UVITEC Cambridge UVIDOC HD6 Quick Start User Manual
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UVITEC Cambridge UVIDOC HD6 Quick Start User Manual

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  • Page 1 UVIDOC © U I C K T A R T S E R U I D E...
  • Page 2 On behalf of Uvitec Ltd, we would like to thank you for choosing the UVIDOC HD6 imaging system. In order to learn the capabilities of your UVIDOC HD6 imaging system, we kindly ask you to read this manual. This manual details how to install and to operate the hardware and the software components.

  • Page 3: Table Of Contents

    Imager front side – Door open ............................22 Filter wheel ................................... 22 System operation .................................. 23 Introduction ..................................23 Start the UVIDOC HD6 system & software ........................24 Capture an image ................................25 Fluorescence image acquisition ............................ 25 Image capture workflow ............................... 25 Choose the application type ............................
  • Page 4 Connect to the Uvidoc from your computer ........................53 Connect using your internet browser ..........................53 Connect using the Uvitec1D software ..........................53 Update the Uvidoc HD6 firmware: ........................... 56 Technical information ............................... 57 Electrical specifications ..............................57 Climatic conditions ................................ 57 Spare Parts ..................................

  • Page 5: Trademarks

    Notice No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopy, recording, or any information storage or retrieval system, without permission in writing from Uvitec Ltd. Uvitec Ltd reserves the right to modify its products and services at any time without prior notice. The contents of this user guide are subject to change without notice.

  • Page 6: Introduction

    Introduction Prerequisites The User Guide provides you with the instructions needed to operate, and maintain the UVIDOC HD6 imaging system in a safe way. In order to operate the UVIDOC HD6 imaging system in the appropriate way, the following prerequisites must be fulfilled: •...

  • Page 7: Warranty

    Warranty The UVIDOC imaging system is warranted against faulty construction or defective material for a period of two years from the Uvitec Ltd invoicing date. If any defect occurs in the instrument during this warranty period, Uvitec Ltd will repair or replace the defective parts at its discretion without charge.

  • Page 8: Safety Instruction

    Safety instruction Introduction The UVIDOC imaging system is powered by mains voltage, may be equipped with a UV light source, and is used to capture images of samples that may be hazardous. Before installing, operating or maintaining the instrument, you must be aware of the hazards described in the user documentation.

  • Page 9: General Precaution

    General precaution WARNING Do not operate the UVIDOC imaging system in any other way than described in the User Manual. WARNING Use of this instrument in other ways than those specified in the user documentation may result in physical damage because of exposure to irradiated light or electric shock or injury from touching an operating part.
  • Page 10 WARNING Ensure that all the ventilation-opening systems are not obstructed. The obstruction of the air admission grids may affect the performance of the system and cause operational failure. To ensure adequate cooling ensure there is at least 40 cm of free space in front of the instrument and at least 10 cm on all other sides to walls or other equipment.

  • Page 11: Important Uv Safety Information

    WARNING • Do not place objects on top of the instrument. • Use the handle to open or close the instrument door. • Handle sample trays with care to avoid dropping them. • Do not leave samples in the instrument after exposure. If left, these may degrade and cause damage to the instrument.
  • Page 12 UV WARNING Do not touch the UV unit after UV exposure. There is a risk of skin burn. UV WARNING The instrument is equipped with a safety interlock. If the interlock is out of order or has been tampered with, UV and visible light may be emitted, which may cause skin burn and impair vision. Do not open the instrument door during imaging operation.

  • Page 13: Important Electrical Power Safety Information

    WARNUNG: während der Benutzung dieses Gerätes kann die Missachtung der Anleitung schwere Verbrennungen an Personen hervorrufen. ACHTUNG: Ultraviolettes Licht ist gefährlich für ungeschützte Augen und die Haut. Der Benutzer ist daher angehalten, eine UV-Schutzbrille (Art. LP-70) oder ein Schutzschild (Art. MP-80 oder MP-800) zu tragen. BEWAHREN SIE DIESE ANLEITUNG SORGFÄLTIG AUF 警告：使用此组件时，若不遵从使用指导将导致严重烧伤！...
  • Page 14 WARNING Do not damage the power supply cord by bending, twisting, heating or allowing them to become pinned under the equipment. Using damaged power cords could result in fire or electric shock. If the power supply cords are damaged, contact us for replacements. Never pull the cable itself.
  • Page 15 WARNING Do not turn off power during image acquisition as this can cause loss of data and damage the instrument. Only turn off power in an emergency situation. WARNING - Electrical shock hazard. All instrument repairs or modifications should be performed by service personnel authorized by Uvitec Ltd.

  • Page 16: Uvidoc Hd6 System Installation

    UVITEC-1D software CD-Rom inside the instruction manual  Remove carefully each component from the box.  To remove the UVIDOC HD6 from the box, please use the belts which surround the system and designed for this purpose  Do not handle the system by the camera or the camera support.
  • Page 17 Do not connect the power cable to a power source until all connections are made. The power source has to be grounded and protected by a circuit breaker. WARNING The UVIDOC HD6 system is designed to fit a specific voltage. Please, check the voltage to ensure it corresponds to the UVIDOC HD6 specifications. WARNING Please keep an open area of at least 20 cm at the rear of the cabinet to ensure a proper air circulation for the system.

  • Page 18: Installing The Hardware

    (next to a centrifuge or to a compressor for instance) Remove all hot or heat source near the UVIDOC HD6, specifically from the air intake. Do not place objects near the power outlet to ensure easy access to the power cord for disconnection in case of emergency.

  • Page 19: Imager Back Side

    Imager back side Electrical requirements The system power supply and main switch are positioned at the back side of the instrument. Connect the imager power cord to the power supply plug (1) and plug into a grounded power outlet. A grounded circuit capable of delivering the appropriate current and voltage is required for installation.
  • Page 20 The USB port of the left side of the instrument (3) is designed to hold your USB key drive for image saving. The imager on/off switch (4) starts or stops the Uvidoc HD6 and activates the internal computer, touch screen and...

  • Page 21: Operating Instruction

    Operating instruction Warning while using the darkroom UV WARNING Switch off the transilluminator when gel is not present on the UV filter. If the filter is too hot, it will damage your electrophoresis gel. UV WARNING Wait for at least 20 second in the "High" position before reducing the intensity selector to "Low”. UV WARNING If one or several tubes are off or used, and in order to keep a better homogeneity, we recommend to change the 6 tubes simultaneously.

  • Page 22: Imager Front Side - Door Open

    The white light switch on automatically when the door is opened. Filter wheel The Uvidoc HD6 main switch is located inside the darkroom. The 3 positions filter wheel is equipped with the F590 M58 interferential filter supplied with the system for Ethidium bromide application.

  • Page 23: System Operation

    This chapter describes how to operate the UVIDOC HD6 imaging system. Before to operate the UVIDOC HD6 system, it is important that you have read and understood the safety information. Make sure to read this User Guide relevant safety instructions before you start to operate the system.

  • Page 24: Start The Uvidoc Hd6 System & Software

    On the left hand side of the imager, press the power button to the position I, to turn it on. After the computer has booted up completely, the following messages are displayed until the Uvidoc HD6 system is ready for image acquisition:...

  • Page 25: Capture An Image

    The Uvidoc HD6 system separates the emitted light from the excitation light in order to obtain an optimum sample image. According to the configuration, the Uvidoc HD6 can accommodate several excitation and emission channels in the visible, and UV area and is ideal for a large array of applications such as 1D DNA gel, 1D protein colorimetric samples, multiplexing, stain free gel and blot.

  • Page 26: Choose The Application Type

    Choose the application type The Application selector The Uvidoc HD6 runs with pre-defined image acquisition parameters to facilitate the use of the system for a specific application. These pre-defined parameters are gathered in the Uvidoc HD6 Application Protocol and can be selected...

  • Page 27: Acquire The Image

    For a colorimetric sample using epi white light (light from the top), select the White light application from the Application Selector window. Acquire the image The Uvidoc HD6 system has three exposure mode: • The live mode • The auto mode •...
  • Page 28 The selection of the Live mode will open the following menu: Enter the duration of the exposure. Define your exposure time using the plus and minus buttons: Modify the zoom and focus settings. The Zoom in allows you to change the size of your sample, on the image. To proceed, click on the Plus or Minus button. The zoom automatically moves to the new value.
  • Page 29 Click on Stop to finalize the image acquisition process. The Auto mode The Uvidoc HD6 system calculates automatically the optimum exposure time (Auto-exposure). When you select the Auto-Exposure option, the system samples the light levels and uses the values to calculate the final exposure time.
  • Page 30 Enter the duration of the exposure. Define your exposure time using the plus and minus buttons: Note: The integration time increase or decrease by 40 milli-seconds, except for 10 and 20ms. Note: With long integration time, a delay could be necessary before an image is displayed on the monitor (up to twice the selected Exposure time).
  • Page 31 Image dynamic: The image dynamic is crucial for quantitative accuracy. It should to be the largest possible within the gray scale of the system.  65 535 gray levels for 16-bit imaging The gray scale is the total number of levels between white and black. The image dynamic range within this scale. Adjustments are necessary to obtain an image with the broadest dynamic.

  • Page 32: Save Or Print The Image

    Save or print the image Clicking on Save will start the saving of the captured image. The image is saved on the USB® key drive, according to the default image format. The Uvidoc has an internal memory, interrelated with the USB external saving drive. When pressing the “Save” key, the image is saved at the same time both on the internal memory and on the USB key drive.
  • Page 33 The Display option menu is helpful to enhance the image display by modifying the image greyscale selection to be displayed. In standard, the Uvidoc HD6 images have 16-bit format and Windows® can only display 8-bit images (256 grey levels). Due to this limitation, the software handles two images: •...
  • Page 34 The software offers the possibility to select the grey level range to translate for the display image calculation. All the grey levels under the “Min value” defined will be converted to 0 (Black) in the displayed image. All the grey levels upper the “Max Value”...
  • Page 35 The image displays presets goes from the minimum image grey levels to 20%, 40%, 60% or 100% of the image depth. Case 1: Case 2: Default optimum display Optimum display enhancement The image appears brighter. The faint bands are more visible. Note: The Display function inform you of the minimum and of the maximum grey level obtained on the image.

  • Page 36: The Application Protocol

    The Application protocol What is an Application protocol? The software runs with pre-defined image acquisition parameters to facilitate the use of the system for a specific application. These pre-defined parameters are gathered in the software Application protocol. The Application protocol automates a task or a set of tasks that you perform repeatedly or on a regular basis. It stores the following information: - The image exposure pre-defined set-up;...

  • Page 37: How To Access The Application Protocol

    How to access the application protocol? Select an application protocol from the application menu. Click on the application name to open the application protocol menu. The Application protocol can either be displayed with a summary view or with a complete view. By default, the application protocol is displayed with the summary view.

  • Page 38: Advanced Exposure - Exposure Predefined Set-Up

    Advanced exposure – Exposure predefined set-up The Advanced Exposure folder contains the exposure pre-defined set-up: Typical Exposure set-up for a fluorescence application...
  • Page 39 • Zoom and big size options The Big size options allows you to use the Uvidoc in a landscape mode with a larger field of view. For the displays, the landscape is rotated by 90°. The standard mode allows you to use the Uvidoc in a portrait mode. Big size not activated: standard mode Big size activated: landscape mode You can select till 6 zoom preset positions.
  • Page 40 • Fast mode / Standard mode The fast mode provides lower image resolution to speed-up the imaging process. • Auto-exposure set-up Test time In order to calculate an ideal exposure time, the software needs to analyze a test image, taken in binning mode with a test exposure time.
  • Page 41 Over exposure (faint band) results in a longer exposure where faint bands are more visible, but more prominent bands may be over exposed. Under exposure (bright band) results in a shorter exposure where bright bands are more visible, but more prominent bands may be under exposed.

  • Page 42: Preview - Live Preview Pre-Defined Parameters

    Preview – Live preview pre-defined parameters Preview folder contains the live review pre-defined set-up: • Extended resolution The effective pixels technology allows the extension of the camera native image resolution by software interpolation. Note: This process requires important computer calculation and sometime could be necessary before the extended image is displayed.

  • Page 43: Display - Image Display Pre-Defined Parameters

    The lens prefixed position table summarizes the default focusing preset per zoom option for the concerned Application protocol, when modified by the user. Display – Image display pre-defined parameters The Display folder contains the image display pre-defined set-up:...
  • Page 44 • Select display mode The Select display mode options allow you to select between a standard display, an enhance display for the faint band and an enhance display for the bright band. Standard display is optimized for all type of image. Enhance display for faint band ensure the faint bands are more visible, but more prominent bands will look very strong.
  • Page 45 A gamma curve is smooth: there are no unexpected jumps or cut-offs. This means that when viewing a gamma adjusted image, you will be able to see the details (intensity differences) in both the black and white areas of the image. When the bright areas of these types of images are correctly exposed, the darker areas can be so dark that they are in effect invisible.

  • Page 46: Printing - Image Printing Pre-Defined Parameters

    Printing – Image printing pre-defined parameters The Printing folder contains the image printing pre-defined set-up: • Print with image name Print the image with its name. • Print with date and time Print the image with the computer time. • Print out text color Select the color of the text printing.

  • Page 47: General - Image File Pre-Defined Parameters

    General – Image file pre-defined parameters The General folder contains the image file pre-defined set-up •User name You can define the name of a user for an application protocol. The user could be the name of a working group, the name of a user or a subtitle to the name of the application.
  • Page 48 •File format Images can be saved in CompatibilityPlus TIFF scientific image format (16-bit format recommended), TIFF scientific image format (16-bit format), BMP (8-bit format only), JPEG (compressed) or APX (proprietary file format – 16-bit format). This option allows you to select the default file format. •Preferred image saving directory This option defines the directory where the image is saved by default Alternatively, you can select to always open the last used directory by clicking on the following option:...

  • Page 49: The System Settings

    The System settings Settings feature allows the adjustment of the system settings such as the filter controls or the lens calibration. Click on “Settings” from the menu : A window displays the following menu:...

  • Page 50: The General System Settings

    The Settings menu contains the following features, related to the Uvidoc system: General Displays the Uvidoc information such as the system option, the software version, the filters installed and the lens calibration state. Langage selection Select the language you want the software to run Mail to Input the mail receiver address Windows parameters...

  • Page 51: Windows Parameters

    Lock / unlock the Uvidoc system The Uvidoc HD6 system is write-protected to prevents the installation of any none approved programs or files such as malware or virus. Thanks to the write-protected function, all files that are not write-protected are removed with a restart of the system.
  • Page 52 The Uvidoc can be connected to your computer through the local area network (LAN). A LAN is a communication network connecting a group of computers, printers, and other devices located within a relatively limited area (for example a laboratory). A LAN allows any connected device to interact with any other on the local network. For instance, any computer connected on the LAN could download an image.

  • Page 53: Connect To The Uvidoc From Your Computer

    You access the Connect to Uvidoc function thanks to the Exposure menu. Then, click on the “Connect to Uvidoc” button to access the function options. To do so, you need first to set an IP address and a subnet address for your Uvidoc HD6 system. Connection parameters Uvitec 1D provides connectivity between your computer and the Uvidoc through the local area network (LAN) on which is connected your Uvidoc system.
  • Page 54 By default, we recommend to select DNS (automatic IP address assignment from your network): If this option is not working properly, unselect the DNS option: Fill-in the Uvidoc IP address with the IP address defined in the Uvidoc HD6 system. Then, validate by clicking on OK.
  • Page 55 Uvidoc need password This option is not used with the Uvidoc HD6. The older Uvidoc system required a password for the ftp access. If you are using an old version of the Uvidoc system, please select Uvidoc need password for your ftp access. The password is then Uvidoc .

  • Page 56: Update The Uvidoc Hd6 Firmware

    To Install, please Unzip the Uvitec files in order to have, in the root directory of your USB stick, the following files: * Setup.exe * Setup.zip Plug the USB stick on the UVIDOC HD6 Then, follow the Uvidoc system update procedure using the settings function: Configuration/settings/ windows parameter/ Software update Select the USB stick directory and the Setup.exe file, then click on OK.

  • Page 57: Technical Information

    Technical information Electrical specifications Power supply ▪ Current (A) = 1A/0.5A ▪ Fuse FST (A) = 2A ▪ Voltage (V) = 100/230V~ (5%) ▪ Frequency (Hz) = 50/60Hz ▪ Power = 85 watts Fuses ▪ Type FST. ▪ Time-lag T. ▪...

  • Page 58: Spare Parts

    Spare Parts General advices • To clean the surface of the filter, use mild solvent or warm water. Dry with a soft cloth • The filter is porous, so try to keep it dry TYPE OF FUSE All our units are fitted with one or two safety fuses. Type FST They are found in the plug at the rear of the unit Time-lag T...

  • Page 59: Warranty

    Warranty ® Our products (except Compact Flash , light tubes and filters) are warranted against faulty construction or defective material for a period of TWO YEARS from the date of supply. Our products are not warranted for damage due to carelessness, incorrect use or bad maintenance.
  • Page 60 Manufacturer cannot be held responsible for any loss, bodily injury or material accident incurred by any failure of this supply, whatever the origin of this failure may be. The responsibility of Manufacturer is strictly limited to its staff and to its own supplies. In the case of dispute, only the commercial court of Cambridge (United Kingdom) shall be competent, even in third party claims proceedings or when there are several co-defendants.

  • Page 61: Conformity

    Conformity This system complies with the requirements of the EC Directive 2004/108/CEE, 2006/95/EEC and EN 61010-1, relating to Electro-magnetic compatibility and low voltage. The Electro-magnetic susceptibility has been chosen at a level that gains proper operation in residential areas, on business and light industrial premises and on small-scale enterprises, inside as well as outside of the buildings.

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