Load Method - Ankom Flex Analyte Extractor Operator's Manual

Operator's Manual

Load Method

From the Home Screen:
8.4.1. Press "METHODS."
8.4.2. Select the desired method category.
8.4.3. Select the method of choice. Refer to the ANKOM website for the
standard operating procedures of each method.
Analysis Options using the ANKOM
Method Name
VitAED 75C 45m
TotFat 3N HCl
CrudeFat
Cholesterol
NOTE:
The base methods can be adjusted slightly to change digestion parameters, conserve
solvent, etc. Please contact ANKOM for more details.
.
8.4.4. The screen will display the steps required to start a
run. Press the first step, "1. Enter sample name."
pg. 32
FLEX
Analyte Extractor
Method Description
This method includes ethanolic saponification at 75°C for 45min,
extraction through SPE (solvent: n-hexane), and evaporation of the
solvent at low temperature. The analyte can then be reconstituted for
chromatographic quantitation.
This method includes acid hydrolysis of the sample, filtration of the
aqueous portion to waste, extraction of the hydrolysate with solvent,
filtration with SPE, and evaporation of the solvent. The isolated fat can
then be gravimetrically quantified.
This method includes extraction of the sample with solvent, filtration
of the solvent extract through SPE, and evaporation of the solvent. The
isolated fat can then be gravimetrically quantified.
This method includes ethanolic saponification at 75°C for 45min,
extraction through SPE (solvent: n-hexane), and evaporation of the
solvent at low temperature. The analyte can then be reconstituted for
chromatographic quantitation.
Rev 1/22/2021