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MODEL 153
GRADIENT STATION
Manual version 3.3
SD Card Memory System
Fractionator software version 8.03
Gradient forming software version 5.3
OPERATOR'S MANUAL
© 2013 BioComp Instruments, Inc.
650 Churchill Row, Fredericton
New Brunswick, Canada E3B 1P6
Ph: (800)561-4221; (506)454-6410;
Fx: (506)455-6157; Email: dhc@biocompinstruments.com
Home Page: <http://www.biocompinstruments.com>
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Summary of Contents for Biocomp GRADIENT STATION 153
- Page 1 Manual version 3.3 SD Card Memory System Fractionator software version 8.03 Gradient forming software version 5.3 OPERATOR'S MANUAL © 2013 BioComp Instruments, Inc. 650 Churchill Row, Fredericton New Brunswick, Canada E3B 1P6 Ph: (800)561-4221; (506)454-6410; Fx: (506)455-6157; Email: dhc@biocompinstruments.com Home Page: <http://www.biocompinstruments.com>...
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Page 2: Table Of Contents
TABLE OF CONTENTS PART 1. SPECIFICATIONS AND SETUP. SECTION 1. INTRODUCTION 1.1. Warranty and service 1.2. Maintenance 1.3. Electrical surge protection 1.4. Goods damaged in transit 1.5. Instrument overview SECTION 2. SPECIFICATIONS 2.1. Physical measurements 2.2. Commercially available parts 2.3. Performance characteristics SECTION 3. - Page 3 PART 2. GRADIENT MASTER MANUAL PART 3. GRADIENT FRACTIONATOR MANUAL PART 4. GRADIENT PROFILER MANUAL (where applicable) PART 5. GRADIENT RUN PARAMETERS (last pages)
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Page 4: Section 1. Introduction
GRADIENT STATION, you may return it for a full refund. BioComp warrants this instrument to be free of defects in workmanship for a period of one year from the date of receipt for all electronic and mechanical parts. Liability is limited to repairs or replacement of the unit at BioComp's discretion. -
Page 5: Instrument Overview
BioComp absorbing unnecessary expenses. 1.5 INSTRUMENT OVERVIEW The Gradient Station combines the best of BioComp’s two premier gradient instruments in one. The Gradient Master is embodied by a tilting and rotating disk on the right wing of the Piston Gradient Fractionator. -
Page 6: Section 2. Specifications
SECTION 2. SPECIFICATIONS 2.1 Physical Measurements Shipping Weight: 52 lbs. Dimensions: 19.25”W x 16.5”D x 20”H (piston down), X 27"(piston up) Operating temperatures: Ambient (Cold room only if left turned on in the cold room and for 1 hr after return to room temperature to prevent condensation) Input Power: 90-240VAC, 0.4A, 50-60Hz. -
Page 7: Section 3. Parts Diagrams
SECTION 3. PARTS DIAGRAMS 3.1 THE GRADIENT STATION... -
Page 8: Gradient Forming Attachment
3.2 GRADIENT FORMING ATTACHMENT Tilt motor Rotate plate Bearing Rotate motor SECTION 4. ORDERING INFORMATION The Gradient Station™ gradient forming and fractionation system. 4.1 Base Unit: Cat No. 153-001 90-120VAC input; 153-002 220-240VAC input 4.2 Gradient Forming Accessories 2 cannula 1 bubble level 4.2.1 Tube Holders: (each tube holder is shipped with a marker block and 6 or 12 caps, I, R or IR) 105-925-I, R or IR: 25 mm SW28 tube holder... -
Page 9: Tube Holders
5.1 INSPECT THE SHIPPING BOX. If there is any damage to the outside that corresponds to damage to the instrument, CALL BIOCOMP IMMEDIATELY. To unpack the instrument, first open the top of the box and remove all the small parts and loose foam. -
Page 10: Assemble The Left Wing
SAVE THE SHIPPING BOX FOR POSSIBLE RETURN OF THE INSTRUMENT. 5.2 Assemble the Left Wing: There is a D-shaped black plate in the box. Use the flat head screws and the 1/8” hex wrench to assemble it to the top plate in the position shown. It extends the working area for tube racks. 5.3 Assemble the Gradient Forming attachment: Carefully remove the Gradient Forming attachment from its box and attach it to the right wing position. -
Page 11: Attach The Reservoir
5.4 Attach the Reservoir: There is a 60 cc syringe in the accessory kit. Pull the plunger out and connect it to the luer-loc connector at the end of the blue tubing on the right side of the box (pull the tubing out first). Slide the flanges on the top of the syringe barrel under the screw heads on either side of the notch in the right wing. -
Page 12: Assemble The Computer Panel
5.5 Assemble the computer panel. Find the green multi-wire plug and the 2 smaller, white 4-wire motor plugs inside the main unit and expose them as shown below. Note that the wires on the plugs are labelled “tilt” or “rotate”. Bring the front panel close to the instrument with its open end up. -
Page 13: Install The Power Cord
Find the Phillips screw driver and 2 of the 8-32 screws in the Accessory Pack. Attach the front panel to the main unit by inserting its leading edge under the bent edge of the front panel and resting the two flanges on the screw heads already installed on the side panels of the main unit as shown. - Page 14 Turn the power switch on. After a brief delay, the display will light up a pale green and a bit later the display will read: The instrument is preprogrammed and is now ready to use The row of 5 blank keys directly below the display are the “smart” keys whose values are determined by the words above;...
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Page 15: Section 6. Gradient Forming - Station
Section 6. GRADIENT FORMING - STATION 1.1 LEVELLING THE PLATE: A CRITICAL FIRST STEP 1. When you power up the device, the display reads: GRADIENT STATION v1.03 FRAC GMST Press GMST. After the Gradient Master software has loaded, the display will read: Reset KNOB to 0 position Position = 29 Turning the knob until the indicator line is up center (12 o’clock) changes the display to read:...
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