Adjusting Decluster Parameters For Clumpy Cells - Nexcelom Bioscience Cellometer Auto T4 User Manual

Chapter 9. Cell Types
CHO cell count results were analyzed using different "Sensitivity" settings. Figure (a) shows CHO cell image while figures (b) –
(e) show an incremental increase in "Sensitivity" parameter from 1 to 4, respectively. Green outlines indicate live cells while
red outlines indicate dead cells. Both live/dead cell counts in figure (e) agree with visual observation of image in (a).

Adjusting Decluster Parameters for Clumpy Cells

The Decluster Edge Factor and Decluster Th Factor
(Decluster Threshold Factor) parameters may be used to
separate clumpy cells (i.e., connected cells for which it is
difficult to distinguish clearly defined edges). Default
values for these parameters are 0.5 and 1.0, respectively.
For the majority of cell types, the default values for
these parameters are adequate to separate clumpy cells.
However, some cell types (such as MCF7 and PC12)
contain cells having dim boundaries within the clump. In
these cases, decluster parameters can be modified to
visually separate clumpy cells in the captured images.
To define edges of clumpy cells in more detail, slowly
increase the Decluster Edge Factor and Decluster Th
Factor values. These parameters work in conjunction with
the Contrast Enhancement value.
8001009 Cellometer® Auto T4 User Manual Rev C 45