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Hitachi CR22GIII Instruction Manual
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Hitachi CR22GIII Instruction Manual

Hitachi CR22GIII Instruction Manual

High-speed refrigerated centrifuges
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INSTRUCTION MANUAL
HITACHI
HIGH-SPEED REFRIGERATED CENTRIFUGES
CR22GⅢ/CR21GⅢ
Thank you for purchasing the Hitachi
high-speed refrigerated centrifuge.
Before using this centrifuge,
carefully read through
this instruction manual to ensure
efficient and safe operation.
Keep this instruction manual handy.
・
The appearance or specification of the products covered in this manual is
subject to partial change for improvement.
July 2009
S99807002

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  • Page 1 INSTRUCTION MANUAL HITACHI HIGH-SPEED REFRIGERATED CENTRIFUGES CR22GⅢ/CR21GⅢ Thank you for purchasing the Hitachi high-speed refrigerated centrifuge. Before using this centrifuge, carefully read through this instruction manual to ensure efficient and safe operation. Keep this instruction manual handy. ・ The appearance or specification of the products covered in this manual is subject to partial change for improvement.
  • Page 3 "NOTE" indicates a note which has no direct bearing on personal safety. Do not perform any operation not specified in the instruction manual. If any problem is found on your instrument, contact a Hitachi Koki authorized sales/service representative. Although the safety precautions in the instruction manual and safety instructions on your instrument have been fully considered, an unexpected situation may arise.
  • Page 4 Be sure to set all the buckets of the same serial number. If abnormal sound or vibration occurs, stop the operation immediately and ● contact a Hitachi Koki authorized sales/service representative. CAUTION： Before using a rotor, be sure to read through the rotor instruction manual.
  • Page 5 Hitachi Koki authorized sales/service representative. Notice for an Earthquake Depending on the magnitude, an earthquake might damage the centrifuge. If you observe some abnormality, stop using the centrifuge immediately and ask for inspection by the Hitachi Koki service representative.
  • Page 6 Table of Contents 1. Specifications ･････････････････････････････････････････････････････････ 2. Structure ･････････････････････････････････････････････････････････････ 2-1 Appearance ･･････････････････････････････････････････････････････････････････････ 2-1 2-2 Structure ････････････････････････････････････････････････････････････････････････ 2-2 2-2-1 Operation Panel ･･････････････････････････････････････････････････････････････ 2-2 2-2-2 POWER Switch ･･････････････････････････････････････････････････････････････ 2-6 2-2-3 Safety Device･････････････････････････････････････････････････････････････････ 2-6 3. Operation ････････････････････････････････････････････････････････････ 3-1 Preparation for Operation ･･････････････････････････････････････････････････････････ 3-2 3-1-1 ROTOR ･･････････････････････････････････････････････････････････････････････...
  • Page 7 Table of Contents 5. Troubleshooting ･･････････････････････････････････････････････････････････････････ 5-1 5-1 Alarm Messages ･･････････････････････････････････････････････････････････････････ 5-2 (1) Displaying for alarm information ･････････････････････････････････････････････････ 5-2 (2) Corrective actions ･････････････････････････････････････････････････････････････ 5-3 5-2 User-corrected Problems ･･････････････････････････････････････････････････････････ 5-4 6. Installation and Relocation ･････････････････････････････････････････････ 7. Warranty ･･････････････････････････････････････････････････････････････ 7-1 Warranty on Centrifuge ････････････････････････････････････････････････････････････ 7-1 7-2 Warranty on Rotor ････････････････････････････････････････････････････････････････...
  • Page 8 1. Specifications CR22GⅢ CR21GⅢ Maximum speed 22,000 rpm 21,000 rpm 55,200×g 50,300×g Maximum RCF （R22A4 rotor） （R22A4 rotor） Maximum capacity 4,000ml（R9A rotor） Set speed 300 to 22,000rpm 300 to 21,000rpm Set temperature -20º C to +40º C 1 second to 99 minutes and 59 seconds; HOLD for continuous Set time operation (Option: 1 minute to 99 hours and 59 minutes)
  • Page 9 *1 The following are required to satisfy the CE Marking requirements for the use of the CR 22G Ⅲ / CR 21G Ⅲ centrifuges. (1) Only the rotors with overspeed adapters (magnets) must be used. The CR22G Ⅲ / CR21G Ⅲ refrigerated centrifuge do not conform to the CE marking requirements when using a continuous flow rotor due to the construction of the rotor.
  • Page 10 2-2 Structure 2-2-1 Operation Panel When power switch is turned on, the initial screen is displayed as shown in Fig.2-2-1. When the lockout system is used in the CR22GⅢ or the CR21Ⅲ centrifuge, the initial screen is displayed first and then the initial screen of the lockout system is displayed. For the lockout system, refer to Section 3-3-6.
  • Page 11 (2) Display panel of CR22 GⅢ(When the lockout system has not been set) Fig. 2-2-4 Display panel of CR22 GⅢ(When the lockout system has not been set) (3) Display panel of CR21 GⅢ(When the lockout system has been set) Fig. 2-2-5 Display panel of CR21 GⅢ(When the lockout system has been set) (4) Display panel of CR21 GⅢ(When the lockout system has not been set) Fig.
  • Page 12 [Functions of display panel] Name Function Field display Displays the following run conditions. For SPEED, TIME and TEMP displays, the upper line shows the run state and the lower line shows the set value. Refer to "3-2-1 Setting Run Conditions" for details. ...
  • Page 13 [Function keys] 8. ESC key 11.Ten-key numerical pad 9. MENU key 10. Cursor keys 7. ROTOR key 6. Stop key 5. START key Fig. 2-2-7 Function keys Name Function START key Starts the centrifuge run. STOP key Stops the centrifuge run. ROTOR key Used to display rotor list or to enter desired rotor number.
  • Page 14 2-2-2 POWER Switch POWER switch The POWER switch applies electric power to the centrifuge. 「｜」：ON 「○」：OFF Fig. 2-2-8 POWER switch CAUTION: Always keep the liquid crystal panel in a visible position while the POWER switch is turned on so that you can check the current operating state (running or stopping). 2-2-3 Safety Device (1) Protector of rotor chamber...
  • Page 15 3. Operation The centrifuge operates in a variety of ways so that it may be applied for a wide range of use. A brief description of each mode of operation is given below. Brief description Reference Section 3-2 "Basic Operation" Normal operation Time You can save set run conditions in memory...
  • Page 16 CAUTION ： 1.Read the rotor instruction manual thoroughly before use. 2.Never use any adapter, tube or bottle that is not designated for the centrifuge by Hitachi Koki. 3.Mount the rotor cover securely.
  • Page 17 2. Do not press the function keys with a sharp-pointed object such as a ball-point pen. 3. If abnormal sound is heard during the operation, stop the operation immediately and contact a Hitachi Koki authorized sales/service representative. 3-2-1 Setting Run Conditions This section explains how to set run conditions on the RUN SCREEN and how to use the cursor keys.
  • Page 18 [Cursor key] A cursor appears and blinks on the entry line of a run condition display by pressing a cursor key as shown in Fig. 3-2-2 (2). The entry line state varies depending on the presence of cursor as shown below. (1) Fixed-entry state: No cursor appears in normal state.
  • Page 19 Refer to the next page for setting run conditions (example). NOTE (1) If incorrect value is entered, press the CE key to return to ready-to-enter state. If the incorrect value is already fixed by pressing the ENTER key, press a cursor key to turn to ready-to- enter state and then enter the correct value.
  • Page 20 * Setting run conditions The table below exemplifies how to set run conditions such as rotor speed, run time and rotor temperature. Item Speed （SPEED） Run time （TIME） 22,000rpm 2 minutes , 30seconds Example set value Press a cursor to turn the The display turns to ready-to- The display turns to ready- display to ready-to-enter...
  • Page 21 Temperature Acceleration rate Deceleration （TEMP） （ACCEL） （DECEL） 4℃ The display turns to ready-to- The display turns to ready-to- The display turns to ready-to- enter state. enter state. enter state. The cursor is blinking at the one place. ４ ９ ７ Enter "0"...
  • Page 22 3-2-2 Operating Procedure This section describes the procedure for normal operation. NOTE Before following the procedure, read the rotor instruction manual carefully and make sure that you have selected the appropriate type of tube for the sample, and that the amount of sample in the tubes is correct.
  • Page 23 The RUN mode indicator is displayed on the panel as follows: Time Fig.3-2-3 RUN mode indicater 図 3-2-3 運転モード表示 3-2-3 Acceleration Rate and Deceleration Rate The acceleration and deceleration rates can be adjusted for a wide range of use. The figure below shows how a rotor accelerates and decelerates in compliance with a code number selected from among 1 through 9.
  • Page 24 3-3 FUNCTION Field The CR22GⅢ/ CR21GⅢrefrigerated centrifuge has many add-on features such as programmed operation and centrifugal force values displaying and setting. These features are displayed and selected on the FUNCTION field. Fig. 3-3-1 FUNCTION field PROG : You can save run conditions in memory for later use in repeated operation. This feature also allows step-mode operation (three normal operations can be combined in a sequence of operations).
  • Page 25 (1) Saving and changing run conditions To save or change run conditions in memory, use the following procedure. Step Key operation Screen display and notices Move the cursor to PROG and press the ENTER key. The FUNCTION field turns to the PROGRAM field.
  • Page 26 Step Key operation Screen display and notices  The PROGRAM field appears by the first press of the After saving run conditions, press the ESC key twice. ESC key.  The FUNCTION field appears by the second press of the ESC key. NOTE (1) When the saved run conditions are changed, the previous run conditions are cleared and the newly saved run conditions are in effect.
  • Page 27 (2) Using programmed operation To recall the saved run conditions and use programmed operation with the recalled run conditions, take the following procedure. Step Key operation Screen display and notices Move the cursor to PROG and press the ENTER key. The FUNCTION field turns to the PROGRAM field.
  • Page 28 (3) Procedure for deleting run conditions To delete run conditions saved in memory, take the following procedure. NOTE Check that the rotor stops completely before deleting saved run conditions. Step Key operation Screen display and notices Move the cursor to PROG The FUNCTION field and press the ENTER key.
  • Page 29 3-3-2 Step-mode Operation This centrifuge has the step-mode operation capability that allows you to save three different sets of values for a run parameter set in a single memory location (MEMORY Nos. 31 - 33, 41 - 43, and 51 - 53) and then change some or all of the run conditions (e.g., speed, run time, rotor temperature, etc.) for each step during a step-mode run.
  • Page 30 3-3-3 RTC (Real Time Control) Operation The CR22G Ⅲ / CR21G Ⅲ refrigerated centrifuge can be programmed to perform automatic centrifugation by setting the incorporated time clock to start and end centrifugation at the desired time in advance. This is the RTC (Real Time Control) operation. Fig. 3-3-3 illustrates an example of procedure for RTC operation.
  • Page 31 (1) Procedure for RTC operation Step Key operation Screen display and notices  Set the run conditions referring to "3-2-1 Setting Run Set the run conditions. Conditions".  For time setting, do not select HOLD but enter a numeric value. Move the cursor to ...
  • Page 32 Step Key operation Screen display and notices Enter the desired date and time (month, day, hour and minutes) using the cursor keys and the ten-key numerical pad. Press the ENTER key.  The range for "hour" setting is from 0 to 23 (24-hour display).
  • Page 33 NOTE (1) The RUN mode indicator on the panel turns as follows in RTC operation. Press START key. Time Start automatically Fig. 3-3-4 Operating mode (RTC) (2) Note that the RTC setting is not available in the following cases: 1. The time setting on the RUN SCREEN is "HOLD" (continuous run). Change the run time setting from "HOLD"...
  • Page 34 3-3-4 Displaying and Setting RCF The CR22GⅢ/ CR21GⅢ refrigerated centrifuge retains, in its internal memory, data representing the maximum radii of all available rotors. Based on this data, the centrifuge automatically computes relative centrifugal force (RCF) values from set speed, or speed from set RCF values, and then display the result of computation on the control panel.
  • Page 35 Step Key operation Screen display and notices Move the cursor to RCF(g)  Entered values are and set the desired RCF and displayed on the sample height values. RCF(g) SET column and the SAMPLE (Example) HEIGHT column RCF(g) ＝15300 g respectively.
  • Page 36 3-3-5 ・ Displaying and Setting g This section explains how to use the g ・ sec displaying and setting capability of the centrifuge. (1) Procedure for displaying and setting g ・ sec values Step Key operation Screen display and notices ・...
  • Page 37 3-3-6 Lockout system function The lockout system function is specifically designed for the CR22GⅢ/ CR21GⅢcentrifuge. This lockout system is used to limit the users of the centrifuge. The registered users can use this system after the system administrators have registered the users. It is necessary for the system administrators to read the separated manual “CR22GⅢ/ CR21GⅢ...
  • Page 38 3-3-7 Variable deceleration slope function (CR22GⅢ only） The variable deceleration slope function is specifically designed for the CR22GⅢ centrifuge. Two slower deceleration slopes are selectable in addition to the same slope as the well-reputed slope of the conventional centrifuges. This function is effective for separation of samples that are apt to be disturbed.
  • Page 39 (1) Procedure for calling up the variable deceleration slope function Step Key entry Display on the screen and remarks Move the cursor to DEC.SLOPE with the cursor key and press the “ENTER” key. The current The FUNCTION field setting is displayed. turns to the DEC.SLOPE field.
  • Page 40 2. Never attempt to slow or stop the rotor by hand. CAUTION: Do not perform any operation not specified in this manual. If any problem is found on your centrifuge, contact a Hitachi Koki authorized sales/service representative. (1) Rotation of rotor The rotating rotor coasts free and finally stops if a power failure occurs during operation.
  • Page 41 1. Check that the rotor stops completely. 2. Turn off the POWER switch of the centrifuge and the distribution board of your centrifuge room. 3. Remove the two screws from the lower portion of the front cover. Remove the front cover by pulling the lower portion of the front cover forward and downward.
  • Page 42 3-5 Features on Menu Screen Press the MENU key and a menu appears as follows. (1) User customization (2) Entry of new rotor (3) Alarm information (4)Lock out system Select the desired item with the numeric key and press the ENTER key to show the corresponding screen.
  • Page 43 (1) SCREEN UTILITIES You can customize the centrifuge in display language, current time setting, screen contrast level, etc. 1) Display language (Japanese or English) Select the desired display language either Japanese or English with the numeric key and press the ENTER key.
  • Page 44 (2) FINISH SCREEN If you choose the “2.FINISH SCREEN”, the “FINISH” (see Fig 3-5-6) appears when the rotor stops, and then “FINISH” blinks. If you select “1.NORMAL” , the “FINISH” does not appear when the rotor stops. If you press any function key or open the door while “FINISH” blinks, the RUN SCREEN reappears. Fig.
  • Page 45 CAUTION：Using a cleaning or sterilization method other than the ones recommended in this instruction manual might cause corrosion or deterioration of the centrifuge. Refer to the chemical resistance chart provided with the rotor, or contact Hitachi Koki. CAUTION：Be sure to turn off the POWER switch before cleaning or sterilizing the centrifuge.
  • Page 46 4-4 Rotor (1) To prevent corrosion, take out the rotor from the rotor chamber after operation and remove the rotor cover to dry the tube holes. (2) If any sample is spilt inside the rotor, wash and dry the rotor well, then apply silicone grease lightly to the rotor.
  • Page 47 Hitachi Koki may ask you about the treatment for the centrifuge, rotor or the part if the decontamination is checked and judged as insufficient by Hitachi Koki. It is your responsibility to bear the cost of sterilization or decontamination.
  • Page 48 The CR22GⅢ/ CR21GⅢ refrigerated centrifuge has a self-diagnosis capability that identifies and reports a problem that occurs when the instrument is starting up or in operation, and that affects the operation of the instrument. 5-1 Alarm Messages When a problem occurs that affects instrument operation, the centrifuge beeps and displays corresponding alarm message, in order to report the occurrence of the problem.
  • Page 49 If any of the alarm messages E10 to E95 lights up, it is indicating that the centrifuge has a problem and requires maintenance by Hitachi Koki service representative. When you call the service personnel, tell them the displayed alarm code.
  • Page 50 Keep the cap on. Be careful not to lose it. Drain hose Installation or relocation of your centrifuge must be done by the authorized Hitachi Koki service representative. Contact a Hitachi Koki authorized sales/service representative.
  • Page 51 The centrifuge main body is warranted for one year from the delivery date on condition that it is properly operated and maintained. 7-2 Warranty on Rotor For information on the warranty on rotors, refer to the instruction manuals of each rotor for Hitachi high-speed refrigerated centrifuge. 〔Incidental conditions〕...
  • Page 52 Note that some neutral detergents are still higher than pH9 even if diluted according to the instruction in the maker’s catalog. Use detergents between pH7 and pH9 (The Hitachi cleaning set with a brush (Part No. S305166A) is recommended for cleaning rotors.).
  • Page 53 Be sure to decontaminate the product according to good laboratory procedures and methods, and fill out this Decontamination Sheet and attach it to the product to be returned to Hitachi Koki for repair. Attention: Hitachi Koki Co., Ltd.
  • Page 54 MEMO...
  • Page 55 After-sales Service Periodic inspection of the centrifuge is recommended to assure safe and efficient operation. If the centrifuge fails to function normally, do not attempt to repair it yourself. Contact a Hitachi Koki authorized sales/service representative. Export office Manufacturer 1060, Takeda, Hitachinaka City Head Office: Ibaraki Pref., 312-8502 Japan...

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