CONTENTS Chapter 1 - Muscle Strip Myograph overview ..................................3 Chapter 2 - setting up muscle strip myograph ..................................4 2.1 Changing and adjusting the mounting supports ...............................4 2.1.1 Changing the mounting supports ....................................4 2.2 Calibration of the force transducer ....................................5 3.1 Mounting supports and how to mount muscle strips ..............................6 3.2 Normalization ............................................7 3.3 Standard start ............................................7 3.5 In vitro experiment 1: Noradrenaline contractile response .............................7...
CHAPTER 1 - MUSCLE STRIP MYOGRAPH OVERVIEW Transducer house Micropositioner Connection to Interface Muscle Strip Myograph clamp support connected to micrometer Force transducer pin Muscle Strip Myograph clamp support connected to force transducer Figure 1.1 Muscle Strip Myograph with close-up of chamber CHAPTER 1...
CHAPTER 2 - SETTING UP MUSCLE STRIP MYOGRAPH 2.1 Changing and adjusting the mounting supports 2.1.1 Changing the mounting supports 2.1.2 Coarse adjusting the jaws for small vessels (figure 2.1) 2.1.3 Fine adjusting the jaws for small vessels (figure 2.2 and figure 2.3) 2.1.4 Fine adjusting the pins for larger vessels (figure 2.4 and figure 2.5): MUSCLE STRIP MYOGRAPH SYSTEM - 820MS - USER MANUAL...
2.2 Calibration of the force transducer As a part of the general maintenance of the Muscle Strip Myograph, DMT recommends that the Muscle Strip Myograph is force calibrated at least once a month. The Muscle Strip Myograph should also be force calibrated every time it has been moved. Al- though lab benches are all supposedly perfectly horizontal, small differences in lab bench pitch can affect the calibration of the system.
CHAPTER 3 - EXPERIMENTAL SET-UP 3.1 Mounting supports and how to mount muscle strips Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the Muscle Strip Myograph chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction. They are also designed to effectively “grab”...
CHAPTER 3 - CLEANING AND MAINTENANCE 3.1 Cleaning the Muscle Strip Myograph DMT STRONGLY RECOMMENDS THAT THE MUSCLE STRIP MYOGRAPH AND SURROUNDING AREAS ARE CLEANED AFTER EACH EXPERIMENT. At the end of each experiment, use the following procedure to clean the chambers and supports: 1.
IMPORTANT DMT RECOMMENDS USE OF HIGH VACUUM GREASE ONCE A WEEK TO SEAL THE TRANSDUCER HOLE BY FRE- QUENTLY USE. DMT TAKES NO RESPONSIBILITIES FOR THE USE OF ANY OTHER KINDS OF HIGH VACUUM GREASE THAN THE ONE PURCHASED FROM DMT.
3.2.2 Force Transducer Replacement If the force transducer breaks and needs to be replaced, follow this step-by-step replacement procedure carefully: 1. Remove the clamp from the transducer pin coming out of the transducer house. 2. Disconnect the Muscle Strip Myograph from the Multi Interface. 3.
3.3 Maintenance of the micropositioner Check the micropositioner for grease at least ONCE A WEEK. There are 3 main parts that will need to be greased on the mi- cropositioner, those parts being the micropositioner screw thread and the 2 slide bars to the left and right of the micropositioner screw thread.
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