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Related Manuals for Izon EXOID
Summary of Contents for Izon EXOID
- Page 1 EXOID USER MANUAL www.izon.com...
- Page 2 (TRPS) into a semi-automated machine with improved sensitivity and usability, the Exoid represents a reliable method of measuring individual particles. At Izon Science, we are committed to helping you get an accurate...
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Page 3: Table Of Contents
Important Information ........................11 ASSEMBLY AND SETUP INSTRUCTIONS ............12 Instrument power and general operation ................12 General Operating Procedures ....................12 Assembling and Installing the Exoid ..................13 Connecting to a computer ......................13 OPERATING INSTRUCTIONS ................15 Initial Connection ..........................15 Investigation Details ........................ -
Page 4: Definitions And Writing Conventions
This symbol indicates general advice on how to improve procedures or recommends measures to take in specific situations. This symbol indicates where special care should be taken. Direct Current (DC) 24V DC/4.17A Voltage polarity of the jack Crushing Hazard www.izon.com... - Page 5 Calibration Aliquot of diluted calibration particles of a known size. Exoid buttons The buttons near the top of the Exoid, to control the stretch. Electrolyte A liquid which contains ions and can transmit a current when voltage is applied.
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Page 6: Safety And Hazards
4.17 A max current 100.08 W max power Hazards The Exoid is a laboratory product, however if any biohazardous samples are present then adhere to current good lab practices (cGLP) and comply with any local guidelines specific to your laboratory and location. - Page 7 Fire or Electrical Hazard The Exoid poses no uncommon electrical or fire hazard to operators if installed and operated properly without physical modification and connected to a power source of correct specification. Chemical Hazards The Exoid system contains no potentially hazardous chemical materials.
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Page 8: Introduction To The Exoid
Ag/AgCl Electrode Electrolyte Particle flow Upper fluid cell Nanopore Electrolyte Lower fluid cell Ag/AgCl Electrode Figure 1. A schematic representation of how TRPS works within the Izon system. www.izon.com... - Page 9 The APS is also capable of applying pressures (positive and negative) 50% greater than that of the VPM. Visual indications of the state of the Exoid using the Exoid lighting allow the user to immediately assess how the system is functioning.
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Page 10: Fluid Cell V2
Fluid Cell V2 The Exoid system comes with a new and improved fluid cell design. A dedicated return path built into the electronics of the fluid cell and improved shielding mean that the entire system is more resilient to external noise interference. This new design is also less susceptible to the “short circuits”... -
Page 11: Important Information
Important Information Pipetting While using the Exoid it is important to employ correct pipetting practices. For all particle and sample dilutions the user should use forward pipetting, reverse pipetting should be used when pipetting into the upper fluid cell. For more information on these two pipetting techniques visit support.izon.com/pipetting-techniques... -
Page 12: Assembly And Setup Instructions
Position the unit so it can be quickly and easily disconnected from the mains power. Check the local supply meets the AC input requirement given in the specification. Izon instruments are only to be operated with Izon supplied leads and power supplies. Failure to use the correct power supply may result in invalid operation. -
Page 13: Assembling And Installing The Exoid
Connect the power lead to the 24 V power supply. Make sure the power is off at the wall and at the Exoid before plugging the power lead into a wall socket and connecting the cable from the 24 V power supply to the rear of the instrument. - Page 14 To operate the Exoid, there are two software suites that must be installed. The Exoid Control Suite (ECS) is used to control the Exoid and gather data. The Izon Data Suite (IDS) is a separate software required to analyse the data collected in the ECS. Both software packages for non 21 CFR Part 11 installations can be downloaded from the link below.
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Page 15: Operating Instructions
OPERATING INSTRUCTIONS Initial Connection If using a laptop to operate the Exoid, ensure that the laptop charging cable is plugged in and providing power at all times during operation of the instrument. Once the instrument is connected to the computer according to the earlier instructions, open the ECS. -
Page 16: Investigation Details
The Exoid will do this automatically once the user has followed the setup prompts, if there are any issues during this process then the ECS will notify the user that intervention is required. - Page 17 Izon coating solution powder is available for purchase with the Izon Reagent Kit, which will also provide all other reagents needed for nanoparticle analysis on the Exoid.
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Page 18: Sample Analysis
The ECS will automatically stop recordings at 500 particle count before moving on to the next pressure step. The ECS will guide the user through analysing the sample, the first step being to run a calibration for the system. www.izon.com... -
Page 19: Guide To Real Time Data
Zooming out will give a much better visual for the particle blockades, however zooming out too far will make it extremely difficult to detect an increase in RMS noise quickly. www.izon.com... - Page 20 Particle size distribution histogram This graph is located to the right of the particle size plot when the trace is popped out, it will display the size distribution of the unknown particle being analysed on the Exoid in real time. www.izon.com...
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Page 21: Guide To Real Time System Metrics
If the user does not elect to continue with further samples then they will be guided through a nanopore cleaning process. The ECS will guide the user through removing the nanopore and cleaning the necessary hardware appropriately. Instructions can be found at https://support.izon.com/what-is-the-best-practice-for-cleaning-the- nanopore www.izon.com... -
Page 22: Types Of Analysis
Size and zeta measurements For zeta measurements there are specific electrolytes which must be used, outlined in the ECS, please contact Izon Support if this requirement is an issue for the sample or there are problems obtaining suitable materials. Not all nanopores are suitable for zeta analysis, to help with this the user can specifically request zeta capable nanopores by contacting Izon Support. - Page 23 Signal trace with resistive pulses Figure 2. A correlation of real time data visualised during data collection paired with post processing blockade data. www.izon.com...
- Page 24 A particle with a larger negative surface charge will experience a greater attraction towards the positively charged electrode in the lower fluid cell and will travel through the nanopore at a greater speed than a more neutral particle. www.izon.com...
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Page 25: Manually Controlling System Parameters
100-140 nA. If the stretch has been decreased, the voltage will need to be increased and vice versa. STRETCH (MM) 43.5 49.5 Optimal operating stretch Very stable recordings, but nanopore is at risk of being stretched out of shape www.izon.com... -
Page 26: Applied Pressure
On some occasions, excessive stretch can cause a rupture of the nanopore. The Exoid has built in limit switches to prevent the stretcher being moved to a position that will damage the winding mechanism, these are located approximately at 41 and 51 mm. - Page 27 It is proportional to particle surface charge (ζ-potential) and the applied voltage Electro-osmosis - Electro-osmosis relates to the fluid flow caused by currents of solvated ions moving along the surface of the nanopore. It is proportional to the nanopore surface charge (ζ-potential) and the applied voltage www.izon.com...
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Page 28: Troubleshooting
There may be many invisible bubbles beneath the nanopore. Put a droplet of electrolyte on top of the nanopore, take the nanopore off the Exoid and wipe the bottom gently with a lint free tissue before remounting on the Exoid and applying the same stretch as before. Re- establish your baseline current. - Page 29 Stable baseline current, but — Nanopore is partially blocked by a bubble or an aggregate. very few particles Un-block nanopores by using the Izon Pressure Application Device (PAD). — Mix sample with pipettes or by gently inverting/swirling samples by hand, vortexing or sonicating can damage biological nanoparticles.
- Page 30 — If the sample particles have positively charged surface/ terminal groups, they are likely to stick to and block the nanopore instead of going through. Contact Izon Support if this may be the case. Short-circuits Electrolyte can creep in between metal connection points inside or around the fluid cell, causing significant noise fluctuations.
- Page 31 Follow these instructions carefully when the need to power cycle the instrument arises. Turn off the switch on the Exoid, the off position is where the full ”O” symbol is pressed down as indicated in the image below. Unplug the USB and power cables from the Exoid.
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Page 32: Further Support
DO NOT soak any part of the fluid cell. Repair and Servicing There are no user-serviceable parts. Return equipment to Izon for service. All replacement parts, cables, mains lead, power supply, Nanopores, Fluid Cells must be obtained via Izon Science Ltd or from an authorised distributor. - Page 33 This document is not part of any sales contract between Izon Science Ltd. and a purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of Sale shall govern all conflicting information between the two documents.
- Page 34 FIND OUT MORE Visit www.izon.com for more information...
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