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For Research Use Only
bdbiosciences.com
23-11093-00 Rev. A
3/2010
Becton, Dickinson and Company
BD Biosciences
San Jose, CA 95131
Tel 877.232.8995
Fax 800.325.9637
ResearchApplications@bd.com
BD LSRFortessa
Cell Analyzer
User's Guide
BD Biosciences
European Customer Support
Tel 32.2.400.98.95
Fax 32.2.401.70.94
help.biosciences@europe.bd.com
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Table of Contents
Related Manuals for BD LSRFortessa
Summary of Contents for BD LSRFortessa
- Page 1 BD LSRFortessa Cell Analyzer User’s Guide For Research Use Only bdbiosciences.com 23-11093-00 Rev. A 3/2010 Becton, Dickinson and Company BD Biosciences BD Biosciences European Customer Support San Jose, CA 95131 Tel 32.2.400.98.95 Tel 877.232.8995 Fax 32.2.401.70.94 Fax 800.325.9637 help.biosciences@europe.bd.com ResearchApplications@bd.com...
- Page 2 BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2010 BD Patents The BD LSRFortessa™ Cell Analyzer is covered by one or more of the following US Patents and foreign equivalents: 7,129,505; 6,944,338; 6,809,804; 6,683,314; 6,014,904 APC-Cy7: US 5,714,386 BD FACS lysing solution: US 4,654,312;...
- Page 3 FCC information WARNING: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment. NOTICE: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
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Page 5: Table Of Contents
Conventions used in this guide ........3 About the BD LSRFortessa documentation ......5 Technical assistance . - Page 6 BD LSRFortessa Cell Analyzer User’s Guide Chapter 4: Maintenance Maintenance overview ..........40 Cleaning the fluidics .
- Page 7 Contents Chapter 8: Troubleshooting Cytometer troubleshooting ........116 Electronics troubleshooting .
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Page 9: Chapter 1: About This Guide
About this guide This section includes these topics: What this guide covers (page 2) Conventions used in this guide (page 3) About the BD LSRFortessa documentation (page 5) Technical assistance (page 7) -
Page 10: What This Guide Covers
BD LSRFortessa Cell Analyzer User’s Guide What this guide covers This guide describes the procedures necessary to operate and maintain your BD LSRFortessa™ cell analyzer. Because many cytometer functions are controlled by BD FACSDiva™ software, this guide also contains information about software features required for basic cytometer setup and operation. -
Page 11: Conventions Used In This Guide
Chapter 1: About this guide Conventions used in this guide About this topic This topic describes the conventions used throughout this guide. These safety symbols are used in this guide and on safety Safety symbols labels to alert you to potential hazards. Symbol Meaning Caution alert... - Page 12 BD LSRFortessa Cell Analyzer User’s Guide Text and keyboard conventions Convention Notes A note describes important features or instructions. Bold Bold is used to indicate software elements such as dialogs, fields, and buttons. Italics Italics are used to highlight book titles.
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Page 13: About The Bd Lsrfortessa Documentation
The online help installed with BD FACSDiva software includes all content from this guide and the documents listed below. Access BD LSRFortessa online help from the Help menu in BD FACSDiva software. The online help is compiled from the following documents:... - Page 14 BD LSRFortessa Cell Analyzer User’s Guide BD High Throughput Sampler User’s Guide: Describes how to set up and operate the BD™ High Throughput Sampler (HTS) option. It also contains a description of BD FACSDiva software features specific to the HTS.
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Page 15: Technical Assistance
When contacting BD Biosciences, have the following information available: Product name, part number, and serial number Version of BD FACSDiva software you are using Any error messages Details of recent system performance For cytometer support from within the US, call 877.232.8995. - Page 16 Need more information fast? Information about this product is also available in your software's Help system. You can keep the Help window open while you use the software or print the information directly from the window. Internet access is not required to use the Help system.
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Page 17: Chapter 2: Introduction
Introduction The following topics are covered in this section: Instrument overview (page 10) Components (page 11) Fluidics (page 13) Sheath and waste containers (page 17) Optics (page 18) Workstation (page 21) - Page 18 BD LSRFortessa Cell Analyzer User’s Guide Instrument overview The BD LSRFortessa is an air-cooled multi-laser benchtop flow cytometer with the ability to acquire parameters for a large number of colors. It uses fixed-alignment lasers that transmit light through a flow cell to configurable octagon and trigon detector arrays.
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Page 19: Components
Chapter 2: Introduction Components About this topic This topic describes the instrument’s components. Instrument overview Heat ventilation slots Power switch (right side) Sample injection port (SIP) fluidics interconnects Front doors (trigon detector arrays) Side drawer (octagon or trigon Control panel detector arrays) Caution! Do not place any objects on top of the instrument. -
Page 20: Fluidics
BD LSRFortessa Cell Analyzer User’s Guide The power switch is located on the right side of the Power switch instrument. Control panel The control panel contains the following fluidics controls: Sample flow rate control buttons Fluid control buttons Sample fine adjust knob...
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