Microm HM 505 E Instruction Manual page 57

PART 4 WORKING WITH THE
VACUTOME
4-1 PREPARING THE
VACUTOME
First insert the blade carrier onto the
dovetail guide as already described in part
2-1 and clamp it with the clamping lever.
Select a clearance angle of approx. 28 -
30°.
Insert the blade onto the blade carrier. Put
it on the magnetic surface of the magnetic
disposable blade carrier MV from the side.
NOTE! To move the blade sideways, press
the blade slider downwards so that the
blade is properly put against the ledge of
the blade carrier.
When using the disposable blade carrier
EV, insert the blade between front and rear
clamping plate and tighten it via the
clamping lever on the right side of the
instrument.
Clamp the specimen stage together with
the frozen specimen into the specimen
holder and tighten it via the orienting lever.
After having adjusted the specimen, set the
suction window limits.
Please note that the lower limit of the
cutting window corresponds with the upper
edge of the specimen.
4-2
With the clamping lever loose, you have
the possibility to adjust the specimen
parallel in relation to the cutting edge via
the orienting lever.
(See part 2-9-2 and part 4-4, Instruction
Manual of Microtome Cryostat HM 505 N
or HM 505 E).
While trimming, determine the correct
force of the vacuum by continued cutting
and varying it via the operating knob,
which is placed above the handwheel.
The setting is optimal, when the last
section remains stretched on the blade,
while the penultimate section gets into the
suction slot when starting a new cut.
MICROM International GmbH
Robert- Bosch-Str. 49
D- 69190 Walldorf
386240 - English
Microtome Cryostat HM 505 E
ORIENTATION AND
TRIMMING OF SPECIMENS
57