FEI Tecnai F30 Polara Operation

Operation o f t he F EI T ecnai F 30 P olara
Initial C hecks
1. Ensure t he f ollowing s oftware a pplications a re a ll r unning a nd o pen i n
this o rder:
-­‐ Tecnai U ser I nterface
-­‐ Filter c ontrol
-­‐ Digital M icrograph
-­‐ TEM I maging a nd A nalysis ( TIA)
If y ou n eed t o r estart t he s oftware c lose a ll p rograms a nd r estart i n t he
above o rder. S ometimes l ogging o ut a nd b ack i n a gain c an s ort o ut b ugs.
The u sername i s s upervisor a nd t he p assword i s f 30d316
2. Check t he v acuum. T he G un s hould b e a t L og 1 a nd t he C olumn a t L og 6 .
The C amera s hould b e b elow L og 3 5.
3. Make s ure t he H igh T ension i s O N ( High T ension b utton Y ellow) a nd t he
FEG i s O N ( Operate b utton i s Y ellow).
4. Check t he L N2 l evels i n t he i nner a nd o uter d ewars. T he i nner d ewar ( He
dewar) s hould b e b etween 1 3-­‐14 ( left d isplay) a nd t he o uter d ewar
should b e a bove 7 0%. T he i nner d ewar n eeds t opping u p e very 2 -­‐3 d ays.
The o uter d ewar w ill e mpty a t a r ate o f ~ 5%/hour a nd s hould n ot g o
below 2 0%.
5. Use t he m etal f unnel t o t op u p t he I nsertion R od d ewar. T his d ewar c an
last u p t o 1 0 h ours. I f t he I nsertion R od w arms u p t he v acuum
deteriorates a nd t he H T m ay s witch o ff. W hen c ollecting w ith E PU a L N2
top-­‐up a t 1 1pm w ill e nsure i t s tays c old u ntil 9 am t he n ext d ay.
6. Check t he t emperatures o f t he d ifferent c hannels o n t he d isplay:
1. H elium c ryo s hield
2. N itrogen c ryo s hield
3. H elium c old f inger
4. N itrogen c old f inger
5. G uide t ube
6. I nsertion r od
7. S election r od
7. If t he s pecimen S election R od i s s till a ttached t o t he m icroscope, m ove i t
to t he t ransfer s tation
-­‐ M ake s ure c olumn v alves a re c losed.
-­‐ V ent a irlock
-­‐ U nder S etup -­‐ > H elium -­‐ > V ent a irlock
-­‐ W ait f or m essage t o c lose v alve B
-­‐ C lose v alve B ( Pull o ut a b it, t hen p ush a ll t he w ay i n t o l ock)
-­‐ W ait f or m essage t o c lose v alve A
-­‐ C lose v alve A ( Pull o ut a b it, t hen p ush a ll t he w ay i n t o l ock)
-­‐ D etach t he t emperature l ead v ia t he U SB c onnection
-­‐ W ith o ne h and o n t he p lastic m anipulator a t t he b ase, r elease
clamps, r otate a nd l ift o ff h inges, m ove o ver t o t he t ransfer s tation
-­‐ P lace o n h inges, r otate f lush, f asten c lamps
(22/03/2016)