” on page 159. • For carbonate/bicarbonate eluent, go to “Plumbing the EluGen Cartridge and EPM for Conditioning with Carbonate/Bicarbonate...
Page 171 5 • Service Plumbing the EluGen Cartridge for Conditioning with Carbonate Eluent Figure 5-55 illustrates the liquid flow path during the EluGen cartridge conditioning procedure with carbonate eluent. EGC VENT EGC II K CO Send to waste Outlet Inlet TO DEGAS ELUENT IN DEGAS TO DEGAS...
Page 172 ICS-2100 Ion Chromatography System 1. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet. 2. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) in the ICS- 2100 Ship Kit (P/N 064375).
Page 173 5 • Service Conditioning the EluGen Cartridge Always condition the EluGen cartridge for 30 minutes before initial use. 1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water. 2. Verify that the suppressor and the auxiliary electrolytic device attached to the connector (if installed) are off.
Page 174 ICS-2100 Ion Chromatography System Plumbing the EluGen Cartridge and EPM for Conditioning with Carbonate/Bicarbonate Eluent Figure 5-57 illustrates the liquid flow path through the eluent generator during conditioning with carbonate/bicarbonate eluent. EGC VENT EGC II K CO Send to waste Inlet Outlet (red...
Page 175 5 • Service 1. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and 10-32 to 1/4-28 coupler (P/N 042806) in the ICS- 2100 Ship Kit (P/N 064375). 2. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet.
Page 176 ICS-2100 Ion Chromatography System Conditioning the EluGen Cartridge Always condition the EluGen cartridge for 30 minutes before initial use. 1. Make sure the eluent reservoir is filled with ASTM filtered, Type I (18-megohm) deionized water. 2. Verify that the suppressor and the auxiliary electrolytic device (if installed) are off.
Page 177: Replacing The Cr-Tc
5 • Service 5.20 Replacing the CR-TC 1. Turn off the pump from the ICS-2100 Control panel in Chromeleon or from the touch screen page. HOME 2. Follow the instructions in Section 5.19.1, Step 2 through Step 8 to disconnect the EluGen cartridge and CR-TC cables, cap the EluGen cartridge vent opening, remove the EluGen cartridge from the holder, and disconnect the inlet and outlet tubing.
Page 178 ICS-2100 Ion Chromatography System 10. Check that none of the lines are caught under the CR-TC and then push the CR-TC onto the metal stud inside the EGC holder. Push down until the CR-TC snaps into place (see Figure 5-60). Figure 5-60.
Page 179 5 • Service 15. Reinstall the EluGen cartridge in the holder. 16. Remove the cap on the luer adapter by twisting. Save the cap. 17. Twist the luer lock and line into the luer adapter. EGC VENT 18. Do not connect the EluGen cartridge cable until after hydrating the CR-TC.
Page 180 ICS-2100 Ion Chromatography System 1. Locate the 10-32 to 1/4-28 coupler (P/N 042806) in the ICS-2100 Ship Kit (P/N 064375). 2. Use the coupler to connect the line labeled TO INJ VALVE IN - and the line labeled P/ELUENT OUT SRS/AES REGEN OUT 3.
Page 181: Replacing The Epm Electrolytic Ph Modifier
5 • Service 5.21 Replacing the EPM Electrolytic pH Modifier 1. Turn off the pump from the ICS-2100 Control panel in Chromeleon or from the touch screen page. HOME 2. Disconnect the EPM electrical cable from the connector on the ICS- EGC-2 2100 top cover.
Page 182: Recording The Epm Serial Number In Chromeleon
ICS-2100 Ion Chromatography System 5.21.1 Recording the EPM Serial Number in Chromeleon The serial number is printed on a label on the EPM. 1. Open the Server Configuration program (select Start > All Programs > Chromeleon > Server Configuration). 2. Double-click the ICS-2100 system icon. The properties dialog box for the ICS-2100 appears.
Page 183: Plumbing The Epm For Hydrating And Conditioning
5 • Service 5.21.2 Plumbing the EPM for Hydrating and Conditioning Figure 5-57 illustrates the liquid flow path during hydration and conditioning. EGC VENT EGC II K CO Send to waste Inlet Outlet (red label) TO CR-TC/EPM (yellow label) ELUENT OUT (yellow label) TO DEGAS ELUENT IN...
Page 184 ICS-2100 Ion Chromatography System 1. Disconnect the line from the EGC TO INJ VALVE IN - P/ELUENT OUT Mixer inlet. 2. Use a 10-32 to 10-32 coupler (P/N 042627) to connect the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) to the line.
Page 185: Hydrating And Conditioning The Epm
5 • Service 5.21.3 Hydrating and Conditioning the EPM Follow the steps below to hydrate the EPM for 5 minutes and then condition the EPM for 30 minutes. 1. Fill the eluent reservoir with ASTM filtered, Type I (18-megohm) deionized water. 2.
Page 186: Replacing The Egc Co3 Mixer
ICS-2100 Ion Chromatography System 14. Reconnect the line to the EGC CO TO INJ VALVE IN - P/ELUENT OUT Mixer inlet. 15. Reconnect the line to the suppressor TO SRS/AES REGEN OUT REGEN port. 5.22 Replacing the EGC CO Mixer 5.22.1 Installing the New EGC CO Mixer 1.
Page 187: Filling The Egc Co3 Mixer With Deionized Water
5 • Service 4. Connect the line to the mixer inlet and connect TO EGC CO3 MIXER IN line to the mixer outlet. TO EGC CO3 MIXER OUT TO EGC CO MIXER OUT TO EGC CO MIXER IN Figure 5-67. ICS-2100 with an EGC CO Mixer Installed 5.22.2 Filling the EGC CO Mixer with Deionized Water...
Page 188 ICS-2100 Ion Chromatography System TO PUMP/ DAMPER (Disconnect this line) Pump Outlet (Green tubing) Figure 5-68. Disconnect the Cartridge Inlet and Pump Outlet 3. Disconnect the line from the TO EGC CO3 MIXER IN TO INJ VALVE IN - line. P/ELUENT OUT Doc.
Page 189 5 • Service 4. Connect the pump outlet to the mixer inlet (see Figure 5-69). Pump Outlet Mixer Inlet Figure 5-69. Pump Outlet Connected to Mixer Inlet for Mixer Hydration 5. Disconnect the mixer outlet line (labeled ) from TO INJ VALVE PORT P port ) on the injection valve.
Page 190: Filling The Egc Co3 Mixer With Eluent
ICS-2100 Ion Chromatography System 11. Run the pump until the mixer is filled and there is a consistent flow of water exiting the mixer outlet. It takes about 5 minutes to fill a 4-mm mixer and about 2 minutes to fill a 2-mm mixer.
Page 191 5 • Service Filling the EGC CO Mixer with Carbonate Eluent Figure 5-70 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO Mixer with carbonate eluent. To the EluGen From the EluGen cartridge inlet port cartridge outlet port TO DEGAS...
Page 192 ICS-2100 Ion Chromatography System 1. Locate the yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765) and a 10-32 to 10-32 coupler (P/N 042627) in the ICS- 2100 Ship Kit (P/N 064375). 2. Use the coupler to connect one end of the backpressure coil to the line.
Page 193 5 • Service 12. Run at these settings until a consistent flow of eluent is exiting the mixer outlet. At a flow rate of 1 mL/min it will take about 16 minutes to fill a 4-mm mixer and about 5 minutes to fill a 2-mm mixer. NOTE The void volume of the 4-mm mixer is about 16 mL.
Page 194 ICS-2100 Ion Chromatography System Filling the EGC CO Mixer with Carbonate/Bicarbonate Eluent Figure 5-71 illustrates the liquid flow path through the eluent generator components during initial filling of the EGC CO Mixer with carbonate/bicarbonate eluent. To the EluGen From the EluGen cartridge inlet port cartridge outlet port (red)
Page 195 5 • Service 1. Locate the following items in the ICS-2100 Ship Kit (P/N 064375): a yellow 0.5 mL/min, 7 MPa (1000 psi) backpressure coil (P/N 053765), a 10-32 to 10-32 coupler (P/N 042627), and a 10-32 to 1/4-28 coupler (P/N 042806). 2.
Page 196 ICS-2100 Ion Chromatography System 5. Verify that the suppressor and the auxiliary electrolytic device (if installed) are off. To avoid damaging the suppressor and auxiliary electrolytic device (if installed), always turn them off before filling the mixer. The pump flow is on during filling;...
Page 197: Replacing The Egc Holder And Degas Assembly
5 • Service 17. Reconnect the line to the suppressor TO SRS/AES REGEN OUT REGEN port. 18. Reconnect the mixer outlet line to port ) on the injection valve. 5.23 Replacing the EGC Holder and Degas Assembly 5.23.1 Disconnecting and Removing the EluGen Cartridge 1.
Page 198 ICS-2100 Ion Chromatography System 4. Remove the Luer Adapter line (see VENT Luer Lock Figure 5-73) from the EluGen cartridge by unscrewing the EGC Vent luer lock from the Line luer adapter. If necessary, use a wrench to hold the luer adapter in place while unscrewing the vent line.
Page 199 5 • Service 9. Disconnect the lines from the EGC IN EGC OUT INLET ports on the cartridge. OUTLET 10. Lift the EGC holder out of the top cover and pull the liquid lines through the tubing chase (see Figure 5-75) to remove them from the ICS- 2100.
Page 200: In The New Egc Holder
ICS-2100 Ion Chromatography System 5.23.2 Removing the CR-TC and Reinstalling it in the New EGC Holder 1. Turn the holder upside down to view the CR-TC. 2. Grasp the top of the CR- Disconnect liquid TC and lift up to lines from 4 ports disconnect it from the holder.
Page 201 5 • Service 5. Check that none of the lines are caught under the CR-TC and then push the CR-TC onto the metal stud inside the EGC holder. Push down until the CR-TC snaps in place (see Figure 5-78). Figure 5-78. CR-TC Installed in Holder 6.
Page 202: In The New Egc Holder
ICS-2100 Ion Chromatography System 9. Feed the following liquid lines from the bottom of the holder through the tubing chase to the ICS-2100 front component panel. • TO SRS/AES REGEN OUT • TO INJ VALVE IN - P/ELUENT OUT • TO PUMP/DAMPER 10.
Page 203 5 • Service 4. Thread the three lines from the EPM (red, yellow, and blue labels) through the opening on the side of the new holder (see Figure 5-80). TO CR-TC/EPM TO CR-TC/EPM ELUENT IN ELUENT OUT (red label) (yellow label) TO CR-TC/EPM REGEN OUT (blue label)
Page 204: Installing The New Egc Holder Without A Cr-Tc
ICS-2100 Ion Chromatography System 8. Turn the EGC holder right side up and set it in place on top of the ICS-2100. 9. Slide the EPM mounting bracket and connected EPM onto the side of the EGC holder. 10. Feed the following liquid lines through the tubing chase to the ICS- 2100 front component panel.
Page 205: Reinstalling The Elugen Cartridge
5 • Service • Connect the line labeled to the TO INJ VALVE IN - P/ELUENT OUT EGC CO Mixer inlet. • Connect the line labeled to the pulse damper. TO PUMP/DAMPER Direct the line from the top of the ICS- WASTE, GAS SEPARATOR 2100 to the rear panel.
Page 206 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 207: A • Specifications
A • Specifications A.1 Electrical Main Power 100 to 240 Vac, 50 to 60 Hz (Auto-sensing power supply; no manual voltage or frequency adjustment required) Fuses Two 3.15 amp fast-blow IEC 127 fuses (P/N 954745) A.2 Physical Dimensions Height: 56.1 cm (22.1 in) (Without Width: 22.4 cm (8.8 in) reservoir or...
Page 208: Environmental
ICS-2100 Ion Chromatography System A.3 Environmental Operating 4 to 40 °C (40 to 104 °F); cold room-compatible (4 °C) as long Temperature as system power remains on Operating 5% to 95% relative humidity, noncondensing Humidity Operating Pressure 35 MPa (5000 psi) maximum liquid path (tubing, valve, columns, etc.) A.4 Front Panel Power LED...
Page 209 A • Specifications Flow Accuracy <0.1% typical Pressure Ripple <1% at 13.8 MPa (2000 psi) and 1.0 mL/min Eluent On/Off Standard feature Valve Piston Seal Dual-pump head; wash can be continuous when connected to rinse Wash solution supply • Pressure Alarm Upper limit: 0 to 35 MPa or 0 to 5000 psi in one unit (MPa or Limits psi) increments...
Page 210: Eluent Generation
ICS-2100 Ion Chromatography System A.6 Eluent Generation Eluent Types KOH, LiOH, NaOH, K /KHCO , MSA Gradient Profiles Optional feature; combination of unlimited number of linear, convex, and concave positive and negative gradient profiles Concentration 0.01 mM Increments Flow Rate 0.1 to 3.0 mL/min Maximum 21 MPa (3000 psi)
Page 211 A • Specifications EluGen Eluent Concentration Range Comment Cartridges /EPM 0.1 to 15 mM at 0.1 to 1.0 mL/min flow The total of the eluent 0.1 to X mM at 1.0 to 2.0 mL/min flow concentrations from the Electrolytic pH X = 15/flow EluGen cartridge and the Modifier...
Page 212: Detector Electronics
ICS-2100 Ion Chromatography System A.7 Detector Electronics Type Microprocessor-controlled digital signal processor Cell Drive 8 kHz square wave Detector 1% up to 1 mS Linearity Resolution 0.00238 nS/cm • Digital signal range: 0 to 15,000 μS Full-Scale • Output Ranges Analog signal range: 0 to 15,000 μS ±0.1 nS/cm when background conductivity is 0 to 150 μS/cm Electronic Noise...
Page 213 A • Specifications Operating 30 to 55 ºC (86 to 130 ºF); must be at least 7 ºC above the ambient Temperature temperature Doc. 065291-01 3/09...
Page 214: Injection Valve
ICS-2100 Ion Chromatography System A.9 Injection Valve Injection Valve 6-port, 2-position Rheodyne valve with PEEK wetted components; electrically-activated A.10 Auxiliary Valve Optional) Auxiliary Valve 6-port or 10-port, 2-position Rheodyne valve with PEEK wetted components; electrically-activated A.11 Vacuum Degas Assembly Channel Single-channel degas channel with degas membranes Pump Dual-stage diaphragm vacuum pump...
Page 215: Suppressors
A • Specifications A.14 Suppressors Chemical 2 mm and 4 mm anion and cation, membrane suppression bed types Suppression Displacement 2 mm and 4 mm anion and cation, membrane suppression bed types Chemical Regeneration • Electrolytic Self-Regenerating: 2 mm and 4 mm anion and cation; both ™...
Page 216: Autosampler
ICS-2100 Ion Chromatography System SRS 300 (4 mm): <50 μL Void Volume SRS 300 (2 mm): <15 μL MMS 300 (4 mm): <50 μL MMS 300 (2 mm): <15 μL AMMS ICE 300 (4 mm): <50 μL AMMS ICE 300 (2 mm): <15 μL Anion AES: <35 μL Cation AES: <35 μL A.15 Autosampler...
Page 217 A • Specifications System Wellness Standard feature and Predictive Performance Data Trending Numerical device parameters can be plotted Plots • Virtual Column Evaluation mode: Standard feature • Simulator Isocratic and gradient modes: Optional features Application Standard feature Templates Automation Fully controls over 300 different instruments from more than 30 Support for manufacturers, including GC, HPLC, and MS Third-Party...
Page 218 ICS-2100 Ion Chromatography System Storage of Factory, current, and previous calibration values are stored; the user System can reset current values to factory or previous values Calibration Settings Customized Standard feature; unlimited report workbooks Reporting Automated Detailed, comprehensive qualification reports System Qualification Doc.
Page 219: B • Touch Screen Operation
B • Touch Screen Operation The LCD touch screen on the ICS-2100 front panel allows local control of most ICS-2100 functions. This chapter describes how to operate the ICS-2100 from the touch screen. When the ICS-2100 starts up, the touch screen displays a startup page. This is followed by the page, which displays version and serial numbers INFORMATION...
Page 220 ICS-2100 Ion Chromatography System • Edit fields and command buttons have blue text on a lightly shaded background. Fields that display status information are not shaded. • Edit fields have square corners while command buttons are rounded. • To edit a field or select a command button, touch and release the field or button with your fingertip.
Page 221 B • Touch Screen Operation • Touching the page name (for example, ) in the bottom right corner opens a menu of pages (see Figure B-3). Touch a page name to display the page. Figure B-3. ICS-2100 Touch Screen Menu of Pages •...
Page 222: Using The Touch Screen With Chromeleon
ICS-2100 Ion Chromatography System • Touching returns you to the page. HOME • Touching opens a list of options (see Figure B-5). Touch an option to select it. Note: To cancel and retain the current selection, touch outside of the list. Figure B-5.
Page 223: Overview Of Ics-2100 Touch Screen
B • Touch Screen Operation B.3 Overview of ICS-2100 Touch Screen Pages Figure B-6 illustrates the organization of the ICS-2100 touch screen pages. HOME These pages can be PLOT opened from the menu or the Home page STATUS PUMP Diagnostic Pages Calibration Pages EGC1 FLOW RATE...
Page 224: Home Page
ICS-2100 Ion Chromatography System B.4 Home Page Status Field Edit Field Command Button (selected) Command Button (unselected) Figure B-7. ICS-2100 Touch Screen Home Page B.4.1 Home Page Pump Controls Pressure Display page indicates the current pressure reading from HOME the pressure transducer. The pressure unit can be set to psi (the factory default), MPa, or bar (see Section B.7.1).
Page 225 B • Touch Screen Operation Flow Rate The flow rate can be set to 0.00 (which turns off the pump) and to between 0.05 and 5.00 mL/min. The recommended operating flow rate is between 0.40 and 2.00 mL/min. Refer to the column manual for the flow rate to use with your column and application.
Page 226: Home Page Egc Controls
ICS-2100 Ion Chromatography System B.4.2 Home Page EGC Controls controls indicate the EGC-1 Eluent EGC-1 type and the eluent Cartridge Concentration concentration of each installed Type EluGen Cartridge (EGC). The EGC-2 field indicates whether CR-TC EGC-2 Eluent Cartridge Concentration the Continuously Regenerated Type Trap Column is on or off.
Page 227: Home Page Injection Valve Controls
B • Touch Screen Operation B.4.4 Home Page Injection Valve Controls Inject Load buttons select the position of the injection valve. A dark background and white text on a button indicates the current valve position. For details about the valve positions, see Section 2.4.6.
Page 228: Other Home Page Controls
ICS-2100 Ion Chromatography System Suppressor Controls Suppressor controls indicate the suppressor current setting and the type of suppressor installed. To set the suppressor type, change the current setting, or turn the suppressor on or off, touch the suppressor button. page appears (see Section B.9).
Page 229: Plot Page
B • Touch Screen Operation B.5 Plot Page page provides a real-time plot of the detector conductivity output. To PLOT open the page, touch the button on the page, or select from the PLOT HOME PLOT menu of pages. The page can display up to 60 minutes of data. Data older than 60 minutes is discarded.
Page 230: Status Page
ICS-2100 Ion Chromatography System B.6 Status Page page displays the current status of various operating parameters. To STATUS open this page, touch the button on the page or select from STATUS HOME STATUS the menu of pages. Figure B-9. Status Page Status Page Controls •...
Page 231 B • Touch Screen Operation • The fields to the right of the bottle display the status of four other operating parameters. You can choose from eight possible parameters to display here. Figure B-9 shows the default parameters: Eluent time Flow rate EGC conc .
Page 232: Viewing Other Status Parameters
ICS-2100 Ion Chromatography System B.6.1 Viewing Other Status Parameters Follow the steps below to view the status of a parameter that is not currently displayed on the page. STATUS 1. Touch the button. A drop-down list replaces the label for each Setup field, and the button changes to...
Page 233: Status Parameter Details
B • Touch Screen Operation 2. Touch the down arrow next to the field you want to change. A list of the available status parameters appears (see Figure B-12). Figure B-12. Status Page Parameter List 3. Touch the parameter you want to display. 4.
Page 234 ICS-2100 Ion Chromatography System Status Parameter Description EGC1 life The percentage of ions remaining in the EluGen cartridge (EGC1 EGC2 life or EGC2). When the EGC-2 power supply is configured as an auxiliary power supply, the field will display EGC2 life Cell temp The temperature of the cell heater (°C), followed by a status symbol (=, ↑, or ↓).
Page 235: Pump Page
B • Touch Screen Operation B.7 Pump Page Use the page to set parameters related to pump operation, including PUMP pressure limits, pressure unit, vacuum degas pump settings, and eluent valve control. To open this page, select from the menu of pages. The current PUMP system pressure is displayed at the top of the screen.
Page 236: Setting Degas Operating Parameters (Optional)
ICS-2100 Ion Chromatography System • To change the low pressure limit, touch the field and enter the desired limit on the number pad. To select the pressure unit for all touch screen pages that display pressure values, touch the pressure unit field and select the desired unit on the list , or ) (see Figure...
Page 237: Controlling The Eluent Valve
B • Touch Screen Operation 3. Select the desired option: Always Off : The degas pump is always off. : The degas pump is always on. This setting is reserved for Always On test purposes by a Dionex Service Representative. Select the Degas, Always On option only when testing the vacuum degas assembly.
Page 238: Eluent Generator Page
ICS-2100 Ion Chromatography System B.8 Eluent Generator Page Use the page to set the eluent concentration of each ELUENT GENERATOR installed EluGen cartridge (EGC). You can also enter the serial number and view the expiration date and remaining life of each cartridge. This page also provides on/off control of a Continuously Regenerated Trap Column (CR-TC).
Page 239: Setting The Eluent Concentration
B • Touch Screen Operation If the ICS-2100 is not under Chromeleon control, enter the cartridge serial number by touching the field on the Serial Number ELUENT GENERATOR page (see Figure B-16) and entering the serial number on the keypad. If you are reinstalling a cartridge that was used previously, touch the down arrow instead of the number field.
Page 240: Controlling The Eluent Generator Power
ICS-2100 Ion Chromatography System NOTE When the EGC-2 power supply is configured as an auxiliary power supply, the EGC-2 Setpoint fields display zeros. Actual Concentration B.8.3 Controlling the Eluent Generator Power • To manually control the eluent generator power, touch the EGC Off buttons on the page.
Page 241: Controlling The Cr-Tc Power
B • Touch Screen Operation below 30%. At 0%, an error message informs you that the ion count is depleted and the cartridge must be replaced before continuing operation. NOTE When the EGC-2 power supply is configured as an auxiliary power supply, the EGC-2 Life Remaining field is blank.
Page 242: Suppressor Page
ICS-2100 Ion Chromatography System B.9 Suppressor Page Use the page to specify the type of suppressor installed in the ICS- SUPPRESSOR 2100, turn the suppressor on and off, and select the amount of current supplied to the suppressor. To open this page, touch the suppressor button on the page, HOME or select...
Page 243: Detector Page
B • Touch Screen Operation NOTE When Chromeleon is used to control the ICS-2100, the Program Wizard automatically calculates the current required for the application. • To manually control the suppressor power, touch the buttons. • To automatically control the suppressor power simultaneously with the pump flow, select Automatically turn on with pump NOTE The suppressor always turns off when the pump is off,...
Page 244: Setting The Data Rise Time
ICS-2100 Ion Chromatography System B.10.1 Setting the Data Rise Time The data rise time determines the amount of filtering performed on the conductivity data. The rise time is a measure of how quickly the detector responds to a change in signal, and is defined as the time it takes the output signal to rise from 10% of its final value to 90% of its final value.
Page 245: Setting Analog Out Options
B • Touch Screen Operation B.10.3 Setting Analog Out Options The analog output connector on the ICS-2100 rear panel outputs an analog voltage signal proportional to the conductivity measured by the cell. The output is filtered using the selected data rise time (see Section B.10.1).
Page 246: Information Page
ICS-2100 Ion Chromatography System B.11 Information Page page displays the version numbers of the ICS-2100 control INFORMATION programs (Moduleware and Boot Block), the serial number of the ICS-2100, and the options that are installed in the ICS-2100. The page is INFORMATION displayed at start-up.
Page 247: Module Setup Page
B • Touch Screen Operation B.12 Module Setup Page page lets you select options for the touch screen display and MODULE SETUP conductivity readings. To open this page, select from the menu of pages. SETUP Figure B-21. Module Setup Page Module Setup Options •...
Page 248: Input/Output Page
ICS-2100 Ion Chromatography System B.13 Input/Output Page page provides control of the ICS-2100 TTL inputs and TTL INPUT/OUTPUT and relay outputs. To open this page, select from the menu of INPUT/OUTPUT pages. For details about relay and TTL control, see Appendix Figure B-22.
Page 249 B • Touch Screen Operation Figure B-23. System Diagnostic Menu Page NOTE When the EGC-2 power supply is configured as an auxiliary power supply, pressing the EGC2 Verification button has no effect. NOTE The button provides access to service functions Service that are performed only by Dionex personnel.
Page 250 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 251: C • Ttl And Relay Control
C • TTL and Relay Control C.1 TTL and Relay Connections A 12-pin connector strip for TTL/relay control is located on the ICS-2100 rear panel. The connector provides two relay outputs, two TTL outputs, and four TTL inputs (see Figure C-1).
Page 252 ICS-2100 Ion Chromatography System The outputs can be used to control functions in external devices such as an autosampler or another Dionex module. When connected to a controlling device, the inputs can be programmed to perform the following ICS-2100 functions: •...
Page 253 C • TTL and Relay Control b. Plug the connector into the 12-pin connector on the ICS-2100 rear panel. c. Connect the wires from the ICS-2100 connector plug to the TTL or relay connector pins on the other module(s). Additional connector plugs are provided with other Dionex modules.
Page 254: Selecting Ttl Input Functions And Control Types
ICS-2100 Ion Chromatography System C.1.1 Selecting TTL Input Functions and Control Types To select TTL input functions and the control type from Chromeleon, open the Chromeleon Server Configuration program and double-click the ICS-2100 icon under the timebase. Select the TTL Inputs tab (see Figure C-3).
Page 255 C • TTL and Relay Control To select TTL input functions and the control type from the touch screen, go to the touch screen page (see Figure C-4). INPUT/OUTPUT Select a Function Select a Control Type Figure C-4. Input/Output Page: Selecting TTL Functions TTL Input Control Types The ICS-2100 TTL inputs respond to four types of signals to accommodate different controlling devices.
Page 256 ICS-2100 Ion Chromatography System The action of the positive (rising) edge depends on the function: For Load/Inject function, the rising edge switches the injection valve to the Inject position. For the function, the rising edge turns Pump On off the pump (and suppressor). For Autozero Mark , the rising...
Page 257: Controlling Ttl And Relay Outputs
C • TTL and Relay Control C.2 Controlling TTL and Relay Outputs The ICS-2100 provides two TTL outputs and two relay contacts for control of functions in external devices, such as an integrator or autosampler. The relay outputs can be used to switch any low-voltage control. Switched current must be less than 200 mA and 60 V peak blocking.
Page 258: Example Setup For Stand-Alone Operation
ICS-2100 Ion Chromatography System C.3 Example Setup for Stand-Alone Operation This section describes an example setup for stand-alone operation (operation without Chromeleon software) with an AS Autosampler and a third-party analog- to-digital converter and controller. If your configuration includes different devices, use the following steps as guidelines and refer to your product documentation for specific connection details.
Page 259 C • TTL and Relay Control ICS-2100 Rear Panel Connections AS Rear Panel Connections ANALOG OUTPUT TTL 1 IN (+) TTL 2 OUT (+) TTL 2 IN (+) TTL 1 OUT (+) TTL 3 IN (+) RLY 2 OUT TTL 4 IN (+) RELAY RLY 1 OUT IN/OUT (-)
Page 260 ICS-2100 Ion Chromatography System Assign ICS-2100 Input Functions Go to the ICS-2100 touch screen page and verify that the Inject INPUT/OUTPUT function is assigned to TTL input 1, that Autozero is assigned to TTL input 2, and that the TTL Input Control Type Normal Edge (see Figure...
Page 261 C • TTL and Relay Control Connect the Analog Output 1. Locate the analog cable for the third-party analog-to-digital converter and controller. 2. Connect the analog input connector on the third-party controller to the connector on the ICS-2100 rear panel (see Figure C-6).
Page 262 ICS-2100 Ion Chromatography System Timed Event Notes: • At time INIT, the injection valve is set to Load. • At time 0.00, the injection valve is set to Inject, an autozero is performed (TTL2=1), the data acquisition mark is sent (RLY1=1), and the pump is turned on (RLY2=1).
Page 263: D • Reordering Information
D • Reordering Information Part Number Item Reservoirs 046548 2-L plastic reservoir assembly (includes stopper and cap) 039164 4-L plastic reservoir assembly (includes stopper and cap) Pump 057937 Primary pump head assembly 057938 Secondary pump head assembly 045721 Outlet check valve assembly, 10-32 045722 Inlet check valve assembly, 1/4-28 045994...
Page 264 ICS-2100 Ion Chromatography System Part Number Item 058069 EGC holder and degas assembly Sample Loop and Injection Valve 042857 25-μL sample loop 057968 Injection valve 024305 Luer adapter fitting, 1/4-28 (for manual injections) 016388 1-cc syringe (for manual injections) 057896 Injection Valve Rebuild Kit Auxiliary Valve 069472...
Page 265 D • Reordering Information Part Number Item 043598 Twisted pair of wires Miscellaneous 954745 IEC 127 fast-blow fuses, rated 3.15 amps 060494 USB cable, 5 m (16 ft) 060392 External USB hub Preventive Maintenance Kits 057954 ICS-2100 Preventive Maintenance Kit 060581 AS Preventive Maintenance Kit 055647...
Page 266 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 267: E • Faq
E • FAQ How do I hook up an autosampler? For instructions on how to connect the ICS-2100 to an autosampler, refer to the ICS-2100 installation instructions. Also refer to the autosampler operator’s manuals, which are included on the Dionex Reference Library CD-ROM (P/N 053891).
Page 268: When Should I Replace The Eluent Generator Cartridge
ICS-2100 Ion Chromatography System When should I replace the eluent generator cartridge? The ICS-2100 monitors EluGen cartridge use and displays a message when it is time to replace the cartridge. The message appears on the front panel screen and in the Chromeleon Audit Trail.
Page 269: How Do I Know When A Column Is Dirty
E • FAQ E.12 How do I know when a column is dirty? See the troubleshooting section of the column manual. E.13 How do I clean a column? See the troubleshooting section of the column manual. E.14 Why is the conductivity high? Possible reasons for high conductivity include: •...
Page 270 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 271: F • Glossary
F • Glossary Analytical Column Synonymous with Separator Column. Band Spreading The broadening of the sample band as it travels through the column. Band spreading can also occur in the injection valve, detector cell, and interconnecting tubing. Calibration Curve A graph showing detector response in peak height or area versus analyte concentration.
Page 272 ICS-2100 Ion Chromatography System Cell Constant (k) A factor determined experimentally by measuring the conductance (G) of a standard solution of known equivalent conductivity (k). κ G ⁄ The value of k depends upon the surface area of, and distance between, the electrode faces in the conductivity detector cell.
Page 273 F • Glossary of relatively clean sample. This allows large volumes of sample to be injected, lowering concentration detection limits. Conductivity A measure of the ease with which electrical current flows through a liquid contained between two opposite charged electrodes. Conductivity is a characteristic of ions in solution.
Page 274 ICS-2100 Ion Chromatography System Packing The material that fills a chromatographic column; usually a resin or silica-based material. Pellicular Resin A resin with a solid, nonporous core coated with a thin layer of more porous material. The exchange sites of pellicular ion exchange resins are located only on the surface layer of the bead.
Page 275 F • Glossary Siemens (S) Unit measure of conductance; the reciprocal of the electrical resistance of a solution. Suppressor A device used to minimize eluent conductivity and convert sample species to a common form, thus increasing detection sensitivity. Temperature Coefficient The percent of change in the conductivity of a solution with a 1 C change in temperature.
Page 276 ICS-2100 Ion Chromatography System Doc. 065291-01 3/09...
Page 277 Index Symbols Autosampler Loading samples with, 64 % Offset (analog out), 233 See also AS Autosampler See also AS-DV Autosampler Autosampler Control panel, 31 Autosampler specifications Automated dilution, 204 Adjusting the contrast, 12, 207 Automation, 204 Air particulate samples, 61 Automation flexibility, 204 Alarm conditions, 74 –...
Page 278 ICS-2100 Ion Chromatography System Backlight Audit Trail error messages, 71 Time-out, 235 Commands, 66 Backpressure, 36 Control panel, 65 Restriction in plumbing, 109 Degas options, 97 Troubleshooting, 92 Error messages, 74 Backpressure coil, 40 Interface, 30 Backpressure, low Menu, 65 How to increase, 40 Overview, 30 Bar, 224...
Page 279 Index Conductivity cell, 19 – 20, 46 Data Calibrating, 103 Backing up, 256 Calibration constant, 128 Deleting, 256 High cell output, 95 Filtering, 232 Leaking, 87 Saving, 65 – 66 No response, 94 Data acquisition, 65 Replacement procedure, 127 Data analysis description, 3 Setting the temperature, 215 Data rise time, 231 –...
Page 280 ICS-2100 Ion Chromatography System Delivery process, 2 Filtering, 54 EGC, 16 See also Eluent generator Backpressure requirement, 40, 43 Eluent bottle Concentration ranges, 38 See Eluent reservoir Concentration upper limits, 78, 227 Eluent concentration, 38 EGC-1 connector, 16, 148, 156 Eluent generator, 16, 37 EGC-2 connector, 16 Backpressure, 43...
Page 281 Index Column heater open circuit, 76 Carbonate/bicarbonate eluent generation, Column heater short circuit, 76 CR-TC over current error, 76 KOH, LiOH, MSA,NaOH eluent CR-TC stopped for zero flow rate, 77 generation, 25 Degas calibration failed, 77 Flow rate, 19, 196, 213 EGC board not present, 77 Calibration, 106 EGC-1 calibration error, 78...
Page 282 ICS-2100 Ion Chromatography System See also Touch screen Leak sensor, 19 ICS, 52 Leak sensor wet alarm, 80 Information page, 234 Replacement, 137 Injecting samples, 62 Leaks Via autosampler, 64 Auxiliary valve, 87 Via syringe, 63 Cell, 87 Via vacuum syringe, 64 Fittings, 86 Injection port, 11 Injection valve, 87...
Page 283 Index MPa, 224 MSA EGC, 37 Panel tabset, 30 How to display, 31 Panels Chromeleon Control panel, 65 NaOH EGC, 37 Chromeleon Wellness panel, 99 Nitrite in samples, 61 Component panel, 18 Noise, baseline, 232 Parameters Normal edge TTL input control, 243 Chromeleon operating commands, 66 Normal pulse TTL input control, 244 Peak ghosting, 93...
Page 284 ICS-2100 Ion Chromatography System Replacing tubing and fittings, 113 Priming, 57, 138 Troubleshooting, 109 Priming problems, 88 Polarity Secondary pump head, 35 Selecting the conductivity polarity, 232 Selecting the pressure unit, 223 Potassium carbonate eluent, 41 Setting pressure limits, 223 Potassium hydroxide eluent, 37 Stopped due to lost USB communication, Power cord, 142...
Page 285 Index Manual, 50, 65 With Chromeleon, 65 – 66 Range, 217, 233 Samples Rear panel, 21 Collecting and storing, 60 Analog output connector, 21 Diluting, 61 Power receptacle, 22 Filtering, 60 USB connections, 21 Injecting, 62, 64 Rebuilding the auxiliary valve, 114 Loading, 62 Rebuilding the injection valve, 114 Pretreating, 61...
Page 286 ICS-2100 Ion Chromatography System Rebuilding the auxiliary valve, 114 Vacuum degas assembly, 202 Rebuilding the injection valve, 114 Stand-alone operation, 246 – 249 Replacing tubing and fittings, 113 Analog output connection, 249 Suppressor replacement, 130 AS setup, 248 – 249 Tubing and fittings replacement, 113 Example connections, 247 Waste valve O-ring replacement, 125...
Page 287 Index No cell response, 94 Peak ghosting, 93 Technical Support, 71, 99 Peak height, 94 Temperature Pump, 88 – 90 Cell heater, 46, 215 Retention time, 94 Column heater, 20, 45, 214 Selectivity, 94 Minimizing the effect of variations, 46 Vacuum degas assembly, 97 Temperature compensation, 46 TTL and relay connector, 239...
Page 288 ICS-2100 Ion Chromatography System See Waste valve Version number, 234 Warranty, voiding, 99, 114, 116 Waste container, 185 Waste lines Blocked, 86 Gas separator waste tube, 37 Waste valve, 35 Opening, 35, 120 O-ring replacement procedure, 125 Water samples, 61 Weekly maintenance, 68 Weight, 195 Wellness, 31...

Dionex ICS-2100 Operator's Manual

Table of Contents

1 Introduction

2 Features

3 Operation and Maintenance

4 Troubleshooting

Ics-2100 Ion Chromatography System

5 Service

A • Specifications

B • Touch Screen Operation

C • TTL and Relay Control

D • Reordering Information

E • Faq

F • Glossary

Quick Links

Chapters

Troubleshooting

Need help?

Questions and answers

Related Manuals for Dionex ICS-2100

Summary of Contents for Dionex ICS-2100