Appendix D E-Gel ™ Clonewell ™ Ii Agarose Gels; General Guidelines; Prepare Samples - Invitrogen E-Gel Power Snap Plus User Manual

D
E‑Gel CloneWell
™
Electrophoresis Device to provide a fast, safe, and effective DNA fragment isolation method for DNA
cloning workflows.

General guidelines

• Load gel within 15 minutes of opening the pouch; run the gel immediately after loading.
• Monitor the band of interest carefully as it migrates near the recovery wells. It may be difficult to
see low amounts of DNA in the well.
•
IMPORTANT! Always wear Safe Imager
opened.
• For guidance on disposal of used gels, see SYBR
stain" on page 51).

Prepare samples

• Prepare up to 25 µL of sample in 1X Sample Loading Buffer (e.g., use 2.5 µL of 10X Sample
Loading Buffer with 22.5 µL total sample).
10X Sample Loading Buffer is provided with E‑Gel
• Use the indicated amount of DNA per well for single or multiple bands.
• Divide samples with higher amounts of DNA across multiple wells.
• Use up to 25 µL total sample volume per well.
• Dilute high salt samples (certain restriction enzyme and PCR buffers) 2- to 5‑fold.
Gel type
E‑Gel CloneWell
™
E‑Gel Power Snap Plus Electrophoresis System User Guide
™
E‑Gel CloneWell
™
II pre-cast agarose gels can be used with the E‑Gel
™
Sample with single band
II 200-800 ng
™
™
Viewing Glasses when viewing the gel with the filter lid
™
Safe DNA Gel Stain ("SYBR
™
CloneWell
™
Amount of DNA per well
Sample with multiple
bands
800 ng
II Agarose Gels
Power Snap Plus
™
Safe DNA gel
™
II Agarose Gels.
™
Total loading volume
25 µL
55