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Summary of Contents for Astrea Evolve
- Page 1 Evolve ® 350mm Column Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit...
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Page 2: Table Of Contents
Step 1 – Assemble Tube Unit onto the Column base and Bottom flowcell........16 Preparing for Column Use ....................18 Step 1 - Hydrostatic Leak Test ..................18 Step 2 - Levelling the 350mm Columns ................18 ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... -
Page 3: Replacing The Wetted Flowpath
Replacing the Wetted Flowpath This manual describes the procedures necessary to replace the wetted flowpath components in the Evolve ® 350 column using the Column Refresh Kit AA35CRK3. Description Flow Tube Adaptor 10mm Bore Adjuster Flow Tube with seals 10mm Bore... -
Page 4: General Guidelines
There are four main stages to refreshing the column by replacing the wetted flowpath. The first three stages are based on the disassembly and reassembling each column sub assembly with new components before the final stage of rebuilding and testing the complete column. STAGE 1 – Refresh the Adjuster Assembly of the Evolve ® 350 Process Column STAGE 2 –... - Page 5 Alignment indicators are attached to the top flange, tube flanges, bottom flowcell and column base. These assist with reassembly of the column and ensure correct orientation of the column components. Alignment Indicators Figure 2 Photograph of Evolve ® 350 showing alignment indicators.
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Page 6: Stage 1 - Refresh The Adjuster Assembly
Place the adjuster assembly so that the flowcell face is on the floor with the hoist still attached. Identify the parts as shown in the schematic and photograph below. Figure 4 Adjuster Assembly Evolve ® 350 column Figure 3 Photograph of Evolve ®... - Page 7 Height adjust handle assembly locking nut and gasket seal Adjuster seal Adjuster Flange Bed support retaining ring Seal setting ring Bed support retaining bolt Backing Plate ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit...
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Page 8: Step 2-Reassembly And Refresh The Adjuster Assembly
Using appropriate lifting slings attach the hoist to the adjuster assembly and lift the unit high enough to allow the flowcell assembly to be fitted. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... - Page 9 Do not over tighten as this may lead to failure of the component The refresh of the adjuster assembly is now complete. Ensure the unit is firmly seated on the floor and detach the hoist. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit...
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Page 10: Stage 2 - Refresh The Tube Unit Assembly
Lift up the column tube unit and when suspended loosen the tie bar fixing bolts that are located in the bottom column flange. Place the tube unit on a flat surface. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... - Page 11 Remove tie bars to allow the tube to be lifted out of the bottom tube flange. If tube is difficult to remove from flange remove the lower buffer seal. Discard the tube and buffer seals. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit...
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Page 12: Step 2-Reassembly And Refresh The Tube Unit
Check the tube support rod bolts in the bottom and top flange and tighten if necessary. The torque wrench and a setting of 50Nm is recommended. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... -
Page 13: Stage 3 - Refresh The Fixed End Assembly
Step 1-Disassembly of the Fixed End Assembly. To assist it is recommended that column feet are lowered to provide a stable base. Identify the components on the schematic shown in Figure 13. Figure 13 Fixed Assembly Evolve ® 350 column Using the correct size allen key, remove and retain the pipespool clamp top. -
Page 14: Step 2-Reassembly And Refresh Of The Fixed Assembly
Connect the pipespool elbow to the flowcell and secure in place by refitting the pipespool clamp top. Figure 14 Reassembly of Column Bottom Flowcell and Fixed end ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... -
Page 15: Stage 4 - Column Reassembly And Preparation For Use
The column is supplied with alignment indicators , these are attached to the top flange, tube flanges, bottom flange and base plate. These assist with the reassembly of the column and ensure correct orientation of components. Alignment Indicators Figure 15 Alignment Indicators on Evolve® 350 column ® Evolve... -
Page 16: Step 1 - Assemble Tube Unit Onto The Column Base And Bottom Flowcell
Remove the eye bolts from the top flange and store with other column tools. Attach appropriate lifting slings to the eye bolts located on the adjuster assembly. Lift the adjuster assembly. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... - Page 17 Locate the top flange fixing bolts/washers, initially, hand tighten, working diametrically opposite to ensure equal loading. Then using the correct spanner working diametrically opposite to tighten fully. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit...
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Page 18: Preparing For Column Use
Locate the flat portion on the column feet bolt and using the correct spanner adjust so that the bubble is located centrally on the spirit level. Turn the spirit level by 90 degrees and repeat. ® Evolve 350mm Column: Instructions for Replacing the Wetted Flowpath using the Column Refresh Kit... - Page 19 Evolve is a registered trademark of Delta Precision Ltd. Evolve is a registered trademark in the EU, UK and US. All other trademarks, trade names, trade dress, product names and logos appearing on this document are the property of Astrea Bioseparations Ltd.
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