NEW BRUNSWICK SCIENTIFIC Excella E-25 Manual To Operations
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NEW BRUNSWICK SCIENTIFIC Excella E-25 Manual To Operations

Classic incubator shakers
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Excella™ E-25/25R Classic
Incubator Shakers
MANUAL NO: M1353-0050
Revision A
April 24, 2006
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005
Telephone: 1-732-287-1200 • 1-800-631-5417
Fax: 732-287-4222 • Telex: 4753012 NBSCO
Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com

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  • Page 1 Incubator Shakers MANUAL NO: M1353-0050 Revision A April 24, 2006 NEW BRUNSWICK SCIENTIFIC CO., INC. BOX 4005 • 44 TALMADGE ROAD • EDISON, NJ 08818-4005 Telephone: 1-732-287-1200 • 1-800-631-5417 Fax: 732-287-4222 • Telex: 4753012 NBSCO Internet: http://www.nbsc.com • E-mail: bioinfo@nbsc.com...
  • Page 2 M1353-0050 Excella™ E-25/E-25R Classic Incubator Shakers User’s Guide...
  • Page 3 Fax: 49 (0)7022 32486 E-mail: sales@nbsnv-sa.be E-mail: sales@nbsgmbh.de CHINA THE NETHERLANDS New Brunswick Scientific Co., Inc. New Brunswick Scientific BV A903, 904 Yin Hai Building Kerkenbos 1101, 6546 BC Nijmegen No. 250, Cao Xi Road P.O Box 6826, 6503 GH Nijmegen Shanghai 200235, P.R.
  • Page 4 WARNING! This equipment must be operated as described in this manual. If operational guidelines are not followed, equipment damage and personal injury can occur. Please read entire User’s Guide before attempting to use this unit. M1353-0050 Excella™ E-25/E-25R Classic Incubator Shakers User’s Guide...
  • Page 5 Reproduction, adaptation, or translation without prior written permission from New Brunswick Scientific is prohibited. Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
  • Page 6 W A R R A N T Y Every Instrument manufactured by the New Brunswick Scientific Co., Inc. is warranted to be free from defects in material and workmanship. This apparatus, with the exception of glassware, lamps and electrodes (where supplied), is warranted against faulty components and assembly for 2 years in the United States &...

  • Page 7: Table Of Contents

    UNCTIONS .......................27 LARM UNCTIONS ....................27 EMPERATURE ETPOINT ................28 EMPERATURE FFSET ALIBRATION ......................29 OWER AILURE .....................29 PEED ALIBRATION PREVENTIVE MAINTENANCE..................30 ..................30 LEANING XTERNAL URFACES .....................30 EPLACEMENT .....................33 EPLACEMENT TROUBLESHOOTING ....................35 REPLACEMENT PARTS & ACCESSORIES...............37 New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 8 ....................37 EPLACEMENT ARTS ........................37 CCESSORIES DRAWINGS ........................39 10.1 .......................44 IST OF RAWINGS 10.2 ......................45 IST OF ABLES INDEX..........................46 M1353-0050 Excella™ E-25/E-25R Classic Incubator Shakers User’s Guide...

  • Page 9: Overview

    For safe operation, both the E-25 and E-25R are designed with a safety switch that automatically stops the shaker mechanism when the lid is lifted. In addition, the Excella E-25 and E-25R are equipped with visual and audible alarms that alert the user to the following conditions: •...

  • Page 10: Specifications

    1.1 Specifications Excella E-25 & E-25R Incubator Shaker SHAKING 50-400 rpm Speed ± 1 rpm Control Accuracy 3-digit LED, displayed in 1 rpm increments Indication 1 inch (2.54 cm) Stroke/Orbit TEMPERATURE 7°C above ambient temperature* to 60°C Range (E-25) 15°C below ambient temperature* (min. 4°C) to 60°C Range (E-25R) ±...

  • Page 11: Certifications

    1.2 Certifications The Excella E-25 & E-25R have been tested to ETL standards, to comply with UL and CAN/CSA electrical safety standards (see “ETL Regulatory Standards” in the specifications table, Section 1.1). As attested in the CE Declaration of Conformity reproduced on the following page, they also conform to the appropriate CE standards (see also “CE Regulatory Standards”...
  • Page 12 M1353-0050 Excella™ E-25/E-25R Classic Incubator Shakers User’s Guide...

  • Page 13: Inspection, Verification & Unpacking Of Equipment

    (both of which should be the same). NOTE: Use of the Excella E-25/25R Shakers requires a platform, which is a separate item. See the Available Platforms list in Section 9.2. New Brunswick Scientific Co., Inc.

  • Page 14: Out Of Box Concerns

    2.4 Out of Box Concerns If any part of your order was damaged during shipping, is missing pieces, or fails to operate properly, please contact your sales representative and also fill out Customer Satisfaction Form 6300, included with your equipment, and return it by fax. 2.5 Warranty Registration Please complete and return your warranty card or register electronically at our Website: www.nbsc.com...

  • Page 15: Preparing The Location

    The E-25R shakers can be equipped to run on: 100 Volts, 50/60 Hz, 1500 VA maximum 120 Volts, 60 Hz, 1500 VA maximum 230 Volts, 50 Hz, 1500 VA maximum In all cases, voltage variation must not exceed ± 10%. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 16: Space Requirements

    3.4 Space Requirements Figure 1: Space Requirements Height with lid open: 65¼ inches (165.7 cm) 4 inches 34½ inches (10 cm) front (87.6 cm) front to back & left side clearance clearance 50 inches (127 cm) 24 inches (61 cm) for access to service door M1353-0050 Excella™...

  • Page 17: Features

    E-10. This section will acquaint you with the keypad’s user interface keys, LED display, status indicators and function indicators. Figure 2: Front Panel KEYPAD ON/OFF SWITCH Figure 2a: Keypad STATUS DISPLAY INDICATORS FUNCTION INDICATORS USER INTERFACE KEYS New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 18 4.1.1 LED Display The digital display on the control panel is a three-digit . During LED DISPLAY normal shaker operation, the display will indicate: • Shaker status (On/Off) • Shaking speed • Chamber temperature • Setpoints • Hours remaining (in a timed run) •...
  • Page 19 START/STOP again. When a timed run ends, the indicator will blink. Press the key and change to SELECT another function to turn off this indicator. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 20 • This feature is activated by the key. SELECT MUTE When activated, the audible alarm is muted, and remains so until is is reactivated with the key. If is activated when the SELECT MUTE shaker is turned off using the switch, it ON/OFF will remain engaged when the machine is...

  • Page 21: Getting Started

    (see Figure 3). Figure 3: Platform Screw Locations PLATFORM SCREWS PLATFORM SCREWS 2. Place the platform on the upper bearing housing, aligning the mounting holes of the platform with the platform screw locations. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 22: Installing Flask Clamps

    3. Insert the four platform screws previously removed and, using the hex wrench, tighten them to secure platform. 5.2 Installing Flask Clamps Flask clamps purchased for use with universal platforms (see Section 9.2) require installation. Clamps are installed by securing the base of the clamp to the platform with the correct type and number of screws.

  • Page 23: Electrical Connections

    ON/OFF SWITCH shaker (see Figure 2), is in the position. Connect the to the on the rear panel (see Figure 5) POWER CORD POWER CONNECTOR and the other end to a suitable, grounded receptacle. New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 24 Figure 5: E-25 and E-25R Rear Panel POWER CONNECTOR ELECTRICAL SPECIFICATION PLATE M1353-0050 Excella™ E-25/E-25R Classic Incubator Shakers User’s Guide...

  • Page 25: Operation

    3. Press either to enter (the will illuminate). SET MODE SET INDICATOR 4. Set the speed by using the until the desired setpoint is displayed. Holding the will cause the setting to change more rapidly. New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 26: Checking A Setpoint

    NOTE: The setpoint may be changed during a run without stopping the shaker by following steps 1-4 above. 6.3 Checking a Setpoint 1. Press until the desired indicator is illuminated. SELECT 2. Press either to enter the and display the current setpoint. SET MODE CAUTION! Holding the...

  • Page 27: Alarm Functions

    START/STOP KEY Alarm Functions The Excella E-25 and E-25R shakers have an audible alarm that is activated at predetermined times. It can be deactivated by using the function: MUTE 1.

  • Page 28: Temperature Offset Calibration

    1. To deactivate the temperature control system, press and hold the until the setpoint is at 4.0°C. START / STOP KEY 2. Holding the , press the 3. The temperature setpoint display shows and the control system will be deactivated. To reactivate the temperature controller, press the until the desired temperature setpoint is displayed.

  • Page 29: Power Failure

    3. Press either the to change the displayed value to match the measured speed. KEYS 4. Press the simultaneously to save the adjustment. 5. Turn unit using the power switch, then turn it back New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 30: Preventive Maintenance

    7.2 Fuse Replacement Excella E-25 models require one electrical fuse, which is housed in the fuse holder located on the M1324-7004 PCB (see Figure 7a). E-25R models have two electrical fuses, housed in the two fuse holders that are located on the M1324-7003 PCB (see Figure 7b).
  • Page 31 Figures 7a (E-25) and 7b (E-25R). Figure 7a: Rear of Electrical Panel (E-25) FUSE HOLDER (see Figure 7c for details) Figure 7b: Rear of Electrical Panel (E-25R) FUSE HOLDERS (see Figure 7d for details) New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 32 Figure 7c: Fuse Holder Detail (E-25) FUSE Figure 7d: Fuse Holder Detail (E-25R) FUSES 5. Remove the fuse and check it. If it has failed, replace the fuse. 6. Swing the to the left, flush against the unit. ELECTRICAL PANEL 7.

  • Page 33: Belt Replacement

    12. Move the motor mounting bracket back, until there is a slight resistance. 13. Verify that the drive belt has a slight pressure near the center. The recommended deflection is 3/8 inch (9.5 mm). New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 34 Figure 8: Drive Belt Replacement E-RING UPPER BEARING HOUSING FLEXURE NUTS FLEXURE ARM FLEXURE ARM NUTS COUNTERWEIGHTED PULLEY MOTOR PULLEY DRIVE BELT MOTOR MOUNTING BRACKET 14. Use the hex wrench to tighten the four hex nuts on the motor mounting bracket. 15.

  • Page 35: Troubleshooting

    Shaker is overloaded and/or you are using baffled flasks: remove some contents & balance load. Shaker does not run at set speed. Defective motor: call for service. Drive belt out of alignment or worn: call for service..continued... New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 36 Symptom(s) Probable Cause(s) & Solution(s) Load out of balance: unload all contents, then reload. Operating noise Loose component(s) in platform, subplatform and/or drive assembly: call for service. Heater fuse blown: replace. Compressor fuse blown: replace. Compressor over-pressure switch activated: call for service. Incubator does not Ambient temperature too high or too low: cool or heat the room reach set temperature.

  • Page 37: Replacement Parts & Accessories

    250 ml Erlenmeyer Flasks M1191-9910 500 ml Erlenmeyer Flasks M1191-9911 1 L Erlenmeyer Flasks AG-1 2 L Erlenmeyer Flasks AG-2 2.8 L Fernbach Flasks AG-28 4 L Erlenmeyer Flasks AG-4 6 L Erlenmeyer Flasks AG-6 New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 38 Table 4: Universal Platform Flask Capacity Flask Type Capacity 50 ml Erlenmeyer Flasks 125 ml Erlenmeyer Flasks 250 ml Erlenmeyer Flasks 500 ml Erlenmeyer Flasks 1 L Erlenmeyer Flasks 2 L Erlenmeyer Flasks 2.8 L Fernbach Flasks 4 L Erlenmeyer Flasks 6 L Erlenmeyer Flasks Table 5: Carriers &...

  • Page 39: Drawings

    HEATER 100Vac OPERATION I-24; I-25; I-26 TB002 RS232 10 Cond. Ribbon Cable DISPLAY BOARD Port BLACK M1299-8020 M1324-7002 WHITE YELLOW (REFRIGERATION OPTION) KEY PAD COMPRESSOR UNIT TB001 SEE DRAWING #2 FOR DIFFERENT VOLT OPERATION New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 40 Figure 9a: Control Schematic, Section A CB001 CB001 15 AMP 15 AMP SSR BOARD M1324-8011 M1353-8006 M1353-8006 M1324-7004 (REFRIGERATION OPTION) (M1324-7003) AC POWER ENTRY 100-240 VAC 50/60Hz Filter M1324-8013 line load HEATER 230Vac OPERATION TB002 BLACK POWER DISTRIBUTION WHITE DISPLAY DRIVER BOARD M1324-7001 YELLOW Figure 9b: Control Schematic, Section B...
  • Page 41 SEE DRAWING #2 FOR DIFFERENT VOLT OPERATION Figure 9d: Control Schematic, Section D CONTROL BOARD M1324-7000 Temp. Sensor,RTD M1195-8001 DOOR SW. E-24; E-25 I-24; I-25; I-26 RS232 10 Cond. Ribbon Cable Port M1299-8020 New Brunswick Scientific Co., Inc. User’s Guide...
  • Page 42 Figure 10a: 230V ac, 50 Hz Power Schematic 230Vac/50Hz OPERATION POWER TRANSFORMER M1324-8015 FROM TB001 M1324-7003 COND. FAN J4-1 ORANGE J4-3 M1324-8017 BLACK J4-5 WHITE J4-2 YELLOW M1324-8016 J4-4 VIOLET OVER PRESSURE J4-6 SWITCH COMPRESSOR UNIT M1324-8017 Figure 10b: 100V ac, 60 Hz Power Schematic 100Vac/60Hz OPERATION POWER TRANSFORMER...
  • Page 43 Figure 10d: 100V ac, 50 Hz Power Schematic 100Vac/50Hz OPERATION POWER TRANSFORMER M1324-8015 FROM TB001 M1324-7003 ORANGE COND. FAN J4-3 J4-1 M1324-8017 BLACK J4-5 WHITE J4-2 YELLOW M1324-8016 J4-4 VIOLET OVER PRESSURE J4-6 SWITCH COMPRESSOR UNIT M1324-8017 New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 44: List Of Drawings

    Figure 11: E-25R Refrigeration Schematic 10.1 List of Drawings Figure Description Page Space Requirements Front Panel 17, 25 Keypad Platform Screw Locations Double Girdle Clamp Installation Clamp Fastener E-25 and E-25R Rear Panel Top Right Bezel Removed Rear of Electrical Panel (E-25) Rear of Electrical Panel (E-25R) Fuse Holder Detail (E-25) Fuse Holder Detail (E-25R)

  • Page 45: List Of Tables

    10.2 List of Tables Table Description Page Clamp Hardware Application Chart Service Parts Platforms Universal Platform Flask Capacity Carriers & Test Tube Racks List of Drawings List of Tables New Brunswick Scientific Co., Inc. User’s Guide...

  • Page 46: Index

    Accessories · 37 Incomplete Order · 14 Actual Temperature · 28 Indicated Temperature · 28 Alarm Functions · 27 Inspection · 13 Ambient Temperature · 9 Installation Audible Alarm Platform · 21 Disabling · 27 Installing Flask Clamps · 22 International Offices ·...
  • Page 47 Temperature Correction Value · 28 WARNING · 5 Temperature Offset Calibration · 28 Warranty · 6 Temperature Setpoint · 27, 29 Warranty Registration · 14 Test Tube Racks · 38 Timed Functions · 26 New Brunswick Scientific Co., Inc. User’s Guide...

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