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Table of Contents
Related Manuals for Millipore Sigma Scepter 3.0
Summary of Contents for Millipore Sigma Scepter 3.0
- Page 1 User Guide Scepter™ 3.0 Handheld Automated Cell Counter PHCC30000 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada. The life science business of Merck operates SigmaAldrich.com as MilliporeSigma in the U.S. and Canada.
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Page 2: Table Of Contents
Contents Introduction . . . . . . . . . . . . . . . . . . . . 2 Symbols and Safety Precautions . -
Page 3: Symbols And Safety Precautions
Symbols and Safety Precautions This system is intended for research use. Go to SigmaAldrich.com/techservice for assistance. IT IS IMPORTANT TO READ THESE INSTRUCTIONS BEFORE USING THIS INSTRUMENT . • Please read these safety instructions and user manual carefully before using the cell counter and/or any accessory supplied with this product. -
Page 4: Parts And Functions
Parts and Functions Features Display Screen • Displays all information Tech Service Port needed for operation For Service use only • Displays histograms with with cell concentration, and diameter or volume USB Port Connects instrument to Eject Button USB thumb drive Ejects Sensor Ergonomic Hook •... -
Page 5: Battery
Battery The Scepter™ 3.0 is equipped with an internal 3.7 V lithium ion battery that recharges in the Charging Station. Install the Charging Station (see page 6) and plug into AC wall outlet. Place Scepter™ 3.0 onto the Charging Station. REGHARGING will be displayed. -
Page 6: Installing The Charging Station
Installing the Charging Station Figure 1: Wall area required for mounting Charging Station. Scepter™ 3.0 can be placed on the benchtop horizontally or 3.125 in. vertically wall-mounted, within 5 feet (1.5 meters) from an (82.5 mm) 1.56 in. electrical outlet. The mounting kit is required to mount the (39.6 mm) Charging Station. -
Page 7: Getting Started
Getting Started To turn on, make sure Scepter™ 3.0 is fully charged and press/hold the MENU button. Release when the vibrant M appears on screen. For setup, the sensor is not needed, press MENU to advance to Settings. Use the ARROW keys to scroll to Settings, press OK. It is recommended to set up all aspects of Scepter™... -
Page 8: Wi-Fi ® Network
Wi-Fi Network ® Materials required to link Scepter™ 3.0 to your Wi-Fi network: ® • Wireless Network broadcasting at 2.4 Ghz • USB thumb drive formatted to FAT32 (FAT32 is a common filesystem format compatible with Windows, Mac OS X, and Linux) ®... - Page 9 Creating a Wi-Fi Configuration File ® A “Wi-Fi configuration file” is needed for set up or to change Wi-Fi networks. ® ® On a computer, open up the text editing program. The examples below are using Microsoft Windows Notepad. ® ®...
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Page 10: Gating Method
Gating Method There are two options for setting the default gates (upper and lower diameter or volume limits of the histogram) prior to counting. One option is to choose “Use Last” and the other is “Auto” which automatically sets the gates based on the histogram profile. -
Page 11: Sensors
Sensors Technology Precise Coulter technology The Scepter™ 3.0 Sensors are microfluidic channels designed to replicate the Coulter Principle. Cell suspension is drawn into the reservoir channel and through the aperture sensing region. Increase in resistance causes an increase in voltage proportional to cell size (diameter). -
Page 12: Cell Counting
Cell Counting • Prepare dilutions in 1.5 mL microcentrifuge tubes. Other tubes may not be able to accommodate the width of the sensor, or provide sufficient sample depth for the instrument to function properly. • Use a diluting solution compatible with the characteristics of cells. The diluting solution used should not cause changes in the cell size and should have sufficient conductivity to enable operation of the instrument. -
Page 13: Counting Process
Counting Process Attach the Sensor, p .11 Perform before continuing with this section. 1. Immediately after mixing, submerge 01-Feb-18 14:16 01-Feb-18 14:16 01-Feb-18 14:16 Counting Process Counting Process Counting Process the tip of the sensor into the middle of Submerge the sample tube solution and press the Keep the Sensor Lift the Sensor... -
Page 14: Edit Analysis
Edit Analysis 01-Feb-18 14:16 01-Feb-18 14:16 01-Feb-18 14:16 1. Select Histogram from the main menu 01-Feb-18 14:16 Histogram: Edit Gates Histogram: Edit Gates Histogram Histogram and scroll to Edit Analysis, press OK. < Back Low gate will be highlighted and a Go to Home Edit Analysis flashing red line flanked by red arrows... -
Page 15: Exporting Data
Exporting Data Export to USB thumb drive 01-Feb-18 14:16 01-Feb-18 14:16 Histogram Histogram: Export 1. Select Histogram from the main < Back menu and scroll to Export to USB, Go to Home Insert Edit Analysis press the OK button. USB Thumb Drive Switch to Volume Into USB Port 2. -
Page 16: Reviewing Saved Data
Reviewing Saved Data Although Scepter™ 3.0 has the capacity to hold 999 histograms, we recommend exporting data for easier file Exporting Data, p .15. maintenance. See Select Histograms, press the OK button. 01-Feb-18 14:16 01-Feb-18 14:16 Menu Histogram The Histogram that was last edited or <... -
Page 17: Printing
Printing Before printing, the Scepter™ 3.0 must be paired to a printer with Bluetooth capablities ® (see Bluetooth Enabled Printer, p .7). Confirm the printer is ON and within signal range. ® 01-Feb-18 14:16 01-Feb-18 14:16 01-Feb-18 14:16 1. From the Main Menu, scroll to Menu Histogram Histogram... -
Page 18: Storage And Maintenance
Storage and Maintenance Storage • Store the Scepter™ 3.0 Cell Counter and Scepter™ 3.0 Sensors in a clean, dry environment at 15-30 °C. • Do not expose the instrument, sensors or charger to UV light. • Store Scepter™ 3.0 Test Beads in refrigerator after opening. Do not freeze. -
Page 19: Error Messages
Unable to reach website Not on the same network or Ensure the computer is on the same network as the Scepter 3.0. using the Scepter™ 3.0 network firewall setting If problem persists, check with your network administrator to... - Page 20 Message General Cause Corrective Action Concentration of sample Dilute sample so that it is no higher than recommended maximum Warning High concentration is too high operating range guideline. See Specifications, p .22. Sample volume too small, sensor not fully immersed Make sure sample volume is ≥...
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Page 21: Notice
Message General Cause Corrective Action Can not find file or Does not connect to Check or insert the “credential file” on USB thumb drive. credentials are incorrect Wi-Fi network Creating a Wi-Fi Configuration File, p .9 . ® ® No 2.4 GHz network(s) Go within range of a 2.4 GHz wireless network Network scan timeout within range... -
Page 22: Specifications
Specifications Scepter™ 3 .0 Instrument Body Acrylonitrile butadiene styrene (ABS) body, polycarbonate digital screen, Body Material silicone control buttons Height 28.321 cm (11.15 in.) Width 6.350 cm (2.5 in.) Depth 10.922 cm (4.3 in.) Weight (approximate) 229.5 g (0.5 lb) Wireless Connection Specifications Wireless (Wi-Fi , WLAN) -
Page 23: Product Ordering
Product Ordering Purchase products online at SigmaAldrich.com/products. Product Description Catalogue No. Scepter™ 3 .0 Handheld Automated Cell Counter Kit Includes Scepter™ 3.0 Handheld Automated Cell Counter, 50/pk of Scepter™ 3.0 Sensors (40 µm or 60 µm), Scepter™ 3.0 Test Beads, Charging Station and Mounting Kit, Quick Start Guide, and Safety information. with 40 µm sensors PHCC340KIT with 60 µm sensors... -
Page 24: Marks Of Conformity
Global Registration Marks of Conformity EUROPE TAIWAN TFB-1003 JAPAN SOUTH KOREA 注意 ！ 依據 低功率電波輻射性電機管理辦法 第十二條 R209-J00212 R-C-Emd-PHCC30000 經型式認證合格之低功率射頻電機， 非經許可， 公 RUSSIA, BELARUS HONG KONG 司、 商號或使用者均不得擅自變更頻率、 加大 功率或變更原設計之特性及功能。 第十四條 低功率射頻電機之使用不得影響飛航安全及干擾 合法通信 ； 經發現有干擾現象時， 應立即停用， 並改善至無干擾時方得繼續使用。 CHINA 前項合法通信， 指依電信法規定作業之無線電通 CMIIT ID 2020DJ2496 信。...
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